Experiment: 2-Deoxyadenosine 5-monophosphate 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00070 and BWI76_RS00075 are separated by 50 nucleotides BWI76_RS00075 and BWI76_RS00080 overlap by 4 nucleotides BWI76_RS00080 and BWI76_RS00085 overlap by 4 nucleotides BWI76_RS00085 and BWI76_RS00090 are separated by 169 nucleotides
BWI76_RS00070: BWI76_RS00070 - MFS transporter, at 13,479 to 14,969
_RS00070
BWI76_RS00075: BWI76_RS00075 - N-acetylmannosamine-6-phosphate 2-epimerase, at 15,020 to 15,709
_RS00075
BWI76_RS00080: BWI76_RS00080 - N-acetylmannosamine kinase, at 15,706 to 16,581
_RS00080
BWI76_RS00085: BWI76_RS00085 - hypothetical protein, at 16,578 to 17,042
_RS00085
BWI76_RS00090: BWI76_RS00090 - multidrug transporter subunit MdtL, at 17,212 to 18,393
_RS00090
Position (kb)
15
16
17 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 14.759 kb on + strand, within BWI76_RS00070 at 14.884 kb on + strand at 14.922 kb on - strand at 14.925 kb on - strand at 14.925 kb on - strand at 15.018 kb on - strand at 15.089 kb on + strand, within BWI76_RS00075 at 15.320 kb on - strand, within BWI76_RS00075 at 15.361 kb on + strand, within BWI76_RS00075 at 16.009 kb on + strand, within BWI76_RS00080 at 16.225 kb on + strand, within BWI76_RS00080 at 16.451 kb on + strand, within BWI76_RS00080 at 16.461 kb on + strand, within BWI76_RS00080 at 16.469 kb on - strand, within BWI76_RS00080 at 16.514 kb on - strand at 16.529 kb on - strand at 16.601 kb on - strand at 16.609 kb on + strand at 16.616 kb on + strand at 16.631 kb on + strand, within BWI76_RS00085 at 16.631 kb on + strand, within BWI76_RS00085 at 16.932 kb on - strand, within BWI76_RS00085 at 17.020 kb on - strand at 17.088 kb on - strand at 17.176 kb on + strand at 17.176 kb on + strand at 17.176 kb on + strand at 17.176 kb on + strand at 17.184 kb on - strand at 17.184 kb on - strand at 17.214 kb on - strand at 17.244 kb on + strand at 17.252 kb on - strand at 17.252 kb on - strand at 17.498 kb on - strand, within BWI76_RS00090 at 17.539 kb on + strand, within BWI76_RS00090 at 17.546 kb on + strand, within BWI76_RS00090
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Deoxyadenosine 5-monophosphate 5 mM (C) remove 14,759 + BWI76_RS00070 0.86 -1.7 14,884 + -0.2 14,922 - +2.2 14,925 - -0.1 14,925 - +1.2 15,018 - +1.3 15,089 + BWI76_RS00075 0.10 +1.0 15,320 - BWI76_RS00075 0.43 +0.4 15,361 + BWI76_RS00075 0.49 -1.2 16,009 + BWI76_RS00080 0.35 +1.4 16,225 + BWI76_RS00080 0.59 +1.9 16,451 + BWI76_RS00080 0.85 +0.1 16,461 + BWI76_RS00080 0.86 -0.2 16,469 - BWI76_RS00080 0.87 -3.2 16,514 - -0.2 16,529 - +0.7 16,601 - +1.4 16,609 + -1.2 16,616 + +1.0 16,631 + BWI76_RS00085 0.11 +0.3 16,631 + BWI76_RS00085 0.11 -0.2 16,932 - BWI76_RS00085 0.76 +0.4 17,020 - -1.5 17,088 - +1.2 17,176 + -0.4 17,176 + +0.0 17,176 + +0.9 17,176 + +3.9 17,184 - -1.0 17,184 - +0.8 17,214 - -0.0 17,244 + -2.5 17,252 - -0.7 17,252 - +0.9 17,498 - BWI76_RS00090 0.24 -2.4 17,539 + BWI76_RS00090 0.28 -0.3 17,546 + BWI76_RS00090 0.28 +2.4
Or see this region's nucleotide sequence