Experiment: KB with Mexicanolide 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0031 and Psyr_0032 are separated by 266 nucleotides Psyr_0032 and Psyr_0033 are separated by 125 nucleotides Psyr_0033 and Psyr_0034 overlap by 4 nucleotides
Psyr_0031: Psyr_0031 - Alpha/beta hydrolase fold protein, at 35,445 to 36,269
_0031
Psyr_0032: Psyr_0032 - conserved hypothetical protein, at 36,536 to 37,066
_0032
Psyr_0033: Psyr_0033 - tryptophan synthase, alpha chain, at 37,192 to 38,001
_0033
Psyr_0034: Psyr_0034 - tryptophan synthase, beta chain, at 37,998 to 39,227
_0034
Position (kb) 37
38
39 Strain fitness (log2 ratio) -2
-1
0
1
2 at 36.327 kb on + strand at 36.327 kb on + strand at 36.328 kb on - strand at 36.328 kb on - strand at 36.328 kb on - strand at 36.340 kb on + strand at 36.340 kb on + strand at 36.341 kb on - strand at 36.341 kb on - strand at 36.342 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.454 kb on + strand at 36.455 kb on - strand at 36.455 kb on - strand at 36.455 kb on - strand at 36.455 kb on - strand at 36.456 kb on + strand at 36.564 kb on + strand at 36.564 kb on + strand at 36.633 kb on + strand, within Psyr_0032 at 36.633 kb on + strand, within Psyr_0032 at 36.633 kb on + strand, within Psyr_0032 at 36.633 kb on + strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.634 kb on - strand, within Psyr_0032 at 36.645 kb on + strand, within Psyr_0032 at 36.646 kb on - strand, within Psyr_0032 at 36.713 kb on + strand, within Psyr_0032 at 36.713 kb on + strand, within Psyr_0032 at 36.713 kb on + strand, within Psyr_0032 at 36.714 kb on - strand, within Psyr_0032 at 36.714 kb on - strand, within Psyr_0032 at 36.902 kb on - strand, within Psyr_0032 at 37.045 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.140 kb on + strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.141 kb on - strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.144 kb on + strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.145 kb on - strand at 37.176 kb on - strand at 37.207 kb on - strand at 37.207 kb on - strand at 37.210 kb on + strand at 37.210 kb on + strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.211 kb on - strand at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.475 kb on + strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.476 kb on - strand, within Psyr_0033 at 37.478 kb on + strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.695 kb on - strand, within Psyr_0033 at 37.697 kb on + strand, within Psyr_0033 at 37.991 kb on - strand at 37.991 kb on - strand at 38.002 kb on - strand at 38.234 kb on - strand, within Psyr_0034 at 38.234 kb on - strand, within Psyr_0034 at 38.234 kb on - strand, within Psyr_0034 at 38.350 kb on + strand, within Psyr_0034 at 38.351 kb on - strand, within Psyr_0034 at 38.351 kb on - strand, within Psyr_0034 at 38.351 kb on - strand, within Psyr_0034 at 38.648 kb on - strand, within Psyr_0034 at 38.806 kb on + strand, within Psyr_0034 at 38.806 kb on + strand, within Psyr_0034 at 38.806 kb on + strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.807 kb on - strand, within Psyr_0034 at 38.920 kb on - strand, within Psyr_0034
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Mexicanolide 10 mM remove 36,327 + -1.3 36,327 + +0.2 36,328 - -0.7 36,328 - -0.4 36,328 - -1.6 36,340 + -0.4 36,340 + -0.0 36,341 - -0.5 36,341 - -0.2 36,342 + +0.5 36,454 + -1.2 36,454 + -0.7 36,454 + -2.4 36,454 + -0.2 36,454 + +0.1 36,454 + -1.1 36,454 + -0.0 36,454 + -0.5 36,454 + +0.5 36,454 + -0.3 36,454 + -0.5 36,455 - +1.0 36,455 - -0.2 36,455 - -0.0 36,455 - +0.1 36,456 + -0.8 36,564 + -0.4 36,564 + -0.7 36,633 + Psyr_0032 0.18 +0.5 36,633 + Psyr_0032 0.18 -0.0 36,633 + Psyr_0032 0.18 +0.8 36,633 + Psyr_0032 0.18 +0.3 36,634 - Psyr_0032 0.18 -0.8 36,634 - Psyr_0032 0.18 -0.7 36,634 - Psyr_0032 0.18 +0.7 36,634 - Psyr_0032 0.18 +0.9 36,634 - Psyr_0032 0.18 -0.4 36,645 + Psyr_0032 0.21 +0.1 36,646 - Psyr_0032 0.21 +1.1 36,713 + Psyr_0032 0.33 +0.7 36,713 + Psyr_0032 0.33 +0.0 36,713 + Psyr_0032 0.33 -0.3 36,714 - Psyr_0032 0.34 +0.7 36,714 - Psyr_0032 0.34 -1.7 36,902 - Psyr_0032 0.69 -0.2 37,045 + +0.6 37,140 + +0.3 37,140 + -2.0 37,140 + +0.1 37,140 + -0.6 37,140 + +0.2 37,140 + -0.1 37,140 + +0.2 37,140 + +0.1 37,140 + +1.3 37,140 + +0.0 37,140 + +0.0 37,141 - +0.6 37,141 - -0.7 37,141 - -1.2 37,141 - -0.3 37,141 - -0.2 37,141 - -0.0 37,141 - +0.2 37,141 - +0.1 37,141 - +0.3 37,141 - +0.3 37,144 + -0.5 37,144 + -0.3 37,144 + -0.8 37,144 + -0.7 37,144 + -0.5 37,144 + +0.2 37,144 + -0.1 37,145 - -0.9 37,145 - +0.1 37,145 - -2.2 37,145 - +2.2 37,145 - -0.8 37,145 - +0.2 37,145 - -1.8 37,145 - -0.1 37,145 - +0.6 37,176 - +0.4 37,207 - -0.6 37,207 - +0.7 37,210 + -0.3 37,210 + +0.3 37,211 - -1.5 37,211 - -0.1 37,211 - +0.3 37,211 - -0.2 37,211 - -0.7 37,475 + Psyr_0033 0.35 -1.4 37,475 + Psyr_0033 0.35 +0.3 37,475 + Psyr_0033 0.35 -0.7 37,475 + Psyr_0033 0.35 +1.3 37,476 - Psyr_0033 0.35 +1.3 37,476 - Psyr_0033 0.35 +1.3 37,476 - Psyr_0033 0.35 +1.3 37,478 + Psyr_0033 0.35 +1.3 37,695 - Psyr_0033 0.62 -0.7 37,695 - Psyr_0033 0.62 +1.3 37,695 - Psyr_0033 0.62 +1.3 37,695 - Psyr_0033 0.62 -1.8 37,697 + Psyr_0033 0.62 +1.3 37,991 - +1.3 37,991 - -1.5 38,002 - +0.6 38,234 - Psyr_0034 0.19 +0.3 38,234 - Psyr_0034 0.19 -0.2 38,234 - Psyr_0034 0.19 +1.3 38,350 + Psyr_0034 0.29 +0.3 38,351 - Psyr_0034 0.29 -0.2 38,351 - Psyr_0034 0.29 +1.3 38,351 - Psyr_0034 0.29 -0.2 38,648 - Psyr_0034 0.53 +0.3 38,806 + Psyr_0034 0.66 +1.3 38,806 + Psyr_0034 0.66 +0.3 38,806 + Psyr_0034 0.66 -1.0 38,807 - Psyr_0034 0.66 +1.3 38,807 - Psyr_0034 0.66 +1.3 38,807 - Psyr_0034 0.66 +1.3 38,807 - Psyr_0034 0.66 -0.2 38,807 - Psyr_0034 0.66 +0.0 38,807 - Psyr_0034 0.66 +1.3 38,920 - Psyr_0034 0.75 -0.2
Or see this region's nucleotide sequence