Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS08980

Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS08975 and BWI76_RS08980 are separated by 376 nucleotidesBWI76_RS08980 and BWI76_RS08985 are separated by 221 nucleotides BWI76_RS08975: BWI76_RS08975 - amino acid ABC transporter substrate-binding protein, at 1,891,396 to 1,892,142 _RS08975 BWI76_RS08980: BWI76_RS08980 - DNA starvation/stationary phase protection protein, at 1,892,519 to 1,893,022 _RS08980 BWI76_RS08985: BWI76_RS08985 - threonine/homoserine exporter RhtA, at 1,893,244 to 1,894,131 _RS08985 Position (kb) 1892 1893 1894Strain fitness (log2 ratio) -3 -2 -1 0 1at 1891.553 kb on + strand, within BWI76_RS08975at 1891.944 kb on + strand, within BWI76_RS08975at 1891.981 kb on + strand, within BWI76_RS08975at 1891.994 kb on - strand, within BWI76_RS08975at 1892.126 kb on - strandat 1892.145 kb on - strandat 1892.167 kb on + strandat 1892.167 kb on + strandat 1892.449 kb on + strandat 1892.457 kb on - strandat 1892.524 kb on - strandat 1892.683 kb on - strand, within BWI76_RS08980at 1893.108 kb on + strandat 1893.299 kb on - strandat 1893.461 kb on + strand, within BWI76_RS08985at 1893.469 kb on - strand, within BWI76_RS08985at 1893.586 kb on + strand, within BWI76_RS08985at 1893.950 kb on + strand, within BWI76_RS08985

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C)
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1,891,553 + BWI76_RS08975 0.21 -0.5
1,891,944 + BWI76_RS08975 0.73 -1.7
1,891,981 + BWI76_RS08975 0.78 -1.2
1,891,994 - BWI76_RS08975 0.80 -1.7
1,892,126 - -0.3
1,892,145 - -0.3
1,892,167 + -0.3
1,892,167 + +1.4
1,892,449 + -3.3
1,892,457 - -0.4
1,892,524 - +1.0
1,892,683 - BWI76_RS08980 0.33 +0.4
1,893,108 + +0.7
1,893,299 - +0.2
1,893,461 + BWI76_RS08985 0.24 +0.0
1,893,469 - BWI76_RS08985 0.25 +0.3
1,893,586 + BWI76_RS08985 0.39 -0.3
1,893,950 + BWI76_RS08985 0.80 +1.1

Or see this region's nucleotide sequence