Experiment: KB with Isosafrole 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0419 and Psyr_0420 are separated by 77 nucleotides Psyr_0420 and Psyr_0421 are separated by 115 nucleotides
Psyr_0419: Psyr_0419 - conserved hypothetical protein, at 457,693 to 458,958
_0419
Psyr_0420: Psyr_0420 - General substrate transporter:Major facilitator superfamily, at 459,036 to 460,304
_0420
Psyr_0421: Psyr_0421 - transcriptional regulator, LysR family, at 460,420 to 461,310
_0421
Position (kb) 459
460
461 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 458.122 kb on + strand, within Psyr_0419 at 458.122 kb on + strand, within Psyr_0419 at 458.122 kb on + strand, within Psyr_0419 at 458.122 kb on + strand, within Psyr_0419 at 458.122 kb on + strand, within Psyr_0419 at 458.123 kb on - strand, within Psyr_0419 at 458.123 kb on - strand, within Psyr_0419 at 458.123 kb on - strand, within Psyr_0419 at 458.150 kb on - strand, within Psyr_0419 at 458.161 kb on - strand, within Psyr_0419 at 458.276 kb on + strand, within Psyr_0419 at 458.276 kb on + strand, within Psyr_0419 at 458.277 kb on - strand, within Psyr_0419 at 458.281 kb on + strand, within Psyr_0419 at 458.281 kb on + strand, within Psyr_0419 at 458.281 kb on + strand, within Psyr_0419 at 458.281 kb on + strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.282 kb on - strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.456 kb on + strand, within Psyr_0419 at 458.457 kb on - strand, within Psyr_0419 at 458.457 kb on - strand, within Psyr_0419 at 458.503 kb on + strand, within Psyr_0419 at 458.538 kb on - strand, within Psyr_0419 at 458.538 kb on - strand, within Psyr_0419 at 458.549 kb on - strand, within Psyr_0419 at 458.549 kb on - strand, within Psyr_0419 at 458.602 kb on + strand, within Psyr_0419 at 458.602 kb on + strand, within Psyr_0419 at 458.656 kb on + strand, within Psyr_0419 at 458.656 kb on + strand, within Psyr_0419 at 458.656 kb on + strand, within Psyr_0419 at 458.657 kb on - strand, within Psyr_0419 at 458.711 kb on + strand, within Psyr_0419 at 458.747 kb on - strand, within Psyr_0419 at 458.809 kb on + strand, within Psyr_0419 at 458.809 kb on + strand, within Psyr_0419 at 458.810 kb on - strand, within Psyr_0419 at 458.810 kb on - strand, within Psyr_0419 at 458.919 kb on - strand at 458.997 kb on + strand at 458.997 kb on + strand at 458.997 kb on + strand at 458.997 kb on + strand at 458.997 kb on + strand at 458.998 kb on - strand at 458.998 kb on - strand at 458.998 kb on - strand at 458.998 kb on - strand at 459.027 kb on + strand at 459.027 kb on + strand at 459.027 kb on + strand at 459.028 kb on - strand at 459.028 kb on - strand at 459.028 kb on - strand at 459.028 kb on - strand at 459.111 kb on + strand at 459.111 kb on + strand at 459.111 kb on + strand at 459.112 kb on - strand at 459.112 kb on - strand at 459.143 kb on - strand at 459.191 kb on - strand, within Psyr_0420 at 459.193 kb on + strand, within Psyr_0420 at 459.194 kb on - strand, within Psyr_0420 at 459.194 kb on - strand, within Psyr_0420 at 459.205 kb on + strand, within Psyr_0420 at 459.205 kb on + strand, within Psyr_0420 at 459.205 kb on + strand, within Psyr_0420 at 459.206 kb on - strand, within Psyr_0420 at 459.206 kb on - strand, within Psyr_0420 at 459.399 kb on + strand, within Psyr_0420 at 459.400 kb on - strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.436 kb on + strand, within Psyr_0420 at 459.437 kb on - strand, within Psyr_0420 at 459.437 kb on - strand, within Psyr_0420 at 459.437 kb on - strand, within Psyr_0420 at 459.437 kb on - strand, within Psyr_0420 at 459.451 kb on + strand, within Psyr_0420 at 459.451 kb on + strand, within Psyr_0420 at 459.451 kb on + strand, within Psyr_0420 at 459.452 kb on - strand, within Psyr_0420 at 459.452 kb on - strand, within Psyr_0420 at 459.493 kb on + strand, within Psyr_0420 at 459.494 kb on - strand, within Psyr_0420 at 459.523 kb on + strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.524 kb on - strand, within Psyr_0420 at 459.745 kb on + strand, within Psyr_0420 at 459.745 kb on + strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.746 kb on - strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.808 kb on + strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.809 kb on - strand, within Psyr_0420 at 459.859 kb on + strand, within Psyr_0420 at 459.859 kb on + strand, within Psyr_0420 at 459.860 kb on - strand, within Psyr_0420 at 459.860 kb on - strand, within Psyr_0420 at 459.900 kb on + strand, within Psyr_0420 at 459.900 kb on + strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 459.901 kb on - strand, within Psyr_0420 at 460.144 kb on + strand, within Psyr_0420 at 460.144 kb on + strand, within Psyr_0420 at 460.144 kb on + strand, within Psyr_0420 at 460.144 kb on + strand, within Psyr_0420 at 460.145 kb on - strand, within Psyr_0420 at 460.145 kb on - strand, within Psyr_0420 at 460.145 kb on - strand, within Psyr_0420 at 460.154 kb on + strand, within Psyr_0420 at 460.154 kb on + strand, within Psyr_0420 at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.225 kb on + strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.226 kb on - strand at 460.255 kb on - strand at 460.346 kb on + strand at 460.346 kb on + strand at 460.346 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.348 kb on + strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.349 kb on - strand at 460.372 kb on + strand at 460.374 kb on + strand at 460.397 kb on + strand at 460.398 kb on - strand at 460.441 kb on + strand at 460.442 kb on - strand at 460.728 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.972 kb on + strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 460.973 kb on - strand, within Psyr_0421 at 461.020 kb on + strand, within Psyr_0421 at 461.020 kb on + strand, within Psyr_0421 at 461.021 kb on - strand, within Psyr_0421 at 461.021 kb on - strand, within Psyr_0421 at 461.031 kb on + strand, within Psyr_0421 at 461.032 kb on - strand, within Psyr_0421 at 461.035 kb on + strand, within Psyr_0421 at 461.035 kb on + strand, within Psyr_0421 at 461.035 kb on + strand, within Psyr_0421 at 461.036 kb on - strand, within Psyr_0421 at 461.303 kb on + strand at 461.303 kb on + strand at 461.303 kb on + strand at 461.304 kb on - strand at 461.304 kb on - strand at 461.304 kb on - strand at 461.304 kb on - strand at 461.304 kb on - strand at 461.304 kb on - strand at 461.304 kb on - strand at 461.304 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Isosafrole 10 mM remove 458,122 + Psyr_0419 0.34 -0.1 458,122 + Psyr_0419 0.34 +0.6 458,122 + Psyr_0419 0.34 +0.1 458,122 + Psyr_0419 0.34 +0.1 458,122 + Psyr_0419 0.34 -0.3 458,123 - Psyr_0419 0.34 +0.9 458,123 - Psyr_0419 0.34 +0.4 458,123 - Psyr_0419 0.34 +0.6 458,150 - Psyr_0419 0.36 +0.3 458,161 - Psyr_0419 0.37 -0.1 458,276 + Psyr_0419 0.46 -0.5 458,276 + Psyr_0419 0.46 +0.1 458,277 - Psyr_0419 0.46 -0.4 458,281 + Psyr_0419 0.46 -0.9 458,281 + Psyr_0419 0.46 -0.4 458,281 + Psyr_0419 0.46 -0.4 458,281 + Psyr_0419 0.46 +1.4 458,282 - Psyr_0419 0.47 -0.4 458,282 - Psyr_0419 0.47 -1.7 458,282 - Psyr_0419 0.47 +1.0 458,282 - Psyr_0419 0.47 -0.3 458,282 - Psyr_0419 0.47 -0.2 458,282 - Psyr_0419 0.47 +0.4 458,282 - Psyr_0419 0.47 +0.0 458,456 + Psyr_0419 0.60 +0.0 458,456 + Psyr_0419 0.60 +0.7 458,456 + Psyr_0419 0.60 -0.4 458,456 + Psyr_0419 0.60 -1.7 458,456 + Psyr_0419 0.60 -1.1 458,456 + Psyr_0419 0.60 -0.7 458,456 + Psyr_0419 0.60 +0.4 458,456 + Psyr_0419 0.60 +0.0 458,456 + Psyr_0419 0.60 -2.2 458,457 - Psyr_0419 0.60 -2.1 458,457 - Psyr_0419 0.60 -0.5 458,503 + Psyr_0419 0.64 +0.4 458,538 - Psyr_0419 0.67 +0.4 458,538 - Psyr_0419 0.67 +0.9 458,549 - Psyr_0419 0.68 -0.6 458,549 - Psyr_0419 0.68 -0.9 458,602 + Psyr_0419 0.72 -1.1 458,602 + Psyr_0419 0.72 +0.3 458,656 + Psyr_0419 0.76 -0.9 458,656 + Psyr_0419 0.76 -0.3 458,656 + Psyr_0419 0.76 -0.2 458,657 - Psyr_0419 0.76 +0.1 458,711 + Psyr_0419 0.80 -0.6 458,747 - Psyr_0419 0.83 -0.7 458,809 + Psyr_0419 0.88 +0.2 458,809 + Psyr_0419 0.88 -0.9 458,810 - Psyr_0419 0.88 -0.8 458,810 - Psyr_0419 0.88 +0.7 458,919 - +0.6 458,997 + +0.2 458,997 + -0.0 458,997 + +1.3 458,997 + +0.0 458,997 + +0.9 458,998 - +0.1 458,998 - +0.8 458,998 - +0.5 458,998 - +2.0 459,027 + +0.3 459,027 + -0.5 459,027 + -0.9 459,028 - -0.4 459,028 - +0.5 459,028 - -0.8 459,028 - -2.8 459,111 + -1.0 459,111 + +0.5 459,111 + +0.5 459,112 - -0.5 459,112 - -0.2 459,143 - -0.5 459,191 - Psyr_0420 0.12 -0.1 459,193 + Psyr_0420 0.12 +0.5 459,194 - Psyr_0420 0.12 +0.7 459,194 - Psyr_0420 0.12 -1.1 459,205 + Psyr_0420 0.13 +0.1 459,205 + Psyr_0420 0.13 -0.3 459,205 + Psyr_0420 0.13 -0.1 459,206 - Psyr_0420 0.13 -0.8 459,206 - Psyr_0420 0.13 +1.0 459,399 + Psyr_0420 0.29 +0.7 459,400 - Psyr_0420 0.29 -0.5 459,436 + Psyr_0420 0.32 -0.1 459,436 + Psyr_0420 0.32 -0.3 459,436 + Psyr_0420 0.32 +0.7 459,436 + Psyr_0420 0.32 -0.5 459,436 + Psyr_0420 0.32 -0.5 459,436 + Psyr_0420 0.32 +0.1 459,436 + Psyr_0420 0.32 +1.1 459,436 + Psyr_0420 0.32 -0.8 459,437 - Psyr_0420 0.32 +0.3 459,437 - Psyr_0420 0.32 +2.5 459,437 - Psyr_0420 0.32 -0.1 459,437 - Psyr_0420 0.32 -0.7 459,451 + Psyr_0420 0.33 -1.4 459,451 + Psyr_0420 0.33 -0.5 459,451 + Psyr_0420 0.33 +0.6 459,452 - Psyr_0420 0.33 -0.9 459,452 - Psyr_0420 0.33 +0.3 459,493 + Psyr_0420 0.36 +0.3 459,494 - Psyr_0420 0.36 -1.9 459,523 + Psyr_0420 0.38 +0.5 459,524 - Psyr_0420 0.38 +0.6 459,524 - Psyr_0420 0.38 +0.5 459,524 - Psyr_0420 0.38 +0.9 459,524 - Psyr_0420 0.38 -0.1 459,524 - Psyr_0420 0.38 -0.4 459,524 - Psyr_0420 0.38 -0.2 459,524 - Psyr_0420 0.38 -0.7 459,745 + Psyr_0420 0.56 +1.2 459,745 + Psyr_0420 0.56 -0.3 459,746 - Psyr_0420 0.56 -0.7 459,746 - Psyr_0420 0.56 -0.4 459,746 - Psyr_0420 0.56 -1.1 459,746 - Psyr_0420 0.56 -1.3 459,746 - Psyr_0420 0.56 -1.5 459,746 - Psyr_0420 0.56 +0.3 459,746 - Psyr_0420 0.56 +0.0 459,808 + Psyr_0420 0.61 -0.4 459,808 + Psyr_0420 0.61 +0.7 459,808 + Psyr_0420 0.61 +0.2 459,808 + Psyr_0420 0.61 +0.3 459,808 + Psyr_0420 0.61 -1.5 459,808 + Psyr_0420 0.61 -1.5 459,808 + Psyr_0420 0.61 -0.2 459,808 + Psyr_0420 0.61 -1.9 459,808 + Psyr_0420 0.61 -0.6 459,809 - Psyr_0420 0.61 +1.2 459,809 - Psyr_0420 0.61 -1.3 459,809 - Psyr_0420 0.61 -1.1 459,809 - Psyr_0420 0.61 -0.1 459,809 - Psyr_0420 0.61 +0.6 459,809 - Psyr_0420 0.61 +0.2 459,809 - Psyr_0420 0.61 -0.5 459,809 - Psyr_0420 0.61 -0.3 459,809 - Psyr_0420 0.61 -0.8 459,809 - Psyr_0420 0.61 +1.0 459,809 - Psyr_0420 0.61 +0.5 459,859 + Psyr_0420 0.65 -0.7 459,859 + Psyr_0420 0.65 +0.9 459,860 - Psyr_0420 0.65 -0.5 459,860 - Psyr_0420 0.65 +0.0 459,900 + Psyr_0420 0.68 -0.9 459,900 + Psyr_0420 0.68 -0.3 459,901 - Psyr_0420 0.68 +0.6 459,901 - Psyr_0420 0.68 +0.6 459,901 - Psyr_0420 0.68 -0.7 459,901 - Psyr_0420 0.68 -1.3 459,901 - Psyr_0420 0.68 -0.1 459,901 - Psyr_0420 0.68 +0.3 459,901 - Psyr_0420 0.68 +0.5 460,144 + Psyr_0420 0.87 -0.4 460,144 + Psyr_0420 0.87 +0.2 460,144 + Psyr_0420 0.87 -1.7 460,144 + Psyr_0420 0.87 -1.0 460,145 - Psyr_0420 0.87 -0.9 460,145 - Psyr_0420 0.87 +0.1 460,145 - Psyr_0420 0.87 +1.0 460,154 + Psyr_0420 0.88 -0.3 460,154 + Psyr_0420 0.88 +0.7 460,225 + -1.9 460,225 + -0.1 460,225 + +0.2 460,225 + -2.9 460,225 + -0.8 460,225 + +0.3 460,225 + +1.0 460,225 + +0.7 460,225 + -0.4 460,225 + -0.0 460,225 + +0.6 460,225 + -0.3 460,225 + -0.4 460,225 + +0.3 460,226 - -0.1 460,226 - +0.5 460,226 - -0.4 460,226 - +0.3 460,226 - +0.5 460,226 - +1.7 460,226 - -0.5 460,226 - -1.5 460,226 - +1.1 460,226 - -0.1 460,255 - -0.2 460,346 + -0.0 460,346 + +0.4 460,346 + +0.5 460,348 + -0.1 460,348 + +0.5 460,348 + +1.2 460,348 + -0.7 460,348 + -0.1 460,348 + +0.4 460,348 + -1.5 460,348 + +0.2 460,348 + -0.9 460,348 + -0.1 460,348 + +2.7 460,348 + +0.0 460,348 + -0.6 460,348 + +0.5 460,348 + +0.7 460,348 + -0.5 460,349 - -0.2 460,349 - +0.3 460,349 - +0.8 460,349 - -1.2 460,349 - +0.1 460,349 - +0.5 460,349 - +0.6 460,349 - -0.5 460,349 - -1.0 460,349 - -1.1 460,349 - +1.0 460,349 - -0.1 460,349 - +0.2 460,349 - -0.4 460,372 + -0.8 460,374 + -2.0 460,397 + -0.6 460,398 - +0.5 460,441 + +0.8 460,442 - +0.7 460,728 + Psyr_0421 0.35 +1.0 460,972 + Psyr_0421 0.62 +1.0 460,972 + Psyr_0421 0.62 -0.1 460,972 + Psyr_0421 0.62 -0.4 460,972 + Psyr_0421 0.62 -0.3 460,972 + Psyr_0421 0.62 +0.2 460,972 + Psyr_0421 0.62 +0.3 460,972 + Psyr_0421 0.62 -1.2 460,972 + Psyr_0421 0.62 +0.1 460,973 - Psyr_0421 0.62 +0.2 460,973 - Psyr_0421 0.62 +0.1 460,973 - Psyr_0421 0.62 +0.1 460,973 - Psyr_0421 0.62 +0.3 460,973 - Psyr_0421 0.62 -1.4 460,973 - Psyr_0421 0.62 +0.3 460,973 - Psyr_0421 0.62 +1.1 460,973 - Psyr_0421 0.62 -0.1 460,973 - Psyr_0421 0.62 +0.9 460,973 - Psyr_0421 0.62 +0.5 460,973 - Psyr_0421 0.62 -1.7 460,973 - Psyr_0421 0.62 +0.0 460,973 - Psyr_0421 0.62 +0.0 461,020 + Psyr_0421 0.67 +0.6 461,020 + Psyr_0421 0.67 -0.9 461,021 - Psyr_0421 0.67 +0.0 461,021 - Psyr_0421 0.67 -0.2 461,031 + Psyr_0421 0.69 -1.5 461,032 - Psyr_0421 0.69 -1.1 461,035 + Psyr_0421 0.69 +0.6 461,035 + Psyr_0421 0.69 -1.9 461,035 + Psyr_0421 0.69 +1.0 461,036 - Psyr_0421 0.69 -0.8 461,303 + -1.1 461,303 + -0.3 461,303 + -0.2 461,304 - -0.5 461,304 - +0.7 461,304 - +0.1 461,304 - -0.1 461,304 - +0.3 461,304 - -0.3 461,304 - -0.3 461,304 - +0.1
Or see this region's nucleotide sequence