Experiment: KB with Gossypol-Acetic Acid Complex 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0602 and Psyr_0603 are separated by 35 nucleotides Psyr_0603 and Psyr_0604 are separated by 72 nucleotides
Psyr_0602: Psyr_0602 - Short-chain dehydrogenase/reductase SDR, at 691,538 to 692,302
_0602
Psyr_0603: Psyr_0603 - N-acylglucosamine 2-epimerase, at 692,338 to 693,552
_0603
Psyr_0604: Psyr_0604 - 1-deoxy-D-xylulose-5-phosphate synthase, at 693,625 to 695,517
_0604
Position (kb) 692
693
694 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 691.433 kb on + strand at 691.434 kb on - strand at 691.434 kb on - strand at 691.494 kb on + strand at 691.494 kb on + strand at 691.494 kb on + strand at 691.495 kb on - strand at 691.495 kb on - strand at 691.551 kb on + strand at 691.551 kb on + strand at 691.551 kb on + strand at 691.552 kb on - strand at 691.552 kb on - strand at 691.552 kb on - strand at 691.552 kb on - strand at 691.552 kb on - strand at 691.552 kb on - strand at 691.552 kb on - strand at 691.552 kb on - strand at 691.696 kb on + strand, within Psyr_0602 at 691.696 kb on + strand, within Psyr_0602 at 691.713 kb on + strand, within Psyr_0602 at 691.713 kb on + strand, within Psyr_0602 at 691.713 kb on + strand, within Psyr_0602 at 691.713 kb on + strand, within Psyr_0602 at 691.713 kb on + strand, within Psyr_0602 at 691.713 kb on + strand, within Psyr_0602 at 691.714 kb on - strand, within Psyr_0602 at 691.714 kb on - strand, within Psyr_0602 at 691.714 kb on - strand, within Psyr_0602 at 691.714 kb on - strand, within Psyr_0602 at 691.714 kb on - strand, within Psyr_0602 at 691.714 kb on - strand, within Psyr_0602 at 691.883 kb on + strand, within Psyr_0602 at 691.883 kb on + strand, within Psyr_0602 at 691.883 kb on + strand, within Psyr_0602 at 691.883 kb on + strand, within Psyr_0602 at 691.883 kb on + strand, within Psyr_0602 at 691.884 kb on - strand, within Psyr_0602 at 691.884 kb on - strand at 691.967 kb on + strand, within Psyr_0602 at 691.974 kb on - strand, within Psyr_0602 at 691.992 kb on - strand, within Psyr_0602 at 692.138 kb on + strand, within Psyr_0602 at 692.138 kb on + strand, within Psyr_0602 at 692.138 kb on + strand, within Psyr_0602 at 692.139 kb on - strand, within Psyr_0602 at 692.139 kb on - strand, within Psyr_0602 at 692.139 kb on - strand, within Psyr_0602 at 692.139 kb on - strand, within Psyr_0602 at 692.150 kb on + strand, within Psyr_0602 at 692.150 kb on + strand, within Psyr_0602 at 692.150 kb on + strand, within Psyr_0602 at 692.151 kb on - strand, within Psyr_0602 at 692.151 kb on - strand, within Psyr_0602 at 692.151 kb on - strand, within Psyr_0602 at 692.411 kb on + strand at 692.412 kb on - strand at 692.412 kb on - strand at 692.412 kb on - strand at 692.412 kb on - strand at 692.412 kb on - strand at 692.412 kb on - strand at 692.412 kb on - strand at 692.586 kb on - strand, within Psyr_0603 at 692.586 kb on - strand, within Psyr_0603 at 692.604 kb on + strand, within Psyr_0603 at 692.605 kb on - strand, within Psyr_0603 at 692.693 kb on + strand, within Psyr_0603 at 692.871 kb on + strand, within Psyr_0603 at 693.015 kb on - strand, within Psyr_0603 at 693.107 kb on + strand, within Psyr_0603 at 693.126 kb on + strand, within Psyr_0603 at 693.143 kb on + strand, within Psyr_0603 at 693.144 kb on - strand, within Psyr_0603 at 693.191 kb on + strand, within Psyr_0603 at 693.191 kb on + strand, within Psyr_0603 at 693.192 kb on - strand, within Psyr_0603 at 693.384 kb on - strand, within Psyr_0603 at 693.555 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Gossypol-Acetic Acid Complex 10 mM remove 691,433 + -0.4 691,434 - -0.7 691,434 - +0.1 691,494 + -1.0 691,494 + +0.2 691,494 + +0.5 691,495 - -0.2 691,495 - -2.1 691,551 + +0.7 691,551 + +1.8 691,551 + +0.0 691,552 - -1.6 691,552 - -1.0 691,552 - -0.6 691,552 - -0.1 691,552 - +0.6 691,552 - +0.1 691,552 - -1.3 691,552 - -0.3 691,696 + Psyr_0602 0.21 -0.7 691,696 + Psyr_0602 0.21 +1.3 691,713 + Psyr_0602 0.23 -0.1 691,713 + Psyr_0602 0.23 -1.0 691,713 + Psyr_0602 0.23 -0.9 691,713 + Psyr_0602 0.23 -0.0 691,713 + Psyr_0602 0.23 +0.1 691,713 + Psyr_0602 0.23 +1.3 691,714 - Psyr_0602 0.23 -0.7 691,714 - Psyr_0602 0.23 -0.2 691,714 - Psyr_0602 0.23 -0.9 691,714 - Psyr_0602 0.23 +0.1 691,714 - Psyr_0602 0.23 +1.3 691,714 - Psyr_0602 0.23 +0.2 691,883 + Psyr_0602 0.45 +1.4 691,883 + Psyr_0602 0.45 -0.8 691,883 + Psyr_0602 0.45 +0.8 691,883 + Psyr_0602 0.45 +0.7 691,883 + Psyr_0602 0.45 +0.2 691,884 - Psyr_0602 0.45 +0.2 691,884 - +1.0 691,967 + Psyr_0602 0.56 -0.9 691,974 - Psyr_0602 0.57 -0.1 691,992 - Psyr_0602 0.59 -1.5 692,138 + Psyr_0602 0.78 -0.2 692,138 + Psyr_0602 0.78 -1.2 692,138 + Psyr_0602 0.78 -0.8 692,139 - Psyr_0602 0.79 +1.0 692,139 - Psyr_0602 0.79 -0.0 692,139 - Psyr_0602 0.79 -0.7 692,139 - Psyr_0602 0.79 -0.5 692,150 + Psyr_0602 0.80 +0.4 692,150 + Psyr_0602 0.80 +1.7 692,150 + Psyr_0602 0.80 +0.0 692,151 - Psyr_0602 0.80 -0.2 692,151 - Psyr_0602 0.80 -0.9 692,151 - Psyr_0602 0.80 +0.1 692,411 + -0.9 692,412 - -2.4 692,412 - -1.3 692,412 - -1.9 692,412 - -1.2 692,412 - -1.7 692,412 - -0.1 692,412 - -2.9 692,586 - Psyr_0603 0.20 +0.7 692,586 - Psyr_0603 0.20 -1.9 692,604 + Psyr_0603 0.22 -0.4 692,605 - Psyr_0603 0.22 -0.8 692,693 + Psyr_0603 0.29 -1.8 692,871 + Psyr_0603 0.44 -2.3 693,015 - Psyr_0603 0.56 -1.0 693,107 + Psyr_0603 0.63 -0.9 693,126 + Psyr_0603 0.65 -2.4 693,143 + Psyr_0603 0.66 -1.3 693,144 - Psyr_0603 0.66 +0.3 693,191 + Psyr_0603 0.70 -2.4 693,191 + Psyr_0603 0.70 -1.7 693,192 - Psyr_0603 0.70 -0.9 693,384 - Psyr_0603 0.86 -1.3 693,555 + -1.2
Or see this region's nucleotide sequence