Experiment: KB with Mexicanolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0258 and Psyr_0259 are separated by 151 nucleotides Psyr_0259 and Psyr_0260 are separated by 255 nucleotides Psyr_0260 and Psyr_0261 are separated by 224 nucleotides
Psyr_0258: Psyr_0258 - Two-component response regulator OmpR, at 274,133 to 274,873
_0258
Psyr_0259: Psyr_0259 - Osmolarity sensor protein envZ, at 275,025 to 276,347
_0259
Psyr_0260: Psyr_0260 - conserved hypothetical protein, at 276,603 to 277,070
_0260
Psyr_0261: Psyr_0261 - conserved hypothetical protein, at 277,295 to 277,582
_0261
Position (kb)
275
276
277 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 274.096 kb on + strand at 274.096 kb on + strand at 274.915 kb on + strand at 274.915 kb on + strand at 274.916 kb on - strand at 274.947 kb on + strand at 274.948 kb on - strand at 275.024 kb on + strand at 275.024 kb on + strand at 275.024 kb on + strand at 275.024 kb on + strand at 275.024 kb on + strand at 275.025 kb on - strand at 275.025 kb on - strand at 275.025 kb on - strand at 275.036 kb on + strand at 275.036 kb on + strand at 275.036 kb on + strand at 275.320 kb on - strand, within Psyr_0259 at 276.072 kb on + strand, within Psyr_0259 at 276.073 kb on - strand, within Psyr_0259 at 276.076 kb on + strand, within Psyr_0259 at 276.099 kb on + strand, within Psyr_0259 at 276.099 kb on + strand, within Psyr_0259 at 276.099 kb on + strand, within Psyr_0259 at 276.099 kb on + strand, within Psyr_0259 at 276.099 kb on + strand, within Psyr_0259 at 276.100 kb on - strand, within Psyr_0259 at 276.236 kb on + strand at 276.305 kb on - strand at 276.345 kb on + strand at 276.373 kb on + strand at 276.373 kb on + strand at 276.373 kb on + strand at 276.373 kb on + strand at 276.373 kb on + strand at 276.373 kb on + strand at 276.374 kb on - strand at 276.374 kb on - strand at 276.374 kb on - strand at 276.375 kb on + strand at 276.375 kb on + strand at 276.376 kb on - strand at 276.470 kb on + strand at 276.470 kb on + strand at 276.470 kb on + strand at 276.470 kb on + strand at 276.470 kb on + strand at 276.479 kb on + strand at 276.486 kb on - strand at 276.486 kb on - strand at 276.538 kb on + strand at 276.539 kb on - strand at 276.668 kb on + strand, within Psyr_0260 at 276.668 kb on + strand, within Psyr_0260 at 276.683 kb on - strand, within Psyr_0260 at 276.683 kb on - strand, within Psyr_0260 at 276.683 kb on - strand, within Psyr_0260 at 276.749 kb on - strand, within Psyr_0260 at 276.749 kb on - strand, within Psyr_0260 at 276.803 kb on + strand, within Psyr_0260 at 276.804 kb on - strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.835 kb on + strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 276.836 kb on - strand, within Psyr_0260 at 277.071 kb on + strand at 277.187 kb on + strand at 277.187 kb on + strand at 277.187 kb on + strand at 277.243 kb on + strand at 277.244 kb on - strand at 277.263 kb on + strand at 277.263 kb on + strand at 277.263 kb on + strand at 277.267 kb on - strand at 277.331 kb on + strand, within Psyr_0261 at 277.331 kb on + strand, within Psyr_0261 at 277.332 kb on - strand, within Psyr_0261 at 277.332 kb on - strand, within Psyr_0261 at 277.332 kb on - strand, within Psyr_0261
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mexicanolide 10 mM remove 274,096 + -0.2 274,096 + -0.7 274,915 + -0.6 274,915 + +1.0 274,916 - -1.0 274,947 + -0.4 274,948 - +0.6 275,024 + -1.6 275,024 + -0.8 275,024 + -0.8 275,024 + +0.1 275,024 + +0.3 275,025 - +0.1 275,025 - +0.1 275,025 - +0.2 275,036 + -0.9 275,036 + -0.7 275,036 + -1.8 275,320 - Psyr_0259 0.22 -1.7 276,072 + Psyr_0259 0.79 -2.4 276,073 - Psyr_0259 0.79 -0.7 276,076 + Psyr_0259 0.79 -0.0 276,099 + Psyr_0259 0.81 -1.3 276,099 + Psyr_0259 0.81 +0.2 276,099 + Psyr_0259 0.81 -1.5 276,099 + Psyr_0259 0.81 -2.0 276,099 + Psyr_0259 0.81 +0.6 276,100 - Psyr_0259 0.81 -0.4 276,236 + -2.1 276,305 - -1.7 276,345 + +0.1 276,373 + -1.5 276,373 + +1.4 276,373 + -0.2 276,373 + -1.6 276,373 + -1.4 276,373 + -0.2 276,374 - -0.8 276,374 - -0.3 276,374 - +0.2 276,375 + +0.9 276,375 + -0.3 276,376 - +1.3 276,470 + -0.0 276,470 + +0.2 276,470 + +0.0 276,470 + +1.1 276,470 + -0.9 276,479 + +0.3 276,486 - -0.5 276,486 - +0.9 276,538 + -0.4 276,539 - +0.9 276,668 + Psyr_0260 0.14 +0.6 276,668 + Psyr_0260 0.14 -0.8 276,683 - Psyr_0260 0.17 +1.3 276,683 - Psyr_0260 0.17 -0.3 276,683 - Psyr_0260 0.17 -0.5 276,749 - Psyr_0260 0.31 -0.3 276,749 - Psyr_0260 0.31 -0.5 276,803 + Psyr_0260 0.43 -0.8 276,804 - Psyr_0260 0.43 -0.2 276,835 + Psyr_0260 0.50 +0.1 276,835 + Psyr_0260 0.50 -0.0 276,835 + Psyr_0260 0.50 +0.4 276,835 + Psyr_0260 0.50 +0.1 276,835 + Psyr_0260 0.50 +0.7 276,835 + Psyr_0260 0.50 -0.0 276,835 + Psyr_0260 0.50 -0.1 276,835 + Psyr_0260 0.50 -0.2 276,835 + Psyr_0260 0.50 +0.2 276,836 - Psyr_0260 0.50 +0.6 276,836 - Psyr_0260 0.50 -0.7 276,836 - Psyr_0260 0.50 +0.3 276,836 - Psyr_0260 0.50 -0.7 276,836 - Psyr_0260 0.50 -0.2 276,836 - Psyr_0260 0.50 -0.3 276,836 - Psyr_0260 0.50 +2.6 276,836 - Psyr_0260 0.50 -0.6 276,836 - Psyr_0260 0.50 -0.6 277,071 + +0.1 277,187 + +0.2 277,187 + -0.5 277,187 + -1.3 277,243 + +0.2 277,244 - -0.3 277,263 + -0.2 277,263 + +1.9 277,263 + -1.4 277,267 - -0.4 277,331 + Psyr_0261 0.12 +0.4 277,331 + Psyr_0261 0.12 -0.3 277,332 - Psyr_0261 0.13 +0.0 277,332 - Psyr_0261 0.13 -0.4 277,332 - Psyr_0261 0.13 -0.8
Or see this region's nucleotide sequence