Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2750

Experiment: carbon source Sucrose 0.25mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2748 and Pf1N1B4_2749 are separated by 75 nucleotidesPf1N1B4_2749 and Pf1N1B4_2750 are separated by 5 nucleotidesPf1N1B4_2750 and Pf1N1B4_2751 are separated by 178 nucleotides Pf1N1B4_2748 - Transcriptional regulator, TetR family, at 3,088,303 to 3,088,980 _2748 Pf1N1B4_2749 - Quinate/shikimate 5-dehydrogenase I delta (EC 1.1.1.25), at 3,089,056 to 3,089,910 _2749 Pf1N1B4_2750 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 3,089,916 to 3,090,356 _2750 Pf1N1B4_2751 - Transcriptional regulator, LysR family, at 3,090,535 to 3,091,446 _2751 Position (kb) 3089 3090 3091Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 3089.007 kb on - strandat 3089.226 kb on - strand, within Pf1N1B4_2749at 3089.226 kb on - strand, within Pf1N1B4_2749at 3089.226 kb on - strand, within Pf1N1B4_2749at 3089.226 kb on - strand, within Pf1N1B4_2749at 3089.314 kb on - strand, within Pf1N1B4_2749at 3089.455 kb on + strand, within Pf1N1B4_2749at 3089.455 kb on + strand, within Pf1N1B4_2749at 3089.456 kb on - strand, within Pf1N1B4_2749at 3089.456 kb on - strand, within Pf1N1B4_2749at 3089.456 kb on - strand, within Pf1N1B4_2749at 3089.456 kb on - strand, within Pf1N1B4_2749at 3089.471 kb on - strand, within Pf1N1B4_2749at 3089.515 kb on + strand, within Pf1N1B4_2749at 3089.516 kb on - strand, within Pf1N1B4_2749at 3089.516 kb on - strand, within Pf1N1B4_2749at 3089.805 kb on - strand, within Pf1N1B4_2749at 3090.286 kb on + strand, within Pf1N1B4_2750at 3090.286 kb on + strand, within Pf1N1B4_2750at 3090.286 kb on + strand, within Pf1N1B4_2750at 3090.286 kb on + strand, within Pf1N1B4_2750at 3090.286 kb on + strand, within Pf1N1B4_2750at 3090.286 kb on + strand, within Pf1N1B4_2750at 3090.371 kb on + strandat 3090.371 kb on + strandat 3090.372 kb on - strandat 3090.372 kb on - strandat 3090.372 kb on - strandat 3090.372 kb on - strandat 3090.374 kb on + strandat 3090.374 kb on + strandat 3090.374 kb on + strandat 3090.374 kb on + strandat 3090.375 kb on - strandat 3090.519 kb on + strandat 3090.519 kb on + strandat 3090.520 kb on - strandat 3090.520 kb on - strandat 3090.701 kb on + strand, within Pf1N1B4_2751at 3090.701 kb on + strand, within Pf1N1B4_2751at 3090.751 kb on + strand, within Pf1N1B4_2751at 3090.751 kb on + strand, within Pf1N1B4_2751at 3090.751 kb on + strand, within Pf1N1B4_2751at 3090.752 kb on - strand, within Pf1N1B4_2751at 3090.752 kb on - strand, within Pf1N1B4_2751at 3090.752 kb on - strand, within Pf1N1B4_2751at 3090.770 kb on + strand, within Pf1N1B4_2751at 3090.770 kb on + strand, within Pf1N1B4_2751at 3090.770 kb on + strandat 3090.770 kb on + strand, within Pf1N1B4_2751at 3090.770 kb on + strand, within Pf1N1B4_2751at 3090.770 kb on + strand, within Pf1N1B4_2751at 3090.770 kb on + strand, within Pf1N1B4_2751at 3090.770 kb on + strand, within Pf1N1B4_2751at 3090.770 kb on + strand, within Pf1N1B4_2751at 3090.770 kb on + strand, within Pf1N1B4_2751at 3090.770 kb on + strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.771 kb on - strand, within Pf1N1B4_2751at 3090.854 kb on + strand, within Pf1N1B4_2751at 3090.918 kb on - strand, within Pf1N1B4_2751at 3090.918 kb on - strand, within Pf1N1B4_2751at 3091.099 kb on + strand, within Pf1N1B4_2751at 3091.124 kb on + strand, within Pf1N1B4_2751at 3091.124 kb on + strand, within Pf1N1B4_2751at 3091.124 kb on + strand, within Pf1N1B4_2751at 3091.124 kb on + strand, within Pf1N1B4_2751at 3091.124 kb on + strand, within Pf1N1B4_2751at 3091.124 kb on + strand, within Pf1N1B4_2751at 3091.125 kb on - strand, within Pf1N1B4_2751at 3091.125 kb on - strand, within Pf1N1B4_2751at 3091.125 kb on - strand, within Pf1N1B4_2751at 3091.125 kb on - strand, within Pf1N1B4_2751at 3091.125 kb on - strand, within Pf1N1B4_2751at 3091.235 kb on + strand, within Pf1N1B4_2751

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM
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3,089,007 - -1.9
3,089,226 - Pf1N1B4_2749 0.20 +0.8
3,089,226 - Pf1N1B4_2749 0.20 +2.1
3,089,226 - Pf1N1B4_2749 0.20 -1.5
3,089,226 - Pf1N1B4_2749 0.20 +0.6
3,089,314 - Pf1N1B4_2749 0.30 +0.6
3,089,455 + Pf1N1B4_2749 0.47 -0.4
3,089,455 + Pf1N1B4_2749 0.47 -2.1
3,089,456 - Pf1N1B4_2749 0.47 -0.8
3,089,456 - Pf1N1B4_2749 0.47 -0.8
3,089,456 - Pf1N1B4_2749 0.47 -2.4
3,089,456 - Pf1N1B4_2749 0.47 -1.9
3,089,471 - Pf1N1B4_2749 0.49 +0.1
3,089,515 + Pf1N1B4_2749 0.54 -1.7
3,089,516 - Pf1N1B4_2749 0.54 -0.3
3,089,516 - Pf1N1B4_2749 0.54 -0.1
3,089,805 - Pf1N1B4_2749 0.88 +0.5
3,090,286 + Pf1N1B4_2750 0.84 +0.0
3,090,286 + Pf1N1B4_2750 0.84 -0.3
3,090,286 + Pf1N1B4_2750 0.84 -1.3
3,090,286 + Pf1N1B4_2750 0.84 -1.9
3,090,286 + Pf1N1B4_2750 0.84 -1.8
3,090,286 + Pf1N1B4_2750 0.84 -1.0
3,090,371 + -0.8
3,090,371 + +0.6
3,090,372 - -0.8
3,090,372 - +0.5
3,090,372 - +1.7
3,090,372 - -1.9
3,090,374 + +0.4
3,090,374 + -0.5
3,090,374 + -0.5
3,090,374 + -0.7
3,090,375 - -1.4
3,090,519 + +1.5
3,090,519 + -1.3
3,090,520 - +0.9
3,090,520 - -1.9
3,090,701 + Pf1N1B4_2751 0.18 +3.3
3,090,701 + Pf1N1B4_2751 0.18 -0.1
3,090,751 + Pf1N1B4_2751 0.24 -1.8
3,090,751 + Pf1N1B4_2751 0.24 +2.5
3,090,751 + Pf1N1B4_2751 0.24 -0.7
3,090,752 - Pf1N1B4_2751 0.24 -1.2
3,090,752 - Pf1N1B4_2751 0.24 +1.0
3,090,752 - Pf1N1B4_2751 0.24 -0.4
3,090,770 + Pf1N1B4_2751 0.26 -0.2
3,090,770 + Pf1N1B4_2751 0.26 +3.2
3,090,770 + +2.6
3,090,770 + Pf1N1B4_2751 0.26 +0.2
3,090,770 + Pf1N1B4_2751 0.26 -1.5
3,090,770 + Pf1N1B4_2751 0.26 +0.8
3,090,770 + Pf1N1B4_2751 0.26 -1.8
3,090,770 + Pf1N1B4_2751 0.26 +2.6
3,090,770 + Pf1N1B4_2751 0.26 -0.4
3,090,770 + Pf1N1B4_2751 0.26 +2.2
3,090,770 + Pf1N1B4_2751 0.26 +2.3
3,090,771 - Pf1N1B4_2751 0.26 -1.8
3,090,771 - Pf1N1B4_2751 0.26 -0.4
3,090,771 - Pf1N1B4_2751 0.26 +2.7
3,090,771 - Pf1N1B4_2751 0.26 +0.8
3,090,771 - Pf1N1B4_2751 0.26 +3.8
3,090,771 - Pf1N1B4_2751 0.26 -1.4
3,090,771 - Pf1N1B4_2751 0.26 -0.5
3,090,771 - Pf1N1B4_2751 0.26 +4.3
3,090,771 - Pf1N1B4_2751 0.26 +1.6
3,090,771 - Pf1N1B4_2751 0.26 -0.8
3,090,771 - Pf1N1B4_2751 0.26 +1.3
3,090,771 - Pf1N1B4_2751 0.26 -1.8
3,090,771 - Pf1N1B4_2751 0.26 +2.2
3,090,854 + Pf1N1B4_2751 0.35 -0.8
3,090,918 - Pf1N1B4_2751 0.42 -0.2
3,090,918 - Pf1N1B4_2751 0.42 -1.0
3,091,099 + Pf1N1B4_2751 0.62 +1.0
3,091,124 + Pf1N1B4_2751 0.65 +1.9
3,091,124 + Pf1N1B4_2751 0.65 -2.6
3,091,124 + Pf1N1B4_2751 0.65 -0.2
3,091,124 + Pf1N1B4_2751 0.65 +2.3
3,091,124 + Pf1N1B4_2751 0.65 -1.5
3,091,124 + Pf1N1B4_2751 0.65 +1.7
3,091,125 - Pf1N1B4_2751 0.65 +0.4
3,091,125 - Pf1N1B4_2751 0.65 -1.3
3,091,125 - Pf1N1B4_2751 0.65 +0.2
3,091,125 - Pf1N1B4_2751 0.65 +0.5
3,091,125 - Pf1N1B4_2751 0.65 -2.0
3,091,235 + Pf1N1B4_2751 0.77 -1.7

Or see this region's nucleotide sequence