Experiment: KB with Irigenin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2133 and Psyr_2134 are separated by 77 nucleotides Psyr_2134 and Psyr_2135 are separated by 96 nucleotides Psyr_2135 and Psyr_2136 are separated by 75 nucleotides
Psyr_2133: Psyr_2133 - Pseudouridine synthase, at 2,493,154 to 2,494,095
_2133
Psyr_2134: Psyr_2134 - protein of unknown function DUF903, at 2,494,173 to 2,494,397
_2134
Psyr_2135: Psyr_2135 - molybdenum cofactor guanylyltransferase, at 2,494,494 to 2,495,099
_2135
Psyr_2136: Psyr_2136 - Molybdenum cofactor biosynthesis protein, at 2,495,175 to 2,495,714
_2136
Position (kb)
2494
2495 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2493.185 kb on - strand at 2493.185 kb on - strand at 2493.247 kb on - strand at 2493.247 kb on - strand at 2493.253 kb on + strand, within Psyr_2133 at 2493.253 kb on + strand, within Psyr_2133 at 2493.253 kb on + strand, within Psyr_2133 at 2493.254 kb on - strand, within Psyr_2133 at 2493.254 kb on - strand, within Psyr_2133 at 2493.254 kb on - strand, within Psyr_2133 at 2493.254 kb on - strand, within Psyr_2133 at 2493.421 kb on + strand, within Psyr_2133 at 2493.541 kb on + strand, within Psyr_2133 at 2493.541 kb on + strand, within Psyr_2133 at 2493.542 kb on - strand, within Psyr_2133 at 2493.594 kb on + strand, within Psyr_2133 at 2493.595 kb on - strand, within Psyr_2133 at 2493.595 kb on - strand, within Psyr_2133 at 2493.595 kb on - strand, within Psyr_2133 at 2493.674 kb on - strand, within Psyr_2133 at 2493.917 kb on + strand, within Psyr_2133 at 2493.918 kb on - strand, within Psyr_2133 at 2493.918 kb on - strand, within Psyr_2133 at 2493.918 kb on - strand, within Psyr_2133 at 2493.918 kb on - strand, within Psyr_2133 at 2494.097 kb on - strand at 2494.190 kb on + strand at 2494.190 kb on + strand at 2494.191 kb on - strand at 2494.194 kb on + strand at 2494.194 kb on + strand at 2494.195 kb on - strand at 2494.195 kb on - strand at 2494.268 kb on - strand, within Psyr_2134 at 2494.268 kb on - strand, within Psyr_2134 at 2494.398 kb on + strand at 2494.466 kb on - strand at 2494.467 kb on + strand at 2494.467 kb on + strand at 2494.676 kb on + strand, within Psyr_2135 at 2494.677 kb on - strand, within Psyr_2135 at 2494.680 kb on + strand, within Psyr_2135 at 2494.785 kb on + strand, within Psyr_2135 at 2494.785 kb on + strand, within Psyr_2135 at 2494.842 kb on + strand, within Psyr_2135 at 2494.842 kb on + strand, within Psyr_2135 at 2494.843 kb on - strand, within Psyr_2135 at 2494.843 kb on - strand, within Psyr_2135 at 2495.066 kb on + strand at 2495.066 kb on + strand at 2495.067 kb on - strand at 2495.136 kb on + strand at 2495.136 kb on + strand at 2495.136 kb on + strand at 2495.136 kb on + strand at 2495.137 kb on - strand at 2495.137 kb on - strand at 2495.137 kb on - strand at 2495.138 kb on + strand at 2495.138 kb on + strand at 2495.139 kb on - strand at 2495.139 kb on - strand at 2495.139 kb on - strand at 2495.139 kb on - strand at 2495.139 kb on - strand at 2495.139 kb on - strand at 2495.204 kb on - strand at 2495.210 kb on + strand at 2495.210 kb on + strand at 2495.211 kb on - strand at 2495.211 kb on - strand at 2495.341 kb on - strand, within Psyr_2136
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Irigenin 10 mM remove 2,493,185 - +0.5 2,493,185 - -0.8 2,493,247 - -0.4 2,493,247 - +0.1 2,493,253 + Psyr_2133 0.11 +1.3 2,493,253 + Psyr_2133 0.11 -0.4 2,493,253 + Psyr_2133 0.11 +0.1 2,493,254 - Psyr_2133 0.11 -0.1 2,493,254 - Psyr_2133 0.11 +0.2 2,493,254 - Psyr_2133 0.11 -2.1 2,493,254 - Psyr_2133 0.11 -1.4 2,493,421 + Psyr_2133 0.28 -0.3 2,493,541 + Psyr_2133 0.41 -0.7 2,493,541 + Psyr_2133 0.41 -0.1 2,493,542 - Psyr_2133 0.41 +0.2 2,493,594 + Psyr_2133 0.47 -0.1 2,493,595 - Psyr_2133 0.47 +0.5 2,493,595 - Psyr_2133 0.47 +1.7 2,493,595 - Psyr_2133 0.47 +0.7 2,493,674 - Psyr_2133 0.55 -0.9 2,493,917 + Psyr_2133 0.81 -0.7 2,493,918 - Psyr_2133 0.81 -0.1 2,493,918 - Psyr_2133 0.81 +0.1 2,493,918 - Psyr_2133 0.81 -2.4 2,493,918 - Psyr_2133 0.81 +0.3 2,494,097 - -0.3 2,494,190 + -2.7 2,494,190 + -1.5 2,494,191 - +1.5 2,494,194 + +1.5 2,494,194 + -0.2 2,494,195 - +0.8 2,494,195 - +1.2 2,494,268 - Psyr_2134 0.42 +0.1 2,494,268 - Psyr_2134 0.42 -0.7 2,494,398 + +0.1 2,494,466 - +0.4 2,494,467 + +0.5 2,494,467 + +0.4 2,494,676 + Psyr_2135 0.30 -0.7 2,494,677 - Psyr_2135 0.30 +0.3 2,494,680 + Psyr_2135 0.31 -0.1 2,494,785 + Psyr_2135 0.48 -0.1 2,494,785 + Psyr_2135 0.48 +0.1 2,494,842 + Psyr_2135 0.57 +0.1 2,494,842 + Psyr_2135 0.57 -0.9 2,494,843 - Psyr_2135 0.58 -0.1 2,494,843 - Psyr_2135 0.58 -0.8 2,495,066 + -0.1 2,495,066 + -0.5 2,495,067 - +0.5 2,495,136 + +0.1 2,495,136 + -0.3 2,495,136 + -0.3 2,495,136 + -0.3 2,495,137 - -0.4 2,495,137 - -0.9 2,495,137 - -2.4 2,495,138 + -1.3 2,495,138 + +2.9 2,495,139 - -0.7 2,495,139 - +0.9 2,495,139 - +0.7 2,495,139 - -1.3 2,495,139 - -1.4 2,495,139 - +0.4 2,495,204 - -0.3 2,495,210 + -0.5 2,495,210 + -0.4 2,495,211 - -0.4 2,495,211 - -0.9 2,495,341 - Psyr_2136 0.31 +0.3
Or see this region's nucleotide sequence