Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22230 and BWI76_RS22235 are separated by 133 nucleotides BWI76_RS22235 and BWI76_RS22240 are separated by 68 nucleotides BWI76_RS22240 and BWI76_RS22245 overlap by 8 nucleotides BWI76_RS22245 and BWI76_RS22250 overlap by 38 nucleotides
BWI76_RS22230: BWI76_RS22230 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140) (from data) , at 4,634,055 to 4,634,834
_RS22230
BWI76_RS22235: BWI76_RS22235 - transcriptional regulator GutM, at 4,634,968 to 4,635,327
_RS22235
BWI76_RS22240: BWI76_RS22240 - transcriptional regulator, at 4,635,396 to 4,636,169
_RS22240
BWI76_RS22245: BWI76_RS22245 - arabinose 5-phosphate isomerase GutQ, at 4,636,162 to 4,637,127
_RS22245
BWI76_RS22250: BWI76_RS22250 - nitric oxide reductase transcription regulator, at 4,637,090 to 4,638,634
_RS22250
Position (kb)
4635
4636
4637 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4634.454 kb on - strand, within BWI76_RS22230 at 4634.508 kb on + strand, within BWI76_RS22230 at 4634.581 kb on + strand, within BWI76_RS22230 at 4634.664 kb on - strand, within BWI76_RS22230 at 4634.679 kb on - strand, within BWI76_RS22230 at 4635.182 kb on - strand, within BWI76_RS22235 at 4635.467 kb on - strand at 4635.495 kb on - strand, within BWI76_RS22240 at 4635.583 kb on - strand, within BWI76_RS22240 at 4635.705 kb on + strand, within BWI76_RS22240 at 4635.964 kb on - strand, within BWI76_RS22240 at 4636.176 kb on + strand at 4636.196 kb on + strand at 4636.356 kb on - strand, within BWI76_RS22245 at 4636.472 kb on - strand, within BWI76_RS22245 at 4636.513 kb on - strand, within BWI76_RS22245 at 4636.622 kb on + strand, within BWI76_RS22245 at 4636.623 kb on + strand, within BWI76_RS22245 at 4636.835 kb on - strand, within BWI76_RS22245 at 4636.847 kb on + strand, within BWI76_RS22245 at 4637.022 kb on - strand, within BWI76_RS22245 at 4637.163 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 4,634,454 - BWI76_RS22230 0.51 +1.3 4,634,508 + BWI76_RS22230 0.58 -0.8 4,634,581 + BWI76_RS22230 0.67 +0.7 4,634,664 - BWI76_RS22230 0.78 -0.1 4,634,679 - BWI76_RS22230 0.80 -0.8 4,635,182 - BWI76_RS22235 0.59 -0.1 4,635,467 - -1.5 4,635,495 - BWI76_RS22240 0.13 -5.0 4,635,583 - BWI76_RS22240 0.24 -5.0 4,635,705 + BWI76_RS22240 0.40 -2.6 4,635,964 - BWI76_RS22240 0.73 -1.8 4,636,176 + -0.5 4,636,196 + -0.6 4,636,356 - BWI76_RS22245 0.20 -0.8 4,636,472 - BWI76_RS22245 0.32 +0.4 4,636,513 - BWI76_RS22245 0.36 -2.7 4,636,622 + BWI76_RS22245 0.48 -0.1 4,636,623 + BWI76_RS22245 0.48 +0.9 4,636,835 - BWI76_RS22245 0.70 +0.2 4,636,847 + BWI76_RS22245 0.71 -0.9 4,637,022 - BWI76_RS22245 0.89 +1.4 4,637,163 - +0.8
Or see this region's nucleotide sequence