Experiment: KB with Irigenin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2476 and Psyr_2477 are separated by 192 nucleotides Psyr_2477 and Psyr_2478 are separated by 59 nucleotides Psyr_2478 and Psyr_2479 are separated by 5 nucleotides
Psyr_2476: Psyr_2476 - Exodeoxyribonuclease III, at 2,870,547 to 2,871,359
_2476
Psyr_2477: Psyr_2477 - conserved hypothetical protein, at 2,871,552 to 2,872,223
_2477
Psyr_2478: Psyr_2478 - surface antigen (D15):Surface antigen variable number, at 2,872,283 to 2,874,007
_2478
Psyr_2479: Psyr_2479 - Protein of unknown function DUF490, at 2,874,013 to 2,877,693
_2479
Position (kb)
2872
2873
2874
2875 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2871.458 kb on + strand at 2871.551 kb on + strand at 2871.551 kb on + strand at 2871.552 kb on - strand at 2871.552 kb on - strand at 2871.604 kb on - strand at 2871.903 kb on + strand, within Psyr_2477 at 2871.904 kb on - strand, within Psyr_2477 at 2871.906 kb on + strand, within Psyr_2477 at 2871.906 kb on + strand, within Psyr_2477 at 2871.906 kb on + strand, within Psyr_2477 at 2871.907 kb on - strand, within Psyr_2477 at 2871.907 kb on - strand, within Psyr_2477 at 2871.916 kb on + strand, within Psyr_2477 at 2871.916 kb on + strand, within Psyr_2477 at 2871.943 kb on + strand, within Psyr_2477 at 2871.944 kb on - strand, within Psyr_2477 at 2872.107 kb on + strand, within Psyr_2477 at 2872.107 kb on + strand, within Psyr_2477 at 2872.107 kb on + strand, within Psyr_2477 at 2872.107 kb on + strand, within Psyr_2477 at 2872.107 kb on + strand, within Psyr_2477 at 2872.108 kb on - strand, within Psyr_2477 at 2872.213 kb on + strand at 2872.214 kb on - strand at 2872.214 kb on - strand at 2872.269 kb on + strand at 2872.269 kb on + strand at 2872.277 kb on - strand at 2872.409 kb on + strand at 2872.409 kb on + strand at 2872.409 kb on + strand at 2872.409 kb on + strand at 2872.409 kb on + strand at 2872.409 kb on + strand at 2872.409 kb on + strand at 2872.410 kb on - strand at 2872.506 kb on - strand, within Psyr_2478 at 2872.508 kb on + strand, within Psyr_2478 at 2872.931 kb on + strand, within Psyr_2478 at 2872.932 kb on - strand, within Psyr_2478 at 2872.932 kb on - strand, within Psyr_2478 at 2872.932 kb on - strand, within Psyr_2478 at 2872.983 kb on - strand, within Psyr_2478 at 2873.097 kb on - strand, within Psyr_2478 at 2873.280 kb on - strand, within Psyr_2478 at 2873.459 kb on + strand, within Psyr_2478 at 2873.460 kb on - strand, within Psyr_2478 at 2873.460 kb on - strand, within Psyr_2478 at 2873.460 kb on - strand, within Psyr_2478 at 2873.460 kb on - strand, within Psyr_2478 at 2873.498 kb on + strand, within Psyr_2478 at 2873.655 kb on - strand, within Psyr_2478 at 2873.721 kb on - strand, within Psyr_2478 at 2873.771 kb on + strand, within Psyr_2478 at 2873.772 kb on - strand, within Psyr_2478 at 2873.801 kb on + strand, within Psyr_2478 at 2873.801 kb on + strand, within Psyr_2478 at 2873.923 kb on - strand at 2874.062 kb on + strand at 2874.062 kb on + strand at 2874.408 kb on + strand, within Psyr_2479 at 2874.409 kb on - strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.410 kb on + strand, within Psyr_2479 at 2874.411 kb on - strand, within Psyr_2479 at 2874.411 kb on - strand, within Psyr_2479 at 2874.411 kb on - strand, within Psyr_2479 at 2874.411 kb on - strand, within Psyr_2479 at 2874.411 kb on - strand, within Psyr_2479 at 2874.411 kb on - strand, within Psyr_2479 at 2874.411 kb on - strand, within Psyr_2479 at 2874.411 kb on - strand, within Psyr_2479 at 2874.411 kb on - strand, within Psyr_2479 at 2874.860 kb on + strand, within Psyr_2479 at 2874.861 kb on - strand, within Psyr_2479
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Irigenin 10 mM remove 2,871,458 + +0.1 2,871,551 + +0.8 2,871,551 + +0.6 2,871,552 - +0.3 2,871,552 - +0.2 2,871,604 - +0.4 2,871,903 + Psyr_2477 0.52 -0.5 2,871,904 - Psyr_2477 0.52 +0.1 2,871,906 + Psyr_2477 0.53 +1.5 2,871,906 + Psyr_2477 0.53 +0.3 2,871,906 + Psyr_2477 0.53 -0.6 2,871,907 - Psyr_2477 0.53 +1.4 2,871,907 - Psyr_2477 0.53 +0.7 2,871,916 + Psyr_2477 0.54 +0.5 2,871,916 + Psyr_2477 0.54 -1.5 2,871,943 + Psyr_2477 0.58 +0.4 2,871,944 - Psyr_2477 0.58 -0.8 2,872,107 + Psyr_2477 0.83 -0.8 2,872,107 + Psyr_2477 0.83 +1.8 2,872,107 + Psyr_2477 0.83 -0.3 2,872,107 + Psyr_2477 0.83 -1.1 2,872,107 + Psyr_2477 0.83 -0.8 2,872,108 - Psyr_2477 0.83 +0.7 2,872,213 + -0.5 2,872,214 - +0.3 2,872,214 - -1.0 2,872,269 + -1.5 2,872,269 + +1.6 2,872,277 - +0.1 2,872,409 + +0.2 2,872,409 + +2.9 2,872,409 + -0.4 2,872,409 + -0.5 2,872,409 + +0.5 2,872,409 + -0.7 2,872,409 + -0.3 2,872,410 - -0.2 2,872,506 - Psyr_2478 0.13 +1.1 2,872,508 + Psyr_2478 0.13 -0.9 2,872,931 + Psyr_2478 0.38 +0.1 2,872,932 - Psyr_2478 0.38 -0.1 2,872,932 - Psyr_2478 0.38 -0.1 2,872,932 - Psyr_2478 0.38 -0.2 2,872,983 - Psyr_2478 0.41 +0.1 2,873,097 - Psyr_2478 0.47 +1.6 2,873,280 - Psyr_2478 0.58 -1.0 2,873,459 + Psyr_2478 0.68 -0.1 2,873,460 - Psyr_2478 0.68 +0.4 2,873,460 - Psyr_2478 0.68 +1.0 2,873,460 - Psyr_2478 0.68 +1.2 2,873,460 - Psyr_2478 0.68 +0.4 2,873,498 + Psyr_2478 0.70 +0.7 2,873,655 - Psyr_2478 0.80 +0.1 2,873,721 - Psyr_2478 0.83 -0.8 2,873,771 + Psyr_2478 0.86 +1.5 2,873,772 - Psyr_2478 0.86 +1.1 2,873,801 + Psyr_2478 0.88 -0.1 2,873,801 + Psyr_2478 0.88 +0.6 2,873,923 - -0.4 2,874,062 + +2.3 2,874,062 + -3.1 2,874,408 + Psyr_2479 0.11 -0.3 2,874,409 - Psyr_2479 0.11 -0.1 2,874,410 + Psyr_2479 0.11 -1.0 2,874,410 + Psyr_2479 0.11 +0.2 2,874,410 + Psyr_2479 0.11 -0.3 2,874,410 + Psyr_2479 0.11 +0.4 2,874,410 + Psyr_2479 0.11 -1.3 2,874,410 + Psyr_2479 0.11 -0.7 2,874,410 + Psyr_2479 0.11 +0.7 2,874,410 + Psyr_2479 0.11 -1.7 2,874,410 + Psyr_2479 0.11 +1.1 2,874,410 + Psyr_2479 0.11 +0.1 2,874,410 + Psyr_2479 0.11 +0.8 2,874,410 + Psyr_2479 0.11 +1.4 2,874,410 + Psyr_2479 0.11 +0.1 2,874,410 + Psyr_2479 0.11 -0.2 2,874,411 - Psyr_2479 0.11 +0.1 2,874,411 - Psyr_2479 0.11 +0.8 2,874,411 - Psyr_2479 0.11 +0.5 2,874,411 - Psyr_2479 0.11 -1.8 2,874,411 - Psyr_2479 0.11 -0.5 2,874,411 - Psyr_2479 0.11 -0.2 2,874,411 - Psyr_2479 0.11 -0.2 2,874,411 - Psyr_2479 0.11 +0.8 2,874,411 - Psyr_2479 0.11 +1.2 2,874,860 + Psyr_2479 0.23 -0.5 2,874,861 - Psyr_2479 0.23 +0.5
Or see this region's nucleotide sequence