Experiment: KB with Isosafrole 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1550 and Psyr_1551 are separated by 28 nucleotides Psyr_1551 and Psyr_RNA25 are separated by 92 nucleotides Psyr_RNA25 and Psyr_1552 are separated by 58 nucleotides Psyr_1552 and Psyr_1553 are separated by 602 nucleotides
Psyr_1550: Psyr_1550 - Beta-lactamase-like protein, at 1,743,360 to 1,744,118
_1550
Psyr_1551: Psyr_1551 - phosphoribosylaminoimidazole-succinocarboxamide synthase, at 1,744,147 to 1,744,857
_1551
Psyr_RNA25: Psyr_RNA25 - tRNA-Ser, at 1,744,950 to 1,745,039
_RNA25
Psyr_1552: Psyr_1552 - CDS, at 1,745,098 to 1,745,496
_1552
Psyr_1553: Psyr_1553 - hypothetical protein, at 1,746,099 to 1,746,434
_1553
Position (kb)
1745
1746 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1744.109 kb on + strand at 1744.125 kb on + strand at 1744.125 kb on + strand at 1744.125 kb on + strand at 1744.125 kb on + strand at 1744.125 kb on + strand at 1744.129 kb on + strand at 1744.129 kb on + strand at 1744.129 kb on + strand at 1744.129 kb on + strand at 1744.934 kb on + strand at 1744.996 kb on + strand, within Psyr_RNA25 at 1745.012 kb on + strand, within Psyr_RNA25 at 1745.040 kb on + strand at 1745.046 kb on + strand at 1745.047 kb on - strand at 1745.069 kb on + strand at 1745.080 kb on + strand at 1745.092 kb on + strand at 1745.113 kb on + strand at 1745.114 kb on - strand at 1745.187 kb on + strand, within Psyr_1552 at 1745.187 kb on + strand, within Psyr_1552 at 1745.187 kb on + strand, within Psyr_1552 at 1745.187 kb on + strand, within Psyr_1552 at 1745.187 kb on + strand, within Psyr_1552 at 1745.187 kb on + strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.188 kb on - strand, within Psyr_1552 at 1745.262 kb on + strand, within Psyr_1552 at 1745.262 kb on + strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.263 kb on - strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.269 kb on + strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.270 kb on - strand, within Psyr_1552 at 1745.278 kb on - strand, within Psyr_1552 at 1745.316 kb on + strand, within Psyr_1552 at 1745.316 kb on + strand, within Psyr_1552 at 1745.317 kb on - strand, within Psyr_1552 at 1745.317 kb on - strand, within Psyr_1552 at 1745.317 kb on - strand, within Psyr_1552 at 1745.498 kb on + strand at 1745.498 kb on + strand at 1745.499 kb on - strand at 1745.499 kb on - strand at 1745.499 kb on - strand at 1745.551 kb on - strand at 1745.551 kb on - strand at 1745.551 kb on - strand at 1745.551 kb on - strand at 1745.584 kb on + strand at 1745.613 kb on + strand at 1745.613 kb on + strand at 1745.614 kb on - strand at 1745.655 kb on + strand at 1745.655 kb on + strand at 1745.700 kb on + strand at 1745.785 kb on - strand at 1745.875 kb on - strand at 1745.901 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.950 kb on + strand at 1745.951 kb on - strand at 1745.951 kb on - strand at 1745.951 kb on - strand at 1745.951 kb on - strand at 1745.951 kb on - strand at 1746.053 kb on - strand at 1746.056 kb on - strand at 1746.056 kb on - strand at 1746.056 kb on - strand at 1746.056 kb on - strand at 1746.087 kb on + strand at 1746.110 kb on + strand at 1746.158 kb on + strand, within Psyr_1553 at 1746.200 kb on + strand, within Psyr_1553 at 1746.200 kb on + strand, within Psyr_1553 at 1746.201 kb on - strand, within Psyr_1553 at 1746.201 kb on - strand, within Psyr_1553 at 1746.292 kb on + strand, within Psyr_1553 at 1746.348 kb on - strand, within Psyr_1553 at 1746.348 kb on - strand, within Psyr_1553 at 1746.351 kb on + strand, within Psyr_1553 at 1746.351 kb on + strand, within Psyr_1553 at 1746.351 kb on + strand, within Psyr_1553 at 1746.352 kb on - strand, within Psyr_1553 at 1746.352 kb on - strand, within Psyr_1553 at 1746.352 kb on - strand, within Psyr_1553 at 1746.352 kb on - strand, within Psyr_1553 at 1746.354 kb on - strand, within Psyr_1553 at 1746.422 kb on + strand at 1746.423 kb on - strand at 1746.459 kb on + strand at 1746.470 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Isosafrole 10 mM remove 1,744,109 + +0.3 1,744,125 + -0.2 1,744,125 + +1.8 1,744,125 + +0.1 1,744,125 + +0.0 1,744,125 + +0.7 1,744,129 + -1.3 1,744,129 + -0.2 1,744,129 + +0.3 1,744,129 + -1.1 1,744,934 + -0.5 1,744,996 + Psyr_RNA25 0.51 -2.1 1,745,012 + Psyr_RNA25 0.69 +2.0 1,745,040 + +0.8 1,745,046 + +0.4 1,745,047 - -1.7 1,745,069 + -0.9 1,745,080 + -0.1 1,745,092 + -0.1 1,745,113 + +0.1 1,745,114 - +0.4 1,745,187 + Psyr_1552 0.22 -0.0 1,745,187 + Psyr_1552 0.22 -2.3 1,745,187 + Psyr_1552 0.22 -1.1 1,745,187 + Psyr_1552 0.22 +0.6 1,745,187 + Psyr_1552 0.22 -0.1 1,745,187 + Psyr_1552 0.22 +1.0 1,745,188 - Psyr_1552 0.23 +0.8 1,745,188 - Psyr_1552 0.23 -2.2 1,745,188 - Psyr_1552 0.23 +2.1 1,745,188 - Psyr_1552 0.23 -1.3 1,745,188 - Psyr_1552 0.23 +0.8 1,745,262 + Psyr_1552 0.41 -1.6 1,745,262 + Psyr_1552 0.41 +0.2 1,745,263 - Psyr_1552 0.41 -0.4 1,745,263 - Psyr_1552 0.41 +1.0 1,745,263 - Psyr_1552 0.41 -0.7 1,745,263 - Psyr_1552 0.41 -1.1 1,745,263 - Psyr_1552 0.41 -0.2 1,745,269 + Psyr_1552 0.43 +1.3 1,745,269 + Psyr_1552 0.43 -0.7 1,745,269 + Psyr_1552 0.43 -0.6 1,745,269 + Psyr_1552 0.43 +1.4 1,745,270 - Psyr_1552 0.43 +0.6 1,745,270 - Psyr_1552 0.43 +1.3 1,745,270 - Psyr_1552 0.43 +2.3 1,745,270 - Psyr_1552 0.43 +0.8 1,745,270 - Psyr_1552 0.43 -1.5 1,745,270 - Psyr_1552 0.43 -0.7 1,745,270 - Psyr_1552 0.43 +1.8 1,745,270 - Psyr_1552 0.43 +0.3 1,745,270 - Psyr_1552 0.43 +1.7 1,745,270 - Psyr_1552 0.43 -0.5 1,745,270 - Psyr_1552 0.43 +1.4 1,745,270 - Psyr_1552 0.43 -0.2 1,745,270 - Psyr_1552 0.43 +0.6 1,745,270 - Psyr_1552 0.43 -0.1 1,745,278 - Psyr_1552 0.45 -0.1 1,745,316 + Psyr_1552 0.55 +0.5 1,745,316 + Psyr_1552 0.55 -0.7 1,745,317 - Psyr_1552 0.55 +0.5 1,745,317 - Psyr_1552 0.55 -0.5 1,745,317 - Psyr_1552 0.55 -0.3 1,745,498 + -1.7 1,745,498 + -1.2 1,745,499 - -0.3 1,745,499 - +2.0 1,745,499 - +0.3 1,745,551 - +0.7 1,745,551 - +0.3 1,745,551 - -0.3 1,745,551 - +0.5 1,745,584 + +0.4 1,745,613 + -1.7 1,745,613 + -0.2 1,745,614 - -1.1 1,745,655 + -0.5 1,745,655 + -1.2 1,745,700 + -1.5 1,745,785 - -0.2 1,745,875 - +0.1 1,745,901 + +0.8 1,745,950 + -0.4 1,745,950 + +1.0 1,745,950 + -0.4 1,745,950 + +0.1 1,745,950 + +0.3 1,745,950 + -1.2 1,745,950 + +0.9 1,745,951 - -0.0 1,745,951 - -0.4 1,745,951 - -1.2 1,745,951 - -0.0 1,745,951 - -0.2 1,746,053 - +0.6 1,746,056 - -1.1 1,746,056 - +0.6 1,746,056 - +0.3 1,746,056 - +0.8 1,746,087 + -0.7 1,746,110 + -1.1 1,746,158 + Psyr_1553 0.18 +0.1 1,746,200 + Psyr_1553 0.30 -0.2 1,746,200 + Psyr_1553 0.30 -0.2 1,746,201 - Psyr_1553 0.30 -0.1 1,746,201 - Psyr_1553 0.30 -0.3 1,746,292 + Psyr_1553 0.57 +0.3 1,746,348 - Psyr_1553 0.74 -0.4 1,746,348 - Psyr_1553 0.74 -0.6 1,746,351 + Psyr_1553 0.75 -0.3 1,746,351 + Psyr_1553 0.75 +0.4 1,746,351 + Psyr_1553 0.75 +0.9 1,746,352 - Psyr_1553 0.75 -1.5 1,746,352 - Psyr_1553 0.75 -0.7 1,746,352 - Psyr_1553 0.75 -0.2 1,746,352 - Psyr_1553 0.75 -0.7 1,746,354 - Psyr_1553 0.76 -1.7 1,746,422 + +1.0 1,746,423 - -0.2 1,746,459 + +0.8 1,746,470 + -1.1
Or see this region's nucleotide sequence