Experiment: KB with Irigenin 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1527 and Psyr_1528 are separated by 2 nucleotides Psyr_1528 and Psyr_1529 are separated by 231 nucleotides Psyr_1529 and Psyr_1530 are separated by 1 nucleotides Psyr_1530 and Psyr_1531 overlap by 4 nucleotides
Psyr_1527: Psyr_1527 - conserved hypothetical protein, at 1,717,367 to 1,719,127
_1527
Psyr_1528: Psyr_1528 - Arc-like DNA binding protein, at 1,719,130 to 1,719,393
_1528
Psyr_1529: Psyr_1529 - hypothetical protein, at 1,719,625 to 1,720,032
_1529
Psyr_1530: Psyr_1530 - hypothetical protein, at 1,720,034 to 1,720,375
_1530
Psyr_1531: Psyr_1531 - replicative DNA helicase (phage and plasmid), at 1,720,372 to 1,721,718
_1531
Position (kb) 1719
1720
1721 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1718.646 kb on + strand, within Psyr_1527 at 1718.646 kb on + strand, within Psyr_1527 at 1718.646 kb on + strand, within Psyr_1527 at 1718.646 kb on + strand, within Psyr_1527 at 1718.647 kb on - strand, within Psyr_1527 at 1718.647 kb on - strand, within Psyr_1527 at 1718.647 kb on - strand, within Psyr_1527 at 1718.647 kb on - strand, within Psyr_1527 at 1718.647 kb on - strand, within Psyr_1527 at 1718.647 kb on - strand, within Psyr_1527 at 1718.647 kb on - strand, within Psyr_1527 at 1718.741 kb on - strand, within Psyr_1527 at 1718.818 kb on - strand, within Psyr_1527 at 1718.845 kb on + strand, within Psyr_1527 at 1718.868 kb on + strand, within Psyr_1527 at 1718.868 kb on + strand, within Psyr_1527 at 1718.868 kb on + strand, within Psyr_1527 at 1718.869 kb on - strand, within Psyr_1527 at 1718.869 kb on - strand, within Psyr_1527 at 1718.895 kb on - strand, within Psyr_1527 at 1718.900 kb on - strand, within Psyr_1527 at 1718.949 kb on + strand, within Psyr_1527 at 1718.949 kb on + strand, within Psyr_1527 at 1718.949 kb on + strand, within Psyr_1527 at 1718.950 kb on - strand, within Psyr_1527 at 1718.950 kb on - strand, within Psyr_1527 at 1718.950 kb on - strand, within Psyr_1527 at 1718.950 kb on - strand, within Psyr_1527 at 1718.950 kb on - strand, within Psyr_1527 at 1719.022 kb on + strand at 1719.022 kb on + strand at 1719.023 kb on - strand at 1719.023 kb on - strand at 1719.023 kb on - strand at 1719.026 kb on + strand at 1719.026 kb on + strand at 1719.026 kb on + strand at 1719.026 kb on + strand at 1719.026 kb on + strand at 1719.027 kb on - strand at 1719.027 kb on - strand at 1719.027 kb on - strand at 1719.027 kb on - strand at 1719.027 kb on - strand at 1719.027 kb on - strand at 1719.083 kb on + strand at 1719.083 kb on + strand at 1719.084 kb on - strand at 1719.084 kb on - strand at 1719.131 kb on + strand at 1719.207 kb on + strand, within Psyr_1528 at 1719.207 kb on + strand, within Psyr_1528 at 1719.207 kb on + strand, within Psyr_1528 at 1719.256 kb on + strand, within Psyr_1528 at 1719.256 kb on + strand, within Psyr_1528 at 1719.324 kb on + strand, within Psyr_1528 at 1719.521 kb on - strand at 1719.521 kb on - strand at 1719.521 kb on - strand at 1719.521 kb on - strand at 1719.521 kb on - strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.613 kb on + strand at 1719.614 kb on - strand at 1719.614 kb on - strand at 1719.614 kb on - strand at 1719.614 kb on - strand at 1719.614 kb on - strand at 1719.614 kb on - strand at 1719.614 kb on - strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.619 kb on + strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.620 kb on - strand at 1719.678 kb on - strand, within Psyr_1529 at 1719.689 kb on - strand, within Psyr_1529 at 1719.689 kb on - strand, within Psyr_1529 at 1719.868 kb on - strand, within Psyr_1529 at 1719.931 kb on + strand, within Psyr_1529 at 1719.931 kb on + strand, within Psyr_1529 at 1719.931 kb on + strand, within Psyr_1529 at 1719.931 kb on + strand, within Psyr_1529 at 1719.931 kb on + strand, within Psyr_1529 at 1719.932 kb on - strand, within Psyr_1529 at 1719.932 kb on - strand, within Psyr_1529 at 1719.932 kb on - strand, within Psyr_1529 at 1719.932 kb on - strand, within Psyr_1529 at 1719.932 kb on - strand, within Psyr_1529 at 1719.932 kb on - strand, within Psyr_1529 at 1719.932 kb on - strand, within Psyr_1529 at 1719.932 kb on - strand, within Psyr_1529 at 1719.932 kb on - strand, within Psyr_1529 at 1719.932 kb on - strand, within Psyr_1529 at 1719.935 kb on + strand, within Psyr_1529 at 1719.935 kb on + strand, within Psyr_1529 at 1719.935 kb on + strand, within Psyr_1529 at 1719.936 kb on - strand, within Psyr_1529 at 1719.936 kb on - strand, within Psyr_1529 at 1719.936 kb on - strand, within Psyr_1529 at 1720.014 kb on + strand at 1720.022 kb on + strand at 1720.023 kb on - strand at 1720.023 kb on - strand at 1720.023 kb on - strand at 1720.024 kb on + strand at 1720.024 kb on + strand at 1720.024 kb on + strand at 1720.024 kb on + strand at 1720.024 kb on + strand at 1720.024 kb on + strand at 1720.024 kb on + strand at 1720.024 kb on + strand at 1720.024 kb on + strand at 1720.024 kb on + strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.025 kb on - strand at 1720.144 kb on + strand, within Psyr_1530 at 1720.145 kb on - strand, within Psyr_1530 at 1720.145 kb on - strand, within Psyr_1530 at 1720.173 kb on + strand, within Psyr_1530 at 1720.173 kb on + strand, within Psyr_1530 at 1720.173 kb on + strand, within Psyr_1530 at 1720.174 kb on - strand, within Psyr_1530 at 1720.241 kb on + strand, within Psyr_1530 at 1720.346 kb on + strand at 1720.360 kb on + strand at 1720.523 kb on + strand, within Psyr_1531 at 1720.524 kb on - strand, within Psyr_1531 at 1720.524 kb on - strand, within Psyr_1531 at 1720.540 kb on + strand, within Psyr_1531 at 1720.540 kb on + strand, within Psyr_1531 at 1720.540 kb on + strand, within Psyr_1531 at 1720.540 kb on + strand, within Psyr_1531 at 1720.540 kb on + strand, within Psyr_1531 at 1720.541 kb on - strand, within Psyr_1531 at 1720.541 kb on - strand, within Psyr_1531 at 1720.541 kb on - strand, within Psyr_1531 at 1720.541 kb on - strand, within Psyr_1531 at 1720.602 kb on + strand, within Psyr_1531 at 1720.602 kb on + strand, within Psyr_1531 at 1720.602 kb on + strand, within Psyr_1531 at 1720.603 kb on - strand, within Psyr_1531 at 1720.603 kb on - strand, within Psyr_1531 at 1720.603 kb on - strand, within Psyr_1531 at 1720.702 kb on + strand, within Psyr_1531 at 1720.702 kb on + strand, within Psyr_1531 at 1720.702 kb on + strand, within Psyr_1531 at 1720.703 kb on - strand, within Psyr_1531 at 1720.703 kb on - strand, within Psyr_1531 at 1720.703 kb on - strand, within Psyr_1531 at 1720.735 kb on - strand, within Psyr_1531 at 1720.858 kb on + strand, within Psyr_1531 at 1720.858 kb on + strand, within Psyr_1531 at 1720.858 kb on + strand, within Psyr_1531 at 1720.858 kb on + strand, within Psyr_1531 at 1720.859 kb on - strand, within Psyr_1531 at 1720.859 kb on - strand, within Psyr_1531 at 1720.859 kb on - strand, within Psyr_1531 at 1720.859 kb on - strand, within Psyr_1531 at 1720.859 kb on - strand at 1720.875 kb on - strand, within Psyr_1531 at 1720.985 kb on + strand, within Psyr_1531 at 1720.985 kb on + strand, within Psyr_1531 at 1720.986 kb on - strand, within Psyr_1531
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Irigenin 10 mM remove 1,718,646 + Psyr_1527 0.73 +0.3 1,718,646 + Psyr_1527 0.73 -0.8 1,718,646 + Psyr_1527 0.73 -0.6 1,718,646 + Psyr_1527 0.73 +1.2 1,718,647 - Psyr_1527 0.73 -0.1 1,718,647 - Psyr_1527 0.73 -1.3 1,718,647 - Psyr_1527 0.73 -0.5 1,718,647 - Psyr_1527 0.73 +1.0 1,718,647 - Psyr_1527 0.73 -0.3 1,718,647 - Psyr_1527 0.73 +0.3 1,718,647 - Psyr_1527 0.73 +0.6 1,718,741 - Psyr_1527 0.78 +0.0 1,718,818 - Psyr_1527 0.82 +0.3 1,718,845 + Psyr_1527 0.84 -0.3 1,718,868 + Psyr_1527 0.85 +1.5 1,718,868 + Psyr_1527 0.85 +1.5 1,718,868 + Psyr_1527 0.85 -0.5 1,718,869 - Psyr_1527 0.85 +0.3 1,718,869 - Psyr_1527 0.85 -1.4 1,718,895 - Psyr_1527 0.87 +0.1 1,718,900 - Psyr_1527 0.87 +1.4 1,718,949 + Psyr_1527 0.90 +0.3 1,718,949 + Psyr_1527 0.90 +0.4 1,718,949 + Psyr_1527 0.90 -0.2 1,718,950 - Psyr_1527 0.90 -0.4 1,718,950 - Psyr_1527 0.90 +0.8 1,718,950 - Psyr_1527 0.90 -0.6 1,718,950 - Psyr_1527 0.90 -0.6 1,718,950 - Psyr_1527 0.90 -2.0 1,719,022 + +0.7 1,719,022 + +0.4 1,719,023 - -0.8 1,719,023 - -0.1 1,719,023 - -1.2 1,719,026 + -0.4 1,719,026 + +0.3 1,719,026 + -2.5 1,719,026 + -0.0 1,719,026 + +1.2 1,719,027 - -0.8 1,719,027 - +0.2 1,719,027 - +0.8 1,719,027 - -0.4 1,719,027 - +0.5 1,719,027 - +0.8 1,719,083 + -0.2 1,719,083 + -0.0 1,719,084 - +0.2 1,719,084 - -1.1 1,719,131 + +0.1 1,719,207 + Psyr_1528 0.29 +0.5 1,719,207 + Psyr_1528 0.29 +0.9 1,719,207 + Psyr_1528 0.29 -0.7 1,719,256 + Psyr_1528 0.48 -2.8 1,719,256 + Psyr_1528 0.48 +2.2 1,719,324 + Psyr_1528 0.73 -1.0 1,719,521 - +1.2 1,719,521 - +0.2 1,719,521 - +0.2 1,719,521 - -0.8 1,719,521 - -0.4 1,719,613 + -1.0 1,719,613 + +0.1 1,719,613 + +1.9 1,719,613 + -0.2 1,719,613 + +2.0 1,719,613 + -0.9 1,719,613 + -1.6 1,719,613 + +0.3 1,719,613 + -0.8 1,719,613 + -0.6 1,719,613 + +0.3 1,719,613 + +0.9 1,719,613 + +0.5 1,719,613 + -1.8 1,719,613 + -1.7 1,719,613 + -1.0 1,719,614 - -0.5 1,719,614 - +0.2 1,719,614 - -1.0 1,719,614 - +0.2 1,719,614 - +0.4 1,719,614 - -1.3 1,719,614 - +0.2 1,719,619 + +0.5 1,719,619 + -0.7 1,719,619 + -1.1 1,719,619 + +0.6 1,719,619 + +0.3 1,719,619 + +1.4 1,719,619 + +0.0 1,719,619 + +0.3 1,719,619 + -2.2 1,719,619 + +0.7 1,719,619 + +0.7 1,719,619 + -0.2 1,719,619 + +1.0 1,719,619 + -0.2 1,719,619 + -0.4 1,719,619 + +0.7 1,719,620 - -0.1 1,719,620 - -1.4 1,719,620 - -1.7 1,719,620 - -0.0 1,719,620 - -0.8 1,719,620 - +0.2 1,719,620 - -0.9 1,719,620 - +0.2 1,719,620 - +1.5 1,719,620 - -0.4 1,719,620 - -1.4 1,719,620 - -0.3 1,719,620 - -0.1 1,719,620 - -1.5 1,719,620 - +1.9 1,719,620 - +0.7 1,719,620 - -0.1 1,719,620 - +0.7 1,719,678 - Psyr_1529 0.13 +0.2 1,719,689 - Psyr_1529 0.16 +0.8 1,719,689 - Psyr_1529 0.16 -0.8 1,719,868 - Psyr_1529 0.60 -0.3 1,719,931 + Psyr_1529 0.75 +0.4 1,719,931 + Psyr_1529 0.75 -1.1 1,719,931 + Psyr_1529 0.75 -0.1 1,719,931 + Psyr_1529 0.75 -0.5 1,719,931 + Psyr_1529 0.75 -0.4 1,719,932 - Psyr_1529 0.75 -1.2 1,719,932 - Psyr_1529 0.75 -2.4 1,719,932 - Psyr_1529 0.75 -0.6 1,719,932 - Psyr_1529 0.75 +0.5 1,719,932 - Psyr_1529 0.75 -0.7 1,719,932 - Psyr_1529 0.75 -0.6 1,719,932 - Psyr_1529 0.75 -0.6 1,719,932 - Psyr_1529 0.75 -0.6 1,719,932 - Psyr_1529 0.75 -0.7 1,719,932 - Psyr_1529 0.75 +1.7 1,719,935 + Psyr_1529 0.76 +1.9 1,719,935 + Psyr_1529 0.76 -0.3 1,719,935 + Psyr_1529 0.76 -0.4 1,719,936 - Psyr_1529 0.76 -0.1 1,719,936 - Psyr_1529 0.76 -0.4 1,719,936 - Psyr_1529 0.76 +1.2 1,720,014 + -0.2 1,720,022 + -0.9 1,720,023 - +0.8 1,720,023 - -0.0 1,720,023 - +0.3 1,720,024 + +0.5 1,720,024 + -0.1 1,720,024 + -0.8 1,720,024 + -1.2 1,720,024 + +0.9 1,720,024 + -0.1 1,720,024 + -0.8 1,720,024 + +0.3 1,720,024 + -0.8 1,720,024 + -1.1 1,720,025 - -2.4 1,720,025 - +0.4 1,720,025 - -2.1 1,720,025 - +0.4 1,720,025 - -0.4 1,720,025 - +1.0 1,720,025 - -1.3 1,720,025 - -0.3 1,720,025 - +0.1 1,720,025 - -1.2 1,720,025 - +1.2 1,720,025 - -0.2 1,720,025 - +0.6 1,720,025 - -0.5 1,720,025 - +0.4 1,720,144 + Psyr_1530 0.32 +0.1 1,720,145 - Psyr_1530 0.32 -1.5 1,720,145 - Psyr_1530 0.32 -0.3 1,720,173 + Psyr_1530 0.41 +1.2 1,720,173 + Psyr_1530 0.41 +1.5 1,720,173 + Psyr_1530 0.41 +2.7 1,720,174 - Psyr_1530 0.41 -0.4 1,720,241 + Psyr_1530 0.61 -1.3 1,720,346 + +1.0 1,720,360 + -0.3 1,720,523 + Psyr_1531 0.11 -1.3 1,720,524 - Psyr_1531 0.11 +1.2 1,720,524 - Psyr_1531 0.11 -0.3 1,720,540 + Psyr_1531 0.12 +1.6 1,720,540 + Psyr_1531 0.12 +1.2 1,720,540 + Psyr_1531 0.12 -0.6 1,720,540 + Psyr_1531 0.12 +1.4 1,720,540 + Psyr_1531 0.12 +0.8 1,720,541 - Psyr_1531 0.13 -2.1 1,720,541 - Psyr_1531 0.13 -0.9 1,720,541 - Psyr_1531 0.13 -0.4 1,720,541 - Psyr_1531 0.13 -0.6 1,720,602 + Psyr_1531 0.17 +0.5 1,720,602 + Psyr_1531 0.17 -0.1 1,720,602 + Psyr_1531 0.17 +2.2 1,720,603 - Psyr_1531 0.17 -1.1 1,720,603 - Psyr_1531 0.17 +0.6 1,720,603 - Psyr_1531 0.17 -0.8 1,720,702 + Psyr_1531 0.24 +1.0 1,720,702 + Psyr_1531 0.24 -1.0 1,720,702 + Psyr_1531 0.24 +0.2 1,720,703 - Psyr_1531 0.25 +0.4 1,720,703 - Psyr_1531 0.25 -1.8 1,720,703 - Psyr_1531 0.25 -0.3 1,720,735 - Psyr_1531 0.27 +1.4 1,720,858 + Psyr_1531 0.36 +0.6 1,720,858 + Psyr_1531 0.36 -1.1 1,720,858 + Psyr_1531 0.36 -0.1 1,720,858 + Psyr_1531 0.36 -0.6 1,720,859 - Psyr_1531 0.36 -0.7 1,720,859 - Psyr_1531 0.36 +0.6 1,720,859 - Psyr_1531 0.36 -0.1 1,720,859 - Psyr_1531 0.36 +1.0 1,720,859 - -1.7 1,720,875 - Psyr_1531 0.37 +0.2 1,720,985 + Psyr_1531 0.46 -0.7 1,720,985 + Psyr_1531 0.46 -0.1 1,720,986 - Psyr_1531 0.46 -0.4
Or see this region's nucleotide sequence