Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2721

Experiment: carbon source Sucrose 0.25mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2720 and Pf1N1B4_2721 are separated by 185 nucleotidesPf1N1B4_2721 and Pf1N1B4_2722 are separated by 71 nucleotidesPf1N1B4_2722 and Pf1N1B4_2723 overlap by 6 nucleotides Pf1N1B4_2720 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 3,058,059 to 3,059,708 _2720 Pf1N1B4_2721 - AMP nucleosidase (EC 3.2.2.4), at 3,059,894 to 3,061,357 _2721 Pf1N1B4_2722 - Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70), at 3,061,429 to 3,062,064 _2722 Pf1N1B4_2723 - FIG00954142: hypothetical protein, at 3,062,059 to 3,062,268 _2723 Position (kb) 3059 3060 3061 3062Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 3059.220 kb on + strand, within Pf1N1B4_2720at 3059.220 kb on + strand, within Pf1N1B4_2720at 3059.220 kb on + strand, within Pf1N1B4_2720at 3059.221 kb on - strand, within Pf1N1B4_2720at 3059.221 kb on - strand, within Pf1N1B4_2720at 3059.287 kb on - strand, within Pf1N1B4_2720at 3059.377 kb on + strand, within Pf1N1B4_2720at 3059.377 kb on + strand, within Pf1N1B4_2720at 3059.377 kb on + strand, within Pf1N1B4_2720at 3059.377 kb on + strand, within Pf1N1B4_2720at 3059.378 kb on - strand, within Pf1N1B4_2720at 3059.378 kb on - strand, within Pf1N1B4_2720at 3059.378 kb on - strand, within Pf1N1B4_2720at 3059.566 kb on + strandat 3059.566 kb on + strandat 3059.566 kb on + strandat 3059.567 kb on - strandat 3059.640 kb on + strandat 3059.640 kb on + strandat 3059.640 kb on + strandat 3059.641 kb on - strandat 3059.733 kb on + strandat 3059.734 kb on - strandat 3059.734 kb on - strandat 3059.734 kb on - strandat 3059.734 kb on - strandat 3059.795 kb on + strandat 3059.796 kb on - strandat 3059.796 kb on - strandat 3059.908 kb on + strandat 3059.999 kb on + strandat 3060.000 kb on - strandat 3060.072 kb on - strand, within Pf1N1B4_2721at 3060.072 kb on - strand, within Pf1N1B4_2721at 3060.072 kb on - strand, within Pf1N1B4_2721at 3060.072 kb on - strand, within Pf1N1B4_2721at 3060.072 kb on - strand, within Pf1N1B4_2721at 3060.072 kb on - strand, within Pf1N1B4_2721at 3060.110 kb on + strand, within Pf1N1B4_2721at 3060.111 kb on - strand, within Pf1N1B4_2721at 3060.137 kb on + strand, within Pf1N1B4_2721at 3060.137 kb on + strand, within Pf1N1B4_2721at 3060.137 kb on + strand, within Pf1N1B4_2721at 3060.137 kb on + strand, within Pf1N1B4_2721at 3060.138 kb on - strand, within Pf1N1B4_2721at 3060.138 kb on - strand, within Pf1N1B4_2721at 3060.138 kb on - strand, within Pf1N1B4_2721at 3060.138 kb on - strand, within Pf1N1B4_2721at 3060.176 kb on + strandat 3060.176 kb on + strand, within Pf1N1B4_2721at 3060.176 kb on + strand, within Pf1N1B4_2721at 3060.176 kb on + strand, within Pf1N1B4_2721at 3060.177 kb on - strand, within Pf1N1B4_2721at 3060.177 kb on - strand, within Pf1N1B4_2721at 3060.263 kb on + strand, within Pf1N1B4_2721at 3060.337 kb on + strand, within Pf1N1B4_2721at 3060.380 kb on + strand, within Pf1N1B4_2721at 3060.381 kb on - strand, within Pf1N1B4_2721at 3060.464 kb on + strand, within Pf1N1B4_2721at 3060.465 kb on - strand, within Pf1N1B4_2721at 3060.465 kb on - strand, within Pf1N1B4_2721at 3060.465 kb on - strand, within Pf1N1B4_2721at 3060.515 kb on + strand, within Pf1N1B4_2721at 3060.515 kb on + strand, within Pf1N1B4_2721at 3060.515 kb on + strand, within Pf1N1B4_2721at 3060.516 kb on - strand, within Pf1N1B4_2721at 3060.689 kb on + strandat 3060.854 kb on + strand, within Pf1N1B4_2721at 3060.854 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.872 kb on + strand, within Pf1N1B4_2721at 3060.873 kb on - strand, within Pf1N1B4_2721at 3060.873 kb on - strand, within Pf1N1B4_2721at 3060.873 kb on - strand, within Pf1N1B4_2721at 3060.873 kb on - strand, within Pf1N1B4_2721at 3061.034 kb on + strand, within Pf1N1B4_2721at 3061.034 kb on + strand, within Pf1N1B4_2721at 3061.034 kb on + strand, within Pf1N1B4_2721at 3061.034 kb on + strand, within Pf1N1B4_2721at 3061.035 kb on - strand, within Pf1N1B4_2721at 3061.035 kb on - strand, within Pf1N1B4_2721at 3061.035 kb on - strand, within Pf1N1B4_2721at 3061.235 kb on + strandat 3061.235 kb on + strandat 3061.236 kb on - strandat 3061.236 kb on - strandat 3061.236 kb on - strandat 3061.236 kb on - strandat 3061.236 kb on - strandat 3061.390 kb on + strandat 3061.412 kb on + strandat 3061.413 kb on - strandat 3061.732 kb on + strand, within Pf1N1B4_2722at 3061.732 kb on + strand, within Pf1N1B4_2722at 3061.732 kb on + strand, within Pf1N1B4_2722at 3061.732 kb on + strand, within Pf1N1B4_2722at 3061.733 kb on - strand, within Pf1N1B4_2722at 3061.733 kb on - strand, within Pf1N1B4_2722at 3061.733 kb on - strand, within Pf1N1B4_2722at 3061.733 kb on - strand, within Pf1N1B4_2722at 3061.733 kb on - strand, within Pf1N1B4_2722at 3061.733 kb on - strand, within Pf1N1B4_2722at 3061.809 kb on + strand, within Pf1N1B4_2722at 3062.060 kb on + strandat 3062.061 kb on - strandat 3062.062 kb on + strandat 3062.062 kb on + strandat 3062.062 kb on + strandat 3062.062 kb on + strandat 3062.062 kb on + strandat 3062.062 kb on + strandat 3062.063 kb on - strandat 3062.063 kb on - strandat 3062.131 kb on + strand, within Pf1N1B4_2723at 3062.132 kb on - strand, within Pf1N1B4_2723at 3062.294 kb on + strandat 3062.294 kb on + strandat 3062.295 kb on - strandat 3062.309 kb on + strandat 3062.309 kb on + strandat 3062.310 kb on - strandat 3062.310 kb on - strandat 3062.310 kb on - strandat 3062.311 kb on + strandat 3062.311 kb on + strandat 3062.312 kb on - strandat 3062.312 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM
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3,059,220 + Pf1N1B4_2720 0.70 +2.6
3,059,220 + Pf1N1B4_2720 0.70 +0.7
3,059,220 + Pf1N1B4_2720 0.70 +1.0
3,059,221 - Pf1N1B4_2720 0.70 -0.4
3,059,221 - Pf1N1B4_2720 0.70 -2.2
3,059,287 - Pf1N1B4_2720 0.74 -0.4
3,059,377 + Pf1N1B4_2720 0.80 +0.7
3,059,377 + Pf1N1B4_2720 0.80 -0.9
3,059,377 + Pf1N1B4_2720 0.80 -0.4
3,059,377 + Pf1N1B4_2720 0.80 +0.0
3,059,378 - Pf1N1B4_2720 0.80 -0.6
3,059,378 - Pf1N1B4_2720 0.80 -0.2
3,059,378 - Pf1N1B4_2720 0.80 -1.0
3,059,566 + +1.8
3,059,566 + -1.7
3,059,566 + +1.1
3,059,567 - +0.6
3,059,640 + +0.7
3,059,640 + +1.1
3,059,640 + -0.8
3,059,641 - +0.4
3,059,733 + +0.7
3,059,734 - -1.7
3,059,734 - +2.0
3,059,734 - -0.5
3,059,734 - -0.9
3,059,795 + +1.3
3,059,796 - -0.6
3,059,796 - -0.8
3,059,908 + +0.7
3,059,999 + +3.3
3,060,000 - -1.8
3,060,072 - Pf1N1B4_2721 0.12 +4.3
3,060,072 - Pf1N1B4_2721 0.12 -1.4
3,060,072 - Pf1N1B4_2721 0.12 +1.8
3,060,072 - Pf1N1B4_2721 0.12 +2.0
3,060,072 - Pf1N1B4_2721 0.12 +0.5
3,060,072 - Pf1N1B4_2721 0.12 -1.5
3,060,110 + Pf1N1B4_2721 0.15 +1.9
3,060,111 - Pf1N1B4_2721 0.15 +1.4
3,060,137 + Pf1N1B4_2721 0.17 -0.8
3,060,137 + Pf1N1B4_2721 0.17 +3.1
3,060,137 + Pf1N1B4_2721 0.17 -2.8
3,060,137 + Pf1N1B4_2721 0.17 +0.3
3,060,138 - Pf1N1B4_2721 0.17 -1.0
3,060,138 - Pf1N1B4_2721 0.17 -0.6
3,060,138 - Pf1N1B4_2721 0.17 -2.0
3,060,138 - Pf1N1B4_2721 0.17 +2.0
3,060,176 + -1.3
3,060,176 + Pf1N1B4_2721 0.19 +1.3
3,060,176 + Pf1N1B4_2721 0.19 -0.3
3,060,176 + Pf1N1B4_2721 0.19 +0.9
3,060,177 - Pf1N1B4_2721 0.19 -3.4
3,060,177 - Pf1N1B4_2721 0.19 +0.1
3,060,263 + Pf1N1B4_2721 0.25 +0.9
3,060,337 + Pf1N1B4_2721 0.30 -2.5
3,060,380 + Pf1N1B4_2721 0.33 -2.4
3,060,381 - Pf1N1B4_2721 0.33 +0.9
3,060,464 + Pf1N1B4_2721 0.39 -1.2
3,060,465 - Pf1N1B4_2721 0.39 -1.2
3,060,465 - Pf1N1B4_2721 0.39 +0.1
3,060,465 - Pf1N1B4_2721 0.39 +0.3
3,060,515 + Pf1N1B4_2721 0.42 +1.1
3,060,515 + Pf1N1B4_2721 0.42 -1.4
3,060,515 + Pf1N1B4_2721 0.42 -3.2
3,060,516 - Pf1N1B4_2721 0.42 +0.7
3,060,689 + -0.1
3,060,854 + Pf1N1B4_2721 0.66 -1.7
3,060,854 + Pf1N1B4_2721 0.66 -0.9
3,060,872 + Pf1N1B4_2721 0.67 -1.4
3,060,872 + Pf1N1B4_2721 0.67 +2.6
3,060,872 + Pf1N1B4_2721 0.67 -1.3
3,060,872 + Pf1N1B4_2721 0.67 +0.3
3,060,872 + Pf1N1B4_2721 0.67 +1.3
3,060,872 + Pf1N1B4_2721 0.67 -1.4
3,060,872 + Pf1N1B4_2721 0.67 -2.0
3,060,873 - Pf1N1B4_2721 0.67 -0.4
3,060,873 - Pf1N1B4_2721 0.67 -0.6
3,060,873 - Pf1N1B4_2721 0.67 +0.3
3,060,873 - Pf1N1B4_2721 0.67 -3.0
3,061,034 + Pf1N1B4_2721 0.78 +1.3
3,061,034 + Pf1N1B4_2721 0.78 +1.4
3,061,034 + Pf1N1B4_2721 0.78 +0.3
3,061,034 + Pf1N1B4_2721 0.78 -0.3
3,061,035 - Pf1N1B4_2721 0.78 -1.4
3,061,035 - Pf1N1B4_2721 0.78 -0.4
3,061,035 - Pf1N1B4_2721 0.78 +0.9
3,061,235 + -0.3
3,061,235 + -1.6
3,061,236 - -0.6
3,061,236 - +0.0
3,061,236 - -1.6
3,061,236 - +1.1
3,061,236 - +2.1
3,061,390 + -1.9
3,061,412 + -1.2
3,061,413 - +1.0
3,061,732 + Pf1N1B4_2722 0.48 -0.4
3,061,732 + Pf1N1B4_2722 0.48 -1.7
3,061,732 + Pf1N1B4_2722 0.48 +1.1
3,061,732 + Pf1N1B4_2722 0.48 -1.5
3,061,733 - Pf1N1B4_2722 0.48 +1.7
3,061,733 - Pf1N1B4_2722 0.48 -0.0
3,061,733 - Pf1N1B4_2722 0.48 +0.1
3,061,733 - Pf1N1B4_2722 0.48 +1.0
3,061,733 - Pf1N1B4_2722 0.48 -3.2
3,061,733 - Pf1N1B4_2722 0.48 -1.0
3,061,809 + Pf1N1B4_2722 0.60 +0.9
3,062,060 + -1.1
3,062,061 - -0.1
3,062,062 + -0.5
3,062,062 + -3.1
3,062,062 + -1.3
3,062,062 + +0.6
3,062,062 + +2.1
3,062,062 + +4.0
3,062,063 - -0.9
3,062,063 - -0.4
3,062,131 + Pf1N1B4_2723 0.34 -2.0
3,062,132 - Pf1N1B4_2723 0.35 +0.8
3,062,294 + -1.1
3,062,294 + -1.4
3,062,295 - -2.3
3,062,309 + +0.0
3,062,309 + -1.9
3,062,310 - -1.4
3,062,310 - -1.1
3,062,310 - -2.0
3,062,311 + -1.1
3,062,311 + -1.5
3,062,312 - +1.1
3,062,312 - +0.7

Or see this region's nucleotide sequence