Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16525

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16520 and BWI76_RS16525 are separated by 298 nucleotidesBWI76_RS16525 and BWI76_RS16530 are separated by 31 nucleotidesBWI76_RS16530 and BWI76_RS16535 are separated by 230 nucleotides BWI76_RS16520: BWI76_RS16520 - Bcr/CflA family multidrug efflux MFS transporter, at 3,424,569 to 3,425,753 _RS16520 BWI76_RS16525: BWI76_RS16525 - cyclopropane-fatty-acyl-phospholipid synthase, at 3,426,052 to 3,427,203 _RS16525 BWI76_RS16530: BWI76_RS16530 - riboflavin synthase subunit alpha, at 3,427,235 to 3,427,876 _RS16530 BWI76_RS16535: BWI76_RS16535 - MATE family efflux transporter, at 3,428,107 to 3,429,480 _RS16535 Position (kb) 3426 3427 3428Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3425.116 kb on - strand, within BWI76_RS16520at 3425.209 kb on - strand, within BWI76_RS16520at 3425.305 kb on - strand, within BWI76_RS16520at 3425.341 kb on - strand, within BWI76_RS16520at 3425.439 kb on + strand, within BWI76_RS16520at 3425.672 kb on + strandat 3425.711 kb on + strandat 3425.711 kb on + strandat 3425.747 kb on + strandat 3425.937 kb on + strandat 3426.013 kb on + strandat 3426.013 kb on + strandat 3426.214 kb on + strand, within BWI76_RS16525at 3426.658 kb on - strand, within BWI76_RS16525at 3426.928 kb on - strand, within BWI76_RS16525at 3427.025 kb on + strand, within BWI76_RS16525at 3427.174 kb on + strandat 3427.186 kb on - strandat 3427.999 kb on + strandat 3428.019 kb on - strandat 3428.091 kb on + strandat 3428.091 kb on + strandat 3428.148 kb on + strandat 3428.176 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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3,425,116 - BWI76_RS16520 0.46 -3.0
3,425,209 - BWI76_RS16520 0.54 -1.6
3,425,305 - BWI76_RS16520 0.62 -2.6
3,425,341 - BWI76_RS16520 0.65 -3.0
3,425,439 + BWI76_RS16520 0.73 -3.3
3,425,672 + -5.0
3,425,711 + -1.6
3,425,711 + -1.2
3,425,747 + -0.3
3,425,937 + -1.7
3,426,013 + +0.3
3,426,013 + -3.3
3,426,214 + BWI76_RS16525 0.14 -0.3
3,426,658 - BWI76_RS16525 0.53 -3.3
3,426,928 - BWI76_RS16525 0.76 -2.3
3,427,025 + BWI76_RS16525 0.84 -2.7
3,427,174 + +0.1
3,427,186 - -0.0
3,427,999 + -0.8
3,428,019 - +0.7
3,428,091 + +0.3
3,428,091 + +0.7
3,428,148 + +0.1
3,428,176 + -1.3

Or see this region's nucleotide sequence