Experiment: carbon source Sucrose 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3186 and Pf1N1B4_3187 are separated by 114 nucleotides Pf1N1B4_3187 and Pf1N1B4_3188 are separated by 4 nucleotides Pf1N1B4_3188 and Pf1N1B4_3189 are separated by 202 nucleotides
Pf1N1B4_3186 - Conserved secreted protein, at 3,569,331 to 3,569,816
_3186
Pf1N1B4_3187 - UPF0225 protein YchJ, at 3,569,931 to 3,570,407
_3187
Pf1N1B4_3188 - FIG00953202: hypothetical protein, at 3,570,412 to 3,570,909
_3188
Pf1N1B4_3189 - Outer membrane protein A precursor, at 3,571,112 to 3,571,807
_3189
Position (kb)
3570
3571 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3569.513 kb on + strand, within Pf1N1B4_3186 at 3569.513 kb on + strand, within Pf1N1B4_3186 at 3569.514 kb on - strand, within Pf1N1B4_3186 at 3569.519 kb on - strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.577 kb on + strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.578 kb on - strand, within Pf1N1B4_3186 at 3569.676 kb on + strand, within Pf1N1B4_3186 at 3569.676 kb on + strand, within Pf1N1B4_3186 at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.793 kb on + strand at 3569.794 kb on - strand at 3569.824 kb on + strand at 3569.825 kb on - strand at 3569.845 kb on + strand at 3569.845 kb on + strand at 3569.845 kb on + strand at 3569.879 kb on + strand at 3569.879 kb on + strand at 3569.880 kb on - strand at 3569.944 kb on + strand at 3570.000 kb on - strand, within Pf1N1B4_3187 at 3570.000 kb on - strand, within Pf1N1B4_3187 at 3570.000 kb on - strand, within Pf1N1B4_3187 at 3570.037 kb on + strand, within Pf1N1B4_3187 at 3570.037 kb on + strand, within Pf1N1B4_3187 at 3570.038 kb on - strand, within Pf1N1B4_3187 at 3570.038 kb on - strand, within Pf1N1B4_3187 at 3570.058 kb on - strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.261 kb on + strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.262 kb on - strand, within Pf1N1B4_3187 at 3570.265 kb on + strand, within Pf1N1B4_3187 at 3570.266 kb on - strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.282 kb on + strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.283 kb on - strand, within Pf1N1B4_3187 at 3570.286 kb on + strand, within Pf1N1B4_3187 at 3570.286 kb on + strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.287 kb on - strand, within Pf1N1B4_3187 at 3570.346 kb on + strand, within Pf1N1B4_3187 at 3570.401 kb on + strand at 3570.401 kb on + strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.402 kb on - strand at 3570.422 kb on + strand at 3570.422 kb on + strand at 3570.423 kb on - strand at 3570.423 kb on - strand at 3570.483 kb on + strand, within Pf1N1B4_3188 at 3570.483 kb on + strand, within Pf1N1B4_3188 at 3570.484 kb on - strand, within Pf1N1B4_3188 at 3570.484 kb on - strand, within Pf1N1B4_3188 at 3570.484 kb on - strand, within Pf1N1B4_3188 at 3570.485 kb on + strand at 3570.485 kb on + strand, within Pf1N1B4_3188 at 3570.485 kb on + strand, within Pf1N1B4_3188 at 3570.485 kb on + strand, within Pf1N1B4_3188 at 3570.486 kb on - strand, within Pf1N1B4_3188 at 3570.486 kb on - strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.500 kb on + strand, within Pf1N1B4_3188 at 3570.501 kb on - strand, within Pf1N1B4_3188 at 3570.504 kb on + strand, within Pf1N1B4_3188 at 3570.504 kb on + strand, within Pf1N1B4_3188 at 3570.505 kb on - strand, within Pf1N1B4_3188 at 3570.517 kb on + strand, within Pf1N1B4_3188 at 3570.517 kb on + strand, within Pf1N1B4_3188 at 3570.517 kb on + strand, within Pf1N1B4_3188 at 3570.518 kb on - strand, within Pf1N1B4_3188 at 3570.518 kb on - strand, within Pf1N1B4_3188 at 3570.518 kb on - strand, within Pf1N1B4_3188 at 3570.518 kb on - strand, within Pf1N1B4_3188 at 3570.898 kb on - strand at 3570.898 kb on - strand at 3570.898 kb on - strand at 3570.898 kb on - strand at 3570.938 kb on + strand at 3570.961 kb on + strand at 3570.961 kb on + strand at 3570.961 kb on + strand at 3570.961 kb on + strand at 3570.961 kb on + strand at 3570.962 kb on - strand at 3571.085 kb on - strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.109 kb on + strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.110 kb on - strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.119 kb on + strand at 3571.135 kb on - strand at 3571.135 kb on - strand at 3571.381 kb on + strand, within Pf1N1B4_3189 at 3571.381 kb on + strand, within Pf1N1B4_3189 at 3571.381 kb on + strand, within Pf1N1B4_3189 at 3571.382 kb on - strand, within Pf1N1B4_3189 at 3571.382 kb on - strand at 3571.382 kb on - strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand, within Pf1N1B4_3189 at 3571.533 kb on + strand at 3571.533 kb on - strand, within Pf1N1B4_3189 at 3571.534 kb on - strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.625 kb on + strand, within Pf1N1B4_3189 at 3571.626 kb on - strand at 3571.626 kb on - strand, within Pf1N1B4_3189 at 3571.670 kb on + strand, within Pf1N1B4_3189 at 3571.670 kb on + strand, within Pf1N1B4_3189 at 3571.670 kb on + strand, within Pf1N1B4_3189 at 3571.671 kb on - strand, within Pf1N1B4_3189 at 3571.671 kb on - strand at 3571.671 kb on - strand, within Pf1N1B4_3189 at 3571.699 kb on + strand, within Pf1N1B4_3189 at 3571.699 kb on + strand, within Pf1N1B4_3189 at 3571.699 kb on + strand, within Pf1N1B4_3189 at 3571.700 kb on - strand, within Pf1N1B4_3189 at 3571.700 kb on - strand, within Pf1N1B4_3189 at 3571.700 kb on - strand, within Pf1N1B4_3189 at 3571.700 kb on - strand, within Pf1N1B4_3189 at 3571.805 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.830 kb on + strand at 3571.831 kb on - strand at 3571.831 kb on - strand at 3571.831 kb on - strand at 3571.831 kb on - strand at 3571.852 kb on + strand at 3571.852 kb on + strand at 3571.852 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand at 3571.909 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.5mM remove 3,569,513 + Pf1N1B4_3186 0.37 +1.1 3,569,513 + Pf1N1B4_3186 0.37 -1.3 3,569,514 - Pf1N1B4_3186 0.38 +1.2 3,569,519 - Pf1N1B4_3186 0.39 -1.5 3,569,577 + Pf1N1B4_3186 0.51 -0.9 3,569,577 + Pf1N1B4_3186 0.51 +1.3 3,569,577 + Pf1N1B4_3186 0.51 -1.8 3,569,577 + Pf1N1B4_3186 0.51 -3.7 3,569,577 + Pf1N1B4_3186 0.51 +0.8 3,569,577 + Pf1N1B4_3186 0.51 -0.6 3,569,577 + Pf1N1B4_3186 0.51 -1.5 3,569,577 + Pf1N1B4_3186 0.51 -1.3 3,569,577 + Pf1N1B4_3186 0.51 +0.3 3,569,577 + Pf1N1B4_3186 0.51 -2.6 3,569,577 + Pf1N1B4_3186 0.51 +0.8 3,569,577 + Pf1N1B4_3186 0.51 +1.4 3,569,577 + Pf1N1B4_3186 0.51 -1.3 3,569,577 + Pf1N1B4_3186 0.51 -2.6 3,569,577 + Pf1N1B4_3186 0.51 +1.3 3,569,578 - Pf1N1B4_3186 0.51 +1.2 3,569,578 - Pf1N1B4_3186 0.51 -3.9 3,569,578 - Pf1N1B4_3186 0.51 +0.9 3,569,578 - Pf1N1B4_3186 0.51 +0.3 3,569,578 - Pf1N1B4_3186 0.51 -1.8 3,569,578 - Pf1N1B4_3186 0.51 -2.0 3,569,578 - Pf1N1B4_3186 0.51 +0.9 3,569,578 - Pf1N1B4_3186 0.51 +0.1 3,569,676 + Pf1N1B4_3186 0.71 -2.4 3,569,676 + Pf1N1B4_3186 0.71 +2.5 3,569,793 + +0.4 3,569,793 + -1.5 3,569,793 + +0.6 3,569,793 + -1.7 3,569,794 - -1.2 3,569,824 + -2.1 3,569,825 - +1.7 3,569,845 + -0.3 3,569,845 + +1.0 3,569,845 + +0.9 3,569,879 + +0.5 3,569,879 + +1.1 3,569,880 - +0.5 3,569,944 + -0.3 3,570,000 - Pf1N1B4_3187 0.14 -0.7 3,570,000 - Pf1N1B4_3187 0.14 +0.1 3,570,000 - Pf1N1B4_3187 0.14 +2.0 3,570,037 + Pf1N1B4_3187 0.22 -0.7 3,570,037 + Pf1N1B4_3187 0.22 +0.7 3,570,038 - Pf1N1B4_3187 0.22 -1.2 3,570,038 - Pf1N1B4_3187 0.22 +0.6 3,570,058 - Pf1N1B4_3187 0.27 +2.0 3,570,261 + Pf1N1B4_3187 0.69 -0.2 3,570,261 + Pf1N1B4_3187 0.69 -0.4 3,570,261 + Pf1N1B4_3187 0.69 -2.5 3,570,261 + Pf1N1B4_3187 0.69 +0.1 3,570,262 - Pf1N1B4_3187 0.69 -0.0 3,570,262 - Pf1N1B4_3187 0.69 +0.3 3,570,262 - Pf1N1B4_3187 0.69 -0.3 3,570,262 - Pf1N1B4_3187 0.69 +1.5 3,570,265 + Pf1N1B4_3187 0.70 +0.2 3,570,266 - Pf1N1B4_3187 0.70 +2.1 3,570,282 + Pf1N1B4_3187 0.74 +2.9 3,570,282 + Pf1N1B4_3187 0.74 +0.4 3,570,282 + Pf1N1B4_3187 0.74 -1.6 3,570,282 + Pf1N1B4_3187 0.74 -3.2 3,570,282 + Pf1N1B4_3187 0.74 -2.8 3,570,282 + Pf1N1B4_3187 0.74 +2.5 3,570,282 + Pf1N1B4_3187 0.74 -1.6 3,570,283 - Pf1N1B4_3187 0.74 -1.5 3,570,283 - -1.6 3,570,283 - Pf1N1B4_3187 0.74 +0.7 3,570,283 - Pf1N1B4_3187 0.74 -0.9 3,570,283 - Pf1N1B4_3187 0.74 -1.6 3,570,283 - Pf1N1B4_3187 0.74 -0.2 3,570,283 - Pf1N1B4_3187 0.74 -1.4 3,570,283 - Pf1N1B4_3187 0.74 -0.4 3,570,283 - Pf1N1B4_3187 0.74 -0.5 3,570,286 + Pf1N1B4_3187 0.74 +0.6 3,570,286 + Pf1N1B4_3187 0.74 +1.3 3,570,287 - Pf1N1B4_3187 0.75 -0.4 3,570,287 - Pf1N1B4_3187 0.75 -2.0 3,570,287 - Pf1N1B4_3187 0.75 -0.2 3,570,287 - Pf1N1B4_3187 0.75 -0.4 3,570,346 + Pf1N1B4_3187 0.87 -0.5 3,570,401 + +0.5 3,570,401 + +0.4 3,570,402 - -2.0 3,570,402 - +0.1 3,570,402 - -0.2 3,570,402 - -2.0 3,570,402 - +0.7 3,570,402 - +1.3 3,570,402 - +1.7 3,570,402 - -1.9 3,570,402 - -2.3 3,570,422 + -2.9 3,570,422 + -1.7 3,570,423 - -2.9 3,570,423 - +1.2 3,570,483 + Pf1N1B4_3188 0.14 -3.5 3,570,483 + Pf1N1B4_3188 0.14 +0.5 3,570,484 - Pf1N1B4_3188 0.14 +0.7 3,570,484 - Pf1N1B4_3188 0.14 +0.4 3,570,484 - Pf1N1B4_3188 0.14 -2.5 3,570,485 + -1.4 3,570,485 + Pf1N1B4_3188 0.15 -0.7 3,570,485 + Pf1N1B4_3188 0.15 -1.8 3,570,485 + Pf1N1B4_3188 0.15 -0.1 3,570,486 - Pf1N1B4_3188 0.15 +0.8 3,570,486 - Pf1N1B4_3188 0.15 +1.1 3,570,500 + Pf1N1B4_3188 0.18 +1.2 3,570,500 + Pf1N1B4_3188 0.18 -1.4 3,570,500 + Pf1N1B4_3188 0.18 -1.7 3,570,500 + Pf1N1B4_3188 0.18 -1.0 3,570,500 + Pf1N1B4_3188 0.18 +1.6 3,570,501 - Pf1N1B4_3188 0.18 -0.9 3,570,504 + Pf1N1B4_3188 0.18 +1.3 3,570,504 + Pf1N1B4_3188 0.18 -0.3 3,570,505 - Pf1N1B4_3188 0.19 -0.7 3,570,517 + Pf1N1B4_3188 0.21 -1.7 3,570,517 + Pf1N1B4_3188 0.21 +0.5 3,570,517 + Pf1N1B4_3188 0.21 -1.1 3,570,518 - Pf1N1B4_3188 0.21 +1.7 3,570,518 - Pf1N1B4_3188 0.21 -1.9 3,570,518 - Pf1N1B4_3188 0.21 +2.1 3,570,518 - Pf1N1B4_3188 0.21 -0.4 3,570,898 - -1.0 3,570,898 - +2.4 3,570,898 - -0.5 3,570,898 - +0.2 3,570,938 + -0.7 3,570,961 + -3.0 3,570,961 + -2.4 3,570,961 + -0.5 3,570,961 + -2.0 3,570,961 + +0.1 3,570,962 - +0.2 3,571,085 - -1.8 3,571,109 + +0.4 3,571,109 + +1.6 3,571,109 + +0.1 3,571,109 + -1.4 3,571,109 + +1.1 3,571,109 + -1.4 3,571,110 - +0.2 3,571,110 - +1.7 3,571,110 - +3.3 3,571,110 - +1.4 3,571,110 - +3.6 3,571,110 - +1.6 3,571,110 - -1.8 3,571,110 - +1.7 3,571,119 + -1.0 3,571,119 + -1.9 3,571,119 + -0.6 3,571,119 + -0.1 3,571,119 + +1.4 3,571,119 + -2.0 3,571,119 + -0.7 3,571,119 + -0.6 3,571,135 - -0.4 3,571,135 - -2.9 3,571,381 + Pf1N1B4_3189 0.39 -0.9 3,571,381 + Pf1N1B4_3189 0.39 +1.0 3,571,381 + Pf1N1B4_3189 0.39 -1.0 3,571,382 - Pf1N1B4_3189 0.39 -2.0 3,571,382 - -1.7 3,571,382 - Pf1N1B4_3189 0.39 -0.6 3,571,533 + Pf1N1B4_3189 0.60 +0.9 3,571,533 + Pf1N1B4_3189 0.60 +1.1 3,571,533 + Pf1N1B4_3189 0.60 +0.4 3,571,533 + Pf1N1B4_3189 0.60 +1.1 3,571,533 + Pf1N1B4_3189 0.60 -0.3 3,571,533 + -0.1 3,571,533 - Pf1N1B4_3189 0.60 -1.1 3,571,534 - Pf1N1B4_3189 0.61 -1.4 3,571,625 + Pf1N1B4_3189 0.74 -2.1 3,571,625 + Pf1N1B4_3189 0.74 -2.3 3,571,625 + Pf1N1B4_3189 0.74 -1.5 3,571,625 + Pf1N1B4_3189 0.74 -0.6 3,571,625 + Pf1N1B4_3189 0.74 +1.2 3,571,625 + Pf1N1B4_3189 0.74 +0.9 3,571,626 - -0.6 3,571,626 - Pf1N1B4_3189 0.74 -1.1 3,571,670 + Pf1N1B4_3189 0.80 -1.7 3,571,670 + Pf1N1B4_3189 0.80 -2.5 3,571,670 + Pf1N1B4_3189 0.80 -2.8 3,571,671 - Pf1N1B4_3189 0.80 -1.4 3,571,671 - -1.1 3,571,671 - Pf1N1B4_3189 0.80 +1.3 3,571,699 + Pf1N1B4_3189 0.84 +1.4 3,571,699 + Pf1N1B4_3189 0.84 -2.5 3,571,699 + Pf1N1B4_3189 0.84 +1.3 3,571,700 - Pf1N1B4_3189 0.84 +1.9 3,571,700 - Pf1N1B4_3189 0.84 -1.8 3,571,700 - Pf1N1B4_3189 0.84 -0.7 3,571,700 - Pf1N1B4_3189 0.84 -2.0 3,571,805 + +0.2 3,571,830 + +1.3 3,571,830 + +2.1 3,571,830 + -1.4 3,571,830 + -2.1 3,571,830 + -0.6 3,571,830 + -0.7 3,571,830 + -0.2 3,571,831 - -1.7 3,571,831 - +1.6 3,571,831 - +0.7 3,571,831 - +2.5 3,571,852 + +1.4 3,571,852 + -0.4 3,571,852 + +1.7 3,571,909 + +1.6 3,571,909 + +0.4 3,571,909 + -2.3 3,571,909 + +0.2 3,571,909 + +1.7 3,571,909 + +1.8 3,571,909 + -0.2
Or see this region's nucleotide sequence