Experiment: carbon source Sucrose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4106 and Pf1N1B4_4107 are separated by 240 nucleotides Pf1N1B4_4107 and Pf1N1B4_4108 are separated by 15 nucleotides Pf1N1B4_4108 and Pf1N1B4_4109 are separated by 133 nucleotides Pf1N1B4_4109 and Pf1N1B4_4110 are separated by 130 nucleotides
Pf1N1B4_4106 - Mesenchymal stem cell protein DSCD75, at 4,553,460 to 4,554,032
_4106
Pf1N1B4_4107 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 4,554,273 to 4,554,905
_4107
Pf1N1B4_4108 - Hydrolase, alpha/beta fold family, at 4,554,921 to 4,555,718
_4108
Pf1N1B4_4109 - Cytochrome c2, at 4,555,852 to 4,556,241
_4109
Pf1N1B4_4110 - hypothetical protein, at 4,556,372 to 4,556,707
_4110
Position (kb)
4554
4555
4556 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4554.053 kb on - strand at 4554.053 kb on - strand at 4554.053 kb on - strand at 4554.057 kb on - strand at 4554.057 kb on - strand at 4554.091 kb on - strand at 4554.420 kb on + strand, within Pf1N1B4_4107 at 4554.738 kb on + strand, within Pf1N1B4_4107 at 4554.738 kb on + strand, within Pf1N1B4_4107 at 4554.738 kb on + strand, within Pf1N1B4_4107 at 4554.903 kb on + strand at 4554.935 kb on + strand at 4554.935 kb on + strand at 4554.974 kb on + strand at 4554.986 kb on + strand at 4554.986 kb on + strand at 4554.986 kb on + strand at 4555.385 kb on - strand, within Pf1N1B4_4108 at 4555.385 kb on - strand, within Pf1N1B4_4108 at 4555.450 kb on + strand, within Pf1N1B4_4108 at 4555.451 kb on - strand, within Pf1N1B4_4108 at 4555.451 kb on - strand, within Pf1N1B4_4108 at 4555.451 kb on - strand at 4555.451 kb on - strand, within Pf1N1B4_4108 at 4555.697 kb on - strand at 4555.761 kb on + strand at 4555.761 kb on + strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.762 kb on - strand at 4555.767 kb on - strand at 4555.767 kb on - strand at 4555.786 kb on - strand at 4555.952 kb on + strand, within Pf1N1B4_4109 at 4555.952 kb on + strand, within Pf1N1B4_4109 at 4555.953 kb on - strand, within Pf1N1B4_4109 at 4555.953 kb on - strand at 4555.953 kb on - strand at 4555.962 kb on + strand, within Pf1N1B4_4109 at 4555.963 kb on - strand, within Pf1N1B4_4109 at 4555.985 kb on + strand, within Pf1N1B4_4109 at 4555.986 kb on - strand, within Pf1N1B4_4109 at 4556.062 kb on + strand at 4556.063 kb on - strand, within Pf1N1B4_4109 at 4556.068 kb on + strand, within Pf1N1B4_4109 at 4556.119 kb on - strand, within Pf1N1B4_4109 at 4556.125 kb on + strand, within Pf1N1B4_4109 at 4556.147 kb on + strand, within Pf1N1B4_4109 at 4556.216 kb on - strand at 4556.251 kb on + strand at 4556.378 kb on + strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.379 kb on - strand at 4556.474 kb on + strand, within Pf1N1B4_4110 at 4556.474 kb on + strand, within Pf1N1B4_4110 at 4556.475 kb on - strand, within Pf1N1B4_4110 at 4556.475 kb on - strand, within Pf1N1B4_4110
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM remove 4,554,053 - +0.4 4,554,053 - +1.4 4,554,053 - -1.9 4,554,057 - +1.4 4,554,057 - -2.0 4,554,091 - +1.4 4,554,420 + Pf1N1B4_4107 0.23 +0.7 4,554,738 + Pf1N1B4_4107 0.73 -2.2 4,554,738 + Pf1N1B4_4107 0.73 +1.1 4,554,738 + Pf1N1B4_4107 0.73 +2.4 4,554,903 + -1.6 4,554,935 + -1.9 4,554,935 + -1.2 4,554,974 + -0.5 4,554,986 + -1.6 4,554,986 + +0.9 4,554,986 + +2.0 4,555,385 - Pf1N1B4_4108 0.58 -1.5 4,555,385 - Pf1N1B4_4108 0.58 -3.3 4,555,450 + Pf1N1B4_4108 0.66 -0.9 4,555,451 - Pf1N1B4_4108 0.66 +0.1 4,555,451 - Pf1N1B4_4108 0.66 -0.7 4,555,451 - +0.3 4,555,451 - Pf1N1B4_4108 0.66 -1.6 4,555,697 - -0.9 4,555,761 + -2.3 4,555,761 + -0.5 4,555,762 - +1.1 4,555,762 - -0.7 4,555,762 - -1.1 4,555,762 - +0.4 4,555,762 - -1.3 4,555,762 - +1.5 4,555,762 - -1.4 4,555,762 - +0.8 4,555,762 - -1.7 4,555,767 - +0.4 4,555,767 - -0.1 4,555,786 - -0.7 4,555,952 + Pf1N1B4_4109 0.26 +2.1 4,555,952 + Pf1N1B4_4109 0.26 +1.7 4,555,953 - Pf1N1B4_4109 0.26 +1.2 4,555,953 - -0.3 4,555,953 - -0.1 4,555,962 + Pf1N1B4_4109 0.28 -0.9 4,555,963 - Pf1N1B4_4109 0.28 -2.1 4,555,985 + Pf1N1B4_4109 0.34 +1.1 4,555,986 - Pf1N1B4_4109 0.34 +0.2 4,556,062 + +1.8 4,556,063 - Pf1N1B4_4109 0.54 -0.1 4,556,068 + Pf1N1B4_4109 0.55 +2.6 4,556,119 - Pf1N1B4_4109 0.68 -0.5 4,556,125 + Pf1N1B4_4109 0.70 -1.6 4,556,147 + Pf1N1B4_4109 0.76 +0.9 4,556,216 - -1.4 4,556,251 + -1.8 4,556,378 + +0.3 4,556,379 - -1.8 4,556,379 - -1.7 4,556,379 - +0.6 4,556,379 - +1.7 4,556,379 - -1.8 4,556,474 + Pf1N1B4_4110 0.30 -1.6 4,556,474 + Pf1N1B4_4110 0.30 +1.6 4,556,475 - Pf1N1B4_4110 0.31 +0.5 4,556,475 - Pf1N1B4_4110 0.31 +2.0
Or see this region's nucleotide sequence