Experiment: carbon source Sucrose 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2893 and Pf1N1B4_2894 are separated by 7 nucleotides Pf1N1B4_2894 and Pf1N1B4_2895 are separated by 179 nucleotides Pf1N1B4_2895 and Pf1N1B4_2896 are separated by 242 nucleotides
Pf1N1B4_2893 - Carbonic anhydrase (EC 4.2.1.1), at 3,247,060 to 3,247,704
_2893
Pf1N1B4_2894 - FIG00956317: hypothetical protein, at 3,247,712 to 3,248,236
_2894
Pf1N1B4_2895 - FIG00953119: hypothetical protein, at 3,248,416 to 3,249,591
_2895
Pf1N1B4_2896 - L-Proline/Glycine betaine transporter ProP, at 3,249,834 to 3,251,186
_2896
Position (kb)
3248
3249
3250 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.438 kb on + strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.439 kb on - strand, within Pf1N1B4_2893 at 3247.441 kb on + strand, within Pf1N1B4_2893 at 3247.441 kb on + strand, within Pf1N1B4_2893 at 3247.442 kb on - strand, within Pf1N1B4_2893 at 3247.542 kb on + strand, within Pf1N1B4_2893 at 3247.542 kb on + strand, within Pf1N1B4_2893 at 3247.603 kb on + strand, within Pf1N1B4_2893 at 3247.603 kb on + strand, within Pf1N1B4_2893 at 3247.603 kb on + strand at 3247.603 kb on + strand, within Pf1N1B4_2893 at 3247.604 kb on - strand, within Pf1N1B4_2893 at 3247.604 kb on - strand, within Pf1N1B4_2893 at 3247.604 kb on - strand, within Pf1N1B4_2893 at 3247.676 kb on - strand at 3247.676 kb on - strand at 3247.702 kb on + strand at 3247.702 kb on + strand at 3247.702 kb on + strand at 3247.702 kb on + strand at 3247.702 kb on + strand at 3247.703 kb on - strand at 3247.703 kb on - strand at 3247.703 kb on - strand at 3247.703 kb on - strand at 3247.703 kb on - strand at 3247.983 kb on + strand, within Pf1N1B4_2894 at 3247.984 kb on - strand, within Pf1N1B4_2894 at 3247.984 kb on - strand, within Pf1N1B4_2894 at 3248.121 kb on + strand, within Pf1N1B4_2894 at 3248.121 kb on + strand, within Pf1N1B4_2894 at 3248.121 kb on + strand, within Pf1N1B4_2894 at 3248.121 kb on + strand, within Pf1N1B4_2894 at 3248.122 kb on - strand, within Pf1N1B4_2894 at 3248.174 kb on + strand, within Pf1N1B4_2894 at 3248.184 kb on + strand at 3248.184 kb on + strand at 3248.185 kb on - strand at 3248.185 kb on - strand at 3248.236 kb on + strand at 3248.236 kb on + strand at 3248.260 kb on + strand at 3248.260 kb on + strand at 3248.273 kb on + strand at 3248.305 kb on + strand at 3248.305 kb on + strand at 3248.305 kb on + strand at 3248.305 kb on + strand at 3248.305 kb on + strand at 3248.306 kb on - strand at 3248.306 kb on - strand at 3248.340 kb on + strand at 3248.350 kb on + strand at 3248.351 kb on - strand at 3248.417 kb on + strand at 3248.417 kb on + strand at 3248.417 kb on + strand at 3248.417 kb on + strand at 3248.417 kb on + strand at 3248.418 kb on - strand at 3248.546 kb on + strand, within Pf1N1B4_2895 at 3248.546 kb on + strand, within Pf1N1B4_2895 at 3248.546 kb on + strand, within Pf1N1B4_2895 at 3248.546 kb on + strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand, within Pf1N1B4_2895 at 3248.547 kb on - strand at 3248.596 kb on - strand, within Pf1N1B4_2895 at 3248.732 kb on + strand, within Pf1N1B4_2895 at 3248.733 kb on - strand, within Pf1N1B4_2895 at 3248.733 kb on - strand at 3248.733 kb on - strand, within Pf1N1B4_2895 at 3248.733 kb on - strand, within Pf1N1B4_2895 at 3248.733 kb on - strand, within Pf1N1B4_2895 at 3248.733 kb on - strand, within Pf1N1B4_2895 at 3248.753 kb on + strand, within Pf1N1B4_2895 at 3248.762 kb on + strand, within Pf1N1B4_2895 at 3248.762 kb on + strand, within Pf1N1B4_2895 at 3248.762 kb on + strand, within Pf1N1B4_2895 at 3248.762 kb on + strand, within Pf1N1B4_2895 at 3248.762 kb on + strand, within Pf1N1B4_2895 at 3248.762 kb on + strand, within Pf1N1B4_2895 at 3248.763 kb on - strand at 3248.763 kb on - strand, within Pf1N1B4_2895 at 3248.763 kb on - strand, within Pf1N1B4_2895 at 3248.763 kb on - strand, within Pf1N1B4_2895 at 3248.792 kb on - strand, within Pf1N1B4_2895 at 3248.852 kb on + strand, within Pf1N1B4_2895 at 3248.871 kb on + strand, within Pf1N1B4_2895 at 3248.871 kb on + strand, within Pf1N1B4_2895 at 3248.871 kb on + strand, within Pf1N1B4_2895 at 3248.872 kb on - strand, within Pf1N1B4_2895 at 3248.872 kb on - strand, within Pf1N1B4_2895 at 3248.872 kb on - strand, within Pf1N1B4_2895 at 3248.872 kb on - strand, within Pf1N1B4_2895 at 3248.872 kb on - strand, within Pf1N1B4_2895 at 3248.875 kb on + strand, within Pf1N1B4_2895 at 3248.955 kb on - strand, within Pf1N1B4_2895 at 3248.955 kb on - strand, within Pf1N1B4_2895 at 3248.955 kb on - strand, within Pf1N1B4_2895 at 3248.966 kb on + strand, within Pf1N1B4_2895 at 3248.966 kb on + strand, within Pf1N1B4_2895 at 3248.966 kb on + strand at 3248.967 kb on - strand, within Pf1N1B4_2895 at 3248.967 kb on - strand, within Pf1N1B4_2895 at 3248.972 kb on + strand, within Pf1N1B4_2895 at 3248.972 kb on + strand, within Pf1N1B4_2895 at 3248.972 kb on + strand, within Pf1N1B4_2895 at 3248.973 kb on - strand, within Pf1N1B4_2895 at 3248.973 kb on - strand at 3249.119 kb on + strand, within Pf1N1B4_2895 at 3249.143 kb on + strand, within Pf1N1B4_2895 at 3249.143 kb on + strand, within Pf1N1B4_2895 at 3249.215 kb on + strand, within Pf1N1B4_2895 at 3249.223 kb on - strand, within Pf1N1B4_2895 at 3249.223 kb on - strand at 3249.223 kb on - strand, within Pf1N1B4_2895 at 3249.224 kb on + strand, within Pf1N1B4_2895 at 3249.224 kb on + strand, within Pf1N1B4_2895 at 3249.224 kb on + strand, within Pf1N1B4_2895 at 3249.224 kb on + strand, within Pf1N1B4_2895 at 3249.225 kb on - strand, within Pf1N1B4_2895 at 3249.225 kb on - strand, within Pf1N1B4_2895 at 3249.267 kb on - strand, within Pf1N1B4_2895 at 3249.312 kb on - strand, within Pf1N1B4_2895 at 3249.334 kb on - strand, within Pf1N1B4_2895 at 3249.341 kb on + strand, within Pf1N1B4_2895 at 3249.341 kb on + strand, within Pf1N1B4_2895 at 3249.342 kb on - strand, within Pf1N1B4_2895 at 3249.401 kb on - strand, within Pf1N1B4_2895 at 3249.401 kb on - strand, within Pf1N1B4_2895 at 3249.416 kb on + strand, within Pf1N1B4_2895 at 3249.416 kb on + strand, within Pf1N1B4_2895 at 3249.416 kb on + strand, within Pf1N1B4_2895 at 3249.417 kb on - strand, within Pf1N1B4_2895 at 3249.480 kb on - strand at 3249.546 kb on + strand at 3249.586 kb on + strand at 3249.587 kb on - strand at 3249.587 kb on - strand at 3249.634 kb on + strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.635 kb on - strand at 3249.638 kb on + strand at 3249.638 kb on + strand at 3249.638 kb on + strand at 3249.638 kb on + strand at 3249.639 kb on - strand at 3249.639 kb on - strand at 3249.647 kb on + strand at 3249.647 kb on + strand at 3249.647 kb on + strand at 3249.647 kb on + strand at 3249.647 kb on + strand at 3249.648 kb on - strand at 3249.648 kb on - strand at 3249.828 kb on + strand at 3249.828 kb on + strand at 3249.829 kb on - strand at 3249.921 kb on + strand at 3249.921 kb on + strand at 3249.921 kb on + strand at 3249.921 kb on + strand at 3250.139 kb on + strand, within Pf1N1B4_2896 at 3250.209 kb on + strand, within Pf1N1B4_2896 at 3250.209 kb on + strand, within Pf1N1B4_2896 at 3250.225 kb on - strand, within Pf1N1B4_2896 at 3250.447 kb on - strand, within Pf1N1B4_2896 at 3250.504 kb on + strand, within Pf1N1B4_2896 at 3250.563 kb on - strand, within Pf1N1B4_2896
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.5mM remove 3,247,438 + Pf1N1B4_2893 0.59 -1.7 3,247,438 + Pf1N1B4_2893 0.59 -1.9 3,247,438 + Pf1N1B4_2893 0.59 -2.3 3,247,438 + Pf1N1B4_2893 0.59 -2.0 3,247,438 + Pf1N1B4_2893 0.59 -3.0 3,247,438 + Pf1N1B4_2893 0.59 -4.3 3,247,439 - Pf1N1B4_2893 0.59 -3.7 3,247,439 - Pf1N1B4_2893 0.59 -2.0 3,247,439 - Pf1N1B4_2893 0.59 -0.5 3,247,439 - Pf1N1B4_2893 0.59 -1.3 3,247,439 - Pf1N1B4_2893 0.59 -2.3 3,247,439 - Pf1N1B4_2893 0.59 -1.5 3,247,441 + Pf1N1B4_2893 0.59 -0.3 3,247,441 + Pf1N1B4_2893 0.59 -0.6 3,247,442 - Pf1N1B4_2893 0.59 -2.1 3,247,542 + Pf1N1B4_2893 0.75 -2.1 3,247,542 + Pf1N1B4_2893 0.75 -1.1 3,247,603 + Pf1N1B4_2893 0.84 -2.8 3,247,603 + Pf1N1B4_2893 0.84 -4.1 3,247,603 + -1.2 3,247,603 + Pf1N1B4_2893 0.84 -0.4 3,247,604 - Pf1N1B4_2893 0.84 -2.1 3,247,604 - Pf1N1B4_2893 0.84 -2.1 3,247,604 - Pf1N1B4_2893 0.84 -3.6 3,247,676 - -1.9 3,247,676 - -1.5 3,247,702 + -0.1 3,247,702 + -2.6 3,247,702 + -2.3 3,247,702 + -2.0 3,247,702 + +0.8 3,247,703 - -2.3 3,247,703 - -2.9 3,247,703 - +0.8 3,247,703 - -0.0 3,247,703 - -1.7 3,247,983 + Pf1N1B4_2894 0.52 +0.7 3,247,984 - Pf1N1B4_2894 0.52 -0.2 3,247,984 - Pf1N1B4_2894 0.52 -1.3 3,248,121 + Pf1N1B4_2894 0.78 -3.2 3,248,121 + Pf1N1B4_2894 0.78 -0.8 3,248,121 + Pf1N1B4_2894 0.78 -1.3 3,248,121 + Pf1N1B4_2894 0.78 +1.4 3,248,122 - Pf1N1B4_2894 0.78 +0.3 3,248,174 + Pf1N1B4_2894 0.88 -2.2 3,248,184 + -2.1 3,248,184 + -1.4 3,248,185 - +1.1 3,248,185 - -0.9 3,248,236 + -0.7 3,248,236 + +3.9 3,248,260 + +0.1 3,248,260 + -0.1 3,248,273 + -2.5 3,248,305 + +2.9 3,248,305 + +0.9 3,248,305 + -2.0 3,248,305 + +2.5 3,248,305 + -1.5 3,248,306 - -0.7 3,248,306 - +2.0 3,248,340 + -2.0 3,248,350 + -3.0 3,248,351 - +1.3 3,248,417 + -2.1 3,248,417 + +2.9 3,248,417 + -1.5 3,248,417 + +1.5 3,248,417 + +0.7 3,248,418 - -0.5 3,248,546 + Pf1N1B4_2895 0.11 +0.9 3,248,546 + Pf1N1B4_2895 0.11 -1.3 3,248,546 + Pf1N1B4_2895 0.11 +1.0 3,248,546 + Pf1N1B4_2895 0.11 -2.5 3,248,547 - Pf1N1B4_2895 0.11 +2.8 3,248,547 - Pf1N1B4_2895 0.11 -1.8 3,248,547 - Pf1N1B4_2895 0.11 +0.3 3,248,547 - Pf1N1B4_2895 0.11 -0.6 3,248,547 - Pf1N1B4_2895 0.11 +1.3 3,248,547 - -0.8 3,248,596 - Pf1N1B4_2895 0.15 -1.1 3,248,732 + Pf1N1B4_2895 0.27 -2.5 3,248,733 - Pf1N1B4_2895 0.27 +1.3 3,248,733 - -0.1 3,248,733 - Pf1N1B4_2895 0.27 -2.3 3,248,733 - Pf1N1B4_2895 0.27 +2.0 3,248,733 - Pf1N1B4_2895 0.27 +0.2 3,248,733 - Pf1N1B4_2895 0.27 -1.3 3,248,753 + Pf1N1B4_2895 0.29 -0.5 3,248,762 + Pf1N1B4_2895 0.29 +2.1 3,248,762 + Pf1N1B4_2895 0.29 -1.3 3,248,762 + Pf1N1B4_2895 0.29 -1.4 3,248,762 + Pf1N1B4_2895 0.29 +1.6 3,248,762 + Pf1N1B4_2895 0.29 -0.4 3,248,762 + Pf1N1B4_2895 0.29 -0.8 3,248,763 - -0.7 3,248,763 - Pf1N1B4_2895 0.30 -0.1 3,248,763 - Pf1N1B4_2895 0.30 -0.8 3,248,763 - Pf1N1B4_2895 0.30 +1.6 3,248,792 - Pf1N1B4_2895 0.32 -2.7 3,248,852 + Pf1N1B4_2895 0.37 -1.5 3,248,871 + Pf1N1B4_2895 0.39 -1.1 3,248,871 + Pf1N1B4_2895 0.39 -0.7 3,248,871 + Pf1N1B4_2895 0.39 -2.2 3,248,872 - Pf1N1B4_2895 0.39 +1.1 3,248,872 - Pf1N1B4_2895 0.39 +1.3 3,248,872 - Pf1N1B4_2895 0.39 +2.2 3,248,872 - Pf1N1B4_2895 0.39 -0.5 3,248,872 - Pf1N1B4_2895 0.39 -0.1 3,248,875 + Pf1N1B4_2895 0.39 +0.7 3,248,955 - Pf1N1B4_2895 0.46 -0.6 3,248,955 - Pf1N1B4_2895 0.46 +0.3 3,248,955 - Pf1N1B4_2895 0.46 +1.0 3,248,966 + Pf1N1B4_2895 0.47 -3.2 3,248,966 + Pf1N1B4_2895 0.47 -1.6 3,248,966 + +4.0 3,248,967 - Pf1N1B4_2895 0.47 -2.1 3,248,967 - Pf1N1B4_2895 0.47 +2.3 3,248,972 + Pf1N1B4_2895 0.47 -1.9 3,248,972 + Pf1N1B4_2895 0.47 -1.2 3,248,972 + Pf1N1B4_2895 0.47 +0.6 3,248,973 - Pf1N1B4_2895 0.47 -2.0 3,248,973 - +1.5 3,249,119 + Pf1N1B4_2895 0.60 +0.2 3,249,143 + Pf1N1B4_2895 0.62 +0.4 3,249,143 + Pf1N1B4_2895 0.62 -0.7 3,249,215 + Pf1N1B4_2895 0.68 +1.1 3,249,223 - Pf1N1B4_2895 0.69 +0.2 3,249,223 - -1.2 3,249,223 - Pf1N1B4_2895 0.69 -1.2 3,249,224 + Pf1N1B4_2895 0.69 -0.1 3,249,224 + Pf1N1B4_2895 0.69 +0.8 3,249,224 + Pf1N1B4_2895 0.69 -0.1 3,249,224 + Pf1N1B4_2895 0.69 +0.8 3,249,225 - Pf1N1B4_2895 0.69 -3.0 3,249,225 - Pf1N1B4_2895 0.69 +0.2 3,249,267 - Pf1N1B4_2895 0.72 +0.9 3,249,312 - Pf1N1B4_2895 0.76 +0.8 3,249,334 - Pf1N1B4_2895 0.78 +0.5 3,249,341 + Pf1N1B4_2895 0.79 +1.1 3,249,341 + Pf1N1B4_2895 0.79 +1.0 3,249,342 - Pf1N1B4_2895 0.79 -0.1 3,249,401 - Pf1N1B4_2895 0.84 -1.1 3,249,401 - Pf1N1B4_2895 0.84 -2.0 3,249,416 + Pf1N1B4_2895 0.85 +1.3 3,249,416 + Pf1N1B4_2895 0.85 -0.7 3,249,416 + Pf1N1B4_2895 0.85 +2.0 3,249,417 - Pf1N1B4_2895 0.85 +1.0 3,249,480 - -1.7 3,249,546 + -1.7 3,249,586 + +0.5 3,249,587 - +2.1 3,249,587 - -0.5 3,249,634 + +2.0 3,249,635 - +1.9 3,249,635 - +2.4 3,249,635 - +1.0 3,249,635 - +0.9 3,249,635 - -1.2 3,249,635 - +0.4 3,249,635 - -1.1 3,249,635 - -1.7 3,249,638 + +0.8 3,249,638 + +3.3 3,249,638 + -0.5 3,249,638 + -0.4 3,249,639 - -0.0 3,249,639 - +1.0 3,249,647 + +0.6 3,249,647 + -0.2 3,249,647 + +1.0 3,249,647 + -3.3 3,249,647 + -0.1 3,249,648 - -0.4 3,249,648 - -1.5 3,249,828 + -2.9 3,249,828 + -2.7 3,249,829 - -0.7 3,249,921 + +1.4 3,249,921 + +1.5 3,249,921 + -0.2 3,249,921 + +2.4 3,250,139 + Pf1N1B4_2896 0.23 +0.6 3,250,209 + Pf1N1B4_2896 0.28 -1.1 3,250,209 + Pf1N1B4_2896 0.28 -0.3 3,250,225 - Pf1N1B4_2896 0.29 -1.4 3,250,447 - Pf1N1B4_2896 0.45 +0.7 3,250,504 + Pf1N1B4_2896 0.50 +1.4 3,250,563 - Pf1N1B4_2896 0.54 -1.6
Or see this region's nucleotide sequence