Experiment: carbon source Sucrose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_231 and Pf1N1B4_232 are separated by 47 nucleotides Pf1N1B4_232 and Pf1N1B4_233 overlap by 17 nucleotides
Pf1N1B4_231 - Sensor histidine kinase, at 292,071 to 292,964
_231
Pf1N1B4_232 - DNA-binding response regulator, LuxR family, at 293,012 to 293,620
_232
Pf1N1B4_233 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 293,604 to 294,536
_233
Position (kb)
293
294 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 292.070 kb on + strand at 292.071 kb on - strand at 292.074 kb on + strand at 292.075 kb on - strand at 292.075 kb on - strand at 292.095 kb on - strand at 292.095 kb on - strand at 292.095 kb on - strand at 292.148 kb on + strand at 292.148 kb on + strand at 292.149 kb on - strand at 292.193 kb on - strand, within Pf1N1B4_231 at 292.224 kb on - strand, within Pf1N1B4_231 at 292.561 kb on + strand, within Pf1N1B4_231 at 292.561 kb on + strand at 292.562 kb on - strand, within Pf1N1B4_231 at 292.580 kb on + strand, within Pf1N1B4_231 at 292.580 kb on + strand, within Pf1N1B4_231 at 292.580 kb on + strand, within Pf1N1B4_231 at 292.581 kb on - strand, within Pf1N1B4_231 at 292.682 kb on + strand, within Pf1N1B4_231 at 292.682 kb on + strand, within Pf1N1B4_231 at 292.683 kb on - strand at 292.684 kb on + strand, within Pf1N1B4_231 at 292.684 kb on + strand, within Pf1N1B4_231 at 292.684 kb on + strand, within Pf1N1B4_231 at 292.706 kb on + strand, within Pf1N1B4_231 at 292.849 kb on + strand, within Pf1N1B4_231 at 292.849 kb on + strand, within Pf1N1B4_231 at 292.931 kb on - strand at 292.949 kb on + strand at 292.950 kb on - strand at 292.969 kb on + strand at 292.969 kb on + strand at 292.970 kb on - strand at 292.974 kb on + strand at 292.974 kb on + strand at 292.974 kb on + strand at 292.974 kb on + strand at 292.975 kb on - strand at 292.975 kb on - strand at 292.999 kb on + strand at 293.049 kb on + strand at 293.125 kb on + strand at 293.558 kb on + strand, within Pf1N1B4_232 at 293.559 kb on - strand, within Pf1N1B4_232 at 293.559 kb on - strand, within Pf1N1B4_232 at 293.735 kb on + strand, within Pf1N1B4_233 at 293.736 kb on - strand, within Pf1N1B4_233 at 293.736 kb on - strand at 293.737 kb on + strand at 293.738 kb on - strand at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand at 293.738 kb on - strand, within Pf1N1B4_233 at 294.046 kb on + strand, within Pf1N1B4_233 at 294.046 kb on + strand, within Pf1N1B4_233 at 294.047 kb on - strand, within Pf1N1B4_233 at 294.087 kb on - strand, within Pf1N1B4_233 at 294.087 kb on - strand, within Pf1N1B4_233 at 294.087 kb on - strand, within Pf1N1B4_233 at 294.264 kb on + strand, within Pf1N1B4_233 at 294.265 kb on - strand at 294.351 kb on - strand, within Pf1N1B4_233 at 294.555 kb on + strand at 294.555 kb on + strand at 294.555 kb on + strand at 294.556 kb on - strand at 294.556 kb on - strand at 294.578 kb on + strand at 294.579 kb on - strand at 294.579 kb on - strand at 294.600 kb on + strand at 294.601 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM remove 292,070 + -2.7 292,071 - -2.0 292,074 + +2.0 292,075 - -1.0 292,075 - -1.3 292,095 - +1.0 292,095 - -0.2 292,095 - +0.8 292,148 + -1.0 292,148 + -0.7 292,149 - -1.0 292,193 - Pf1N1B4_231 0.14 +3.1 292,224 - Pf1N1B4_231 0.17 -2.3 292,561 + Pf1N1B4_231 0.55 +2.2 292,561 + -1.0 292,562 - Pf1N1B4_231 0.55 +2.3 292,580 + Pf1N1B4_231 0.57 +0.9 292,580 + Pf1N1B4_231 0.57 -2.0 292,580 + Pf1N1B4_231 0.57 -0.6 292,581 - Pf1N1B4_231 0.57 +0.1 292,682 + Pf1N1B4_231 0.68 +2.4 292,682 + Pf1N1B4_231 0.68 +0.3 292,683 - +0.1 292,684 + Pf1N1B4_231 0.69 +0.8 292,684 + Pf1N1B4_231 0.69 -0.9 292,684 + Pf1N1B4_231 0.69 +0.1 292,706 + Pf1N1B4_231 0.71 +0.6 292,849 + Pf1N1B4_231 0.87 -2.9 292,849 + Pf1N1B4_231 0.87 +1.4 292,931 - -0.4 292,949 + +3.6 292,950 - -0.6 292,969 + -0.6 292,969 + +0.5 292,970 - -0.6 292,974 + -1.3 292,974 + +2.5 292,974 + +1.7 292,974 + -3.3 292,975 - -2.2 292,975 - +1.7 292,999 + -3.2 293,049 + -1.0 293,125 + +4.0 293,558 + Pf1N1B4_232 0.90 -0.2 293,559 - Pf1N1B4_232 0.90 -2.3 293,559 - Pf1N1B4_232 0.90 -0.5 293,735 + Pf1N1B4_233 0.14 +0.3 293,736 - Pf1N1B4_233 0.14 +0.5 293,736 - +1.8 293,737 + -0.5 293,738 - -1.3 293,738 - Pf1N1B4_233 0.14 +2.1 293,738 - Pf1N1B4_233 0.14 -1.4 293,738 - Pf1N1B4_233 0.14 +1.6 293,738 - Pf1N1B4_233 0.14 -2.3 293,738 - -1.8 293,738 - Pf1N1B4_233 0.14 +0.4 294,046 + Pf1N1B4_233 0.47 +2.8 294,046 + Pf1N1B4_233 0.47 -0.6 294,047 - Pf1N1B4_233 0.47 -1.8 294,087 - Pf1N1B4_233 0.52 -2.3 294,087 - Pf1N1B4_233 0.52 -1.9 294,087 - Pf1N1B4_233 0.52 -1.4 294,264 + Pf1N1B4_233 0.71 +1.1 294,265 - +1.2 294,351 - Pf1N1B4_233 0.80 +0.4 294,555 + +0.8 294,555 + +0.8 294,555 + +0.5 294,556 - +0.2 294,556 - -1.3 294,578 + -0.8 294,579 - +1.4 294,579 - -2.0 294,600 + +0.4 294,601 - +0.7
Or see this region's nucleotide sequence