Experiment: carbon source Sucrose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2891 and Pf1N1B4_2892 are separated by 101 nucleotides Pf1N1B4_2892 and Pf1N1B4_2893 are separated by 183 nucleotides Pf1N1B4_2893 and Pf1N1B4_2894 are separated by 7 nucleotides Pf1N1B4_2894 and Pf1N1B4_2895 are separated by 179 nucleotides
Pf1N1B4_2891 - Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-), at 3,245,918 to 3,246,370
_2891
Pf1N1B4_2892 - Phosphotransferase system, HPr-related proteins, at 3,246,472 to 3,246,876
_2892
Pf1N1B4_2893 - Carbonic anhydrase (EC 4.2.1.1), at 3,247,060 to 3,247,704
_2893
Pf1N1B4_2894 - FIG00956317: hypothetical protein, at 3,247,712 to 3,248,236
_2894
Pf1N1B4_2895 - FIG00953119: hypothetical protein, at 3,248,416 to 3,249,591
_2895
Position (kb)
3247
3248 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 3246.200 kb on + strand, within Pf1N1B4_2891 at 3246.201 kb on - strand, within Pf1N1B4_2891 at 3246.266 kb on + strand, within Pf1N1B4_2891 at 3246.266 kb on + strand, within Pf1N1B4_2891 at 3246.266 kb on + strand, within Pf1N1B4_2891 at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.355 kb on + strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.356 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.890 kb on - strand at 3246.894 kb on + strand at 3246.895 kb on - strand at 3246.895 kb on - strand at 3246.895 kb on - strand at 3246.901 kb on + strand at 3246.902 kb on - strand at 3246.904 kb on + strand at 3246.905 kb on - strand at 3246.964 kb on + strand at 3246.964 kb on + strand at 3246.964 kb on + strand at 3246.965 kb on - strand at 3246.965 kb on - strand at 3246.965 kb on - strand at 3246.965 kb on - strand at 3246.986 kb on + strand at 3247.070 kb on + strand at 3247.070 kb on + strand at 3247.070 kb on + strand at 3247.071 kb on - strand at 3247.071 kb on - strand at 3247.086 kb on + strand at 3247.145 kb on + strand, within Pf1N1B4_2893 at 3247.146 kb on - strand, within Pf1N1B4_2893 at 3247.146 kb on - strand, within Pf1N1B4_2893 at 3247.168 kb on + strand, within Pf1N1B4_2893 at 3247.168 kb on + strand, within Pf1N1B4_2893 at 3247.168 kb on + strand, within Pf1N1B4_2893 at 3247.252 kb on - strand, within Pf1N1B4_2893 at 3247.307 kb on - strand, within Pf1N1B4_2893 at 3247.370 kb on + strand, within Pf1N1B4_2893 at 3247.370 kb on + strand, within Pf1N1B4_2893 at 3247.371 kb on - strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.441 kb on + strand, within Pf1N1B4_2893 at 3247.441 kb on + strand, within Pf1N1B4_2893 at 3247.442 kb on - strand, within Pf1N1B4_2893 at 3247.542 kb on + strand, within Pf1N1B4_2893 at 3247.542 kb on + strand, within Pf1N1B4_2893 at 3247.603 kb on + strand, within Pf1N1B4_2893 at 3247.603 kb on + strand, within Pf1N1B4_2893 at 3247.603 kb on + strand at 3247.603 kb on + strand, within Pf1N1B4_2893 at 3247.604 kb on - strand, within Pf1N1B4_2893 at 3247.604 kb on - strand, within Pf1N1B4_2893 at 3247.604 kb on - strand, within Pf1N1B4_2893 at 3247.676 kb on - strand at 3247.676 kb on - strand at 3247.702 kb on + strand at 3247.702 kb on + strand at 3247.702 kb on + strand at 3247.702 kb on + strand at 3247.702 kb on + strand at 3247.703 kb on - strand at 3247.703 kb on - strand at 3247.703 kb on - strand at 3247.703 kb on - strand at 3247.703 kb on - strand at 3247.983 kb on + strand, within Pf1N1B4_2894 at 3247.984 kb on - strand, within Pf1N1B4_2894 at 3247.984 kb on - strand, within Pf1N1B4_2894 at 3248.121 kb on + strand, within Pf1N1B4_2894 at 3248.121 kb on + strand, within Pf1N1B4_2894 at 3248.121 kb on + strand, within Pf1N1B4_2894 at 3248.121 kb on + strand, within Pf1N1B4_2894 at 3248.122 kb on - strand, within Pf1N1B4_2894 at 3248.174 kb on + strand, within Pf1N1B4_2894 at 3248.184 kb on + strand at 3248.184 kb on + strand at 3248.185 kb on - strand at 3248.185 kb on - strand at 3248.236 kb on + strand at 3248.236 kb on + strand at 3248.260 kb on + strand at 3248.260 kb on + strand at 3248.273 kb on + strand at 3248.305 kb on + strand at 3248.305 kb on + strand at 3248.305 kb on + strand at 3248.305 kb on + strand at 3248.305 kb on + strand at 3248.306 kb on - strand at 3248.306 kb on - strand at 3248.340 kb on + strand at 3248.350 kb on + strand at 3248.351 kb on - strand at 3248.417 kb on + strand at 3248.417 kb on + strand at 3248.417 kb on + strand at 3248.417 kb on + strand at 3248.417 kb on + strand at 3248.418 kb on - strand at 3248.546 kb on + strand, within Pf1N1B4_2895 at 3248.546 kb on + strand, within Pf1N1B4_2895 at 3248.546 kb on + strand, within Pf1N1B4_2895 at 3248.546 kb on + strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand at 3248.596 kb on - strand, within Pf1N1B4_2895
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM remove 3,246,200 + Pf1N1B4_2891 0.62 -0.3 3,246,201 - Pf1N1B4_2891 0.62 +2.1 3,246,266 + Pf1N1B4_2891 0.77 -1.7 3,246,266 + Pf1N1B4_2891 0.77 +0.6 3,246,266 + Pf1N1B4_2891 0.77 -0.9 3,246,355 + +0.6 3,246,355 + -1.2 3,246,355 + +0.8 3,246,355 + -0.4 3,246,355 + -1.9 3,246,355 + +0.5 3,246,355 + -0.9 3,246,355 + -0.1 3,246,355 + +1.6 3,246,355 + +1.7 3,246,356 - +1.6 3,246,356 - +2.0 3,246,356 - -2.4 3,246,356 - +1.5 3,246,356 - -2.2 3,246,356 - -0.7 3,246,356 - +0.4 3,246,356 - -1.5 3,246,890 - +2.1 3,246,890 - +1.3 3,246,890 - -1.2 3,246,890 - +2.2 3,246,890 - -1.8 3,246,890 - +1.6 3,246,894 + -1.6 3,246,895 - -0.6 3,246,895 - +0.9 3,246,895 - +0.0 3,246,901 + +2.2 3,246,902 - +0.0 3,246,904 + -0.5 3,246,905 - -1.1 3,246,964 + -0.8 3,246,964 + -0.3 3,246,964 + -1.5 3,246,965 - +1.4 3,246,965 - +0.5 3,246,965 - -2.7 3,246,965 - -2.3 3,246,986 + -2.9 3,247,070 + -1.7 3,247,070 + -1.7 3,247,070 + +0.6 3,247,071 - +0.0 3,247,071 - -1.7 3,247,086 + -0.2 3,247,145 + Pf1N1B4_2893 0.13 -1.1 3,247,146 - Pf1N1B4_2893 0.13 -0.9 3,247,146 - Pf1N1B4_2893 0.13 -2.8 3,247,168 + Pf1N1B4_2893 0.17 -4.3 3,247,168 + Pf1N1B4_2893 0.17 -5.4 3,247,168 + Pf1N1B4_2893 0.17 -0.2 3,247,252 - Pf1N1B4_2893 0.30 -2.1 3,247,307 - Pf1N1B4_2893 0.38 -2.2 3,247,370 + Pf1N1B4_2893 0.48 -1.7 3,247,370 + Pf1N1B4_2893 0.48 -0.4 3,247,371 - Pf1N1B4_2893 0.48 -1.4 3,247,438 + Pf1N1B4_2893 0.59 -1.7 3,247,438 + Pf1N1B4_2893 0.59 -1.8 3,247,438 + Pf1N1B4_2893 0.59 -2.2 3,247,438 + Pf1N1B4_2893 0.59 -1.9 3,247,438 + Pf1N1B4_2893 0.59 -1.4 3,247,438 + Pf1N1B4_2893 0.59 -1.5 3,247,439 - Pf1N1B4_2893 0.59 -3.7 3,247,439 - Pf1N1B4_2893 0.59 -0.9 3,247,439 - Pf1N1B4_2893 0.59 -0.4 3,247,439 - Pf1N1B4_2893 0.59 -1.2 3,247,439 - Pf1N1B4_2893 0.59 -2.2 3,247,439 - Pf1N1B4_2893 0.59 -1.4 3,247,441 + Pf1N1B4_2893 0.59 -1.8 3,247,441 + Pf1N1B4_2893 0.59 -1.5 3,247,442 - Pf1N1B4_2893 0.59 +0.0 3,247,542 + Pf1N1B4_2893 0.75 -2.0 3,247,542 + Pf1N1B4_2893 0.75 -1.0 3,247,603 + Pf1N1B4_2893 0.84 -1.2 3,247,603 + Pf1N1B4_2893 0.84 -3.0 3,247,603 + -1.1 3,247,603 + Pf1N1B4_2893 0.84 -1.9 3,247,604 - Pf1N1B4_2893 0.84 -2.0 3,247,604 - Pf1N1B4_2893 0.84 -2.1 3,247,604 - Pf1N1B4_2893 0.84 -3.5 3,247,676 - -1.8 3,247,676 - -1.4 3,247,702 + +1.0 3,247,702 + -2.7 3,247,702 + -0.8 3,247,702 + -1.9 3,247,702 + +1.2 3,247,703 - -1.2 3,247,703 - -0.5 3,247,703 - +0.5 3,247,703 - -1.9 3,247,703 - -1.7 3,247,983 + Pf1N1B4_2894 0.52 +0.4 3,247,984 - Pf1N1B4_2894 0.52 -1.7 3,247,984 - Pf1N1B4_2894 0.52 -1.2 3,248,121 + Pf1N1B4_2894 0.78 -1.2 3,248,121 + Pf1N1B4_2894 0.78 +2.2 3,248,121 + Pf1N1B4_2894 0.78 +3.7 3,248,121 + Pf1N1B4_2894 0.78 +0.4 3,248,122 - Pf1N1B4_2894 0.78 +1.2 3,248,174 + Pf1N1B4_2894 0.88 -2.1 3,248,184 + -2.1 3,248,184 + -1.3 3,248,185 - -1.9 3,248,185 - +2.0 3,248,236 + -0.6 3,248,236 + +2.7 3,248,260 + +2.1 3,248,260 + -1.6 3,248,273 + -2.4 3,248,305 + -1.7 3,248,305 + +0.8 3,248,305 + -1.9 3,248,305 + -0.4 3,248,305 + -2.4 3,248,306 - -0.6 3,248,306 - -1.1 3,248,340 + -1.9 3,248,350 + +1.3 3,248,351 - +3.0 3,248,417 + +0.9 3,248,417 + -0.0 3,248,417 + -1.4 3,248,417 + +0.8 3,248,417 + -1.2 3,248,418 - -0.9 3,248,546 + Pf1N1B4_2895 0.11 -3.1 3,248,546 + Pf1N1B4_2895 0.11 -1.2 3,248,546 + Pf1N1B4_2895 0.11 +1.7 3,248,546 + Pf1N1B4_2895 0.11 -2.4 3,248,547 - Pf1N1B4_2895 0.11 +0.6 3,248,547 - Pf1N1B4_2895 0.11 -1.8 3,248,547 - Pf1N1B4_2895 0.11 -0.1 3,248,547 - Pf1N1B4_2895 0.11 +1.3 3,248,547 - Pf1N1B4_2895 0.11 +1.2 3,248,547 - -0.8 3,248,596 - Pf1N1B4_2895 0.15 +1.0
Or see this region's nucleotide sequence