Experiment: KB with Estragole 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2940 and Psyr_2941 are separated by 496 nucleotides Psyr_2941 and Psyr_2942 overlap by 4 nucleotides Psyr_2942 and Psyr_2943 overlap by 4 nucleotides
Psyr_2940: Psyr_2940 - PAS, at 3,527,685 to 3,531,746
_2940
Psyr_2941: Psyr_2941 - amino acid ABC transporter substrate-binding protein, PAAT family, at 3,532,243 to 3,533,115
_2941
Psyr_2942: Psyr_2942 - amino acid ABC transporter ATP-binding protein, PAAT family, at 3,533,112 to 3,533,873
_2942
Psyr_2943: Psyr_2943 - amino acid ABC transporter membrane protein 2, PAAT family, at 3,533,870 to 3,534,529
_2943
Position (kb)
3532
3533
3534 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3531.252 kb on - strand, within Psyr_2940 at 3531.391 kb on - strand at 3531.509 kb on + strand at 3531.510 kb on - strand at 3531.510 kb on - strand at 3531.565 kb on + strand at 3531.565 kb on + strand at 3531.585 kb on + strand at 3531.585 kb on + strand at 3531.585 kb on + strand at 3531.585 kb on + strand at 3531.586 kb on - strand at 3531.586 kb on - strand at 3531.586 kb on - strand at 3531.586 kb on - strand at 3531.631 kb on - strand at 3531.837 kb on + strand at 3531.838 kb on - strand at 3531.956 kb on + strand at 3531.957 kb on - strand at 3531.963 kb on + strand at 3531.963 kb on + strand at 3532.042 kb on - strand at 3532.042 kb on - strand at 3532.042 kb on - strand at 3532.042 kb on - strand at 3532.046 kb on - strand at 3532.074 kb on + strand at 3532.142 kb on + strand at 3532.143 kb on - strand at 3532.312 kb on + strand at 3532.312 kb on + strand at 3532.312 kb on + strand at 3532.312 kb on + strand at 3532.312 kb on + strand at 3532.312 kb on + strand at 3532.312 kb on + strand at 3532.313 kb on - strand at 3532.313 kb on - strand at 3532.313 kb on - strand at 3532.313 kb on - strand at 3532.316 kb on + strand at 3532.316 kb on + strand at 3532.316 kb on + strand at 3532.316 kb on + strand at 3532.316 kb on + strand at 3532.316 kb on + strand at 3532.316 kb on + strand at 3532.317 kb on - strand at 3532.317 kb on - strand at 3532.317 kb on - strand at 3532.325 kb on + strand at 3532.326 kb on - strand at 3532.340 kb on + strand, within Psyr_2941 at 3532.341 kb on - strand, within Psyr_2941 at 3532.341 kb on - strand, within Psyr_2941 at 3532.512 kb on - strand, within Psyr_2941 at 3532.609 kb on - strand, within Psyr_2941 at 3532.784 kb on - strand, within Psyr_2941 at 3532.784 kb on - strand, within Psyr_2941 at 3532.784 kb on - strand, within Psyr_2941 at 3532.784 kb on - strand, within Psyr_2941 at 3532.784 kb on - strand, within Psyr_2941 at 3532.808 kb on - strand, within Psyr_2941 at 3532.908 kb on - strand, within Psyr_2941 at 3532.997 kb on + strand, within Psyr_2941 at 3533.089 kb on + strand at 3533.093 kb on - strand at 3533.093 kb on - strand at 3533.093 kb on - strand at 3533.147 kb on - strand at 3533.586 kb on + strand, within Psyr_2942 at 3533.611 kb on + strand, within Psyr_2942 at 3533.611 kb on + strand, within Psyr_2942 at 3533.611 kb on + strand, within Psyr_2942 at 3533.612 kb on - strand, within Psyr_2942 at 3533.612 kb on - strand, within Psyr_2942 at 3533.612 kb on - strand, within Psyr_2942 at 3533.932 kb on + strand at 3533.958 kb on + strand, within Psyr_2943 at 3533.959 kb on - strand, within Psyr_2943 at 3533.977 kb on - strand, within Psyr_2943 at 3534.067 kb on - strand, within Psyr_2943 at 3534.067 kb on - strand, within Psyr_2943 at 3534.067 kb on - strand, within Psyr_2943 at 3534.067 kb on - strand, within Psyr_2943 at 3534.114 kb on - strand, within Psyr_2943
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Estragole 10 mM remove 3,531,252 - Psyr_2940 0.88 +0.5 3,531,391 - +1.0 3,531,509 + -0.2 3,531,510 - -0.4 3,531,510 - +0.1 3,531,565 + -0.3 3,531,565 + +1.4 3,531,585 + +0.4 3,531,585 + -1.5 3,531,585 + +0.1 3,531,585 + -0.4 3,531,586 - -0.2 3,531,586 - +0.0 3,531,586 - +0.4 3,531,586 - +0.8 3,531,631 - +0.1 3,531,837 + -0.1 3,531,838 - -1.3 3,531,956 + +0.1 3,531,957 - +0.2 3,531,963 + -0.5 3,531,963 + +1.5 3,532,042 - +0.1 3,532,042 - -0.0 3,532,042 - +0.4 3,532,042 - +0.7 3,532,046 - +1.1 3,532,074 + +0.9 3,532,142 + +0.7 3,532,143 - -0.5 3,532,312 + -0.9 3,532,312 + +3.4 3,532,312 + +1.6 3,532,312 + -0.4 3,532,312 + -0.2 3,532,312 + +2.3 3,532,312 + -1.5 3,532,313 - +0.4 3,532,313 - +0.2 3,532,313 - -0.2 3,532,313 - +0.1 3,532,316 + +1.6 3,532,316 + -0.6 3,532,316 + +0.3 3,532,316 + +0.8 3,532,316 + -0.1 3,532,316 + -1.6 3,532,316 + +0.9 3,532,317 - -0.0 3,532,317 - +1.4 3,532,317 - +0.7 3,532,325 + +0.7 3,532,326 - +0.3 3,532,340 + Psyr_2941 0.11 -0.7 3,532,341 - Psyr_2941 0.11 -1.1 3,532,341 - Psyr_2941 0.11 -0.6 3,532,512 - Psyr_2941 0.31 -0.2 3,532,609 - Psyr_2941 0.42 +0.0 3,532,784 - Psyr_2941 0.62 -0.8 3,532,784 - Psyr_2941 0.62 -1.3 3,532,784 - Psyr_2941 0.62 +1.5 3,532,784 - Psyr_2941 0.62 +1.1 3,532,784 - Psyr_2941 0.62 -0.2 3,532,808 - Psyr_2941 0.65 -0.5 3,532,908 - Psyr_2941 0.76 -0.5 3,532,997 + Psyr_2941 0.86 -0.1 3,533,089 + +0.4 3,533,093 - +0.1 3,533,093 - +0.7 3,533,093 - +0.3 3,533,147 - -1.3 3,533,586 + Psyr_2942 0.62 +0.2 3,533,611 + Psyr_2942 0.65 +0.1 3,533,611 + Psyr_2942 0.65 -0.2 3,533,611 + Psyr_2942 0.65 -0.8 3,533,612 - Psyr_2942 0.66 -0.3 3,533,612 - Psyr_2942 0.66 +0.7 3,533,612 - Psyr_2942 0.66 +1.1 3,533,932 + +0.1 3,533,958 + Psyr_2943 0.13 -1.1 3,533,959 - Psyr_2943 0.13 +0.6 3,533,977 - Psyr_2943 0.16 +0.0 3,534,067 - Psyr_2943 0.30 -0.2 3,534,067 - Psyr_2943 0.30 -0.6 3,534,067 - Psyr_2943 0.30 +0.7 3,534,067 - Psyr_2943 0.30 -0.5 3,534,114 - Psyr_2943 0.37 -1.6
Or see this region's nucleotide sequence