Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_388

Experiment: carbon source Sucrose 0.25mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_386 and Pf1N1B4_387 overlap by 24 nucleotidesPf1N1B4_387 and Pf1N1B4_388 are separated by 120 nucleotidesPf1N1B4_388 and Pf1N1B4_389 overlap by 4 nucleotidesPf1N1B4_389 and Pf1N1B4_390 are separated by 9 nucleotides Pf1N1B4_386 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 455,166 to 456,605 _386 Pf1N1B4_387 - hypothetical protein, at 456,582 to 456,695 _387 Pf1N1B4_388 - OmpA family protein, at 456,816 to 457,628 _388 Pf1N1B4_389 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 457,625 to 457,981 _389 Pf1N1B4_390 - Extracellular solute-binding protein, family 3, at 457,991 to 458,827 _390 Position (kb) 456 457 458Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 455.844 kb on - strand, within Pf1N1B4_386at 455.844 kb on - strand, within Pf1N1B4_386at 455.989 kb on + strand, within Pf1N1B4_386at 455.992 kb on + strand, within Pf1N1B4_386at 455.992 kb on + strand, within Pf1N1B4_386at 455.992 kb on + strand, within Pf1N1B4_386at 455.992 kb on + strandat 455.992 kb on + strand, within Pf1N1B4_386at 455.992 kb on + strand, within Pf1N1B4_386at 455.992 kb on + strand, within Pf1N1B4_386at 455.992 kb on + strand, within Pf1N1B4_386at 455.992 kb on + strand, within Pf1N1B4_386at 455.992 kb on + strand, within Pf1N1B4_386at 456.036 kb on + strand, within Pf1N1B4_386at 456.172 kb on + strand, within Pf1N1B4_386at 456.172 kb on + strand, within Pf1N1B4_386at 456.172 kb on + strand, within Pf1N1B4_386at 456.172 kb on + strandat 456.172 kb on + strand, within Pf1N1B4_386at 456.172 kb on + strand, within Pf1N1B4_386at 456.172 kb on + strand, within Pf1N1B4_386at 456.173 kb on - strand, within Pf1N1B4_386at 456.173 kb on - strand, within Pf1N1B4_386at 456.173 kb on - strand, within Pf1N1B4_386at 456.173 kb on - strand, within Pf1N1B4_386at 456.173 kb on - strand, within Pf1N1B4_386at 456.245 kb on - strand, within Pf1N1B4_386at 456.245 kb on - strand, within Pf1N1B4_386at 456.245 kb on - strand, within Pf1N1B4_386at 456.245 kb on - strand, within Pf1N1B4_386at 456.403 kb on + strand, within Pf1N1B4_386at 456.403 kb on + strand, within Pf1N1B4_386at 456.403 kb on + strand, within Pf1N1B4_386at 456.403 kb on + strand, within Pf1N1B4_386at 456.403 kb on + strand, within Pf1N1B4_386at 456.403 kb on + strand, within Pf1N1B4_386at 456.404 kb on - strand, within Pf1N1B4_386at 456.404 kb on - strand, within Pf1N1B4_386at 456.538 kb on + strandat 456.538 kb on + strandat 456.539 kb on - strandat 456.539 kb on - strandat 456.578 kb on + strandat 456.578 kb on + strandat 456.578 kb on + strandat 456.578 kb on + strandat 456.579 kb on - strandat 456.579 kb on - strandat 456.579 kb on - strandat 456.579 kb on - strandat 456.586 kb on + strandat 456.586 kb on + strandat 456.586 kb on + strandat 456.586 kb on + strandat 456.634 kb on + strand, within Pf1N1B4_387at 456.634 kb on + strand, within Pf1N1B4_387at 456.634 kb on + strand, within Pf1N1B4_387at 456.635 kb on - strand, within Pf1N1B4_387at 456.665 kb on + strand, within Pf1N1B4_387at 456.673 kb on + strand, within Pf1N1B4_387at 456.703 kb on + strandat 456.703 kb on + strandat 456.703 kb on + strandat 456.703 kb on + strandat 456.944 kb on + strandat 456.944 kb on + strand, within Pf1N1B4_388at 456.945 kb on - strand, within Pf1N1B4_388at 456.945 kb on - strand, within Pf1N1B4_388at 456.945 kb on - strand, within Pf1N1B4_388at 456.945 kb on - strand, within Pf1N1B4_388at 456.945 kb on - strandat 456.945 kb on - strand, within Pf1N1B4_388at 456.945 kb on - strand, within Pf1N1B4_388at 456.945 kb on - strand, within Pf1N1B4_388at 457.048 kb on + strand, within Pf1N1B4_388at 457.049 kb on - strand, within Pf1N1B4_388at 457.049 kb on - strand, within Pf1N1B4_388at 457.366 kb on + strand, within Pf1N1B4_388at 457.367 kb on - strand, within Pf1N1B4_388at 457.381 kb on + strand, within Pf1N1B4_388at 457.381 kb on + strand, within Pf1N1B4_388at 457.381 kb on + strand, within Pf1N1B4_388at 457.381 kb on + strand, within Pf1N1B4_388at 457.381 kb on + strand, within Pf1N1B4_388at 457.382 kb on - strand, within Pf1N1B4_388at 457.387 kb on + strand, within Pf1N1B4_388at 457.388 kb on - strand, within Pf1N1B4_388at 457.388 kb on - strand, within Pf1N1B4_388at 457.418 kb on - strand, within Pf1N1B4_388at 457.418 kb on - strand, within Pf1N1B4_388at 457.571 kb on - strandat 457.670 kb on - strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.898 kb on + strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.899 kb on - strand, within Pf1N1B4_389at 457.975 kb on + strandat 457.975 kb on + strandat 457.976 kb on - strandat 457.976 kb on - strandat 457.982 kb on + strandat 457.983 kb on - strandat 457.992 kb on + strandat 458.113 kb on + strand, within Pf1N1B4_390at 458.155 kb on + strand, within Pf1N1B4_390at 458.257 kb on - strand, within Pf1N1B4_390at 458.375 kb on + strand, within Pf1N1B4_390at 458.375 kb on + strand, within Pf1N1B4_390at 458.433 kb on + strand, within Pf1N1B4_390at 458.441 kb on + strand, within Pf1N1B4_390at 458.469 kb on + strand, within Pf1N1B4_390at 458.499 kb on + strand, within Pf1N1B4_390at 458.499 kb on + strandat 458.499 kb on + strand, within Pf1N1B4_390at 458.499 kb on + strand, within Pf1N1B4_390at 458.500 kb on - strand, within Pf1N1B4_390at 458.500 kb on - strand, within Pf1N1B4_390at 458.601 kb on + strand, within Pf1N1B4_390

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM
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455,844 - Pf1N1B4_386 0.47 +3.0
455,844 - Pf1N1B4_386 0.47 -1.5
455,989 + Pf1N1B4_386 0.57 -1.3
455,992 + Pf1N1B4_386 0.57 +1.2
455,992 + Pf1N1B4_386 0.57 -0.7
455,992 + Pf1N1B4_386 0.57 -0.7
455,992 + +1.1
455,992 + Pf1N1B4_386 0.57 +2.1
455,992 + Pf1N1B4_386 0.57 -2.2
455,992 + Pf1N1B4_386 0.57 +0.7
455,992 + Pf1N1B4_386 0.57 +1.6
455,992 + Pf1N1B4_386 0.57 -1.3
455,992 + Pf1N1B4_386 0.57 -1.0
456,036 + Pf1N1B4_386 0.60 +0.4
456,172 + Pf1N1B4_386 0.70 -0.4
456,172 + Pf1N1B4_386 0.70 -2.3
456,172 + Pf1N1B4_386 0.70 +0.4
456,172 + -1.3
456,172 + Pf1N1B4_386 0.70 +0.4
456,172 + Pf1N1B4_386 0.70 -2.9
456,172 + Pf1N1B4_386 0.70 +0.9
456,173 - Pf1N1B4_386 0.70 -0.5
456,173 - Pf1N1B4_386 0.70 -0.5
456,173 - Pf1N1B4_386 0.70 -0.7
456,173 - Pf1N1B4_386 0.70 -0.9
456,173 - Pf1N1B4_386 0.70 -3.2
456,245 - Pf1N1B4_386 0.75 -0.9
456,245 - Pf1N1B4_386 0.75 -1.0
456,245 - Pf1N1B4_386 0.75 -1.8
456,245 - Pf1N1B4_386 0.75 -2.1
456,403 + Pf1N1B4_386 0.86 -0.4
456,403 + Pf1N1B4_386 0.86 -0.9
456,403 + Pf1N1B4_386 0.86 -2.2
456,403 + Pf1N1B4_386 0.86 -2.6
456,403 + Pf1N1B4_386 0.86 -2.0
456,403 + Pf1N1B4_386 0.86 -0.9
456,404 - Pf1N1B4_386 0.86 -0.9
456,404 - Pf1N1B4_386 0.86 +0.5
456,538 + +1.5
456,538 + -0.1
456,539 - -2.4
456,539 - +1.6
456,578 + +0.8
456,578 + -3.4
456,578 + -2.2
456,578 + +0.3
456,579 - -0.9
456,579 - -1.3
456,579 - -1.9
456,579 - +1.9
456,586 + +1.2
456,586 + +2.1
456,586 + +0.7
456,586 + +2.8
456,634 + Pf1N1B4_387 0.46 -1.3
456,634 + Pf1N1B4_387 0.46 +2.0
456,634 + Pf1N1B4_387 0.46 -1.4
456,635 - Pf1N1B4_387 0.46 -1.2
456,665 + Pf1N1B4_387 0.73 -1.5
456,673 + Pf1N1B4_387 0.80 +0.3
456,703 + +1.1
456,703 + -0.2
456,703 + +2.4
456,703 + -0.9
456,944 + -1.0
456,944 + Pf1N1B4_388 0.16 +0.8
456,945 - Pf1N1B4_388 0.16 -1.0
456,945 - Pf1N1B4_388 0.16 -1.4
456,945 - Pf1N1B4_388 0.16 -0.3
456,945 - Pf1N1B4_388 0.16 -1.0
456,945 - -1.0
456,945 - Pf1N1B4_388 0.16 +1.8
456,945 - Pf1N1B4_388 0.16 +1.5
456,945 - Pf1N1B4_388 0.16 +1.7
457,048 + Pf1N1B4_388 0.29 +4.1
457,049 - Pf1N1B4_388 0.29 +0.9
457,049 - Pf1N1B4_388 0.29 +1.2
457,366 + Pf1N1B4_388 0.68 +2.8
457,367 - Pf1N1B4_388 0.68 +1.2
457,381 + Pf1N1B4_388 0.69 +1.6
457,381 + Pf1N1B4_388 0.69 +1.4
457,381 + Pf1N1B4_388 0.69 -0.4
457,381 + Pf1N1B4_388 0.69 -2.1
457,381 + Pf1N1B4_388 0.69 +1.8
457,382 - Pf1N1B4_388 0.70 +2.7
457,387 + Pf1N1B4_388 0.70 +0.5
457,388 - Pf1N1B4_388 0.70 +0.2
457,388 - Pf1N1B4_388 0.70 +0.0
457,418 - Pf1N1B4_388 0.74 -0.4
457,418 - Pf1N1B4_388 0.74 +2.3
457,571 - +1.4
457,670 - Pf1N1B4_389 0.13 -0.6
457,898 + Pf1N1B4_389 0.76 +1.4
457,898 + Pf1N1B4_389 0.76 +1.6
457,898 + Pf1N1B4_389 0.76 +2.4
457,898 + Pf1N1B4_389 0.76 +0.7
457,898 + Pf1N1B4_389 0.76 -0.5
457,898 + Pf1N1B4_389 0.76 +2.7
457,898 + Pf1N1B4_389 0.76 +2.6
457,898 + Pf1N1B4_389 0.76 -3.0
457,898 + Pf1N1B4_389 0.76 -0.9
457,898 + Pf1N1B4_389 0.76 +1.1
457,898 + Pf1N1B4_389 0.76 +2.5
457,898 + Pf1N1B4_389 0.76 +1.3
457,898 + Pf1N1B4_389 0.76 -0.9
457,898 + Pf1N1B4_389 0.76 +2.7
457,898 + Pf1N1B4_389 0.76 +1.0
457,899 - Pf1N1B4_389 0.77 +1.8
457,899 - Pf1N1B4_389 0.77 +1.0
457,899 - Pf1N1B4_389 0.77 +0.8
457,899 - Pf1N1B4_389 0.77 +0.1
457,899 - Pf1N1B4_389 0.77 -0.9
457,899 - Pf1N1B4_389 0.77 +1.4
457,899 - Pf1N1B4_389 0.77 -0.5
457,975 + +1.8
457,975 + +0.3
457,976 - +0.6
457,976 - +1.3
457,982 + +0.7
457,983 - +1.2
457,992 + +0.6
458,113 + Pf1N1B4_390 0.15 -0.9
458,155 + Pf1N1B4_390 0.20 +1.5
458,257 - Pf1N1B4_390 0.32 -0.0
458,375 + Pf1N1B4_390 0.46 +1.5
458,375 + Pf1N1B4_390 0.46 +2.2
458,433 + Pf1N1B4_390 0.53 +1.1
458,441 + Pf1N1B4_390 0.54 +0.0
458,469 + Pf1N1B4_390 0.57 +1.3
458,499 + Pf1N1B4_390 0.61 +1.6
458,499 + +1.3
458,499 + Pf1N1B4_390 0.61 +2.3
458,499 + Pf1N1B4_390 0.61 +1.2
458,500 - Pf1N1B4_390 0.61 -2.6
458,500 - Pf1N1B4_390 0.61 +0.6
458,601 + Pf1N1B4_390 0.73 +0.6

Or see this region's nucleotide sequence