Experiment: KB with Dihydrodehydroerosone 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0091 and Psyr_0092 are separated by 286 nucleotides Psyr_0092 and Psyr_0093 are separated by 45 nucleotides Psyr_0093 and Psyr_RNA1 are separated by 12 nucleotides Psyr_RNA1 and Psyr_0094 are separated by 45 nucleotides
Psyr_0091: Psyr_0091 - succinate semialdehyde dehydrogenase, at 99,948 to 101,390
_0091
Psyr_0092: Psyr_0092 - Lysine exporter protein (LYSE/YGGA), at 101,677 to 102,312
_0092
Psyr_0093: Psyr_0093 - Flavoprotein monooxygenase, at 102,358 to 103,761
_0093
Psyr_RNA1: Psyr_RNA1 - tRNA-Arg, at 103,774 to 103,850
_RNA1
Psyr_0094: Psyr_0094 - hypothetical protein, at 103,896 to 104,600
_0094
Position (kb) 102
103
104 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 101.402 kb on - strand at 101.402 kb on - strand at 101.402 kb on - strand at 101.436 kb on + strand at 101.557 kb on - strand at 101.810 kb on + strand, within Psyr_0092 at 101.810 kb on + strand, within Psyr_0092 at 101.810 kb on + strand, within Psyr_0092 at 101.810 kb on + strand, within Psyr_0092 at 101.810 kb on + strand, within Psyr_0092 at 101.810 kb on + strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.811 kb on - strand, within Psyr_0092 at 101.958 kb on + strand, within Psyr_0092 at 101.959 kb on - strand, within Psyr_0092 at 101.959 kb on - strand, within Psyr_0092 at 101.959 kb on - strand, within Psyr_0092 at 101.959 kb on - strand, within Psyr_0092 at 102.301 kb on + strand at 102.302 kb on - strand at 102.302 kb on - strand at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.554 kb on + strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.555 kb on - strand, within Psyr_0093 at 102.585 kb on + strand, within Psyr_0093 at 102.618 kb on - strand, within Psyr_0093 at 102.618 kb on - strand, within Psyr_0093 at 102.618 kb on - strand, within Psyr_0093 at 102.618 kb on - strand, within Psyr_0093 at 102.618 kb on - strand, within Psyr_0093 at 102.618 kb on - strand, within Psyr_0093 at 102.882 kb on + strand, within Psyr_0093 at 102.883 kb on - strand, within Psyr_0093 at 102.883 kb on - strand, within Psyr_0093 at 102.948 kb on + strand, within Psyr_0093 at 102.948 kb on + strand, within Psyr_0093 at 102.949 kb on - strand, within Psyr_0093 at 102.949 kb on - strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.950 kb on + strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 102.951 kb on - strand, within Psyr_0093 at 103.182 kb on - strand, within Psyr_0093 at 103.182 kb on - strand, within Psyr_0093 at 103.208 kb on + strand, within Psyr_0093 at 103.208 kb on + strand, within Psyr_0093 at 103.208 kb on + strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.209 kb on - strand, within Psyr_0093 at 103.524 kb on + strand, within Psyr_0093 at 103.524 kb on + strand, within Psyr_0093 at 103.524 kb on + strand, within Psyr_0093 at 103.524 kb on + strand, within Psyr_0093 at 103.525 kb on - strand, within Psyr_0093 at 103.525 kb on - strand, within Psyr_0093 at 103.525 kb on - strand, within Psyr_0093 at 103.569 kb on + strand, within Psyr_0093 at 103.569 kb on + strand, within Psyr_0093 at 103.570 kb on - strand, within Psyr_0093 at 103.570 kb on - strand, within Psyr_0093 at 103.605 kb on + strand, within Psyr_0093 at 103.605 kb on + strand, within Psyr_0093 at 103.756 kb on + strand at 103.756 kb on + strand at 103.851 kb on + strand at 103.851 kb on + strand at 103.851 kb on + strand at 103.852 kb on - strand at 103.852 kb on - strand at 103.852 kb on - strand at 103.852 kb on - strand at 103.852 kb on - strand at 103.852 kb on - strand at 103.867 kb on + strand at 103.868 kb on - strand at 103.868 kb on - strand at 103.890 kb on + strand at 103.890 kb on + strand at 103.891 kb on - strand at 103.940 kb on + strand at 103.940 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.955 kb on + strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 103.956 kb on - strand at 104.019 kb on + strand, within Psyr_0094 at 104.019 kb on + strand, within Psyr_0094 at 104.019 kb on + strand, within Psyr_0094 at 104.020 kb on - strand, within Psyr_0094 at 104.020 kb on - strand, within Psyr_0094 at 104.020 kb on - strand, within Psyr_0094 at 104.072 kb on - strand, within Psyr_0094 at 104.072 kb on - strand, within Psyr_0094 at 104.072 kb on - strand, within Psyr_0094 at 104.075 kb on + strand, within Psyr_0094 at 104.075 kb on + strand, within Psyr_0094 at 104.075 kb on + strand, within Psyr_0094 at 104.075 kb on + strand, within Psyr_0094 at 104.076 kb on - strand, within Psyr_0094 at 104.076 kb on - strand, within Psyr_0094 at 104.076 kb on - strand, within Psyr_0094 at 104.130 kb on + strand, within Psyr_0094 at 104.130 kb on + strand, within Psyr_0094 at 104.131 kb on - strand, within Psyr_0094 at 104.280 kb on + strand, within Psyr_0094 at 104.281 kb on - strand, within Psyr_0094 at 104.281 kb on - strand, within Psyr_0094 at 104.281 kb on - strand, within Psyr_0094 at 104.378 kb on + strand, within Psyr_0094 at 104.378 kb on + strand, within Psyr_0094 at 104.378 kb on + strand, within Psyr_0094 at 104.378 kb on + strand, within Psyr_0094 at 104.379 kb on - strand, within Psyr_0094 at 104.379 kb on - strand, within Psyr_0094 at 104.379 kb on - strand, within Psyr_0094 at 104.401 kb on + strand, within Psyr_0094 at 104.431 kb on + strand, within Psyr_0094 at 104.432 kb on - strand, within Psyr_0094 at 104.439 kb on + strand, within Psyr_0094 at 104.439 kb on + strand, within Psyr_0094 at 104.440 kb on - strand, within Psyr_0094 at 104.473 kb on + strand, within Psyr_0094 at 104.473 kb on + strand, within Psyr_0094 at 104.473 kb on + strand, within Psyr_0094 at 104.474 kb on - strand, within Psyr_0094 at 104.474 kb on - strand, within Psyr_0094 at 104.474 kb on - strand, within Psyr_0094 at 104.474 kb on - strand, within Psyr_0094 at 104.474 kb on - strand, within Psyr_0094 at 104.562 kb on + strand at 104.573 kb on + strand at 104.573 kb on + strand at 104.573 kb on + strand at 104.574 kb on - strand at 104.574 kb on - strand at 104.574 kb on - strand at 104.574 kb on - strand at 104.574 kb on - strand at 104.665 kb on - strand at 104.684 kb on + strand at 104.684 kb on + strand at 104.685 kb on - strand at 104.716 kb on + strand at 104.716 kb on + strand at 104.716 kb on + strand at 104.716 kb on + strand at 104.716 kb on + strand at 104.716 kb on + strand at 104.716 kb on + strand at 104.717 kb on - strand at 104.717 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Dihydrodehydroerosone 10 mM remove 101,402 - -0.7 101,402 - -0.7 101,402 - -0.4 101,436 + -0.0 101,557 - -0.2 101,810 + Psyr_0092 0.21 +0.3 101,810 + Psyr_0092 0.21 -0.5 101,810 + Psyr_0092 0.21 +0.5 101,810 + Psyr_0092 0.21 +0.2 101,810 + Psyr_0092 0.21 -1.1 101,810 + Psyr_0092 0.21 +0.1 101,811 - Psyr_0092 0.21 +0.1 101,811 - Psyr_0092 0.21 +0.2 101,811 - Psyr_0092 0.21 -0.8 101,811 - Psyr_0092 0.21 +0.2 101,811 - Psyr_0092 0.21 -1.7 101,811 - Psyr_0092 0.21 -0.1 101,958 + Psyr_0092 0.44 -0.4 101,959 - Psyr_0092 0.44 +0.5 101,959 - Psyr_0092 0.44 -0.7 101,959 - Psyr_0092 0.44 +0.3 101,959 - Psyr_0092 0.44 -0.1 102,301 + +2.2 102,302 - +0.5 102,302 - +1.2 102,554 + Psyr_0093 0.14 -0.1 102,554 + Psyr_0093 0.14 +0.7 102,554 + Psyr_0093 0.14 +0.2 102,554 + Psyr_0093 0.14 +0.3 102,554 + Psyr_0093 0.14 +0.1 102,554 + Psyr_0093 0.14 -0.1 102,554 + Psyr_0093 0.14 +0.1 102,554 + Psyr_0093 0.14 -0.0 102,554 + Psyr_0093 0.14 +0.6 102,554 + Psyr_0093 0.14 -0.5 102,554 + Psyr_0093 0.14 +1.3 102,554 + Psyr_0093 0.14 -0.2 102,554 + Psyr_0093 0.14 +0.6 102,555 - Psyr_0093 0.14 +1.7 102,555 - Psyr_0093 0.14 -0.7 102,555 - Psyr_0093 0.14 +0.4 102,555 - Psyr_0093 0.14 +1.0 102,555 - Psyr_0093 0.14 -1.2 102,555 - Psyr_0093 0.14 +0.1 102,555 - Psyr_0093 0.14 -0.7 102,555 - Psyr_0093 0.14 +0.4 102,555 - Psyr_0093 0.14 +0.6 102,555 - Psyr_0093 0.14 +0.7 102,555 - Psyr_0093 0.14 -0.3 102,585 + Psyr_0093 0.16 +0.2 102,618 - Psyr_0093 0.19 +1.1 102,618 - Psyr_0093 0.19 +0.8 102,618 - Psyr_0093 0.19 +0.7 102,618 - Psyr_0093 0.19 +1.4 102,618 - Psyr_0093 0.19 +0.6 102,618 - Psyr_0093 0.19 +0.5 102,882 + Psyr_0093 0.37 -0.5 102,883 - Psyr_0093 0.37 -0.5 102,883 - Psyr_0093 0.37 +1.2 102,948 + Psyr_0093 0.42 -0.0 102,948 + Psyr_0093 0.42 -0.4 102,949 - Psyr_0093 0.42 -0.2 102,949 - Psyr_0093 0.42 -0.0 102,950 + Psyr_0093 0.42 +0.2 102,950 + Psyr_0093 0.42 -0.8 102,950 + Psyr_0093 0.42 -1.4 102,950 + Psyr_0093 0.42 +0.1 102,950 + Psyr_0093 0.42 -0.6 102,950 + Psyr_0093 0.42 -0.6 102,950 + Psyr_0093 0.42 +1.3 102,951 - Psyr_0093 0.42 -1.2 102,951 - Psyr_0093 0.42 +1.4 102,951 - Psyr_0093 0.42 +1.4 102,951 - Psyr_0093 0.42 -0.4 102,951 - Psyr_0093 0.42 -2.0 102,951 - Psyr_0093 0.42 -1.0 102,951 - Psyr_0093 0.42 +1.0 103,182 - Psyr_0093 0.59 +0.6 103,182 - Psyr_0093 0.59 -0.0 103,208 + Psyr_0093 0.61 +1.0 103,208 + Psyr_0093 0.61 -0.2 103,208 + Psyr_0093 0.61 +0.8 103,209 - Psyr_0093 0.61 -0.2 103,209 - Psyr_0093 0.61 +0.6 103,209 - Psyr_0093 0.61 +0.5 103,209 - Psyr_0093 0.61 +0.3 103,209 - Psyr_0093 0.61 -0.7 103,209 - Psyr_0093 0.61 -0.2 103,209 - Psyr_0093 0.61 +0.0 103,209 - Psyr_0093 0.61 +0.2 103,524 + Psyr_0093 0.83 -0.2 103,524 + Psyr_0093 0.83 +0.4 103,524 + Psyr_0093 0.83 +0.1 103,524 + Psyr_0093 0.83 +0.2 103,525 - Psyr_0093 0.83 +0.3 103,525 - Psyr_0093 0.83 -0.6 103,525 - Psyr_0093 0.83 +0.1 103,569 + Psyr_0093 0.86 -0.2 103,569 + Psyr_0093 0.86 -0.2 103,570 - Psyr_0093 0.86 -0.4 103,570 - Psyr_0093 0.86 +0.4 103,605 + Psyr_0093 0.89 +0.2 103,605 + Psyr_0093 0.89 -0.4 103,756 + -0.8 103,756 + -0.2 103,851 + +1.0 103,851 + +0.4 103,851 + +0.6 103,852 - +0.3 103,852 - -0.0 103,852 - -0.6 103,852 - +0.1 103,852 - -1.9 103,852 - -0.5 103,867 + +0.4 103,868 - +0.4 103,868 - +3.7 103,890 + +0.2 103,890 + -0.9 103,891 - +0.3 103,940 + -0.5 103,940 + -0.5 103,955 + -0.3 103,955 + -0.1 103,955 + +0.0 103,955 + -0.1 103,955 + +0.3 103,955 + +3.7 103,955 + -0.5 103,955 + -0.5 103,955 + +0.6 103,955 + -0.4 103,955 + -0.0 103,955 + -0.3 103,955 + -0.2 103,955 + -0.0 103,956 - +0.4 103,956 - -0.6 103,956 - +0.1 103,956 - +0.3 103,956 - -0.2 103,956 - -1.2 103,956 - -0.6 103,956 - -0.7 103,956 - -0.6 103,956 - -1.2 103,956 - +1.1 103,956 - +0.8 103,956 - -0.4 103,956 - +0.2 103,956 - -0.9 104,019 + Psyr_0094 0.17 +0.2 104,019 + Psyr_0094 0.17 +0.3 104,019 + Psyr_0094 0.17 +0.9 104,020 - Psyr_0094 0.18 +1.4 104,020 - Psyr_0094 0.18 -0.3 104,020 - Psyr_0094 0.18 -0.6 104,072 - Psyr_0094 0.25 +2.3 104,072 - Psyr_0094 0.25 -0.6 104,072 - Psyr_0094 0.25 -0.7 104,075 + Psyr_0094 0.25 -0.2 104,075 + Psyr_0094 0.25 +0.7 104,075 + Psyr_0094 0.25 -1.0 104,075 + Psyr_0094 0.25 +0.4 104,076 - Psyr_0094 0.26 -0.9 104,076 - Psyr_0094 0.26 +0.5 104,076 - Psyr_0094 0.26 +0.7 104,130 + Psyr_0094 0.33 -1.2 104,130 + Psyr_0094 0.33 +0.5 104,131 - Psyr_0094 0.33 -0.2 104,280 + Psyr_0094 0.54 +1.3 104,281 - Psyr_0094 0.55 -0.5 104,281 - Psyr_0094 0.55 +1.4 104,281 - Psyr_0094 0.55 +0.8 104,378 + Psyr_0094 0.68 -0.3 104,378 + Psyr_0094 0.68 +0.3 104,378 + Psyr_0094 0.68 +0.1 104,378 + Psyr_0094 0.68 +0.4 104,379 - Psyr_0094 0.69 -0.1 104,379 - Psyr_0094 0.69 +1.4 104,379 - Psyr_0094 0.69 -0.7 104,401 + Psyr_0094 0.72 -0.2 104,431 + Psyr_0094 0.76 +0.4 104,432 - Psyr_0094 0.76 +0.5 104,439 + Psyr_0094 0.77 -0.6 104,439 + Psyr_0094 0.77 +2.7 104,440 - Psyr_0094 0.77 +0.6 104,473 + Psyr_0094 0.82 -0.2 104,473 + Psyr_0094 0.82 +2.7 104,473 + Psyr_0094 0.82 +0.0 104,474 - Psyr_0094 0.82 -0.3 104,474 - Psyr_0094 0.82 +1.6 104,474 - Psyr_0094 0.82 -0.6 104,474 - Psyr_0094 0.82 -1.3 104,474 - Psyr_0094 0.82 +1.4 104,562 + +0.8 104,573 + -0.4 104,573 + -0.1 104,573 + +0.2 104,574 - +0.3 104,574 - +1.6 104,574 - -1.0 104,574 - +0.3 104,574 - +0.2 104,665 - +0.0 104,684 + +0.2 104,684 + -0.2 104,685 - -0.1 104,716 + -1.6 104,716 + -0.7 104,716 + -0.4 104,716 + -0.1 104,716 + -0.6 104,716 + -1.2 104,716 + +0.7 104,717 - +1.4 104,717 - +0.8
Or see this region's nucleotide sequence