Experiment: KB with Dihydrodehydroerosone 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1554 and Psyr_1555 are separated by 937 nucleotides Psyr_1555 and Psyr_1556 are separated by 373 nucleotides
Psyr_1554: Psyr_1554 - hypothetical protein, at 1,746,859 to 1,747,890
_1554
Psyr_1555: Psyr_1555 - Cobyrinic acid a,c-diamide synthase, at 1,748,828 to 1,749,769
_1555
Psyr_1556: Psyr_1556 - hypothetical protein, at 1,750,143 to 1,750,403
_1556
Position (kb)
1748
1749
1750 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.849 kb on + strand at 1747.850 kb on - strand at 1747.850 kb on - strand at 1747.893 kb on - strand at 1747.907 kb on + strand at 1747.907 kb on + strand at 1747.912 kb on + strand at 1747.913 kb on - strand at 1747.913 kb on - strand at 1747.937 kb on + strand at 1747.938 kb on - strand at 1747.993 kb on - strand at 1748.080 kb on - strand at 1748.166 kb on + strand at 1748.166 kb on + strand at 1748.167 kb on - strand at 1748.167 kb on - strand at 1748.167 kb on - strand at 1748.200 kb on + strand at 1748.200 kb on + strand at 1748.367 kb on + strand at 1748.401 kb on - strand at 1748.871 kb on + strand at 1749.064 kb on - strand, within Psyr_1555 at 1749.112 kb on - strand, within Psyr_1555 at 1749.150 kb on + strand, within Psyr_1555 at 1749.234 kb on + strand, within Psyr_1555 at 1749.234 kb on + strand, within Psyr_1555 at 1749.234 kb on + strand, within Psyr_1555 at 1749.234 kb on + strand, within Psyr_1555 at 1749.235 kb on - strand, within Psyr_1555 at 1749.276 kb on - strand, within Psyr_1555 at 1749.433 kb on + strand, within Psyr_1555 at 1749.456 kb on - strand, within Psyr_1555 at 1749.493 kb on + strand, within Psyr_1555 at 1749.494 kb on - strand, within Psyr_1555 at 1749.598 kb on + strand, within Psyr_1555 at 1749.604 kb on - strand at 1749.604 kb on - strand, within Psyr_1555 at 1749.633 kb on - strand, within Psyr_1555 at 1749.690 kb on - strand at 1749.701 kb on + strand at 1749.701 kb on + strand at 1749.769 kb on + strand at 1749.769 kb on + strand at 1749.770 kb on - strand at 1749.770 kb on - strand at 1749.773 kb on + strand at 1749.773 kb on + strand at 1749.780 kb on - strand at 1749.797 kb on + strand at 1749.815 kb on - strand at 1749.879 kb on + strand at 1749.950 kb on + strand at 1749.973 kb on + strand at 1750.296 kb on - strand, within Psyr_1556 at 1750.307 kb on + strand, within Psyr_1556 at 1750.307 kb on + strand, within Psyr_1556 at 1750.311 kb on + strand, within Psyr_1556 at 1750.361 kb on + strand, within Psyr_1556 at 1750.376 kb on + strand, within Psyr_1556 at 1750.419 kb on + strand at 1750.446 kb on + strand at 1750.530 kb on + strand at 1750.530 kb on + strand at 1750.649 kb on + strand at 1750.649 kb on + strand at 1750.684 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dihydrodehydroerosone 10 mM remove 1,747,849 + -1.9 1,747,849 + -2.1 1,747,849 + -0.6 1,747,849 + -1.6 1,747,849 + -2.4 1,747,849 + -0.4 1,747,849 + -0.8 1,747,849 + +0.2 1,747,850 - -0.6 1,747,850 - +0.2 1,747,893 - -0.3 1,747,907 + -2.4 1,747,907 + -1.1 1,747,912 + -1.8 1,747,913 - -2.0 1,747,913 - -0.9 1,747,937 + -1.1 1,747,938 - -1.7 1,747,993 - -0.8 1,748,080 - +1.9 1,748,166 + -0.5 1,748,166 + -0.5 1,748,167 - +0.6 1,748,167 - +0.4 1,748,167 - -1.9 1,748,200 + -0.9 1,748,200 + +0.2 1,748,367 + -0.8 1,748,401 - -0.9 1,748,871 + -0.4 1,749,064 - Psyr_1555 0.25 -0.4 1,749,112 - Psyr_1555 0.30 +1.2 1,749,150 + Psyr_1555 0.34 -3.2 1,749,234 + Psyr_1555 0.43 -3.2 1,749,234 + Psyr_1555 0.43 +0.5 1,749,234 + Psyr_1555 0.43 -1.1 1,749,234 + Psyr_1555 0.43 -1.8 1,749,235 - Psyr_1555 0.43 -0.8 1,749,276 - Psyr_1555 0.48 -1.1 1,749,433 + Psyr_1555 0.64 -1.0 1,749,456 - Psyr_1555 0.67 +0.4 1,749,493 + Psyr_1555 0.71 -3.0 1,749,494 - Psyr_1555 0.71 -0.1 1,749,598 + Psyr_1555 0.82 -0.4 1,749,604 - -1.4 1,749,604 - Psyr_1555 0.82 -2.2 1,749,633 - Psyr_1555 0.85 -2.0 1,749,690 - +0.8 1,749,701 + -1.8 1,749,701 + -0.6 1,749,769 + -1.8 1,749,769 + -0.8 1,749,770 - -3.4 1,749,770 - +1.6 1,749,773 + +0.5 1,749,773 + -1.5 1,749,780 - +0.2 1,749,797 + -2.0 1,749,815 - -0.8 1,749,879 + -0.4 1,749,950 + -2.0 1,749,973 + -2.8 1,750,296 - Psyr_1556 0.59 -0.8 1,750,307 + Psyr_1556 0.63 -2.0 1,750,307 + Psyr_1556 0.63 -0.7 1,750,311 + Psyr_1556 0.64 -1.4 1,750,361 + Psyr_1556 0.84 -2.4 1,750,376 + Psyr_1556 0.89 -0.9 1,750,419 + -0.8 1,750,446 + -0.9 1,750,530 + +0.2 1,750,530 + -2.0 1,750,649 + -2.0 1,750,649 + -1.2 1,750,684 - -0.6
Or see this region's nucleotide sequence