Experiment: carbon source Sucrose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1222 and Pf1N1B4_1223 are separated by 69 nucleotides Pf1N1B4_1223 and Pf1N1B4_1224 are separated by 65 nucleotides
Pf1N1B4_1222 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 1,386,353 to 1,387,276
_1222
Pf1N1B4_1223 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 1,387,346 to 1,388,428
_1223
Pf1N1B4_1224 - Probable protein farnesyltransferase beta subunit (EC 2.5.1.58), at 1,388,494 to 1,390,671
_1224
Position (kb)
1387
1388
1389 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1386.426 kb on + strand at 1386.426 kb on + strand at 1386.427 kb on - strand at 1386.427 kb on - strand at 1386.501 kb on + strand, within Pf1N1B4_1222 at 1386.501 kb on + strand, within Pf1N1B4_1222 at 1386.501 kb on + strand, within Pf1N1B4_1222 at 1386.502 kb on - strand at 1386.502 kb on - strand, within Pf1N1B4_1222 at 1386.502 kb on - strand, within Pf1N1B4_1222 at 1386.657 kb on + strand, within Pf1N1B4_1222 at 1386.657 kb on + strand, within Pf1N1B4_1222 at 1386.741 kb on + strand, within Pf1N1B4_1222 at 1386.742 kb on - strand, within Pf1N1B4_1222 at 1386.742 kb on - strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.747 kb on + strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.748 kb on - strand, within Pf1N1B4_1222 at 1386.861 kb on + strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.862 kb on - strand, within Pf1N1B4_1222 at 1386.864 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.962 kb on + strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1386.963 kb on - strand, within Pf1N1B4_1222 at 1387.162 kb on - strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.164 kb on + strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.165 kb on - strand, within Pf1N1B4_1222 at 1387.253 kb on + strand at 1387.266 kb on + strand at 1387.266 kb on + strand at 1387.266 kb on + strand at 1387.441 kb on - strand at 1387.530 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.686 kb on + strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.687 kb on - strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.722 kb on + strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.723 kb on - strand, within Pf1N1B4_1223 at 1387.783 kb on - strand, within Pf1N1B4_1223 at 1387.783 kb on - strand, within Pf1N1B4_1223 at 1387.861 kb on - strand, within Pf1N1B4_1223 at 1387.881 kb on - strand, within Pf1N1B4_1223 at 1387.971 kb on + strand, within Pf1N1B4_1223 at 1387.972 kb on - strand, within Pf1N1B4_1223 at 1387.972 kb on - strand, within Pf1N1B4_1223 at 1388.019 kb on + strand, within Pf1N1B4_1223 at 1388.019 kb on + strand, within Pf1N1B4_1223 at 1388.115 kb on + strand, within Pf1N1B4_1223 at 1388.116 kb on - strand, within Pf1N1B4_1223 at 1388.116 kb on - strand, within Pf1N1B4_1223 at 1388.116 kb on - strand, within Pf1N1B4_1223 at 1388.128 kb on + strand, within Pf1N1B4_1223 at 1388.336 kb on + strand at 1388.336 kb on + strand at 1388.337 kb on - strand at 1388.337 kb on - strand at 1388.374 kb on - strand at 1388.660 kb on + strand at 1388.660 kb on + strand at 1388.660 kb on + strand at 1388.660 kb on + strand at 1388.660 kb on + strand at 1388.660 kb on + strand at 1388.661 kb on - strand at 1388.661 kb on - strand at 1388.661 kb on - strand at 1388.661 kb on - strand at 1388.661 kb on - strand at 1388.661 kb on - strand at 1388.661 kb on - strand at 1388.684 kb on + strand at 1388.684 kb on + strand at 1388.684 kb on + strand at 1388.684 kb on + strand at 1388.684 kb on + strand at 1388.685 kb on - strand at 1388.685 kb on - strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.705 kb on + strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.706 kb on - strand at 1388.710 kb on - strand at 1388.815 kb on - strand, within Pf1N1B4_1224 at 1388.847 kb on - strand, within Pf1N1B4_1224 at 1389.012 kb on - strand, within Pf1N1B4_1224 at 1389.063 kb on - strand, within Pf1N1B4_1224 at 1389.128 kb on + strand, within Pf1N1B4_1224 at 1389.128 kb on + strand, within Pf1N1B4_1224 at 1389.128 kb on + strand, within Pf1N1B4_1224 at 1389.129 kb on - strand, within Pf1N1B4_1224 at 1389.139 kb on + strand, within Pf1N1B4_1224 at 1389.139 kb on + strand, within Pf1N1B4_1224 at 1389.139 kb on + strand, within Pf1N1B4_1224 at 1389.139 kb on + strand, within Pf1N1B4_1224 at 1389.140 kb on - strand, within Pf1N1B4_1224 at 1389.140 kb on - strand, within Pf1N1B4_1224 at 1389.140 kb on - strand, within Pf1N1B4_1224 at 1389.140 kb on - strand, within Pf1N1B4_1224 at 1389.140 kb on - strand, within Pf1N1B4_1224 at 1389.345 kb on + strand, within Pf1N1B4_1224 at 1389.345 kb on + strand, within Pf1N1B4_1224 at 1389.345 kb on + strand, within Pf1N1B4_1224 at 1389.345 kb on + strand, within Pf1N1B4_1224 at 1389.345 kb on + strand, within Pf1N1B4_1224 at 1389.346 kb on - strand, within Pf1N1B4_1224 at 1389.346 kb on - strand, within Pf1N1B4_1224 at 1389.346 kb on - strand at 1389.346 kb on - strand, within Pf1N1B4_1224
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM remove 1,386,426 + -1.2 1,386,426 + -1.6 1,386,427 - -0.9 1,386,427 - -0.4 1,386,501 + Pf1N1B4_1222 0.16 +1.9 1,386,501 + Pf1N1B4_1222 0.16 +1.4 1,386,501 + Pf1N1B4_1222 0.16 -0.0 1,386,502 - +3.6 1,386,502 - Pf1N1B4_1222 0.16 -1.0 1,386,502 - Pf1N1B4_1222 0.16 -3.1 1,386,657 + Pf1N1B4_1222 0.33 -1.1 1,386,657 + Pf1N1B4_1222 0.33 +0.1 1,386,741 + Pf1N1B4_1222 0.42 -0.5 1,386,742 - Pf1N1B4_1222 0.42 +0.2 1,386,742 - Pf1N1B4_1222 0.42 -1.6 1,386,747 + Pf1N1B4_1222 0.43 -1.4 1,386,747 + Pf1N1B4_1222 0.43 +1.8 1,386,747 + Pf1N1B4_1222 0.43 +1.8 1,386,747 + Pf1N1B4_1222 0.43 +0.2 1,386,747 + Pf1N1B4_1222 0.43 +1.8 1,386,747 + Pf1N1B4_1222 0.43 -0.7 1,386,747 + Pf1N1B4_1222 0.43 -1.2 1,386,748 - Pf1N1B4_1222 0.43 +2.6 1,386,748 - Pf1N1B4_1222 0.43 -0.3 1,386,748 - Pf1N1B4_1222 0.43 -0.2 1,386,748 - Pf1N1B4_1222 0.43 +2.0 1,386,748 - Pf1N1B4_1222 0.43 +0.6 1,386,748 - Pf1N1B4_1222 0.43 -2.0 1,386,748 - Pf1N1B4_1222 0.43 +1.9 1,386,861 + Pf1N1B4_1222 0.55 +2.0 1,386,862 - Pf1N1B4_1222 0.55 -2.3 1,386,862 - Pf1N1B4_1222 0.55 -1.5 1,386,862 - Pf1N1B4_1222 0.55 -1.5 1,386,862 - Pf1N1B4_1222 0.55 -1.0 1,386,864 + Pf1N1B4_1222 0.55 -1.5 1,386,962 + Pf1N1B4_1222 0.66 -1.5 1,386,962 + Pf1N1B4_1222 0.66 +0.6 1,386,962 + Pf1N1B4_1222 0.66 -0.3 1,386,962 + Pf1N1B4_1222 0.66 -1.1 1,386,962 + Pf1N1B4_1222 0.66 +0.6 1,386,962 + +2.8 1,386,962 + Pf1N1B4_1222 0.66 -0.0 1,386,962 + Pf1N1B4_1222 0.66 -0.5 1,386,962 + Pf1N1B4_1222 0.66 -1.1 1,386,962 + Pf1N1B4_1222 0.66 -1.8 1,386,962 + Pf1N1B4_1222 0.66 -0.7 1,386,962 + Pf1N1B4_1222 0.66 -0.9 1,386,962 + Pf1N1B4_1222 0.66 +1.5 1,386,962 + Pf1N1B4_1222 0.66 -0.5 1,386,963 - Pf1N1B4_1222 0.66 -1.4 1,386,963 - Pf1N1B4_1222 0.66 +1.8 1,386,963 - Pf1N1B4_1222 0.66 -0.0 1,386,963 - Pf1N1B4_1222 0.66 -0.3 1,386,963 - Pf1N1B4_1222 0.66 +3.7 1,386,963 - Pf1N1B4_1222 0.66 -1.1 1,386,963 - Pf1N1B4_1222 0.66 -0.7 1,386,963 - +0.7 1,386,963 - Pf1N1B4_1222 0.66 -1.6 1,386,963 - Pf1N1B4_1222 0.66 +0.2 1,386,963 - Pf1N1B4_1222 0.66 +0.8 1,386,963 - Pf1N1B4_1222 0.66 -1.3 1,386,963 - Pf1N1B4_1222 0.66 +0.6 1,386,963 - Pf1N1B4_1222 0.66 +1.8 1,386,963 - Pf1N1B4_1222 0.66 -1.1 1,386,963 - Pf1N1B4_1222 0.66 -1.3 1,386,963 - Pf1N1B4_1222 0.66 -2.2 1,386,963 - Pf1N1B4_1222 0.66 -0.8 1,386,963 - Pf1N1B4_1222 0.66 +3.2 1,387,162 - Pf1N1B4_1222 0.88 -2.2 1,387,164 + Pf1N1B4_1222 0.88 +0.8 1,387,164 + Pf1N1B4_1222 0.88 +2.0 1,387,164 + Pf1N1B4_1222 0.88 -1.4 1,387,164 + Pf1N1B4_1222 0.88 +2.7 1,387,165 - Pf1N1B4_1222 0.88 -2.5 1,387,165 - Pf1N1B4_1222 0.88 -1.7 1,387,165 - Pf1N1B4_1222 0.88 +1.0 1,387,165 - Pf1N1B4_1222 0.88 +2.9 1,387,165 - Pf1N1B4_1222 0.88 +0.3 1,387,165 - Pf1N1B4_1222 0.88 -0.0 1,387,165 - Pf1N1B4_1222 0.88 -1.1 1,387,253 + +0.3 1,387,266 + -2.4 1,387,266 + -2.4 1,387,266 + -0.5 1,387,441 - +2.0 1,387,530 + Pf1N1B4_1223 0.17 -2.3 1,387,686 + Pf1N1B4_1223 0.31 -1.0 1,387,686 + Pf1N1B4_1223 0.31 +0.2 1,387,686 + Pf1N1B4_1223 0.31 -1.0 1,387,686 + Pf1N1B4_1223 0.31 -1.1 1,387,686 + Pf1N1B4_1223 0.31 +1.0 1,387,686 + Pf1N1B4_1223 0.31 -0.7 1,387,686 + Pf1N1B4_1223 0.31 +1.7 1,387,686 + Pf1N1B4_1223 0.31 +2.5 1,387,687 - Pf1N1B4_1223 0.31 -0.3 1,387,687 - Pf1N1B4_1223 0.31 -0.9 1,387,687 - Pf1N1B4_1223 0.31 +1.1 1,387,687 - Pf1N1B4_1223 0.31 +2.5 1,387,687 - Pf1N1B4_1223 0.31 +1.8 1,387,687 - Pf1N1B4_1223 0.31 +0.8 1,387,687 - Pf1N1B4_1223 0.31 -1.3 1,387,722 + Pf1N1B4_1223 0.35 -0.1 1,387,722 + Pf1N1B4_1223 0.35 +1.0 1,387,722 + Pf1N1B4_1223 0.35 +1.8 1,387,722 + Pf1N1B4_1223 0.35 -0.5 1,387,722 + Pf1N1B4_1223 0.35 +0.3 1,387,723 - Pf1N1B4_1223 0.35 +2.4 1,387,723 - Pf1N1B4_1223 0.35 -1.5 1,387,723 - Pf1N1B4_1223 0.35 +1.4 1,387,723 - Pf1N1B4_1223 0.35 -1.1 1,387,783 - Pf1N1B4_1223 0.40 -2.3 1,387,783 - Pf1N1B4_1223 0.40 -1.4 1,387,861 - Pf1N1B4_1223 0.48 +0.6 1,387,881 - Pf1N1B4_1223 0.49 +2.4 1,387,971 + Pf1N1B4_1223 0.58 -1.0 1,387,972 - Pf1N1B4_1223 0.58 +2.9 1,387,972 - Pf1N1B4_1223 0.58 +0.5 1,388,019 + Pf1N1B4_1223 0.62 +2.2 1,388,019 + Pf1N1B4_1223 0.62 -2.7 1,388,115 + Pf1N1B4_1223 0.71 +0.2 1,388,116 - Pf1N1B4_1223 0.71 -1.5 1,388,116 - Pf1N1B4_1223 0.71 -1.1 1,388,116 - Pf1N1B4_1223 0.71 -1.6 1,388,128 + Pf1N1B4_1223 0.72 -1.6 1,388,336 + +1.0 1,388,336 + -1.6 1,388,337 - +0.2 1,388,337 - +0.9 1,388,374 - +1.9 1,388,660 + -0.4 1,388,660 + -1.2 1,388,660 + +3.0 1,388,660 + -1.1 1,388,660 + -1.0 1,388,660 + +0.1 1,388,661 - -0.0 1,388,661 - +2.2 1,388,661 - +2.8 1,388,661 - -0.3 1,388,661 - -1.1 1,388,661 - -0.5 1,388,661 - +0.5 1,388,684 + -2.4 1,388,684 + +0.4 1,388,684 + +0.5 1,388,684 + -1.3 1,388,684 + -0.0 1,388,685 - -2.1 1,388,685 - -0.9 1,388,705 + +0.7 1,388,705 + -1.9 1,388,705 + +1.2 1,388,705 + +0.7 1,388,705 + -1.9 1,388,705 + +0.5 1,388,705 + -0.6 1,388,705 + +0.4 1,388,706 - -1.3 1,388,706 - +2.3 1,388,706 - -2.2 1,388,706 - -0.4 1,388,706 - +0.0 1,388,706 - -1.4 1,388,706 - -1.5 1,388,706 - -1.9 1,388,706 - +1.8 1,388,706 - -0.3 1,388,706 - -1.1 1,388,706 - -0.4 1,388,706 - -1.0 1,388,706 - -1.7 1,388,706 - -0.5 1,388,706 - +0.5 1,388,706 - -0.6 1,388,706 - -1.8 1,388,706 - -2.1 1,388,706 - -1.6 1,388,706 - -1.8 1,388,706 - -0.4 1,388,706 - -2.7 1,388,706 - -0.3 1,388,706 - -2.5 1,388,710 - -1.0 1,388,815 - Pf1N1B4_1224 0.15 -0.2 1,388,847 - Pf1N1B4_1224 0.16 -2.1 1,389,012 - Pf1N1B4_1224 0.24 -2.9 1,389,063 - Pf1N1B4_1224 0.26 +0.5 1,389,128 + Pf1N1B4_1224 0.29 -0.8 1,389,128 + Pf1N1B4_1224 0.29 -2.6 1,389,128 + Pf1N1B4_1224 0.29 -1.9 1,389,129 - Pf1N1B4_1224 0.29 -2.0 1,389,139 + Pf1N1B4_1224 0.30 -1.3 1,389,139 + Pf1N1B4_1224 0.30 -0.4 1,389,139 + Pf1N1B4_1224 0.30 -0.2 1,389,139 + Pf1N1B4_1224 0.30 +0.2 1,389,140 - Pf1N1B4_1224 0.30 +0.2 1,389,140 - Pf1N1B4_1224 0.30 +2.8 1,389,140 - Pf1N1B4_1224 0.30 -0.4 1,389,140 - Pf1N1B4_1224 0.30 +2.7 1,389,140 - Pf1N1B4_1224 0.30 +2.8 1,389,345 + Pf1N1B4_1224 0.39 -0.8 1,389,345 + Pf1N1B4_1224 0.39 -1.8 1,389,345 + Pf1N1B4_1224 0.39 -2.1 1,389,345 + Pf1N1B4_1224 0.39 -0.4 1,389,345 + Pf1N1B4_1224 0.39 -2.1 1,389,346 - Pf1N1B4_1224 0.39 +0.4 1,389,346 - Pf1N1B4_1224 0.39 +1.5 1,389,346 - +1.8 1,389,346 - Pf1N1B4_1224 0.39 -1.2
Or see this region's nucleotide sequence