Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4784

Experiment: carbon source Sucrose 0.25mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4783 and Pf1N1B4_4784 are separated by 254 nucleotidesPf1N1B4_4784 and Pf1N1B4_4785 are separated by 16 nucleotidesPf1N1B4_4785 and Pf1N1B4_4786 are separated by 12 nucleotides Pf1N1B4_4783 - Transcriptional regulator, AraC family, at 5,284,560 to 5,285,576 _4783 Pf1N1B4_4784 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases, at 5,285,831 to 5,287,495 _4784 Pf1N1B4_4785 - Probable acyl-CoA dehydrogenase (EC 1.3.99.3), at 5,287,512 to 5,288,273 _4785 Pf1N1B4_4786 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 5,288,286 to 5,289,479 _4786 Position (kb) 5285 5286 5287 5288Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5284.974 kb on + strand, within Pf1N1B4_4783at 5284.974 kb on + strand, within Pf1N1B4_4783at 5284.975 kb on - strandat 5284.984 kb on - strand, within Pf1N1B4_4783at 5285.385 kb on + strand, within Pf1N1B4_4783at 5285.385 kb on + strand, within Pf1N1B4_4783at 5285.385 kb on + strand, within Pf1N1B4_4783at 5285.502 kb on + strandat 5285.502 kb on + strandat 5285.531 kb on + strandat 5285.574 kb on + strandat 5285.574 kb on + strandat 5285.574 kb on + strandat 5285.697 kb on + strandat 5285.697 kb on + strandat 5285.698 kb on - strandat 5285.698 kb on - strandat 5285.698 kb on - strandat 5285.702 kb on - strandat 5285.702 kb on - strandat 5285.788 kb on + strandat 5285.788 kb on + strandat 5285.789 kb on - strandat 5285.789 kb on - strandat 5285.804 kb on + strandat 5285.841 kb on - strandat 5285.841 kb on - strandat 5285.867 kb on + strandat 5285.867 kb on + strandat 5286.488 kb on + strandat 5286.566 kb on + strand, within Pf1N1B4_4784at 5286.566 kb on + strand, within Pf1N1B4_4784at 5286.567 kb on - strand, within Pf1N1B4_4784at 5286.614 kb on + strand, within Pf1N1B4_4784at 5286.615 kb on - strand, within Pf1N1B4_4784at 5286.662 kb on + strand, within Pf1N1B4_4784at 5286.662 kb on + strand, within Pf1N1B4_4784at 5286.963 kb on - strand, within Pf1N1B4_4784at 5287.076 kb on + strand, within Pf1N1B4_4784at 5287.076 kb on + strand, within Pf1N1B4_4784at 5287.077 kb on - strand, within Pf1N1B4_4784at 5287.079 kb on + strand, within Pf1N1B4_4784at 5287.432 kb on + strandat 5288.182 kb on - strand, within Pf1N1B4_4785at 5288.202 kb on - strandat 5288.202 kb on - strandat 5288.202 kb on - strandat 5288.202 kb on - strandat 5288.206 kb on + strandat 5288.206 kb on + strandat 5288.271 kb on + strandat 5288.272 kb on - strandat 5288.272 kb on - strandat 5288.281 kb on + strandat 5288.282 kb on - strandat 5288.282 kb on - strandat 5288.282 kb on - strandat 5288.312 kb on + strandat 5288.313 kb on - strandat 5288.313 kb on - strandat 5288.313 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM
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5,284,974 + Pf1N1B4_4783 0.41 +0.4
5,284,974 + Pf1N1B4_4783 0.41 -1.7
5,284,975 - -0.4
5,284,984 - Pf1N1B4_4783 0.42 -2.6
5,285,385 + Pf1N1B4_4783 0.81 +0.1
5,285,385 + Pf1N1B4_4783 0.81 -0.8
5,285,385 + Pf1N1B4_4783 0.81 -1.9
5,285,502 + -2.2
5,285,502 + +0.1
5,285,531 + +0.4
5,285,574 + -1.6
5,285,574 + -0.7
5,285,574 + +1.1
5,285,697 + +1.2
5,285,697 + -0.4
5,285,698 - +2.2
5,285,698 - +0.1
5,285,698 - +0.7
5,285,702 - -0.1
5,285,702 - +3.4
5,285,788 + -2.0
5,285,788 + -0.8
5,285,789 - +0.4
5,285,789 - -2.4
5,285,804 + -0.8
5,285,841 - +2.0
5,285,841 - -0.9
5,285,867 + -2.7
5,285,867 + +0.0
5,286,488 + +1.8
5,286,566 + Pf1N1B4_4784 0.44 -1.9
5,286,566 + Pf1N1B4_4784 0.44 -2.3
5,286,567 - Pf1N1B4_4784 0.44 +0.5
5,286,614 + Pf1N1B4_4784 0.47 +1.9
5,286,615 - Pf1N1B4_4784 0.47 +1.7
5,286,662 + Pf1N1B4_4784 0.50 +1.3
5,286,662 + Pf1N1B4_4784 0.50 +2.1
5,286,963 - Pf1N1B4_4784 0.68 +2.9
5,287,076 + Pf1N1B4_4784 0.75 -1.2
5,287,076 + Pf1N1B4_4784 0.75 +0.9
5,287,077 - Pf1N1B4_4784 0.75 -1.5
5,287,079 + Pf1N1B4_4784 0.75 +1.7
5,287,432 + -2.3
5,288,182 - Pf1N1B4_4785 0.88 -0.8
5,288,202 - -0.9
5,288,202 - +0.9
5,288,202 - -2.7
5,288,202 - +1.3
5,288,206 + +0.2
5,288,206 + -1.2
5,288,271 + -0.5
5,288,272 - +0.2
5,288,272 - -1.9
5,288,281 + -0.2
5,288,282 - +0.6
5,288,282 - -0.6
5,288,282 - -1.9
5,288,312 + +1.8
5,288,313 - -0.8
5,288,313 - +2.5
5,288,313 - +0.6

Or see this region's nucleotide sequence