Experiment: KB with Karanjin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2198 and Psyr_2199 are separated by 33 nucleotides Psyr_2199 and Psyr_2200 overlap by 4 nucleotides Psyr_2200 and Psyr_2201 are separated by 22 nucleotides Psyr_2201 and Psyr_2202 are separated by 2 nucleotides
Psyr_2198: Psyr_2198 - Urease, beta subunit:Urease, gamma subunit, at 2,556,211 to 2,556,906
_2198
Psyr_2199: Psyr_2199 - Urease accessory protein UreF, at 2,556,940 to 2,557,671
_2199
Psyr_2200: Psyr_2200 - UreE urease accessory, N-terminal, at 2,557,668 to 2,558,108
_2200
Psyr_2201: Psyr_2201 - amino acid/amide ABC transporter ATP-binding protein 2, HAAT family, at 2,558,131 to 2,558,820
_2201
Psyr_2202: Psyr_2202 - ABC transporter, at 2,558,823 to 2,559,560
_2202
Position (kb)
2557
2558
2559 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2556.866 kb on + strand at 2556.929 kb on + strand at 2556.930 kb on - strand at 2557.801 kb on + strand, within Psyr_2200 at 2557.849 kb on + strand, within Psyr_2200 at 2557.850 kb on - strand, within Psyr_2200 at 2558.474 kb on + strand, within Psyr_2201 at 2558.486 kb on + strand, within Psyr_2201 at 2558.486 kb on + strand, within Psyr_2201 at 2558.487 kb on - strand, within Psyr_2201 at 2558.609 kb on + strand, within Psyr_2201 at 2558.792 kb on + strand at 2558.792 kb on + strand at 2558.792 kb on + strand at 2558.792 kb on + strand at 2558.793 kb on - strand at 2558.885 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Karanjin 10 mM remove 2,556,866 + -2.1 2,556,929 + -0.3 2,556,930 - -1.0 2,557,801 + Psyr_2200 0.30 -1.6 2,557,849 + Psyr_2200 0.41 +0.6 2,557,850 - Psyr_2200 0.41 -1.0 2,558,474 + Psyr_2201 0.50 +1.0 2,558,486 + Psyr_2201 0.51 -0.5 2,558,486 + Psyr_2201 0.51 -0.4 2,558,487 - Psyr_2201 0.52 -1.1 2,558,609 + Psyr_2201 0.69 +0.1 2,558,792 + -0.1 2,558,792 + -0.9 2,558,792 + -0.1 2,558,792 + -0.3 2,558,793 - -0.0 2,558,885 - +3.7
Or see this region's nucleotide sequence