Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20180

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20170 and BWI76_RS20175 overlap by 4 nucleotidesBWI76_RS20175 and BWI76_RS20180 are separated by 2 nucleotidesBWI76_RS20180 and BWI76_RS20185 are separated by 98 nucleotidesBWI76_RS20185 and BWI76_RS20190 are separated by 15 nucleotides BWI76_RS20170: BWI76_RS20170 - NADH-quinone oxidoreductase subunit F, at 4,227,130 to 4,228,467 _RS20170 BWI76_RS20175: BWI76_RS20175 - NADH-quinone oxidoreductase subunit E, at 4,228,464 to 4,228,964 _RS20175 BWI76_RS20180: BWI76_RS20180 - NADH-quinone oxidoreductase subunit C/D, at 4,228,967 to 4,230,763 _RS20180 BWI76_RS20185: BWI76_RS20185 - NADH-quinone oxidoreductase subunit B, at 4,230,862 to 4,231,536 _RS20185 BWI76_RS20190: BWI76_RS20190 - NADH-quinone oxidoreductase subunit A, at 4,231,552 to 4,231,998 _RS20190 Position (kb) 4228 4229 4230 4231Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4228.008 kb on - strand, within BWI76_RS20170at 4228.295 kb on - strand, within BWI76_RS20170at 4228.438 kb on + strandat 4228.521 kb on - strand, within BWI76_RS20175at 4229.509 kb on - strand, within BWI76_RS20180at 4229.533 kb on + strand, within BWI76_RS20180at 4229.573 kb on - strand, within BWI76_RS20180at 4229.753 kb on + strand, within BWI76_RS20180at 4230.095 kb on + strand, within BWI76_RS20180at 4230.096 kb on - strand, within BWI76_RS20180at 4230.263 kb on - strand, within BWI76_RS20180at 4230.307 kb on + strand, within BWI76_RS20180at 4230.870 kb on + strandat 4231.087 kb on + strand, within BWI76_RS20185at 4231.302 kb on - strandat 4231.557 kb on + strandat 4231.565 kb on - strandat 4231.591 kb on - strandat 4231.657 kb on - strand, within BWI76_RS20190

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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4,228,008 - BWI76_RS20170 0.66 -5.3
4,228,295 - BWI76_RS20170 0.87 -5.4
4,228,438 + -4.2
4,228,521 - BWI76_RS20175 0.11 -1.7
4,229,509 - BWI76_RS20180 0.30 -4.5
4,229,533 + BWI76_RS20180 0.31 -3.1
4,229,573 - BWI76_RS20180 0.34 -1.3
4,229,753 + BWI76_RS20180 0.44 -3.2
4,230,095 + BWI76_RS20180 0.63 -2.5
4,230,096 - BWI76_RS20180 0.63 -4.4
4,230,263 - BWI76_RS20180 0.72 +0.7
4,230,307 + BWI76_RS20180 0.75 -3.4
4,230,870 + -0.9
4,231,087 + BWI76_RS20185 0.33 -1.5
4,231,302 - -0.9
4,231,557 + -2.3
4,231,565 - -3.1
4,231,591 - -0.4
4,231,657 - BWI76_RS20190 0.23 -3.0

Or see this region's nucleotide sequence