Experiment: carbon source Sucrose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2889 and Pf1N1B4_2890 are separated by 101 nucleotides Pf1N1B4_2890 and Pf1N1B4_2891 overlap by 8 nucleotides Pf1N1B4_2891 and Pf1N1B4_2892 are separated by 101 nucleotides Pf1N1B4_2892 and Pf1N1B4_2893 are separated by 183 nucleotides
Pf1N1B4_2889 - FIG00954491: hypothetical protein, at 3,243,701 to 3,245,044
_2889
Pf1N1B4_2890 - Periplasmic protein TonB, links inner and outer membranes, at 3,245,146 to 3,245,925
_2890
Pf1N1B4_2891 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 3,245,918 to 3,246,370
_2891
Pf1N1B4_2892 - Phosphotransferase system, HPr-related proteins, at 3,246,472 to 3,246,876
_2892
Pf1N1B4_2893 - Carbonic anhydrase (EC 4.2.1.1), at 3,247,060 to 3,247,704
_2893
Position (kb)
3245
3246
3247 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3245.004 kb on - strand at 3245.018 kb on + strand at 3245.018 kb on + strand at 3245.019 kb on - strand at 3245.019 kb on - strand at 3245.019 kb on - strand at 3245.069 kb on + strand at 3245.070 kb on - strand at 3245.122 kb on + strand at 3245.122 kb on + strand at 3245.122 kb on + strand at 3245.931 kb on + strand at 3245.970 kb on + strand, within Pf1N1B4_2891 at 3245.993 kb on + strand, within Pf1N1B4_2891 at 3245.993 kb on + strand at 3246.041 kb on + strand, within Pf1N1B4_2891 at 3246.042 kb on - strand, within Pf1N1B4_2891 at 3246.200 kb on + strand, within Pf1N1B4_2891 at 3246.201 kb on - strand, within Pf1N1B4_2891 at 3246.266 kb on + strand, within Pf1N1B4_2891 at 3246.266 kb on + strand, within Pf1N1B4_2891 at 3246.266 kb on + strand, within Pf1N1B4_2891 at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.894 kb on + strand at 3246.895 kb on - strand at 3246.895 kb on - strand at 3246.895 kb on - strand at 3246.901 kb on + strand at 3246.902 kb on - strand at 3246.904 kb on + strand at 3246.905 kb on - strand at 3246.964 kb on + strand at 3246.964 kb on + strand at 3246.964 kb on + strand at 3246.965 kb on - strand at 3246.965 kb on - strand at 3246.965 kb on - strand at 3246.965 kb on - strand at 3246.986 kb on + strand at 3247.070 kb on + strand at 3247.070 kb on + strand at 3247.070 kb on + strand at 3247.071 kb on - strand at 3247.071 kb on - strand at 3247.086 kb on + strand at 3247.145 kb on + strand, within Pf1N1B4_2893 at 3247.146 kb on - strand, within Pf1N1B4_2893 at 3247.146 kb on - strand, within Pf1N1B4_2893 at 3247.168 kb on + strand, within Pf1N1B4_2893 at 3247.168 kb on + strand, within Pf1N1B4_2893 at 3247.168 kb on + strand, within Pf1N1B4_2893 at 3247.252 kb on - strand, within Pf1N1B4_2893 at 3247.307 kb on - strand, within Pf1N1B4_2893 at 3247.370 kb on + strand, within Pf1N1B4_2893 at 3247.370 kb on + strand, within Pf1N1B4_2893
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM remove 3,245,004 - -1.7 3,245,018 + -0.5 3,245,018 + -1.6 3,245,019 - +4.1 3,245,019 - -1.0 3,245,019 - -2.0 3,245,069 + +3.3 3,245,070 - -0.2 3,245,122 + +1.8 3,245,122 + +1.4 3,245,122 + -1.4 3,245,931 + -1.5 3,245,970 + Pf1N1B4_2891 0.11 -0.2 3,245,993 + Pf1N1B4_2891 0.17 -0.7 3,245,993 + -0.5 3,246,041 + Pf1N1B4_2891 0.27 -0.7 3,246,042 - Pf1N1B4_2891 0.27 +0.4 3,246,200 + Pf1N1B4_2891 0.62 -0.5 3,246,201 - Pf1N1B4_2891 0.62 +3.0 3,246,266 + Pf1N1B4_2891 0.77 -1.5 3,246,266 + Pf1N1B4_2891 0.77 +0.5 3,246,266 + Pf1N1B4_2891 0.77 -0.7 3,246,355 + +0.4 3,246,355 + -1.5 3,246,355 + +0.5 3,246,355 + -1.9 3,246,355 + -1.7 3,246,355 + +1.0 3,246,355 + -1.8 3,246,355 + +1.4 3,246,355 + +0.8 3,246,355 + +0.3 3,246,356 - -0.6 3,246,356 - +1.9 3,246,356 - +2.1 3,246,356 - -2.6 3,246,356 - +1.1 3,246,356 - -0.6 3,246,356 - +0.4 3,246,356 - -1.4 3,246,890 - -2.5 3,246,890 - -1.4 3,246,890 - -1.1 3,246,890 - +0.7 3,246,890 - -0.7 3,246,890 - -2.2 3,246,894 + +0.7 3,246,895 - -0.5 3,246,895 - -1.7 3,246,895 - +1.8 3,246,901 + +3.3 3,246,902 - -0.3 3,246,904 + -1.4 3,246,905 - -1.0 3,246,964 + -3.0 3,246,964 + +1.5 3,246,964 + -1.4 3,246,965 - -1.4 3,246,965 - -0.4 3,246,965 - +1.5 3,246,965 - -0.6 3,246,986 + +3.0 3,247,070 + -1.6 3,247,070 + -1.5 3,247,070 + +1.7 3,247,071 - -0.9 3,247,071 - -1.5 3,247,086 + -1.1 3,247,145 + Pf1N1B4_2893 0.13 -1.0 3,247,146 - Pf1N1B4_2893 0.13 -1.7 3,247,146 - Pf1N1B4_2893 0.13 -2.7 3,247,168 + Pf1N1B4_2893 0.17 -2.6 3,247,168 + Pf1N1B4_2893 0.17 -2.1 3,247,168 + Pf1N1B4_2893 0.17 -1.1 3,247,252 - Pf1N1B4_2893 0.30 -1.9 3,247,307 - Pf1N1B4_2893 0.38 -2.0 3,247,370 + Pf1N1B4_2893 0.48 -1.6 3,247,370 + Pf1N1B4_2893 0.48 -0.3
Or see this region's nucleotide sequence