Experiment: KB with 2',5'-Dihydroxy-4-Methoxychalcone 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0307 and Psyr_0308 are separated by 288 nucleotides Psyr_0308 and Psyr_0309 are separated by 47 nucleotides Psyr_0309 and Psyr_0310 overlap by 11 nucleotides
Psyr_0307: Psyr_0307 - hypothetical protein, at 327,895 to 328,398
_0307
Psyr_0308: Psyr_0308 - hypothetical protein, at 328,687 to 329,424
_0308
Psyr_0309: Psyr_0309 - Colicin immunity protein/pyocin immunity protein, at 329,472 to 329,744
_0309
Psyr_0310: Psyr_0310 - HNH endonuclease:S-type Pyocin, at 329,734 to 331,704
_0310
Position (kb) 328
329
330 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 327.779 kb on + strand at 327.779 kb on + strand at 327.806 kb on + strand at 327.806 kb on + strand at 327.807 kb on - strand at 327.813 kb on + strand at 327.813 kb on + strand at 327.814 kb on - strand at 327.837 kb on - strand at 327.837 kb on - strand at 327.842 kb on - strand at 327.931 kb on - strand at 327.996 kb on - strand, within Psyr_0307 at 328.012 kb on - strand, within Psyr_0307 at 328.029 kb on - strand, within Psyr_0307 at 328.044 kb on + strand, within Psyr_0307 at 328.047 kb on + strand, within Psyr_0307 at 328.047 kb on + strand, within Psyr_0307 at 328.117 kb on + strand, within Psyr_0307 at 328.121 kb on + strand, within Psyr_0307 at 328.178 kb on - strand, within Psyr_0307 at 328.214 kb on + strand, within Psyr_0307 at 328.214 kb on + strand at 328.214 kb on + strand, within Psyr_0307 at 328.257 kb on + strand, within Psyr_0307 at 328.257 kb on + strand, within Psyr_0307 at 328.257 kb on + strand, within Psyr_0307 at 328.258 kb on - strand, within Psyr_0307 at 328.258 kb on - strand, within Psyr_0307 at 328.294 kb on + strand, within Psyr_0307 at 328.295 kb on - strand, within Psyr_0307 at 328.321 kb on - strand, within Psyr_0307 at 328.362 kb on + strand at 328.363 kb on - strand at 328.363 kb on - strand at 328.392 kb on + strand at 328.411 kb on - strand at 328.424 kb on + strand at 328.439 kb on + strand at 328.439 kb on + strand at 328.440 kb on - strand at 328.440 kb on - strand at 328.440 kb on - strand at 328.443 kb on + strand at 328.443 kb on + strand at 328.444 kb on - strand at 328.444 kb on - strand at 328.467 kb on - strand at 328.472 kb on - strand at 328.510 kb on - strand at 328.531 kb on - strand at 328.575 kb on - strand at 328.575 kb on - strand at 328.575 kb on - strand at 328.575 kb on - strand at 328.583 kb on + strand at 328.583 kb on + strand at 328.584 kb on - strand at 328.613 kb on + strand at 328.614 kb on - strand at 328.704 kb on + strand at 328.704 kb on + strand at 328.705 kb on - strand at 328.966 kb on + strand, within Psyr_0308 at 328.966 kb on + strand, within Psyr_0308 at 328.966 kb on + strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 328.967 kb on - strand, within Psyr_0308 at 329.098 kb on + strand, within Psyr_0308 at 329.098 kb on + strand, within Psyr_0308 at 329.098 kb on + strand, within Psyr_0308 at 329.098 kb on + strand, within Psyr_0308 at 329.098 kb on + strand, within Psyr_0308 at 329.099 kb on - strand, within Psyr_0308 at 329.101 kb on + strand, within Psyr_0308 at 329.101 kb on + strand, within Psyr_0308 at 329.113 kb on + strand, within Psyr_0308 at 329.113 kb on + strand, within Psyr_0308 at 329.113 kb on + strand, within Psyr_0308 at 329.114 kb on - strand, within Psyr_0308 at 329.114 kb on - strand, within Psyr_0308 at 329.114 kb on - strand, within Psyr_0308 at 329.114 kb on - strand, within Psyr_0308 at 329.114 kb on - strand, within Psyr_0308 at 329.168 kb on + strand, within Psyr_0308 at 329.168 kb on + strand, within Psyr_0308 at 329.168 kb on + strand, within Psyr_0308 at 329.169 kb on - strand, within Psyr_0308 at 329.169 kb on - strand, within Psyr_0308 at 329.221 kb on + strand, within Psyr_0308 at 329.221 kb on + strand, within Psyr_0308 at 329.221 kb on + strand, within Psyr_0308 at 329.221 kb on + strand, within Psyr_0308 at 329.222 kb on - strand, within Psyr_0308 at 329.238 kb on + strand, within Psyr_0308 at 329.254 kb on - strand, within Psyr_0308 at 329.254 kb on - strand, within Psyr_0308 at 329.256 kb on - strand, within Psyr_0308 at 329.308 kb on + strand, within Psyr_0308 at 329.308 kb on + strand, within Psyr_0308 at 329.308 kb on + strand, within Psyr_0308 at 329.308 kb on + strand, within Psyr_0308 at 329.309 kb on - strand, within Psyr_0308 at 329.309 kb on - strand, within Psyr_0308 at 329.330 kb on - strand, within Psyr_0308 at 329.330 kb on - strand, within Psyr_0308 at 329.333 kb on + strand, within Psyr_0308 at 329.334 kb on - strand, within Psyr_0308 at 329.334 kb on - strand, within Psyr_0308 at 329.334 kb on - strand, within Psyr_0308 at 329.334 kb on - strand, within Psyr_0308 at 329.334 kb on - strand, within Psyr_0308 at 329.361 kb on - strand at 329.381 kb on - strand at 329.381 kb on - strand at 329.381 kb on - strand at 329.392 kb on + strand at 329.413 kb on + strand at 329.414 kb on - strand at 329.414 kb on - strand at 329.571 kb on + strand, within Psyr_0309 at 329.717 kb on - strand at 329.769 kb on + strand at 329.770 kb on - strand at 329.770 kb on - strand at 329.811 kb on - strand at 329.917 kb on + strand at 329.918 kb on - strand at 329.929 kb on - strand at 330.158 kb on + strand, within Psyr_0310 at 330.158 kb on + strand, within Psyr_0310 at 330.158 kb on + strand, within Psyr_0310 at 330.159 kb on - strand, within Psyr_0310 at 330.159 kb on - strand, within Psyr_0310 at 330.159 kb on - strand, within Psyr_0310 at 330.159 kb on - strand, within Psyr_0310 at 330.159 kb on - strand, within Psyr_0310 at 330.162 kb on + strand, within Psyr_0310 at 330.163 kb on - strand, within Psyr_0310 at 330.163 kb on - strand, within Psyr_0310 at 330.163 kb on - strand, within Psyr_0310 at 330.200 kb on + strand, within Psyr_0310 at 330.200 kb on + strand, within Psyr_0310 at 330.200 kb on + strand, within Psyr_0310 at 330.201 kb on - strand, within Psyr_0310 at 330.201 kb on - strand, within Psyr_0310 at 330.201 kb on - strand, within Psyr_0310 at 330.201 kb on - strand, within Psyr_0310 at 330.233 kb on + strand, within Psyr_0310 at 330.234 kb on - strand, within Psyr_0310 at 330.250 kb on + strand, within Psyr_0310 at 330.280 kb on + strand, within Psyr_0310 at 330.281 kb on - strand, within Psyr_0310 at 330.337 kb on + strand, within Psyr_0310 at 330.338 kb on - strand, within Psyr_0310 at 330.390 kb on - strand, within Psyr_0310 at 330.410 kb on + strand, within Psyr_0310 at 330.410 kb on + strand, within Psyr_0310 at 330.410 kb on + strand, within Psyr_0310 at 330.410 kb on + strand, within Psyr_0310 at 330.410 kb on + strand, within Psyr_0310 at 330.410 kb on + strand, within Psyr_0310 at 330.410 kb on + strand, within Psyr_0310 at 330.410 kb on + strand, within Psyr_0310 at 330.411 kb on - strand, within Psyr_0310 at 330.411 kb on - strand, within Psyr_0310 at 330.411 kb on - strand, within Psyr_0310 at 330.411 kb on - strand, within Psyr_0310 at 330.411 kb on - strand, within Psyr_0310 at 330.411 kb on - strand, within Psyr_0310 at 330.411 kb on - strand, within Psyr_0310 at 330.411 kb on - strand, within Psyr_0310 at 330.411 kb on - strand, within Psyr_0310 at 330.411 kb on - strand, within Psyr_0310
Per-strain Table
Position Strand Gene LocusTag Fraction KB with 2',5'-Dihydroxy-4-Methoxychalcone 10 mM remove 327,779 + -1.2 327,779 + -0.1 327,806 + +0.5 327,806 + -0.7 327,807 - +0.3 327,813 + -1.5 327,813 + -0.6 327,814 - +1.1 327,837 - -0.0 327,837 - -0.4 327,842 - -0.6 327,931 - +0.4 327,996 - Psyr_0307 0.20 -1.1 328,012 - Psyr_0307 0.23 -0.4 328,029 - Psyr_0307 0.27 -0.8 328,044 + Psyr_0307 0.30 -1.6 328,047 + Psyr_0307 0.30 -2.0 328,047 + Psyr_0307 0.30 +0.2 328,117 + Psyr_0307 0.44 -0.0 328,121 + Psyr_0307 0.45 +0.7 328,178 - Psyr_0307 0.56 -0.7 328,214 + Psyr_0307 0.63 -1.7 328,214 + -0.7 328,214 + Psyr_0307 0.63 -0.7 328,257 + Psyr_0307 0.72 +0.3 328,257 + Psyr_0307 0.72 -0.5 328,257 + Psyr_0307 0.72 -0.5 328,258 - Psyr_0307 0.72 -0.2 328,258 - Psyr_0307 0.72 +0.4 328,294 + Psyr_0307 0.79 -0.3 328,295 - Psyr_0307 0.79 -0.3 328,321 - Psyr_0307 0.85 -0.7 328,362 + -0.3 328,363 - +0.2 328,363 - +0.4 328,392 + -0.8 328,411 - +0.7 328,424 + +0.4 328,439 + -0.0 328,439 + +0.9 328,440 - +0.4 328,440 - +3.3 328,440 - +1.1 328,443 + -0.4 328,443 + +0.5 328,444 - -0.7 328,444 - -0.8 328,467 - -0.0 328,472 - -0.7 328,510 - +0.0 328,531 - +0.4 328,575 - +0.5 328,575 - -2.9 328,575 - +0.1 328,575 - -1.5 328,583 + -0.8 328,583 + +0.2 328,584 - -0.5 328,613 + +0.3 328,614 - -0.4 328,704 + -0.4 328,704 + +0.9 328,705 - +0.9 328,966 + Psyr_0308 0.38 +1.3 328,966 + Psyr_0308 0.38 -0.2 328,966 + Psyr_0308 0.38 -0.5 328,967 - Psyr_0308 0.38 -0.2 328,967 - Psyr_0308 0.38 +1.4 328,967 - Psyr_0308 0.38 +0.3 328,967 - Psyr_0308 0.38 -0.1 328,967 - Psyr_0308 0.38 +0.2 328,967 - Psyr_0308 0.38 +0.2 328,967 - Psyr_0308 0.38 +0.3 329,098 + Psyr_0308 0.56 +0.4 329,098 + Psyr_0308 0.56 +0.9 329,098 + Psyr_0308 0.56 +0.9 329,098 + Psyr_0308 0.56 -1.4 329,098 + Psyr_0308 0.56 -0.2 329,099 - Psyr_0308 0.56 +0.2 329,101 + Psyr_0308 0.56 +0.4 329,101 + Psyr_0308 0.56 -0.5 329,113 + Psyr_0308 0.58 +0.3 329,113 + Psyr_0308 0.58 +0.0 329,113 + Psyr_0308 0.58 -1.5 329,114 - Psyr_0308 0.58 -0.0 329,114 - Psyr_0308 0.58 -0.0 329,114 - Psyr_0308 0.58 -0.5 329,114 - Psyr_0308 0.58 +0.1 329,114 - Psyr_0308 0.58 -0.0 329,168 + Psyr_0308 0.65 -0.3 329,168 + Psyr_0308 0.65 -0.5 329,168 + Psyr_0308 0.65 +0.0 329,169 - Psyr_0308 0.65 -1.5 329,169 - Psyr_0308 0.65 -0.1 329,221 + Psyr_0308 0.72 +1.2 329,221 + Psyr_0308 0.72 +0.7 329,221 + Psyr_0308 0.72 -2.9 329,221 + Psyr_0308 0.72 -1.2 329,222 - Psyr_0308 0.72 -0.0 329,238 + Psyr_0308 0.75 +0.1 329,254 - Psyr_0308 0.77 -0.3 329,254 - Psyr_0308 0.77 +0.8 329,256 - Psyr_0308 0.77 +0.8 329,308 + Psyr_0308 0.84 -0.8 329,308 + Psyr_0308 0.84 +0.5 329,308 + Psyr_0308 0.84 -1.2 329,308 + Psyr_0308 0.84 +0.8 329,309 - Psyr_0308 0.84 +1.4 329,309 - Psyr_0308 0.84 +0.0 329,330 - Psyr_0308 0.87 +0.8 329,330 - Psyr_0308 0.87 -1.5 329,333 + Psyr_0308 0.88 -0.3 329,334 - Psyr_0308 0.88 -1.7 329,334 - Psyr_0308 0.88 +0.9 329,334 - Psyr_0308 0.88 +0.5 329,334 - Psyr_0308 0.88 -0.6 329,334 - Psyr_0308 0.88 +0.7 329,361 - +0.5 329,381 - -0.3 329,381 - -0.4 329,381 - +0.7 329,392 + +0.2 329,413 + -2.5 329,414 - +0.5 329,414 - +0.5 329,571 + Psyr_0309 0.36 -1.1 329,717 - -0.4 329,769 + -0.5 329,770 - +0.1 329,770 - -1.3 329,811 - +0.8 329,917 + -0.2 329,918 - -0.7 329,929 - +0.7 330,158 + Psyr_0310 0.22 +0.3 330,158 + Psyr_0310 0.22 -0.5 330,158 + Psyr_0310 0.22 +1.0 330,159 - Psyr_0310 0.22 -0.3 330,159 - Psyr_0310 0.22 -0.6 330,159 - Psyr_0310 0.22 +0.4 330,159 - Psyr_0310 0.22 +1.7 330,159 - Psyr_0310 0.22 -1.6 330,162 + Psyr_0310 0.22 -1.4 330,163 - Psyr_0310 0.22 +2.0 330,163 - Psyr_0310 0.22 -0.6 330,163 - Psyr_0310 0.22 -0.4 330,200 + Psyr_0310 0.24 +0.7 330,200 + Psyr_0310 0.24 +1.3 330,200 + Psyr_0310 0.24 -0.5 330,201 - Psyr_0310 0.24 +0.3 330,201 - Psyr_0310 0.24 -0.3 330,201 - Psyr_0310 0.24 -0.4 330,201 - Psyr_0310 0.24 -0.9 330,233 + Psyr_0310 0.25 +0.7 330,234 - Psyr_0310 0.25 -0.1 330,250 + Psyr_0310 0.26 -0.5 330,280 + Psyr_0310 0.28 -0.4 330,281 - Psyr_0310 0.28 -0.8 330,337 + Psyr_0310 0.31 +0.2 330,338 - Psyr_0310 0.31 -0.2 330,390 - Psyr_0310 0.33 -0.4 330,410 + Psyr_0310 0.34 -0.1 330,410 + Psyr_0310 0.34 +0.9 330,410 + Psyr_0310 0.34 +0.6 330,410 + Psyr_0310 0.34 +0.0 330,410 + Psyr_0310 0.34 +0.3 330,410 + Psyr_0310 0.34 +0.3 330,410 + Psyr_0310 0.34 +1.0 330,410 + Psyr_0310 0.34 -0.6 330,411 - Psyr_0310 0.34 +0.2 330,411 - Psyr_0310 0.34 -0.0 330,411 - Psyr_0310 0.34 -0.2 330,411 - Psyr_0310 0.34 -0.3 330,411 - Psyr_0310 0.34 +1.6 330,411 - Psyr_0310 0.34 -0.6 330,411 - Psyr_0310 0.34 +0.4 330,411 - Psyr_0310 0.34 -1.9 330,411 - Psyr_0310 0.34 +0.2 330,411 - Psyr_0310 0.34 -0.2
Or see this region's nucleotide sequence