Experiment: carbon source Sucrose 0.25mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1524 and Pf1N1B4_1525 are separated by 381 nucleotides Pf1N1B4_1525 and Pf1N1B4_1526 are separated by 35 nucleotides Pf1N1B4_1526 and Pf1N1B4_1527 are separated by 369 nucleotides
Pf1N1B4_1524 - Permease of the drug/metabolite transporter (DMT) superfamily, at 1,755,913 to 1,756,809
_1524
Pf1N1B4_1525 - hypothetical protein, at 1,757,191 to 1,757,448
_1525
Pf1N1B4_1526 - hypothetical protein, at 1,757,484 to 1,757,612
_1526
Pf1N1B4_1527 - Queuosine Biosynthesis QueC ATPase, at 1,757,982 to 1,758,671
_1527
Position (kb)
1757
1758 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 1756.211 kb on + strand, within Pf1N1B4_1524 at 1756.212 kb on - strand, within Pf1N1B4_1524 at 1756.409 kb on - strand, within Pf1N1B4_1524 at 1756.409 kb on - strand, within Pf1N1B4_1524 at 1756.409 kb on - strand, within Pf1N1B4_1524 at 1756.441 kb on + strand, within Pf1N1B4_1524 at 1756.442 kb on - strand, within Pf1N1B4_1524 at 1756.442 kb on - strand, within Pf1N1B4_1524 at 1756.466 kb on - strand, within Pf1N1B4_1524 at 1756.466 kb on - strand, within Pf1N1B4_1524 at 1756.466 kb on - strand, within Pf1N1B4_1524 at 1756.466 kb on - strand, within Pf1N1B4_1524 at 1756.466 kb on - strand, within Pf1N1B4_1524 at 1756.501 kb on + strand, within Pf1N1B4_1524 at 1756.501 kb on + strand, within Pf1N1B4_1524 at 1756.501 kb on + strand, within Pf1N1B4_1524 at 1756.501 kb on + strand, within Pf1N1B4_1524 at 1756.501 kb on + strand, within Pf1N1B4_1524 at 1756.501 kb on + strand, within Pf1N1B4_1524 at 1756.502 kb on - strand, within Pf1N1B4_1524 at 1756.502 kb on - strand, within Pf1N1B4_1524 at 1756.502 kb on - strand, within Pf1N1B4_1524 at 1756.502 kb on - strand, within Pf1N1B4_1524 at 1756.502 kb on - strand, within Pf1N1B4_1524 at 1756.502 kb on - strand, within Pf1N1B4_1524 at 1756.502 kb on - strand, within Pf1N1B4_1524 at 1756.502 kb on - strand, within Pf1N1B4_1524 at 1756.502 kb on - strand, within Pf1N1B4_1524 at 1756.609 kb on + strand, within Pf1N1B4_1524 at 1756.609 kb on + strand, within Pf1N1B4_1524 at 1756.609 kb on + strand, within Pf1N1B4_1524 at 1756.609 kb on + strand, within Pf1N1B4_1524 at 1756.609 kb on + strand, within Pf1N1B4_1524 at 1756.610 kb on - strand, within Pf1N1B4_1524 at 1756.610 kb on - strand, within Pf1N1B4_1524 at 1756.610 kb on - strand, within Pf1N1B4_1524 at 1756.610 kb on - strand, within Pf1N1B4_1524 at 1756.610 kb on - strand, within Pf1N1B4_1524 at 1756.610 kb on - strand at 1756.610 kb on - strand, within Pf1N1B4_1524 at 1756.610 kb on - strand, within Pf1N1B4_1524 at 1756.610 kb on - strand at 1756.610 kb on - strand, within Pf1N1B4_1524 at 1756.610 kb on - strand, within Pf1N1B4_1524 at 1756.610 kb on - strand, within Pf1N1B4_1524 at 1756.610 kb on - strand, within Pf1N1B4_1524 at 1756.610 kb on - strand, within Pf1N1B4_1524 at 1756.613 kb on + strand at 1756.613 kb on + strand, within Pf1N1B4_1524 at 1756.613 kb on + strand, within Pf1N1B4_1524 at 1756.613 kb on + strand, within Pf1N1B4_1524 at 1756.613 kb on + strand, within Pf1N1B4_1524 at 1756.613 kb on + strand, within Pf1N1B4_1524 at 1756.614 kb on - strand, within Pf1N1B4_1524 at 1756.614 kb on - strand, within Pf1N1B4_1524 at 1756.614 kb on - strand, within Pf1N1B4_1524 at 1756.614 kb on - strand, within Pf1N1B4_1524 at 1756.614 kb on - strand, within Pf1N1B4_1524 at 1756.614 kb on - strand, within Pf1N1B4_1524 at 1756.654 kb on + strand, within Pf1N1B4_1524 at 1756.654 kb on + strand, within Pf1N1B4_1524 at 1756.755 kb on - strand at 1756.811 kb on + strand at 1756.828 kb on + strand at 1756.828 kb on + strand at 1756.828 kb on + strand at 1756.828 kb on + strand at 1756.828 kb on + strand at 1756.828 kb on + strand at 1756.828 kb on + strand at 1756.828 kb on + strand at 1756.829 kb on - strand at 1756.829 kb on - strand at 1756.859 kb on + strand at 1756.859 kb on + strand at 1756.860 kb on - strand at 1756.909 kb on + strand at 1756.910 kb on - strand at 1756.910 kb on - strand at 1756.938 kb on + strand at 1756.939 kb on - strand at 1756.939 kb on - strand at 1756.939 kb on - strand at 1757.049 kb on - strand at 1757.049 kb on - strand at 1757.056 kb on + strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.057 kb on - strand at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.236 kb on + strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.237 kb on - strand, within Pf1N1B4_1525 at 1757.240 kb on + strand, within Pf1N1B4_1525 at 1757.241 kb on - strand, within Pf1N1B4_1525 at 1757.368 kb on + strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.369 kb on - strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.396 kb on + strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.397 kb on - strand, within Pf1N1B4_1525 at 1757.432 kb on + strand at 1757.432 kb on + strand at 1757.432 kb on + strand at 1757.432 kb on + strand at 1757.433 kb on - strand at 1757.610 kb on + strand at 1757.610 kb on + strand at 1757.610 kb on + strand at 1757.636 kb on - strand at 1757.636 kb on - strand at 1757.636 kb on - strand at 1757.636 kb on - strand at 1757.818 kb on + strand at 1757.818 kb on + strand at 1757.820 kb on + strand at 1757.820 kb on + strand at 1757.821 kb on - strand at 1757.921 kb on + strand at 1757.922 kb on - strand at 1757.988 kb on - strand at 1758.312 kb on + strand, within Pf1N1B4_1527 at 1758.313 kb on - strand, within Pf1N1B4_1527 at 1758.440 kb on + strand, within Pf1N1B4_1527
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.25mM remove 1,756,211 + Pf1N1B4_1524 0.33 +2.0 1,756,212 - Pf1N1B4_1524 0.33 +0.8 1,756,409 - Pf1N1B4_1524 0.55 +2.0 1,756,409 - Pf1N1B4_1524 0.55 +2.6 1,756,409 - Pf1N1B4_1524 0.55 +2.4 1,756,441 + Pf1N1B4_1524 0.59 -1.3 1,756,442 - Pf1N1B4_1524 0.59 +3.7 1,756,442 - Pf1N1B4_1524 0.59 +1.4 1,756,466 - Pf1N1B4_1524 0.62 -2.1 1,756,466 - Pf1N1B4_1524 0.62 +1.8 1,756,466 - Pf1N1B4_1524 0.62 +0.7 1,756,466 - Pf1N1B4_1524 0.62 -2.0 1,756,466 - Pf1N1B4_1524 0.62 +3.2 1,756,501 + Pf1N1B4_1524 0.66 -1.0 1,756,501 + Pf1N1B4_1524 0.66 -1.4 1,756,501 + Pf1N1B4_1524 0.66 -2.6 1,756,501 + Pf1N1B4_1524 0.66 +2.3 1,756,501 + Pf1N1B4_1524 0.66 -3.0 1,756,501 + Pf1N1B4_1524 0.66 -1.8 1,756,502 - Pf1N1B4_1524 0.66 +1.7 1,756,502 - Pf1N1B4_1524 0.66 -1.0 1,756,502 - Pf1N1B4_1524 0.66 +0.9 1,756,502 - Pf1N1B4_1524 0.66 +2.5 1,756,502 - Pf1N1B4_1524 0.66 +1.8 1,756,502 - Pf1N1B4_1524 0.66 +1.1 1,756,502 - Pf1N1B4_1524 0.66 +1.3 1,756,502 - Pf1N1B4_1524 0.66 -2.5 1,756,502 - Pf1N1B4_1524 0.66 +1.6 1,756,609 + Pf1N1B4_1524 0.78 -0.5 1,756,609 + Pf1N1B4_1524 0.78 -1.5 1,756,609 + Pf1N1B4_1524 0.78 -1.6 1,756,609 + Pf1N1B4_1524 0.78 -0.5 1,756,609 + Pf1N1B4_1524 0.78 -1.9 1,756,610 - Pf1N1B4_1524 0.78 -1.7 1,756,610 - Pf1N1B4_1524 0.78 -1.1 1,756,610 - Pf1N1B4_1524 0.78 +2.7 1,756,610 - Pf1N1B4_1524 0.78 -0.3 1,756,610 - Pf1N1B4_1524 0.78 -2.0 1,756,610 - +0.3 1,756,610 - Pf1N1B4_1524 0.78 -2.5 1,756,610 - Pf1N1B4_1524 0.78 +0.7 1,756,610 - +0.3 1,756,610 - Pf1N1B4_1524 0.78 -0.5 1,756,610 - Pf1N1B4_1524 0.78 -1.2 1,756,610 - Pf1N1B4_1524 0.78 +1.1 1,756,610 - Pf1N1B4_1524 0.78 +3.6 1,756,610 - Pf1N1B4_1524 0.78 -0.7 1,756,613 + +1.5 1,756,613 + Pf1N1B4_1524 0.78 -0.8 1,756,613 + Pf1N1B4_1524 0.78 -2.1 1,756,613 + Pf1N1B4_1524 0.78 +0.4 1,756,613 + Pf1N1B4_1524 0.78 -0.8 1,756,613 + Pf1N1B4_1524 0.78 +2.5 1,756,614 - Pf1N1B4_1524 0.78 -1.6 1,756,614 - Pf1N1B4_1524 0.78 +0.6 1,756,614 - Pf1N1B4_1524 0.78 +0.9 1,756,614 - Pf1N1B4_1524 0.78 +0.6 1,756,614 - Pf1N1B4_1524 0.78 -2.1 1,756,614 - Pf1N1B4_1524 0.78 -0.7 1,756,654 + Pf1N1B4_1524 0.83 -1.1 1,756,654 + Pf1N1B4_1524 0.83 -0.5 1,756,755 - +0.3 1,756,811 + +0.0 1,756,828 + -0.8 1,756,828 + +0.7 1,756,828 + -0.7 1,756,828 + -0.2 1,756,828 + -1.3 1,756,828 + -0.5 1,756,828 + -1.3 1,756,828 + -2.7 1,756,829 - +3.1 1,756,829 - -1.1 1,756,859 + -1.6 1,756,859 + +0.6 1,756,860 - -0.5 1,756,909 + +2.3 1,756,910 - -0.8 1,756,910 - -1.7 1,756,938 + +2.5 1,756,939 - +1.9 1,756,939 - +0.6 1,756,939 - -1.1 1,757,049 - +2.2 1,757,049 - -0.8 1,757,056 + +1.6 1,757,057 - -1.3 1,757,057 - -0.4 1,757,057 - -1.4 1,757,057 - -0.4 1,757,057 - -0.8 1,757,236 + Pf1N1B4_1525 0.17 +0.2 1,757,236 + Pf1N1B4_1525 0.17 -2.4 1,757,236 + Pf1N1B4_1525 0.17 -1.2 1,757,236 + Pf1N1B4_1525 0.17 -2.9 1,757,236 + Pf1N1B4_1525 0.17 -1.4 1,757,236 + Pf1N1B4_1525 0.17 +0.2 1,757,236 + Pf1N1B4_1525 0.17 +2.9 1,757,236 + Pf1N1B4_1525 0.17 +0.7 1,757,237 - Pf1N1B4_1525 0.18 +1.1 1,757,237 - Pf1N1B4_1525 0.18 +1.1 1,757,237 - Pf1N1B4_1525 0.18 -1.2 1,757,237 - Pf1N1B4_1525 0.18 -2.2 1,757,237 - Pf1N1B4_1525 0.18 -0.9 1,757,240 + Pf1N1B4_1525 0.19 -2.6 1,757,241 - Pf1N1B4_1525 0.19 -1.0 1,757,368 + Pf1N1B4_1525 0.69 -0.7 1,757,369 - Pf1N1B4_1525 0.69 +1.6 1,757,369 - Pf1N1B4_1525 0.69 +2.6 1,757,369 - Pf1N1B4_1525 0.69 -0.5 1,757,369 - Pf1N1B4_1525 0.69 +0.5 1,757,396 + Pf1N1B4_1525 0.79 -1.5 1,757,396 + Pf1N1B4_1525 0.79 -2.7 1,757,396 + Pf1N1B4_1525 0.79 +0.6 1,757,396 + Pf1N1B4_1525 0.79 -1.2 1,757,397 - Pf1N1B4_1525 0.80 +1.7 1,757,397 - Pf1N1B4_1525 0.80 -0.5 1,757,397 - Pf1N1B4_1525 0.80 -1.5 1,757,397 - Pf1N1B4_1525 0.80 -2.1 1,757,397 - Pf1N1B4_1525 0.80 -0.1 1,757,432 + -2.8 1,757,432 + +5.1 1,757,432 + +1.9 1,757,432 + +0.7 1,757,433 - +2.0 1,757,610 + -1.1 1,757,610 + -1.9 1,757,610 + +2.1 1,757,636 - -1.3 1,757,636 - -1.3 1,757,636 - -1.3 1,757,636 - +0.1 1,757,818 + -0.7 1,757,818 + -1.5 1,757,820 + +4.5 1,757,820 + -2.0 1,757,821 - -0.2 1,757,921 + +0.6 1,757,922 - -1.2 1,757,988 - +0.0 1,758,312 + Pf1N1B4_1527 0.48 -1.9 1,758,313 - Pf1N1B4_1527 0.48 +3.9 1,758,440 + Pf1N1B4_1527 0.66 -3.0
Or see this region's nucleotide sequence