Experiment: KB with Mexicanolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0949 and Psyr_0950 are separated by 0 nucleotides Psyr_0950 and Psyr_0951 overlap by 16 nucleotides Psyr_0951 and Psyr_0952 overlap by 8 nucleotides Psyr_0952 and Psyr_0953 are separated by 118 nucleotides
Psyr_0949: Psyr_0949 - bacterial peptide chain release factor 1 (bRF-1), at 1,084,901 to 1,085,983
_0949
Psyr_0950: Psyr_0950 - [protein release factor]-glutamine N5-methyltransferase, at 1,085,984 to 1,086,817
_0950
Psyr_0951: Psyr_0951 - UBA/THIF-type NAD/FAD binding fold:MoeZ/MoeB, at 1,086,802 to 1,087,581
_0951
Psyr_0952: Psyr_0952 - glutamate racemase, at 1,087,574 to 1,088,389
_0952
Psyr_0953: Psyr_0953 - conserved hypothetical protein, at 1,088,508 to 1,089,026
_0953
Position (kb)
1086
1087
1088 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1085.948 kb on + strand at 1085.981 kb on + strand at 1085.981 kb on + strand at 1086.003 kb on + strand at 1086.838 kb on + strand at 1086.884 kb on + strand, within Psyr_0951 at 1086.884 kb on + strand, within Psyr_0951 at 1086.949 kb on + strand, within Psyr_0951 at 1087.064 kb on + strand, within Psyr_0951 at 1087.478 kb on + strand, within Psyr_0951 at 1087.478 kb on + strand, within Psyr_0951 at 1087.588 kb on + strand at 1087.588 kb on + strand at 1087.588 kb on + strand at 1087.588 kb on + strand at 1088.317 kb on - strand at 1088.424 kb on + strand at 1088.424 kb on + strand at 1088.425 kb on - strand at 1088.425 kb on - strand at 1088.425 kb on - strand at 1088.445 kb on + strand at 1088.445 kb on + strand at 1088.446 kb on - strand at 1088.446 kb on - strand at 1088.446 kb on - strand at 1088.446 kb on - strand at 1088.446 kb on - strand at 1088.447 kb on + strand at 1088.447 kb on + strand at 1088.447 kb on + strand at 1088.486 kb on + strand at 1088.491 kb on + strand at 1088.491 kb on + strand at 1088.491 kb on + strand at 1088.491 kb on + strand at 1088.492 kb on - strand at 1088.492 kb on - strand at 1088.492 kb on - strand at 1088.492 kb on - strand at 1088.495 kb on + strand at 1088.496 kb on - strand at 1088.496 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mexicanolide 10 mM remove 1,085,948 + -0.4 1,085,981 + -0.4 1,085,981 + -0.5 1,086,003 + -0.8 1,086,838 + -0.4 1,086,884 + Psyr_0951 0.11 -1.7 1,086,884 + Psyr_0951 0.11 -2.4 1,086,949 + Psyr_0951 0.19 -2.4 1,087,064 + Psyr_0951 0.34 -1.7 1,087,478 + Psyr_0951 0.87 -0.7 1,087,478 + Psyr_0951 0.87 -2.3 1,087,588 + -0.4 1,087,588 + -0.9 1,087,588 + -0.5 1,087,588 + -0.2 1,088,317 - -2.9 1,088,424 + -0.1 1,088,424 + +0.3 1,088,425 - -1.1 1,088,425 - -1.3 1,088,425 - -0.2 1,088,445 + -1.0 1,088,445 + +0.2 1,088,446 - +0.5 1,088,446 - -0.3 1,088,446 - +0.3 1,088,446 - -0.2 1,088,446 - -1.2 1,088,447 + +0.1 1,088,447 + -0.6 1,088,447 + -0.7 1,088,486 + +0.6 1,088,491 + -0.3 1,088,491 + -1.0 1,088,491 + -1.6 1,088,491 + -1.6 1,088,492 - +0.0 1,088,492 - +0.6 1,088,492 - -0.9 1,088,492 - -0.0 1,088,495 + +0.0 1,088,496 - +0.1 1,088,496 - +0.3
Or see this region's nucleotide sequence