Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3708

Experiment: carbon source Sucrose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3707 and Pf1N1B4_3708 overlap by 4 nucleotidesPf1N1B4_3708 and Pf1N1B4_3709 overlap by 4 nucleotidesPf1N1B4_3709 and Pf1N1B4_3710 overlap by 8 nucleotides Pf1N1B4_3707 - Cobyrinic acid A,C-diamide synthase, at 4,115,664 to 4,117,046 _3707 Pf1N1B4_3708 - Cobalamin biosynthesis protein BluB @ 5,6-dimethylbenzimidazole synthase, flavin destructase family, at 4,117,043 to 4,117,693 _3708 Pf1N1B4_3709 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 4,117,690 to 4,118,598 _3709 Pf1N1B4_3710 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 4,118,591 to 4,119,583 _3710 Position (kb) 4117 4118Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 4116.417 kb on - strand, within Pf1N1B4_3707at 4116.420 kb on + strand, within Pf1N1B4_3707at 4116.517 kb on - strandat 4116.517 kb on - strand, within Pf1N1B4_3707at 4116.550 kb on + strandat 4116.550 kb on + strand, within Pf1N1B4_3707at 4116.550 kb on + strand, within Pf1N1B4_3707at 4116.550 kb on + strand, within Pf1N1B4_3707at 4116.550 kb on + strand, within Pf1N1B4_3707at 4116.550 kb on + strand, within Pf1N1B4_3707at 4116.551 kb on - strand, within Pf1N1B4_3707at 4116.551 kb on - strand, within Pf1N1B4_3707at 4116.642 kb on + strand, within Pf1N1B4_3707at 4116.642 kb on + strand, within Pf1N1B4_3707at 4116.642 kb on + strand, within Pf1N1B4_3707at 4116.643 kb on - strand, within Pf1N1B4_3707at 4116.789 kb on + strand, within Pf1N1B4_3707at 4116.789 kb on + strand, within Pf1N1B4_3707at 4116.789 kb on + strandat 4116.789 kb on + strand, within Pf1N1B4_3707at 4116.789 kb on + strand, within Pf1N1B4_3707at 4116.789 kb on + strand, within Pf1N1B4_3707at 4116.789 kb on + strand, within Pf1N1B4_3707at 4116.900 kb on + strand, within Pf1N1B4_3707at 4116.900 kb on + strand, within Pf1N1B4_3707at 4116.900 kb on + strandat 4116.901 kb on - strand, within Pf1N1B4_3707at 4116.901 kb on - strand, within Pf1N1B4_3707at 4116.912 kb on + strandat 4116.912 kb on + strandat 4117.149 kb on + strand, within Pf1N1B4_3708at 4117.149 kb on + strand, within Pf1N1B4_3708at 4117.359 kb on + strand, within Pf1N1B4_3708at 4117.360 kb on - strand, within Pf1N1B4_3708at 4117.620 kb on + strand, within Pf1N1B4_3708at 4117.664 kb on + strandat 4117.664 kb on + strandat 4117.665 kb on - strandat 4117.673 kb on + strandat 4117.674 kb on - strandat 4117.909 kb on + strand, within Pf1N1B4_3709at 4117.909 kb on + strand, within Pf1N1B4_3709at 4117.942 kb on + strandat 4117.942 kb on + strandat 4117.942 kb on + strand, within Pf1N1B4_3709at 4118.284 kb on + strandat 4118.284 kb on + strand, within Pf1N1B4_3709at 4118.284 kb on + strand, within Pf1N1B4_3709at 4118.284 kb on + strand, within Pf1N1B4_3709at 4118.285 kb on - strand, within Pf1N1B4_3709at 4118.285 kb on - strand, within Pf1N1B4_3709at 4118.315 kb on - strand, within Pf1N1B4_3709at 4118.452 kb on + strand, within Pf1N1B4_3709at 4118.452 kb on + strand, within Pf1N1B4_3709at 4118.452 kb on + strand, within Pf1N1B4_3709at 4118.591 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source Sucrose 0.5mM
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4,116,417 - Pf1N1B4_3707 0.54 -1.4
4,116,420 + Pf1N1B4_3707 0.55 -2.2
4,116,517 - -0.9
4,116,517 - Pf1N1B4_3707 0.62 +0.5
4,116,550 + -0.1
4,116,550 + Pf1N1B4_3707 0.64 -2.2
4,116,550 + Pf1N1B4_3707 0.64 +1.8
4,116,550 + Pf1N1B4_3707 0.64 -1.2
4,116,550 + Pf1N1B4_3707 0.64 -2.8
4,116,550 + Pf1N1B4_3707 0.64 +0.1
4,116,551 - Pf1N1B4_3707 0.64 +1.1
4,116,551 - Pf1N1B4_3707 0.64 -0.9
4,116,642 + Pf1N1B4_3707 0.71 +0.7
4,116,642 + Pf1N1B4_3707 0.71 -1.1
4,116,642 + Pf1N1B4_3707 0.71 +0.8
4,116,643 - Pf1N1B4_3707 0.71 -1.9
4,116,789 + Pf1N1B4_3707 0.81 -0.3
4,116,789 + Pf1N1B4_3707 0.81 -0.1
4,116,789 + -1.8
4,116,789 + Pf1N1B4_3707 0.81 -1.9
4,116,789 + Pf1N1B4_3707 0.81 -2.1
4,116,789 + Pf1N1B4_3707 0.81 +0.3
4,116,789 + Pf1N1B4_3707 0.81 -1.5
4,116,900 + Pf1N1B4_3707 0.89 -2.3
4,116,900 + Pf1N1B4_3707 0.89 +0.5
4,116,900 + +1.9
4,116,901 - Pf1N1B4_3707 0.89 -0.7
4,116,901 - Pf1N1B4_3707 0.89 +3.0
4,116,912 + +0.1
4,116,912 + +1.5
4,117,149 + Pf1N1B4_3708 0.16 +0.9
4,117,149 + Pf1N1B4_3708 0.16 +3.3
4,117,359 + Pf1N1B4_3708 0.49 -0.2
4,117,360 - Pf1N1B4_3708 0.49 -3.2
4,117,620 + Pf1N1B4_3708 0.89 +1.2
4,117,664 + +1.2
4,117,664 + +4.0
4,117,665 - -0.6
4,117,673 + -1.5
4,117,674 - -2.2
4,117,909 + Pf1N1B4_3709 0.24 +1.5
4,117,909 + Pf1N1B4_3709 0.24 -2.0
4,117,942 + +1.8
4,117,942 + +0.2
4,117,942 + Pf1N1B4_3709 0.28 +1.9
4,118,284 + -1.8
4,118,284 + Pf1N1B4_3709 0.65 -2.5
4,118,284 + Pf1N1B4_3709 0.65 -0.4
4,118,284 + Pf1N1B4_3709 0.65 +2.0
4,118,285 - Pf1N1B4_3709 0.65 -1.7
4,118,285 - Pf1N1B4_3709 0.65 +1.7
4,118,315 - Pf1N1B4_3709 0.69 +1.2
4,118,452 + Pf1N1B4_3709 0.84 +0.6
4,118,452 + Pf1N1B4_3709 0.84 +2.9
4,118,452 + Pf1N1B4_3709 0.84 -2.1
4,118,591 - -1.0

Or see this region's nucleotide sequence