Experiment: carbon source Sucrose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4449 and Pf1N1B4_4450 are separated by 179 nucleotides Pf1N1B4_4450 and Pf1N1B4_4451 are separated by 133 nucleotides
Pf1N1B4_4449 - Permeases of the major facilitator superfamily, at 4,908,027 to 4,909,649
_4449
Pf1N1B4_4450 - Choline dehydrogenase (EC 1.1.99.1), at 4,909,829 to 4,911,478
_4450
Pf1N1B4_4451 - Transcriptional regulator, LysR family, at 4,911,612 to 4,912,496
_4451
Position (kb)
4909
4910
4911
4912 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4908.838 kb on + strand, within Pf1N1B4_4449 at 4908.839 kb on - strand, within Pf1N1B4_4449 at 4908.839 kb on - strand, within Pf1N1B4_4449 at 4908.839 kb on - strand, within Pf1N1B4_4449 at 4909.097 kb on - strand, within Pf1N1B4_4449 at 4909.207 kb on + strand, within Pf1N1B4_4449 at 4909.207 kb on + strand at 4909.208 kb on - strand, within Pf1N1B4_4449 at 4909.304 kb on - strand, within Pf1N1B4_4449 at 4909.369 kb on + strand, within Pf1N1B4_4449 at 4909.528 kb on + strand at 4909.729 kb on - strand at 4909.729 kb on - strand at 4909.740 kb on + strand at 4909.740 kb on + strand at 4909.740 kb on + strand at 4909.741 kb on - strand at 4909.741 kb on - strand at 4909.754 kb on + strand at 4909.755 kb on - strand at 4909.830 kb on + strand at 4909.830 kb on + strand at 4909.830 kb on + strand at 4909.936 kb on + strand at 4909.937 kb on - strand at 4910.140 kb on - strand, within Pf1N1B4_4450 at 4910.213 kb on + strand, within Pf1N1B4_4450 at 4910.241 kb on + strand, within Pf1N1B4_4450 at 4910.241 kb on + strand, within Pf1N1B4_4450 at 4910.242 kb on - strand, within Pf1N1B4_4450 at 4910.242 kb on - strand at 4910.397 kb on + strand, within Pf1N1B4_4450 at 4910.488 kb on - strand, within Pf1N1B4_4450 at 4910.493 kb on + strand, within Pf1N1B4_4450 at 4910.804 kb on + strand, within Pf1N1B4_4450 at 4911.160 kb on - strand, within Pf1N1B4_4450 at 4911.160 kb on - strand, within Pf1N1B4_4450 at 4911.183 kb on + strand at 4911.401 kb on + strand at 4911.450 kb on + strand at 4911.451 kb on - strand at 4911.451 kb on - strand at 4912.104 kb on + strand, within Pf1N1B4_4451 at 4912.104 kb on + strand, within Pf1N1B4_4451 at 4912.104 kb on + strand, within Pf1N1B4_4451 at 4912.119 kb on + strand, within Pf1N1B4_4451 at 4912.120 kb on - strand at 4912.326 kb on + strand, within Pf1N1B4_4451 at 4912.326 kb on + strand, within Pf1N1B4_4451 at 4912.326 kb on + strand, within Pf1N1B4_4451 at 4912.327 kb on - strand, within Pf1N1B4_4451 at 4912.327 kb on - strand, within Pf1N1B4_4451 at 4912.400 kb on + strand, within Pf1N1B4_4451
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM remove 4,908,838 + Pf1N1B4_4449 0.50 -0.9 4,908,839 - Pf1N1B4_4449 0.50 -0.7 4,908,839 - Pf1N1B4_4449 0.50 -1.0 4,908,839 - Pf1N1B4_4449 0.50 +0.0 4,909,097 - Pf1N1B4_4449 0.66 -0.9 4,909,207 + Pf1N1B4_4449 0.73 -2.3 4,909,207 + -0.0 4,909,208 - Pf1N1B4_4449 0.73 +1.3 4,909,304 - Pf1N1B4_4449 0.79 +0.5 4,909,369 + Pf1N1B4_4449 0.83 +0.3 4,909,528 + +0.6 4,909,729 - -1.0 4,909,729 - -1.9 4,909,740 + +1.6 4,909,740 + -1.5 4,909,740 + +1.8 4,909,741 - +1.7 4,909,741 - -2.0 4,909,754 + +1.5 4,909,755 - +2.1 4,909,830 + +1.6 4,909,830 + -1.7 4,909,830 + -1.3 4,909,936 + -1.7 4,909,937 - -2.1 4,910,140 - Pf1N1B4_4450 0.19 -0.8 4,910,213 + Pf1N1B4_4450 0.23 -1.8 4,910,241 + Pf1N1B4_4450 0.25 -0.6 4,910,241 + Pf1N1B4_4450 0.25 -2.3 4,910,242 - Pf1N1B4_4450 0.25 +1.3 4,910,242 - -0.5 4,910,397 + Pf1N1B4_4450 0.34 -0.0 4,910,488 - Pf1N1B4_4450 0.40 -1.7 4,910,493 + Pf1N1B4_4450 0.40 -0.5 4,910,804 + Pf1N1B4_4450 0.59 +0.1 4,911,160 - Pf1N1B4_4450 0.81 -1.4 4,911,160 - Pf1N1B4_4450 0.81 -1.0 4,911,183 + +0.7 4,911,401 + -0.4 4,911,450 + +2.4 4,911,451 - +0.5 4,911,451 - -0.9 4,912,104 + Pf1N1B4_4451 0.56 +0.4 4,912,104 + Pf1N1B4_4451 0.56 +0.3 4,912,104 + Pf1N1B4_4451 0.56 -2.0 4,912,119 + Pf1N1B4_4451 0.57 -2.1 4,912,120 - +3.6 4,912,326 + Pf1N1B4_4451 0.81 -1.7 4,912,326 + Pf1N1B4_4451 0.81 -0.2 4,912,326 + Pf1N1B4_4451 0.81 -1.3 4,912,327 - Pf1N1B4_4451 0.81 +1.2 4,912,327 - Pf1N1B4_4451 0.81 +1.0 4,912,400 + Pf1N1B4_4451 0.89 +0.6
Or see this region's nucleotide sequence