Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5982

Experiment: carbon source Sucrose 0.25mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5980 and Pf1N1B4_5981 are separated by 239 nucleotidesPf1N1B4_5981 and Pf1N1B4_5982 are separated by 140 nucleotidesPf1N1B4_5982 and Pf1N1B4_5983 are separated by 23 nucleotides Pf1N1B4_5980 - Organic hydroperoxide resistance protein, at 429,380 to 429,832 _5980 Pf1N1B4_5981 - Organic hydroperoxide resistance transcriptional regulator, at 430,072 to 430,533 _5981 Pf1N1B4_5982 - Medium-chain-fatty-acid--CoA ligase (EC 6.2.1.-), at 430,674 to 432,317 _5982 Pf1N1B4_5983 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 432,341 to 434,089 _5983 Position (kb) 430 431 432 433Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 429.684 kb on - strand, within Pf1N1B4_5980at 429.859 kb on - strandat 429.859 kb on - strandat 429.906 kb on + strandat 429.907 kb on - strandat 429.907 kb on - strandat 429.907 kb on - strandat 429.907 kb on - strandat 429.907 kb on - strandat 429.907 kb on - strandat 430.201 kb on + strand, within Pf1N1B4_5981at 430.210 kb on + strand, within Pf1N1B4_5981at 430.540 kb on - strandat 430.563 kb on + strandat 430.619 kb on + strandat 430.706 kb on + strandat 430.707 kb on - strandat 430.707 kb on - strandat 430.707 kb on - strandat 430.707 kb on - strandat 430.707 kb on - strandat 430.707 kb on - strandat 430.710 kb on + strandat 430.731 kb on - strandat 431.108 kb on - strand, within Pf1N1B4_5982at 431.288 kb on - strand, within Pf1N1B4_5982at 431.288 kb on - strand, within Pf1N1B4_5982at 431.345 kb on + strand, within Pf1N1B4_5982at 431.372 kb on + strand, within Pf1N1B4_5982at 431.376 kb on + strand, within Pf1N1B4_5982at 431.376 kb on + strand, within Pf1N1B4_5982at 431.377 kb on - strand, within Pf1N1B4_5982at 431.377 kb on - strand, within Pf1N1B4_5982at 431.377 kb on - strand, within Pf1N1B4_5982at 431.626 kb on + strandat 431.626 kb on + strand, within Pf1N1B4_5982at 431.627 kb on - strand, within Pf1N1B4_5982at 431.627 kb on - strand, within Pf1N1B4_5982at 431.627 kb on - strand, within Pf1N1B4_5982at 431.627 kb on - strand, within Pf1N1B4_5982at 431.723 kb on - strand, within Pf1N1B4_5982at 431.732 kb on + strandat 431.803 kb on + strand, within Pf1N1B4_5982at 431.871 kb on + strand, within Pf1N1B4_5982at 431.872 kb on - strand, within Pf1N1B4_5982at 431.872 kb on - strand, within Pf1N1B4_5982at 431.872 kb on - strand, within Pf1N1B4_5982at 431.872 kb on - strand, within Pf1N1B4_5982at 431.894 kb on - strand, within Pf1N1B4_5982at 432.181 kb on + strandat 432.182 kb on - strandat 432.277 kb on + strandat 432.301 kb on - strandat 432.523 kb on - strand, within Pf1N1B4_5983at 432.523 kb on - strand, within Pf1N1B4_5983at 432.523 kb on - strand, within Pf1N1B4_5983at 432.523 kb on - strand, within Pf1N1B4_5983at 432.529 kb on - strandat 432.798 kb on + strandat 432.798 kb on + strand, within Pf1N1B4_5983at 432.798 kb on + strandat 432.824 kb on + strand, within Pf1N1B4_5983at 432.990 kb on + strand, within Pf1N1B4_5983at 432.991 kb on - strand, within Pf1N1B4_5983at 433.080 kb on - strand, within Pf1N1B4_5983at 433.236 kb on + strandat 433.236 kb on + strand, within Pf1N1B4_5983

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM
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429,684 - Pf1N1B4_5980 0.67 -0.9
429,859 - -1.1
429,859 - +2.0
429,906 + -1.9
429,907 - -0.9
429,907 - +2.1
429,907 - +3.3
429,907 - +1.8
429,907 - +1.1
429,907 - -1.0
430,201 + Pf1N1B4_5981 0.28 -2.4
430,210 + Pf1N1B4_5981 0.30 -1.1
430,540 - +0.5
430,563 + +0.8
430,619 + +2.0
430,706 + +0.5
430,707 - -3.3
430,707 - +2.0
430,707 - -1.2
430,707 - -1.8
430,707 - -1.5
430,707 - -0.4
430,710 + +2.4
430,731 - +1.8
431,108 - Pf1N1B4_5982 0.26 -2.2
431,288 - Pf1N1B4_5982 0.37 +0.2
431,288 - Pf1N1B4_5982 0.37 -1.9
431,345 + Pf1N1B4_5982 0.41 +0.8
431,372 + Pf1N1B4_5982 0.42 -2.6
431,376 + Pf1N1B4_5982 0.43 -1.8
431,376 + Pf1N1B4_5982 0.43 -1.1
431,377 - Pf1N1B4_5982 0.43 -1.4
431,377 - Pf1N1B4_5982 0.43 -0.5
431,377 - Pf1N1B4_5982 0.43 -0.3
431,626 + -1.2
431,626 + Pf1N1B4_5982 0.58 -1.8
431,627 - Pf1N1B4_5982 0.58 -0.8
431,627 - Pf1N1B4_5982 0.58 +1.0
431,627 - Pf1N1B4_5982 0.58 -3.2
431,627 - Pf1N1B4_5982 0.58 -2.5
431,723 - Pf1N1B4_5982 0.64 -2.6
431,732 + +0.6
431,803 + Pf1N1B4_5982 0.69 -0.5
431,871 + Pf1N1B4_5982 0.73 -1.4
431,872 - Pf1N1B4_5982 0.73 -1.4
431,872 - Pf1N1B4_5982 0.73 -2.4
431,872 - Pf1N1B4_5982 0.73 -2.0
431,872 - Pf1N1B4_5982 0.73 -2.4
431,894 - Pf1N1B4_5982 0.74 -0.7
432,181 + -1.2
432,182 - -2.4
432,277 + +0.2
432,301 - +1.4
432,523 - Pf1N1B4_5983 0.10 +1.3
432,523 - Pf1N1B4_5983 0.10 +2.6
432,523 - Pf1N1B4_5983 0.10 -2.3
432,523 - Pf1N1B4_5983 0.10 +1.3
432,529 - -1.8
432,798 + -0.4
432,798 + Pf1N1B4_5983 0.26 -2.2
432,798 + -1.5
432,824 + Pf1N1B4_5983 0.28 +2.6
432,990 + Pf1N1B4_5983 0.37 -2.2
432,991 - Pf1N1B4_5983 0.37 +0.2
433,080 - Pf1N1B4_5983 0.42 +0.1
433,236 + +0.4
433,236 + Pf1N1B4_5983 0.51 -0.1

Or see this region's nucleotide sequence