Experiment: biofilm; attached cells from 96 well plate assay in R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE53_003965 and ABIE53_003966 are separated by 4 nucleotides ABIE53_003966 and ABIE53_003967 overlap by 42 nucleotides ABIE53_003967 and ABIE53_003968 are separated by 24 nucleotides ABIE53_003968 and ABIE53_003969 are separated by 3 nucleotides
ABIE53_003965: ABIE53_003965 - LysR family glycine cleavage system transcriptional activator, at 117,653 to 118,618
_003965
ABIE53_003966: ABIE53_003966 - long-chain acyl-CoA synthetase, at 118,623 to 120,251
_003966
ABIE53_003967: ABIE53_003967 - xanthine/CO dehydrogenase XdhC/CoxF family maturation factor, at 120,210 to 120,395
_003967
ABIE53_003968: ABIE53_003968 - 2-iminobutanoate/2-iminopropanoate deaminase, at 120,420 to 120,797
_003968
ABIE53_003969: ABIE53_003969 - MFS family permease, at 120,801 to 122,204
_003969
Position (kb) 118
119
120
121 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 117.662 kb on + strand at 117.662 kb on + strand at 117.662 kb on + strand at 117.663 kb on - strand at 117.663 kb on - strand at 117.663 kb on - strand at 117.962 kb on + strand, within ABIE53_003965 at 117.963 kb on - strand, within ABIE53_003965 at 118.073 kb on + strand, within ABIE53_003965 at 118.074 kb on - strand, within ABIE53_003965 at 118.244 kb on + strand, within ABIE53_003965 at 118.244 kb on + strand, within ABIE53_003965 at 118.930 kb on + strand, within ABIE53_003966 at 119.155 kb on + strand, within ABIE53_003966 at 119.155 kb on + strand, within ABIE53_003966 at 119.241 kb on + strand, within ABIE53_003966 at 119.242 kb on - strand, within ABIE53_003966 at 119.325 kb on + strand, within ABIE53_003966 at 119.338 kb on + strand, within ABIE53_003966 at 119.339 kb on - strand, within ABIE53_003966 at 119.378 kb on - strand, within ABIE53_003966 at 119.378 kb on - strand, within ABIE53_003966 at 119.378 kb on - strand, within ABIE53_003966 at 119.438 kb on + strand, within ABIE53_003966 at 119.610 kb on + strand, within ABIE53_003966 at 119.611 kb on - strand, within ABIE53_003966 at 119.611 kb on - strand, within ABIE53_003966 at 119.941 kb on + strand, within ABIE53_003966 at 119.941 kb on + strand, within ABIE53_003966 at 119.942 kb on - strand, within ABIE53_003966 at 120.010 kb on - strand, within ABIE53_003966 at 120.010 kb on - strand, within ABIE53_003966 at 120.010 kb on - strand, within ABIE53_003966 at 120.010 kb on - strand, within ABIE53_003966 at 120.123 kb on - strand at 120.123 kb on - strand at 120.126 kb on + strand at 120.126 kb on + strand at 120.249 kb on + strand at 120.249 kb on + strand at 120.250 kb on - strand at 120.250 kb on - strand at 120.250 kb on - strand at 120.250 kb on - strand at 120.327 kb on + strand, within ABIE53_003967 at 120.407 kb on + strand at 120.408 kb on - strand at 120.521 kb on - strand, within ABIE53_003968 at 120.571 kb on + strand, within ABIE53_003968 at 120.571 kb on + strand, within ABIE53_003968 at 120.572 kb on - strand, within ABIE53_003968 at 120.752 kb on - strand, within ABIE53_003968 at 120.816 kb on + strand at 120.872 kb on - strand at 120.872 kb on - strand at 120.872 kb on - strand at 120.872 kb on - strand at 121.046 kb on + strand, within ABIE53_003969 at 121.047 kb on - strand, within ABIE53_003969 at 121.047 kb on - strand, within ABIE53_003969 at 121.047 kb on - strand, within ABIE53_003969 at 121.047 kb on - strand, within ABIE53_003969 at 121.047 kb on - strand, within ABIE53_003969 at 121.048 kb on + strand, within ABIE53_003969 at 121.048 kb on + strand, within ABIE53_003969 at 121.049 kb on - strand, within ABIE53_003969 at 121.159 kb on - strand, within ABIE53_003969 at 121.211 kb on + strand, within ABIE53_003969 at 121.211 kb on + strand, within ABIE53_003969 at 121.211 kb on + strand, within ABIE53_003969 at 121.212 kb on - strand, within ABIE53_003969 at 121.212 kb on - strand, within ABIE53_003969 at 121.212 kb on - strand, within ABIE53_003969 at 121.214 kb on - strand, within ABIE53_003969 at 121.214 kb on - strand, within ABIE53_003969 at 121.237 kb on + strand, within ABIE53_003969
Per-strain Table
Position Strand Gene LocusTag Fraction biofilm; attached cells from 96 well plate assay in R2A remove 117,662 + +0.9 117,662 + +0.1 117,662 + +1.9 117,663 - +2.4 117,663 - -1.2 117,663 - +0.6 117,962 + ABIE53_003965 0.32 +0.2 117,963 - ABIE53_003965 0.32 -1.2 118,073 + ABIE53_003965 0.43 -0.1 118,074 - ABIE53_003965 0.44 -0.9 118,244 + ABIE53_003965 0.61 -0.1 118,244 + ABIE53_003965 0.61 +1.2 118,930 + ABIE53_003966 0.19 +0.7 119,155 + ABIE53_003966 0.33 +0.4 119,155 + ABIE53_003966 0.33 -0.6 119,241 + ABIE53_003966 0.38 +0.4 119,242 - ABIE53_003966 0.38 -0.4 119,325 + ABIE53_003966 0.43 +1.1 119,338 + ABIE53_003966 0.44 -0.2 119,339 - ABIE53_003966 0.44 +0.9 119,378 - ABIE53_003966 0.46 +1.2 119,378 - ABIE53_003966 0.46 +0.3 119,378 - ABIE53_003966 0.46 -1.1 119,438 + ABIE53_003966 0.50 +1.2 119,610 + ABIE53_003966 0.61 +0.8 119,611 - ABIE53_003966 0.61 -1.1 119,611 - ABIE53_003966 0.61 +2.9 119,941 + ABIE53_003966 0.81 -1.9 119,941 + ABIE53_003966 0.81 -0.1 119,942 - ABIE53_003966 0.81 -0.8 120,010 - ABIE53_003966 0.85 +0.9 120,010 - ABIE53_003966 0.85 -0.6 120,010 - ABIE53_003966 0.85 -0.8 120,010 - ABIE53_003966 0.85 -1.6 120,123 - +0.6 120,123 - -0.5 120,126 + -0.1 120,126 + +3.1 120,249 + -0.9 120,249 + +0.1 120,250 - -1.8 120,250 - -0.6 120,250 - +0.9 120,250 - -0.8 120,327 + ABIE53_003967 0.63 -0.0 120,407 + +0.3 120,408 - -1.0 120,521 - ABIE53_003968 0.27 -0.7 120,571 + ABIE53_003968 0.40 -1.1 120,571 + ABIE53_003968 0.40 +1.7 120,572 - ABIE53_003968 0.40 -0.1 120,752 - ABIE53_003968 0.88 -1.1 120,816 + +0.4 120,872 - -0.4 120,872 - -0.3 120,872 - +0.9 120,872 - +0.5 121,046 + ABIE53_003969 0.17 -0.4 121,047 - ABIE53_003969 0.18 +0.9 121,047 - ABIE53_003969 0.18 -0.2 121,047 - ABIE53_003969 0.18 -0.4 121,047 - ABIE53_003969 0.18 +1.5 121,047 - ABIE53_003969 0.18 -0.2 121,048 + ABIE53_003969 0.18 +0.0 121,048 + ABIE53_003969 0.18 +0.4 121,049 - ABIE53_003969 0.18 +1.9 121,159 - ABIE53_003969 0.25 +1.5 121,211 + ABIE53_003969 0.29 +0.5 121,211 + ABIE53_003969 0.29 -0.2 121,211 + ABIE53_003969 0.29 +0.9 121,212 - ABIE53_003969 0.29 +0.9 121,212 - ABIE53_003969 0.29 -1.1 121,212 - ABIE53_003969 0.29 +0.1 121,214 - ABIE53_003969 0.29 +1.9 121,214 - ABIE53_003969 0.29 -0.1 121,237 + ABIE53_003969 0.31 -2.0
Or see this region's nucleotide sequence