Experiment: KB with Irigenin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2520 and Psyr_2521 overlap by 4 nucleotides Psyr_2521 and Psyr_2522 are separated by 7 nucleotides Psyr_2522 and Psyr_2523 overlap by 4 nucleotides
Psyr_2520: Psyr_2520 - SOS-response transcriptional repressor, LexA, at 2,928,985 to 2,929,602
_2520
Psyr_2521: Psyr_2521 - conserved hypothetical protein, at 2,929,599 to 2,930,222
_2521
Psyr_2522: Psyr_2522 - conserved hypothetical protein, at 2,930,230 to 2,931,660
_2522
Psyr_2523: Psyr_2523 - DNA polymerase III, alpha subunit, at 2,931,657 to 2,934,752
_2523
Position (kb)
2930
2931
2932 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2929.336 kb on + strand, within Psyr_2520 at 2929.410 kb on + strand, within Psyr_2520 at 2929.863 kb on + strand, within Psyr_2521 at 2929.863 kb on + strand, within Psyr_2521 at 2929.863 kb on + strand, within Psyr_2521 at 2929.863 kb on + strand, within Psyr_2521 at 2929.864 kb on - strand, within Psyr_2521 at 2929.864 kb on - strand, within Psyr_2521 at 2929.864 kb on - strand, within Psyr_2521 at 2929.864 kb on - strand, within Psyr_2521 at 2930.229 kb on + strand at 2930.229 kb on + strand at 2930.229 kb on + strand at 2930.230 kb on - strand at 2930.494 kb on + strand, within Psyr_2522 at 2930.884 kb on - strand, within Psyr_2522 at 2930.884 kb on - strand, within Psyr_2522 at 2930.945 kb on + strand, within Psyr_2522 at 2930.945 kb on + strand, within Psyr_2522 at 2930.946 kb on - strand, within Psyr_2522 at 2930.946 kb on - strand, within Psyr_2522 at 2930.946 kb on - strand, within Psyr_2522 at 2930.946 kb on - strand, within Psyr_2522 at 2930.946 kb on - strand, within Psyr_2522 at 2930.946 kb on - strand, within Psyr_2522 at 2930.946 kb on - strand, within Psyr_2522 at 2930.946 kb on - strand, within Psyr_2522 at 2931.417 kb on + strand, within Psyr_2522 at 2931.418 kb on - strand, within Psyr_2522 at 2931.565 kb on + strand at 2931.568 kb on + strand at 2931.568 kb on + strand at 2931.569 kb on - strand at 2931.569 kb on - strand at 2931.806 kb on - strand at 2931.916 kb on - strand at 2931.973 kb on - strand, within Psyr_2523 at 2932.389 kb on - strand, within Psyr_2523 at 2932.428 kb on + strand, within Psyr_2523 at 2932.428 kb on + strand, within Psyr_2523 at 2932.428 kb on + strand, within Psyr_2523 at 2932.429 kb on - strand, within Psyr_2523 at 2932.429 kb on - strand, within Psyr_2523 at 2932.429 kb on - strand, within Psyr_2523 at 2932.429 kb on - strand, within Psyr_2523 at 2932.470 kb on - strand, within Psyr_2523 at 2932.470 kb on - strand, within Psyr_2523 at 2932.596 kb on + strand, within Psyr_2523 at 2932.597 kb on - strand, within Psyr_2523 at 2932.597 kb on - strand, within Psyr_2523 at 2932.597 kb on - strand, within Psyr_2523
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Irigenin 10 mM remove 2,929,336 + Psyr_2520 0.57 +0.8 2,929,410 + Psyr_2520 0.69 -0.5 2,929,863 + Psyr_2521 0.42 +0.9 2,929,863 + Psyr_2521 0.42 +0.1 2,929,863 + Psyr_2521 0.42 +0.9 2,929,863 + Psyr_2521 0.42 -0.3 2,929,864 - Psyr_2521 0.42 +0.1 2,929,864 - Psyr_2521 0.42 +0.8 2,929,864 - Psyr_2521 0.42 +0.4 2,929,864 - Psyr_2521 0.42 +0.9 2,930,229 + +2.0 2,930,229 + +1.1 2,930,229 + +0.8 2,930,230 - -0.1 2,930,494 + Psyr_2522 0.18 +0.4 2,930,884 - Psyr_2522 0.46 +0.5 2,930,884 - Psyr_2522 0.46 -0.3 2,930,945 + Psyr_2522 0.50 +0.3 2,930,945 + Psyr_2522 0.50 -1.4 2,930,946 - Psyr_2522 0.50 +0.5 2,930,946 - Psyr_2522 0.50 +0.9 2,930,946 - Psyr_2522 0.50 +0.5 2,930,946 - Psyr_2522 0.50 +1.5 2,930,946 - Psyr_2522 0.50 -2.6 2,930,946 - Psyr_2522 0.50 -1.4 2,930,946 - Psyr_2522 0.50 -0.0 2,930,946 - Psyr_2522 0.50 +0.3 2,931,417 + Psyr_2522 0.83 +0.9 2,931,418 - Psyr_2522 0.83 +1.6 2,931,565 + +0.9 2,931,568 + +0.8 2,931,568 + +0.1 2,931,569 - +0.5 2,931,569 - -1.5 2,931,806 - +0.7 2,931,916 - +1.4 2,931,973 - Psyr_2523 0.10 +0.1 2,932,389 - Psyr_2523 0.24 +0.1 2,932,428 + Psyr_2523 0.25 -2.0 2,932,428 + Psyr_2523 0.25 -0.9 2,932,428 + Psyr_2523 0.25 -0.1 2,932,429 - Psyr_2523 0.25 -1.4 2,932,429 - Psyr_2523 0.25 +1.0 2,932,429 - Psyr_2523 0.25 -1.3 2,932,429 - Psyr_2523 0.25 -0.0 2,932,470 - Psyr_2523 0.26 -0.1 2,932,470 - Psyr_2523 0.26 -0.4 2,932,596 + Psyr_2523 0.30 +0.7 2,932,597 - Psyr_2523 0.30 -1.4 2,932,597 - Psyr_2523 0.30 -1.0 2,932,597 - Psyr_2523 0.30 +0.4
Or see this region's nucleotide sequence