Experiment: carbon source Sucrose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4161 and Pf1N1B4_4162 are separated by 2 nucleotides Pf1N1B4_4162 and Pf1N1B4_4163 are separated by 3 nucleotides
Pf1N1B4_4161 - UPF0192 protein all5100 precursor, at 4,605,236 to 4,609,810
_4161
Pf1N1B4_4162 - FIG00954181: hypothetical protein, at 4,609,813 to 4,611,432
_4162
Pf1N1B4_4163 - FIG00638718: hypothetical protein, at 4,611,436 to 4,612,251
_4163
Position (kb)
4609
4610
4611
4612 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4609.212 kb on - strand, within Pf1N1B4_4161 at 4609.212 kb on - strand, within Pf1N1B4_4161 at 4609.219 kb on + strand, within Pf1N1B4_4161 at 4609.232 kb on + strand, within Pf1N1B4_4161 at 4609.232 kb on + strand, within Pf1N1B4_4161 at 4609.232 kb on + strand, within Pf1N1B4_4161 at 4609.232 kb on + strand, within Pf1N1B4_4161 at 4609.232 kb on + strand, within Pf1N1B4_4161 at 4609.232 kb on + strand, within Pf1N1B4_4161 at 4609.232 kb on + strand, within Pf1N1B4_4161 at 4609.232 kb on + strand, within Pf1N1B4_4161 at 4609.232 kb on + strand, within Pf1N1B4_4161 at 4609.233 kb on - strand, within Pf1N1B4_4161 at 4609.748 kb on + strand at 4610.038 kb on + strand, within Pf1N1B4_4162 at 4610.071 kb on + strand, within Pf1N1B4_4162 at 4610.071 kb on + strand, within Pf1N1B4_4162 at 4610.071 kb on + strand at 4610.072 kb on - strand, within Pf1N1B4_4162 at 4610.134 kb on + strand at 4610.135 kb on - strand, within Pf1N1B4_4162 at 4610.509 kb on + strand, within Pf1N1B4_4162 at 4610.510 kb on - strand, within Pf1N1B4_4162 at 4610.758 kb on + strand, within Pf1N1B4_4162 at 4610.758 kb on + strand, within Pf1N1B4_4162 at 4610.836 kb on - strand, within Pf1N1B4_4162 at 4610.839 kb on + strand, within Pf1N1B4_4162 at 4610.992 kb on + strand, within Pf1N1B4_4162 at 4610.992 kb on + strand, within Pf1N1B4_4162 at 4610.993 kb on - strand, within Pf1N1B4_4162 at 4610.993 kb on - strand at 4610.993 kb on - strand, within Pf1N1B4_4162 at 4611.068 kb on - strand, within Pf1N1B4_4162 at 4611.092 kb on - strand, within Pf1N1B4_4162 at 4611.296 kb on - strand at 4611.296 kb on - strand at 4611.296 kb on - strand at 4611.346 kb on + strand at 4611.347 kb on - strand at 4611.395 kb on - strand at 4611.395 kb on - strand at 4611.395 kb on - strand at 4611.486 kb on - strand at 4611.486 kb on - strand at 4612.051 kb on + strand, within Pf1N1B4_4163 at 4612.052 kb on - strand, within Pf1N1B4_4163 at 4612.052 kb on - strand, within Pf1N1B4_4163 at 4612.060 kb on + strand, within Pf1N1B4_4163 at 4612.061 kb on - strand, within Pf1N1B4_4163 at 4612.061 kb on - strand, within Pf1N1B4_4163 at 4612.069 kb on + strand, within Pf1N1B4_4163 at 4612.069 kb on + strand, within Pf1N1B4_4163 at 4612.069 kb on + strand, within Pf1N1B4_4163 at 4612.070 kb on - strand, within Pf1N1B4_4163 at 4612.070 kb on - strand, within Pf1N1B4_4163 at 4612.070 kb on - strand, within Pf1N1B4_4163 at 4612.070 kb on - strand, within Pf1N1B4_4163 at 4612.072 kb on + strand, within Pf1N1B4_4163 at 4612.240 kb on - strand at 4612.378 kb on + strand at 4612.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM remove 4,609,212 - Pf1N1B4_4161 0.87 +0.8 4,609,212 - Pf1N1B4_4161 0.87 +0.7 4,609,219 + Pf1N1B4_4161 0.87 +1.4 4,609,232 + Pf1N1B4_4161 0.87 +1.7 4,609,232 + Pf1N1B4_4161 0.87 +0.9 4,609,232 + Pf1N1B4_4161 0.87 -1.0 4,609,232 + Pf1N1B4_4161 0.87 -0.9 4,609,232 + Pf1N1B4_4161 0.87 -1.2 4,609,232 + Pf1N1B4_4161 0.87 +0.7 4,609,232 + Pf1N1B4_4161 0.87 -1.8 4,609,232 + Pf1N1B4_4161 0.87 -1.9 4,609,232 + Pf1N1B4_4161 0.87 -2.2 4,609,233 - Pf1N1B4_4161 0.87 -0.3 4,609,748 + +0.7 4,610,038 + Pf1N1B4_4162 0.14 +1.3 4,610,071 + Pf1N1B4_4162 0.16 -0.9 4,610,071 + Pf1N1B4_4162 0.16 -0.3 4,610,071 + +1.9 4,610,072 - Pf1N1B4_4162 0.16 -1.8 4,610,134 + +0.8 4,610,135 - Pf1N1B4_4162 0.20 -0.8 4,610,509 + Pf1N1B4_4162 0.43 +0.5 4,610,510 - Pf1N1B4_4162 0.43 +0.0 4,610,758 + Pf1N1B4_4162 0.58 -0.2 4,610,758 + Pf1N1B4_4162 0.58 -0.5 4,610,836 - Pf1N1B4_4162 0.63 +2.5 4,610,839 + Pf1N1B4_4162 0.63 -0.3 4,610,992 + Pf1N1B4_4162 0.73 +2.8 4,610,992 + Pf1N1B4_4162 0.73 +0.6 4,610,993 - Pf1N1B4_4162 0.73 +1.0 4,610,993 - -0.3 4,610,993 - Pf1N1B4_4162 0.73 -0.7 4,611,068 - Pf1N1B4_4162 0.77 +2.3 4,611,092 - Pf1N1B4_4162 0.79 +0.2 4,611,296 - +1.8 4,611,296 - +0.5 4,611,296 - +1.5 4,611,346 + -2.0 4,611,347 - -1.7 4,611,395 - +0.8 4,611,395 - -1.2 4,611,395 - -0.0 4,611,486 - -1.8 4,611,486 - -1.5 4,612,051 + Pf1N1B4_4163 0.75 +0.2 4,612,052 - Pf1N1B4_4163 0.75 -2.3 4,612,052 - Pf1N1B4_4163 0.75 -2.5 4,612,060 + Pf1N1B4_4163 0.76 -0.5 4,612,061 - Pf1N1B4_4163 0.77 -3.1 4,612,061 - Pf1N1B4_4163 0.77 -2.9 4,612,069 + Pf1N1B4_4163 0.78 +0.8 4,612,069 + Pf1N1B4_4163 0.78 +0.4 4,612,069 + Pf1N1B4_4163 0.78 -2.5 4,612,070 - Pf1N1B4_4163 0.78 +1.0 4,612,070 - Pf1N1B4_4163 0.78 -0.2 4,612,070 - Pf1N1B4_4163 0.78 +1.6 4,612,070 - Pf1N1B4_4163 0.78 +0.7 4,612,072 + Pf1N1B4_4163 0.78 -2.1 4,612,240 - +1.7 4,612,378 + +0.8 4,612,412 - -1.5
Or see this region's nucleotide sequence