Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4108

Experiment: carbon source Sucrose 0.25mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4106 and Pf1N1B4_4107 are separated by 240 nucleotidesPf1N1B4_4107 and Pf1N1B4_4108 are separated by 15 nucleotidesPf1N1B4_4108 and Pf1N1B4_4109 are separated by 133 nucleotidesPf1N1B4_4109 and Pf1N1B4_4110 are separated by 130 nucleotides Pf1N1B4_4106 - Mesenchymal stem cell protein DSCD75, at 4,553,460 to 4,554,032 _4106 Pf1N1B4_4107 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 4,554,273 to 4,554,905 _4107 Pf1N1B4_4108 - Hydrolase, alpha/beta fold family, at 4,554,921 to 4,555,718 _4108 Pf1N1B4_4109 - Cytochrome c2, at 4,555,852 to 4,556,241 _4109 Pf1N1B4_4110 - hypothetical protein, at 4,556,372 to 4,556,707 _4110 Position (kb) 4554 4555 4556Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4554.053 kb on - strandat 4554.053 kb on - strandat 4554.053 kb on - strandat 4554.057 kb on - strandat 4554.057 kb on - strandat 4554.091 kb on - strandat 4554.420 kb on + strand, within Pf1N1B4_4107at 4554.738 kb on + strand, within Pf1N1B4_4107at 4554.738 kb on + strand, within Pf1N1B4_4107at 4554.738 kb on + strand, within Pf1N1B4_4107at 4554.903 kb on + strandat 4554.935 kb on + strandat 4554.935 kb on + strandat 4554.974 kb on + strandat 4554.986 kb on + strandat 4554.986 kb on + strandat 4554.986 kb on + strandat 4555.385 kb on - strand, within Pf1N1B4_4108at 4555.385 kb on - strand, within Pf1N1B4_4108at 4555.450 kb on + strand, within Pf1N1B4_4108at 4555.451 kb on - strand, within Pf1N1B4_4108at 4555.451 kb on - strand, within Pf1N1B4_4108at 4555.451 kb on - strandat 4555.451 kb on - strand, within Pf1N1B4_4108at 4555.697 kb on - strandat 4555.761 kb on + strandat 4555.761 kb on + strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.762 kb on - strandat 4555.767 kb on - strandat 4555.767 kb on - strandat 4555.786 kb on - strandat 4555.952 kb on + strand, within Pf1N1B4_4109at 4555.952 kb on + strand, within Pf1N1B4_4109at 4555.953 kb on - strand, within Pf1N1B4_4109at 4555.953 kb on - strandat 4555.953 kb on - strandat 4555.962 kb on + strand, within Pf1N1B4_4109at 4555.963 kb on - strand, within Pf1N1B4_4109at 4555.985 kb on + strand, within Pf1N1B4_4109at 4555.986 kb on - strand, within Pf1N1B4_4109at 4556.062 kb on + strandat 4556.063 kb on - strand, within Pf1N1B4_4109at 4556.068 kb on + strand, within Pf1N1B4_4109at 4556.119 kb on - strand, within Pf1N1B4_4109at 4556.125 kb on + strand, within Pf1N1B4_4109at 4556.147 kb on + strand, within Pf1N1B4_4109at 4556.216 kb on - strandat 4556.251 kb on + strandat 4556.378 kb on + strandat 4556.379 kb on - strandat 4556.379 kb on - strandat 4556.379 kb on - strandat 4556.379 kb on - strandat 4556.379 kb on - strandat 4556.474 kb on + strand, within Pf1N1B4_4110at 4556.474 kb on + strand, within Pf1N1B4_4110at 4556.475 kb on - strand, within Pf1N1B4_4110at 4556.475 kb on - strand, within Pf1N1B4_4110

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM
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4,554,053 - +0.4
4,554,053 - +1.4
4,554,053 - -1.9
4,554,057 - +1.4
4,554,057 - -2.0
4,554,091 - +1.4
4,554,420 + Pf1N1B4_4107 0.23 +0.7
4,554,738 + Pf1N1B4_4107 0.73 -2.2
4,554,738 + Pf1N1B4_4107 0.73 +1.1
4,554,738 + Pf1N1B4_4107 0.73 +2.4
4,554,903 + -1.6
4,554,935 + -1.9
4,554,935 + -1.2
4,554,974 + -0.5
4,554,986 + -1.6
4,554,986 + +0.9
4,554,986 + +2.0
4,555,385 - Pf1N1B4_4108 0.58 -1.5
4,555,385 - Pf1N1B4_4108 0.58 -3.3
4,555,450 + Pf1N1B4_4108 0.66 -0.9
4,555,451 - Pf1N1B4_4108 0.66 +0.1
4,555,451 - Pf1N1B4_4108 0.66 -0.7
4,555,451 - +0.3
4,555,451 - Pf1N1B4_4108 0.66 -1.6
4,555,697 - -0.9
4,555,761 + -2.3
4,555,761 + -0.5
4,555,762 - +1.1
4,555,762 - -0.7
4,555,762 - -1.1
4,555,762 - +0.4
4,555,762 - -1.3
4,555,762 - +1.5
4,555,762 - -1.4
4,555,762 - +0.8
4,555,762 - -1.7
4,555,767 - +0.4
4,555,767 - -0.1
4,555,786 - -0.7
4,555,952 + Pf1N1B4_4109 0.26 +2.1
4,555,952 + Pf1N1B4_4109 0.26 +1.7
4,555,953 - Pf1N1B4_4109 0.26 +1.2
4,555,953 - -0.3
4,555,953 - -0.1
4,555,962 + Pf1N1B4_4109 0.28 -0.9
4,555,963 - Pf1N1B4_4109 0.28 -2.1
4,555,985 + Pf1N1B4_4109 0.34 +1.1
4,555,986 - Pf1N1B4_4109 0.34 +0.2
4,556,062 + +1.8
4,556,063 - Pf1N1B4_4109 0.54 -0.1
4,556,068 + Pf1N1B4_4109 0.55 +2.6
4,556,119 - Pf1N1B4_4109 0.68 -0.5
4,556,125 + Pf1N1B4_4109 0.70 -1.6
4,556,147 + Pf1N1B4_4109 0.76 +0.9
4,556,216 - -1.4
4,556,251 + -1.8
4,556,378 + +0.3
4,556,379 - -1.8
4,556,379 - -1.7
4,556,379 - +0.6
4,556,379 - +1.7
4,556,379 - -1.8
4,556,474 + Pf1N1B4_4110 0.30 -1.6
4,556,474 + Pf1N1B4_4110 0.30 +1.6
4,556,475 - Pf1N1B4_4110 0.31 +0.5
4,556,475 - Pf1N1B4_4110 0.31 +2.0

Or see this region's nucleotide sequence