Experiment: KB with Mexicanolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0391 and Psyr_0392 are separated by 112 nucleotides Psyr_0392 and Psyr_0393 overlap by 17 nucleotides Psyr_0393 and Psyr_0394 are separated by 107 nucleotides Psyr_0394 and Psyr_0395 are separated by 66 nucleotides
Psyr_0391: Psyr_0391 - Poly granule associated, at 419,054 to 419,746
_0391
Psyr_0392: Psyr_0392 - hypothetical protein, at 419,859 to 420,164
_0392
Psyr_0393: Psyr_0393 - Protein of unknown function DUF971, at 420,148 to 420,519
_0393
Psyr_0394: Psyr_0394 - Heat shock protein HslU, at 420,627 to 421,964
_0394
Psyr_0395: Psyr_0395 - HslV component of HslUV peptidase, Threonine peptidase, MEROPS family T01B, at 422,031 to 422,609
_0395
Position (kb)
420
421
422 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 419.696 kb on - strand at 419.747 kb on + strand at 419.747 kb on + strand at 419.748 kb on - strand at 419.769 kb on + strand at 419.769 kb on + strand at 419.770 kb on - strand at 419.770 kb on - strand at 419.796 kb on - strand at 419.868 kb on + strand at 419.868 kb on + strand at 419.869 kb on - strand at 419.869 kb on - strand at 419.869 kb on - strand at 419.945 kb on + strand, within Psyr_0392 at 419.946 kb on - strand, within Psyr_0392 at 419.988 kb on + strand, within Psyr_0392 at 419.988 kb on + strand, within Psyr_0392 at 419.988 kb on + strand, within Psyr_0392 at 419.989 kb on - strand, within Psyr_0392 at 419.989 kb on - strand, within Psyr_0392 at 419.989 kb on - strand, within Psyr_0392 at 420.018 kb on + strand, within Psyr_0392 at 420.018 kb on + strand, within Psyr_0392 at 420.018 kb on + strand, within Psyr_0392 at 420.018 kb on + strand, within Psyr_0392 at 420.018 kb on + strand, within Psyr_0392 at 420.018 kb on + strand, within Psyr_0392 at 420.019 kb on - strand, within Psyr_0392 at 420.019 kb on - strand, within Psyr_0392 at 420.019 kb on - strand, within Psyr_0392 at 420.218 kb on + strand, within Psyr_0393 at 420.219 kb on - strand, within Psyr_0393 at 420.219 kb on - strand, within Psyr_0393 at 420.219 kb on - strand, within Psyr_0393 at 420.219 kb on - strand, within Psyr_0393 at 420.264 kb on - strand, within Psyr_0393 at 420.264 kb on - strand, within Psyr_0393 at 420.264 kb on - strand, within Psyr_0393 at 420.264 kb on - strand, within Psyr_0393 at 420.320 kb on + strand, within Psyr_0393 at 420.320 kb on + strand, within Psyr_0393 at 420.321 kb on - strand, within Psyr_0393 at 420.321 kb on - strand, within Psyr_0393 at 420.321 kb on - strand, within Psyr_0393 at 420.464 kb on + strand, within Psyr_0393 at 420.493 kb on - strand at 420.493 kb on - strand at 420.540 kb on - strand at 420.540 kb on - strand at 420.612 kb on + strand at 420.613 kb on - strand at 420.613 kb on - strand at 420.615 kb on + strand at 420.615 kb on + strand at 420.615 kb on + strand at 420.615 kb on + strand at 420.615 kb on + strand at 420.616 kb on - strand at 420.616 kb on - strand at 420.616 kb on - strand at 420.616 kb on - strand at 420.616 kb on - strand at 420.616 kb on - strand at 420.635 kb on + strand at 420.635 kb on + strand at 420.635 kb on + strand at 420.635 kb on + strand at 420.636 kb on - strand at 420.636 kb on - strand at 420.636 kb on - strand at 420.636 kb on - strand at 420.637 kb on + strand at 420.637 kb on + strand at 420.680 kb on + strand at 420.688 kb on + strand at 420.688 kb on + strand at 420.688 kb on + strand at 420.689 kb on - strand at 420.689 kb on - strand at 420.908 kb on - strand, within Psyr_0394 at 421.175 kb on + strand, within Psyr_0394 at 421.175 kb on + strand, within Psyr_0394 at 421.175 kb on + strand, within Psyr_0394 at 421.176 kb on - strand, within Psyr_0394 at 421.680 kb on + strand, within Psyr_0394 at 421.681 kb on - strand, within Psyr_0394 at 421.696 kb on + strand, within Psyr_0394 at 421.697 kb on - strand, within Psyr_0394 at 421.825 kb on - strand, within Psyr_0394 at 421.825 kb on - strand, within Psyr_0394 at 421.919 kb on + strand at 421.920 kb on - strand at 421.985 kb on + strand at 422.446 kb on + strand, within Psyr_0395 at 422.447 kb on - strand, within Psyr_0395 at 422.458 kb on - strand, within Psyr_0395 at 422.458 kb on - strand, within Psyr_0395 at 422.458 kb on - strand, within Psyr_0395 at 422.528 kb on + strand, within Psyr_0395 at 422.628 kb on - strand at 422.665 kb on + strand at 422.666 kb on - strand at 422.666 kb on - strand at 422.687 kb on + strand at 422.842 kb on - strand at 422.842 kb on - strand at 422.842 kb on - strand at 422.901 kb on - strand at 422.901 kb on - strand at 422.932 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mexicanolide 10 mM remove 419,696 - -0.1 419,747 + -1.0 419,747 + -0.8 419,748 - +0.4 419,769 + +1.2 419,769 + -1.5 419,770 - -0.7 419,770 - +0.0 419,796 - +0.3 419,868 + -0.0 419,868 + +0.2 419,869 - +0.7 419,869 - +0.3 419,869 - +0.5 419,945 + Psyr_0392 0.28 +0.0 419,946 - Psyr_0392 0.28 -0.3 419,988 + Psyr_0392 0.42 -0.7 419,988 + Psyr_0392 0.42 +0.1 419,988 + Psyr_0392 0.42 -1.1 419,989 - Psyr_0392 0.42 +0.8 419,989 - Psyr_0392 0.42 +0.3 419,989 - Psyr_0392 0.42 +0.3 420,018 + Psyr_0392 0.52 -0.6 420,018 + Psyr_0392 0.52 +0.2 420,018 + Psyr_0392 0.52 -0.5 420,018 + Psyr_0392 0.52 -3.3 420,018 + Psyr_0392 0.52 -0.7 420,018 + Psyr_0392 0.52 -2.7 420,019 - Psyr_0392 0.52 -1.3 420,019 - Psyr_0392 0.52 -0.2 420,019 - Psyr_0392 0.52 -0.3 420,218 + Psyr_0393 0.19 -1.3 420,219 - Psyr_0393 0.19 -0.7 420,219 - Psyr_0393 0.19 -0.2 420,219 - Psyr_0393 0.19 +1.3 420,219 - Psyr_0393 0.19 +0.5 420,264 - Psyr_0393 0.31 +0.8 420,264 - Psyr_0393 0.31 +0.1 420,264 - Psyr_0393 0.31 +1.1 420,264 - Psyr_0393 0.31 -0.1 420,320 + Psyr_0393 0.46 -0.2 420,320 + Psyr_0393 0.46 -1.1 420,321 - Psyr_0393 0.47 -0.7 420,321 - Psyr_0393 0.47 +0.5 420,321 - Psyr_0393 0.47 +0.7 420,464 + Psyr_0393 0.85 +0.3 420,493 - +0.5 420,493 - +1.9 420,540 - +0.8 420,540 - +0.5 420,612 + -0.7 420,613 - +0.0 420,613 - +0.6 420,615 + -0.2 420,615 + -0.0 420,615 + +0.8 420,615 + -0.1 420,615 + +0.0 420,616 - +1.3 420,616 - -1.1 420,616 - +0.3 420,616 - -1.1 420,616 - +1.9 420,616 - -0.7 420,635 + +0.5 420,635 + -2.7 420,635 + -0.1 420,635 + -1.5 420,636 - -1.4 420,636 - +0.8 420,636 - -0.3 420,636 - +0.1 420,637 + +1.0 420,637 + -0.1 420,680 + -0.3 420,688 + -0.5 420,688 + -0.1 420,688 + -0.7 420,689 - +0.7 420,689 - -0.6 420,908 - Psyr_0394 0.21 -1.0 421,175 + Psyr_0394 0.41 -1.1 421,175 + Psyr_0394 0.41 -1.9 421,175 + Psyr_0394 0.41 -1.7 421,176 - Psyr_0394 0.41 -1.1 421,680 + Psyr_0394 0.79 +0.7 421,681 - Psyr_0394 0.79 -1.1 421,696 + Psyr_0394 0.80 -0.7 421,697 - Psyr_0394 0.80 +0.5 421,825 - Psyr_0394 0.90 +0.1 421,825 - Psyr_0394 0.90 -0.9 421,919 + +1.3 421,920 - -1.5 421,985 + -1.3 422,446 + Psyr_0395 0.72 +1.0 422,447 - Psyr_0395 0.72 -0.2 422,458 - Psyr_0395 0.74 -1.0 422,458 - Psyr_0395 0.74 -0.3 422,458 - Psyr_0395 0.74 -0.8 422,528 + Psyr_0395 0.86 -0.7 422,628 - -0.9 422,665 + -0.6 422,666 - +0.2 422,666 - -0.1 422,687 + +1.3 422,842 - +0.0 422,842 - -0.4 422,842 - +1.3 422,901 - +0.7 422,901 - -0.1 422,932 - -0.6
Or see this region's nucleotide sequence