Experiment: KB with Isosafrole 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0358 and Psyr_0359 are separated by 219 nucleotides Psyr_0359 and Psyr_0360 are separated by 197 nucleotides
Psyr_0358: Psyr_0358 - Diaminopimelate-binding protein / L-cystine-binding protein, at 383,399 to 384,196
_0358
Psyr_0359: Psyr_0359 - D-cysteine desulfhydrase, at 384,416 to 385,414
_0359
Psyr_0360: Psyr_0360 - serine O-acetyltransferase, at 385,612 to 386,565
_0360
Position (kb)
384
385
386 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 383.512 kb on + strand, within Psyr_0358 at 383.636 kb on + strand, within Psyr_0358 at 383.636 kb on + strand, within Psyr_0358 at 383.636 kb on + strand, within Psyr_0358 at 383.636 kb on + strand, within Psyr_0358 at 383.636 kb on + strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.637 kb on - strand, within Psyr_0358 at 383.674 kb on - strand, within Psyr_0358 at 383.674 kb on - strand, within Psyr_0358 at 383.674 kb on - strand, within Psyr_0358 at 383.674 kb on - strand, within Psyr_0358 at 383.721 kb on + strand, within Psyr_0358 at 383.721 kb on + strand, within Psyr_0358 at 383.721 kb on + strand, within Psyr_0358 at 383.722 kb on - strand, within Psyr_0358 at 383.722 kb on - strand, within Psyr_0358 at 383.830 kb on - strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 383.847 kb on + strand, within Psyr_0358 at 383.848 kb on - strand, within Psyr_0358 at 383.848 kb on - strand, within Psyr_0358 at 383.848 kb on - strand, within Psyr_0358 at 383.848 kb on - strand, within Psyr_0358 at 384.048 kb on + strand, within Psyr_0358 at 384.049 kb on - strand, within Psyr_0358 at 384.049 kb on - strand, within Psyr_0358 at 384.172 kb on + strand at 384.173 kb on - strand at 384.173 kb on - strand at 384.287 kb on + strand at 384.287 kb on + strand at 384.287 kb on + strand at 384.288 kb on - strand at 384.298 kb on + strand at 384.331 kb on + strand at 384.331 kb on + strand at 384.331 kb on + strand at 384.331 kb on + strand at 384.331 kb on + strand at 384.331 kb on + strand at 384.331 kb on + strand at 384.331 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.333 kb on + strand at 384.335 kb on + strand at 384.335 kb on + strand at 384.335 kb on + strand at 384.335 kb on + strand at 384.335 kb on + strand at 384.336 kb on - strand at 384.409 kb on + strand at 384.546 kb on + strand, within Psyr_0359 at 384.546 kb on + strand, within Psyr_0359 at 384.547 kb on - strand, within Psyr_0359 at 384.639 kb on + strand, within Psyr_0359 at 384.639 kb on + strand, within Psyr_0359 at 384.640 kb on - strand, within Psyr_0359 at 384.640 kb on - strand, within Psyr_0359 at 384.909 kb on + strand, within Psyr_0359 at 384.909 kb on + strand, within Psyr_0359 at 384.909 kb on + strand, within Psyr_0359 at 384.910 kb on - strand, within Psyr_0359 at 384.910 kb on - strand, within Psyr_0359 at 384.955 kb on - strand, within Psyr_0359 at 385.037 kb on + strand, within Psyr_0359 at 385.038 kb on - strand, within Psyr_0359 at 385.038 kb on - strand, within Psyr_0359 at 385.038 kb on - strand, within Psyr_0359 at 385.245 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.308 kb on + strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.309 kb on - strand, within Psyr_0359 at 385.312 kb on + strand, within Psyr_0359 at 385.312 kb on + strand, within Psyr_0359 at 385.312 kb on + strand, within Psyr_0359 at 385.434 kb on - strand at 385.434 kb on - strand at 385.450 kb on - strand at 385.451 kb on + strand at 385.451 kb on + strand at 385.451 kb on + strand at 385.452 kb on - strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.478 kb on + strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.479 kb on - strand at 385.504 kb on + strand at 385.559 kb on + strand at 385.559 kb on + strand at 385.559 kb on + strand at 385.559 kb on + strand at 385.559 kb on + strand at 385.559 kb on + strand at 385.559 kb on + strand at 385.560 kb on - strand at 385.619 kb on - strand at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.858 kb on + strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.859 kb on - strand, within Psyr_0360 at 385.924 kb on + strand, within Psyr_0360 at 386.050 kb on + strand, within Psyr_0360 at 386.050 kb on + strand, within Psyr_0360 at 386.140 kb on + strand, within Psyr_0360 at 386.140 kb on + strand, within Psyr_0360 at 386.140 kb on + strand, within Psyr_0360 at 386.140 kb on + strand, within Psyr_0360 at 386.140 kb on + strand, within Psyr_0360 at 386.140 kb on + strand, within Psyr_0360 at 386.140 kb on + strand, within Psyr_0360 at 386.141 kb on - strand, within Psyr_0360 at 386.141 kb on - strand, within Psyr_0360 at 386.314 kb on + strand, within Psyr_0360 at 386.314 kb on + strand, within Psyr_0360 at 386.315 kb on - strand, within Psyr_0360 at 386.315 kb on - strand, within Psyr_0360
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Isosafrole 10 mM remove 383,512 + Psyr_0358 0.14 +1.2 383,636 + Psyr_0358 0.30 +1.1 383,636 + Psyr_0358 0.30 +0.7 383,636 + Psyr_0358 0.30 -0.0 383,636 + Psyr_0358 0.30 +0.6 383,636 + Psyr_0358 0.30 -0.4 383,637 - Psyr_0358 0.30 -0.3 383,637 - Psyr_0358 0.30 +0.1 383,637 - Psyr_0358 0.30 -1.6 383,637 - Psyr_0358 0.30 -1.4 383,674 - Psyr_0358 0.34 +0.1 383,674 - Psyr_0358 0.34 +0.1 383,674 - Psyr_0358 0.34 -1.1 383,674 - Psyr_0358 0.34 -0.4 383,721 + Psyr_0358 0.40 -0.3 383,721 + Psyr_0358 0.40 +0.5 383,721 + Psyr_0358 0.40 -0.8 383,722 - Psyr_0358 0.40 -1.3 383,722 - Psyr_0358 0.40 -0.1 383,830 - Psyr_0358 0.54 +0.3 383,847 + Psyr_0358 0.56 -1.5 383,847 + Psyr_0358 0.56 +0.3 383,847 + Psyr_0358 0.56 -1.9 383,847 + Psyr_0358 0.56 +0.0 383,847 + Psyr_0358 0.56 -0.7 383,848 - Psyr_0358 0.56 +0.4 383,848 - Psyr_0358 0.56 -0.0 383,848 - Psyr_0358 0.56 +0.5 383,848 - Psyr_0358 0.56 -0.1 384,048 + Psyr_0358 0.81 -0.1 384,049 - Psyr_0358 0.81 -0.0 384,049 - Psyr_0358 0.81 -1.3 384,172 + -0.0 384,173 - +0.7 384,173 - +0.8 384,287 + -0.5 384,287 + -0.3 384,287 + -0.6 384,288 - +0.5 384,298 + -0.0 384,331 + +0.0 384,331 + +0.6 384,331 + +0.1 384,331 + +2.3 384,331 + -0.8 384,331 + -1.0 384,331 + +0.4 384,331 + +0.8 384,333 + +0.9 384,333 + -0.2 384,333 + +0.7 384,333 + -0.0 384,333 + -0.0 384,335 + +0.8 384,335 + -0.3 384,335 + -0.5 384,335 + +1.0 384,335 + +0.2 384,336 - -1.2 384,409 + +0.5 384,546 + Psyr_0359 0.13 +1.3 384,546 + Psyr_0359 0.13 +1.5 384,547 - Psyr_0359 0.13 +1.0 384,639 + Psyr_0359 0.22 +1.3 384,639 + Psyr_0359 0.22 +1.5 384,640 - Psyr_0359 0.22 +0.5 384,640 - Psyr_0359 0.22 +1.0 384,909 + Psyr_0359 0.49 -0.5 384,909 + Psyr_0359 0.49 -0.1 384,909 + Psyr_0359 0.49 -0.9 384,910 - Psyr_0359 0.49 -0.1 384,910 - Psyr_0359 0.49 +1.3 384,955 - Psyr_0359 0.54 -2.0 385,037 + Psyr_0359 0.62 -1.0 385,038 - Psyr_0359 0.62 -0.2 385,038 - Psyr_0359 0.62 +0.3 385,038 - Psyr_0359 0.62 -0.0 385,245 + Psyr_0359 0.83 -0.0 385,308 + Psyr_0359 0.89 -0.6 385,308 + Psyr_0359 0.89 +1.3 385,308 + Psyr_0359 0.89 -1.5 385,308 + Psyr_0359 0.89 +0.7 385,308 + Psyr_0359 0.89 -0.9 385,308 + Psyr_0359 0.89 +0.5 385,308 + Psyr_0359 0.89 -0.3 385,308 + Psyr_0359 0.89 -1.7 385,308 + Psyr_0359 0.89 +0.2 385,308 + Psyr_0359 0.89 -0.1 385,308 + Psyr_0359 0.89 +0.3 385,308 + Psyr_0359 0.89 +0.5 385,308 + Psyr_0359 0.89 +0.2 385,308 + Psyr_0359 0.89 -1.8 385,308 + Psyr_0359 0.89 +0.4 385,308 + Psyr_0359 0.89 -1.7 385,308 + Psyr_0359 0.89 +1.8 385,308 + Psyr_0359 0.89 -1.0 385,308 + Psyr_0359 0.89 -1.9 385,309 - Psyr_0359 0.89 +0.3 385,309 - Psyr_0359 0.89 +0.7 385,309 - Psyr_0359 0.89 -0.2 385,309 - Psyr_0359 0.89 +0.0 385,309 - Psyr_0359 0.89 +0.5 385,309 - Psyr_0359 0.89 -0.2 385,309 - Psyr_0359 0.89 -0.4 385,309 - Psyr_0359 0.89 -0.5 385,309 - Psyr_0359 0.89 +0.2 385,309 - Psyr_0359 0.89 +0.5 385,309 - Psyr_0359 0.89 -0.3 385,312 + Psyr_0359 0.90 -0.3 385,312 + Psyr_0359 0.90 +0.1 385,312 + Psyr_0359 0.90 +0.0 385,434 - +0.5 385,434 - -2.0 385,450 - -0.5 385,451 + +1.0 385,451 + -0.3 385,451 + +0.1 385,452 - +0.2 385,478 + -0.5 385,478 + -0.6 385,478 + -1.5 385,478 + +0.6 385,478 + +0.4 385,478 + -0.6 385,478 + -0.9 385,478 + -0.1 385,478 + +0.0 385,479 - -0.6 385,479 - -0.9 385,479 - -2.2 385,479 - -0.4 385,479 - +0.2 385,479 - -0.5 385,479 - -0.1 385,479 - +2.0 385,504 + -0.1 385,559 + +0.3 385,559 + +0.3 385,559 + +0.2 385,559 + +0.9 385,559 + +0.7 385,559 + -1.1 385,559 + +1.0 385,560 - -0.3 385,619 - +0.2 385,858 + Psyr_0360 0.26 -0.3 385,858 + Psyr_0360 0.26 -0.2 385,858 + Psyr_0360 0.26 -1.5 385,858 + Psyr_0360 0.26 -1.3 385,858 + Psyr_0360 0.26 +0.5 385,858 + Psyr_0360 0.26 +0.7 385,858 + Psyr_0360 0.26 -0.2 385,858 + Psyr_0360 0.26 -0.1 385,858 + Psyr_0360 0.26 +2.5 385,858 + Psyr_0360 0.26 -0.4 385,858 + Psyr_0360 0.26 -0.0 385,858 + Psyr_0360 0.26 +0.6 385,858 + Psyr_0360 0.26 -0.5 385,858 + Psyr_0360 0.26 +0.5 385,859 - Psyr_0360 0.26 +0.6 385,859 - Psyr_0360 0.26 -2.1 385,859 - Psyr_0360 0.26 +0.0 385,859 - Psyr_0360 0.26 -0.0 385,859 - Psyr_0360 0.26 +0.3 385,859 - Psyr_0360 0.26 +0.3 385,859 - Psyr_0360 0.26 -1.0 385,859 - Psyr_0360 0.26 -0.5 385,859 - Psyr_0360 0.26 +0.0 385,924 + Psyr_0360 0.33 -1.3 386,050 + Psyr_0360 0.46 +0.9 386,050 + Psyr_0360 0.46 +0.5 386,140 + Psyr_0360 0.55 -0.6 386,140 + Psyr_0360 0.55 -0.4 386,140 + Psyr_0360 0.55 -2.9 386,140 + Psyr_0360 0.55 +0.6 386,140 + Psyr_0360 0.55 -0.4 386,140 + Psyr_0360 0.55 -2.4 386,140 + Psyr_0360 0.55 +0.9 386,141 - Psyr_0360 0.55 -0.4 386,141 - Psyr_0360 0.55 -1.1 386,314 + Psyr_0360 0.74 -1.8 386,314 + Psyr_0360 0.74 -0.3 386,315 - Psyr_0360 0.74 -2.1 386,315 - Psyr_0360 0.74 +0.2
Or see this region's nucleotide sequence