Experiment: carbon source Sucrose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4612 and Pf1N1B4_4613 overlap by 4 nucleotides Pf1N1B4_4613 and Pf1N1B4_4614 are separated by 10 nucleotides
Pf1N1B4_4612 - FIG00954117: hypothetical protein, at 5,081,804 to 5,082,064
_4612
Pf1N1B4_4613 - FIG00953934: hypothetical protein, at 5,082,061 to 5,082,891
_4613
Pf1N1B4_4614 - Membrane alanine aminopeptidase N (EC 3.4.11.2), at 5,082,902 to 5,085,559
_4614
Position (kb)
5082
5083
5084
5085
5086 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5081.987 kb on + strand at 5081.988 kb on - strand, within Pf1N1B4_4612 at 5082.197 kb on - strand, within Pf1N1B4_4613 at 5082.329 kb on - strand, within Pf1N1B4_4613 at 5082.329 kb on - strand, within Pf1N1B4_4613 at 5082.578 kb on + strand, within Pf1N1B4_4613 at 5082.855 kb on + strand at 5082.859 kb on + strand at 5082.859 kb on + strand at 5082.860 kb on - strand at 5082.902 kb on - strand at 5083.140 kb on - strand at 5083.247 kb on + strand, within Pf1N1B4_4614 at 5083.248 kb on - strand, within Pf1N1B4_4614 at 5083.308 kb on - strand at 5083.337 kb on - strand, within Pf1N1B4_4614 at 5083.345 kb on + strand, within Pf1N1B4_4614 at 5083.559 kb on + strand, within Pf1N1B4_4614 at 5083.560 kb on - strand at 5083.791 kb on + strand at 5083.792 kb on - strand, within Pf1N1B4_4614 at 5084.139 kb on - strand, within Pf1N1B4_4614 at 5084.945 kb on + strand at 5085.654 kb on + strand at 5085.655 kb on - strand at 5085.671 kb on - strand at 5085.727 kb on + strand at 5085.727 kb on + strand at 5085.728 kb on - strand at 5085.728 kb on - strand at 5085.732 kb on - strand at 5086.060 kb on - strand at 5086.535 kb on - strand at 5086.539 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM remove 5,081,987 + -1.1 5,081,988 - Pf1N1B4_4612 0.70 +2.0 5,082,197 - Pf1N1B4_4613 0.16 -0.6 5,082,329 - Pf1N1B4_4613 0.32 +0.1 5,082,329 - Pf1N1B4_4613 0.32 -0.3 5,082,578 + Pf1N1B4_4613 0.62 -0.3 5,082,855 + -1.1 5,082,859 + +0.9 5,082,859 + +2.3 5,082,860 - +2.1 5,082,902 - -0.9 5,083,140 - -1.5 5,083,247 + Pf1N1B4_4614 0.13 -0.1 5,083,248 - Pf1N1B4_4614 0.13 -2.2 5,083,308 - -0.9 5,083,337 - Pf1N1B4_4614 0.16 -3.7 5,083,345 + Pf1N1B4_4614 0.17 -0.5 5,083,559 + Pf1N1B4_4614 0.25 -1.7 5,083,560 - +1.0 5,083,791 + -0.8 5,083,792 - Pf1N1B4_4614 0.33 -0.5 5,084,139 - Pf1N1B4_4614 0.47 -2.3 5,084,945 + -0.6 5,085,654 + +3.6 5,085,655 - +1.9 5,085,671 - -1.0 5,085,727 + +1.1 5,085,727 + -2.0 5,085,728 - -1.3 5,085,728 - -0.3 5,085,732 - +0.7 5,086,060 - +1.1 5,086,535 - +1.6 5,086,539 - -1.8
Or see this region's nucleotide sequence