Experiment: LB; anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secD-2 and yajC are separated by 17 nucleotides yajC and tgt are separated by 26 nucleotides tgt and queA are separated by 161 nucleotides
SO3111: secD-2 - protein-export membrane protein SecD (NCBI ptt file), at 3,239,490 to 3,241,340
secD-2
SO3112: yajC - preprotein translocase, YajC subunit (NCBI ptt file), at 3,241,358 to 3,241,690
yajC
SO3113: tgt - queuine tRNA-ribosyltransferase (NCBI ptt file), at 3,241,717 to 3,242,841
tgt
SO3114: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI ptt file), at 3,243,003 to 3,244,040
queA
Position (kb)
3241
3242
3243 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3240.750 kb on - strand, within secD-2 at 3240.751 kb on - strand, within secD-2 at 3241.007 kb on + strand, within secD-2 at 3241.153 kb on + strand, within secD-2 at 3241.175 kb on - strand at 3241.196 kb on - strand at 3241.214 kb on - strand at 3241.342 kb on + strand at 3241.350 kb on - strand at 3241.350 kb on - strand at 3241.377 kb on - strand at 3241.651 kb on - strand, within yajC at 3241.651 kb on - strand, within yajC at 3241.913 kb on - strand, within tgt at 3242.050 kb on + strand, within tgt at 3242.071 kb on + strand, within tgt at 3242.236 kb on - strand, within tgt at 3242.286 kb on - strand, within tgt at 3242.320 kb on + strand, within tgt at 3242.345 kb on + strand, within tgt at 3242.364 kb on + strand, within tgt at 3242.427 kb on + strand, within tgt at 3242.663 kb on - strand, within tgt at 3242.669 kb on + strand, within tgt at 3242.669 kb on + strand, within tgt at 3242.677 kb on - strand, within tgt at 3242.722 kb on - strand, within tgt at 3242.724 kb on - strand, within tgt at 3242.964 kb on + strand at 3242.964 kb on + strand at 3243.226 kb on + strand, within queA at 3243.226 kb on + strand, within queA at 3243.260 kb on - strand, within queA at 3243.353 kb on + strand, within queA at 3243.545 kb on - strand, within queA at 3243.568 kb on - strand, within queA at 3243.612 kb on - strand, within queA at 3243.627 kb on - strand, within queA at 3243.629 kb on + strand, within queA at 3243.751 kb on - strand, within queA at 3243.766 kb on + strand, within queA at 3243.825 kb on - strand, within queA
Per-strain Table
Position Strand Gene LocusTag Fraction LB; anaerobic remove 3,240,750 - secD-2 SO3111 0.68 -1.4 3,240,751 - secD-2 SO3111 0.68 +0.2 3,241,007 + secD-2 SO3111 0.82 -0.8 3,241,153 + secD-2 SO3111 0.90 -0.7 3,241,175 - -0.3 3,241,196 - -0.8 3,241,214 - +0.5 3,241,342 + +1.8 3,241,350 - -2.4 3,241,350 - -1.5 3,241,377 - -2.0 3,241,651 - yajC SO3112 0.88 -1.6 3,241,651 - yajC SO3112 0.88 -1.4 3,241,913 - tgt SO3113 0.17 -1.9 3,242,050 + tgt SO3113 0.30 -1.7 3,242,071 + tgt SO3113 0.31 -1.5 3,242,236 - tgt SO3113 0.46 -2.7 3,242,286 - tgt SO3113 0.51 -0.3 3,242,320 + tgt SO3113 0.54 -2.6 3,242,345 + tgt SO3113 0.56 -2.2 3,242,364 + tgt SO3113 0.58 -1.3 3,242,427 + tgt SO3113 0.63 -2.3 3,242,663 - tgt SO3113 0.84 -1.3 3,242,669 + tgt SO3113 0.85 -2.2 3,242,669 + tgt SO3113 0.85 -1.3 3,242,677 - tgt SO3113 0.85 +1.0 3,242,722 - tgt SO3113 0.89 -1.3 3,242,724 - tgt SO3113 0.90 +0.5 3,242,964 + -1.9 3,242,964 + +0.0 3,243,226 + queA SO3114 0.21 +0.5 3,243,226 + queA SO3114 0.21 +1.1 3,243,260 - queA SO3114 0.25 +1.5 3,243,353 + queA SO3114 0.34 -2.1 3,243,545 - queA SO3114 0.52 -2.5 3,243,568 - queA SO3114 0.54 +0.3 3,243,612 - queA SO3114 0.59 -1.4 3,243,627 - queA SO3114 0.60 -1.7 3,243,629 + queA SO3114 0.60 +0.1 3,243,751 - queA SO3114 0.72 -0.8 3,243,766 + queA SO3114 0.74 +0.6 3,243,825 - queA SO3114 0.79 +0.2
Or see this region's nucleotide sequence