Experiment: carbon source Sucrose 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2720 and Pf1N1B4_2721 are separated by 185 nucleotides Pf1N1B4_2721 and Pf1N1B4_2722 are separated by 71 nucleotides Pf1N1B4_2722 and Pf1N1B4_2723 overlap by 6 nucleotides
Pf1N1B4_2720 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 3,058,059 to 3,059,708
_2720
Pf1N1B4_2721 - AMP nucleosidase (EC 3.2.2.4), at 3,059,894 to 3,061,357
_2721
Pf1N1B4_2722 - Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70), at 3,061,429 to 3,062,064
_2722
Pf1N1B4_2723 - FIG00954142: hypothetical protein, at 3,062,059 to 3,062,268
_2723
Position (kb)
3059
3060
3061
3062 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 3059.220 kb on + strand, within Pf1N1B4_2720 at 3059.220 kb on + strand, within Pf1N1B4_2720 at 3059.220 kb on + strand, within Pf1N1B4_2720 at 3059.221 kb on - strand, within Pf1N1B4_2720 at 3059.221 kb on - strand, within Pf1N1B4_2720 at 3059.287 kb on - strand, within Pf1N1B4_2720 at 3059.377 kb on + strand, within Pf1N1B4_2720 at 3059.377 kb on + strand, within Pf1N1B4_2720 at 3059.377 kb on + strand, within Pf1N1B4_2720 at 3059.377 kb on + strand, within Pf1N1B4_2720 at 3059.378 kb on - strand, within Pf1N1B4_2720 at 3059.378 kb on - strand, within Pf1N1B4_2720 at 3059.378 kb on - strand, within Pf1N1B4_2720 at 3059.566 kb on + strand at 3059.566 kb on + strand at 3059.566 kb on + strand at 3059.567 kb on - strand at 3059.640 kb on + strand at 3059.640 kb on + strand at 3059.640 kb on + strand at 3059.641 kb on - strand at 3059.733 kb on + strand at 3059.734 kb on - strand at 3059.734 kb on - strand at 3059.734 kb on - strand at 3059.734 kb on - strand at 3059.795 kb on + strand at 3059.796 kb on - strand at 3059.796 kb on - strand at 3059.908 kb on + strand at 3059.999 kb on + strand at 3060.000 kb on - strand at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.110 kb on + strand, within Pf1N1B4_2721 at 3060.111 kb on - strand, within Pf1N1B4_2721 at 3060.137 kb on + strand, within Pf1N1B4_2721 at 3060.137 kb on + strand, within Pf1N1B4_2721 at 3060.137 kb on + strand, within Pf1N1B4_2721 at 3060.137 kb on + strand, within Pf1N1B4_2721 at 3060.138 kb on - strand, within Pf1N1B4_2721 at 3060.138 kb on - strand, within Pf1N1B4_2721 at 3060.138 kb on - strand, within Pf1N1B4_2721 at 3060.138 kb on - strand, within Pf1N1B4_2721 at 3060.176 kb on + strand at 3060.176 kb on + strand, within Pf1N1B4_2721 at 3060.176 kb on + strand, within Pf1N1B4_2721 at 3060.176 kb on + strand, within Pf1N1B4_2721 at 3060.177 kb on - strand, within Pf1N1B4_2721 at 3060.177 kb on - strand, within Pf1N1B4_2721 at 3060.263 kb on + strand, within Pf1N1B4_2721 at 3060.337 kb on + strand, within Pf1N1B4_2721 at 3060.380 kb on + strand, within Pf1N1B4_2721 at 3060.381 kb on - strand, within Pf1N1B4_2721 at 3060.464 kb on + strand, within Pf1N1B4_2721 at 3060.465 kb on - strand, within Pf1N1B4_2721 at 3060.465 kb on - strand, within Pf1N1B4_2721 at 3060.465 kb on - strand, within Pf1N1B4_2721 at 3060.515 kb on + strand, within Pf1N1B4_2721 at 3060.515 kb on + strand, within Pf1N1B4_2721 at 3060.515 kb on + strand, within Pf1N1B4_2721 at 3060.516 kb on - strand, within Pf1N1B4_2721 at 3060.689 kb on + strand at 3060.854 kb on + strand, within Pf1N1B4_2721 at 3060.854 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.873 kb on - strand, within Pf1N1B4_2721 at 3060.873 kb on - strand, within Pf1N1B4_2721 at 3060.873 kb on - strand, within Pf1N1B4_2721 at 3060.873 kb on - strand, within Pf1N1B4_2721 at 3061.034 kb on + strand, within Pf1N1B4_2721 at 3061.034 kb on + strand, within Pf1N1B4_2721 at 3061.034 kb on + strand, within Pf1N1B4_2721 at 3061.034 kb on + strand, within Pf1N1B4_2721 at 3061.035 kb on - strand, within Pf1N1B4_2721 at 3061.035 kb on - strand, within Pf1N1B4_2721 at 3061.035 kb on - strand, within Pf1N1B4_2721 at 3061.235 kb on + strand at 3061.235 kb on + strand at 3061.236 kb on - strand at 3061.236 kb on - strand at 3061.236 kb on - strand at 3061.236 kb on - strand at 3061.236 kb on - strand at 3061.390 kb on + strand at 3061.412 kb on + strand at 3061.413 kb on - strand at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.809 kb on + strand, within Pf1N1B4_2722 at 3062.060 kb on + strand at 3062.061 kb on - strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.063 kb on - strand at 3062.063 kb on - strand at 3062.131 kb on + strand, within Pf1N1B4_2723 at 3062.132 kb on - strand, within Pf1N1B4_2723 at 3062.294 kb on + strand at 3062.294 kb on + strand at 3062.295 kb on - strand at 3062.309 kb on + strand at 3062.309 kb on + strand at 3062.310 kb on - strand at 3062.310 kb on - strand at 3062.310 kb on - strand at 3062.311 kb on + strand at 3062.311 kb on + strand at 3062.312 kb on - strand at 3062.312 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.5mM remove 3,059,220 + Pf1N1B4_2720 0.70 -0.2 3,059,220 + Pf1N1B4_2720 0.70 +1.3 3,059,220 + Pf1N1B4_2720 0.70 -2.6 3,059,221 - Pf1N1B4_2720 0.70 -0.7 3,059,221 - Pf1N1B4_2720 0.70 -0.1 3,059,287 - Pf1N1B4_2720 0.74 +0.4 3,059,377 + Pf1N1B4_2720 0.80 -1.1 3,059,377 + Pf1N1B4_2720 0.80 +3.7 3,059,377 + Pf1N1B4_2720 0.80 +1.0 3,059,377 + Pf1N1B4_2720 0.80 -1.0 3,059,378 - Pf1N1B4_2720 0.80 -0.8 3,059,378 - Pf1N1B4_2720 0.80 -1.4 3,059,378 - Pf1N1B4_2720 0.80 +0.5 3,059,566 + -0.3 3,059,566 + +0.9 3,059,566 + +1.6 3,059,567 - -1.2 3,059,640 + -1.5 3,059,640 + -1.9 3,059,640 + -0.7 3,059,641 - -1.4 3,059,733 + +0.5 3,059,734 - -1.9 3,059,734 - +1.4 3,059,734 - -1.7 3,059,734 - -1.1 3,059,795 + -1.9 3,059,796 - -0.8 3,059,796 - -1.0 3,059,908 + +4.8 3,059,999 + -1.2 3,060,000 - -1.6 3,060,072 - Pf1N1B4_2721 0.12 -1.4 3,060,072 - Pf1N1B4_2721 0.12 -1.6 3,060,072 - Pf1N1B4_2721 0.12 -1.5 3,060,072 - Pf1N1B4_2721 0.12 +1.3 3,060,072 - Pf1N1B4_2721 0.12 +0.2 3,060,072 - Pf1N1B4_2721 0.12 -1.7 3,060,110 + Pf1N1B4_2721 0.15 +2.3 3,060,111 - Pf1N1B4_2721 0.15 -1.9 3,060,137 + Pf1N1B4_2721 0.17 -0.5 3,060,137 + Pf1N1B4_2721 0.17 -0.9 3,060,137 + Pf1N1B4_2721 0.17 +0.9 3,060,137 + Pf1N1B4_2721 0.17 -1.9 3,060,138 - Pf1N1B4_2721 0.17 -1.2 3,060,138 - Pf1N1B4_2721 0.17 +0.1 3,060,138 - Pf1N1B4_2721 0.17 -0.7 3,060,138 - Pf1N1B4_2721 0.17 -0.1 3,060,176 + -1.5 3,060,176 + Pf1N1B4_2721 0.19 -2.1 3,060,176 + Pf1N1B4_2721 0.19 -0.5 3,060,176 + Pf1N1B4_2721 0.19 -1.3 3,060,177 - Pf1N1B4_2721 0.19 +0.7 3,060,177 - Pf1N1B4_2721 0.19 +0.9 3,060,263 + Pf1N1B4_2721 0.25 +1.0 3,060,337 + Pf1N1B4_2721 0.30 -1.2 3,060,380 + Pf1N1B4_2721 0.33 -2.6 3,060,381 - Pf1N1B4_2721 0.33 -0.9 3,060,464 + Pf1N1B4_2721 0.39 +1.6 3,060,465 - Pf1N1B4_2721 0.39 -1.4 3,060,465 - Pf1N1B4_2721 0.39 -1.0 3,060,465 - Pf1N1B4_2721 0.39 -1.9 3,060,515 + Pf1N1B4_2721 0.42 +0.0 3,060,515 + Pf1N1B4_2721 0.42 -1.7 3,060,515 + Pf1N1B4_2721 0.42 -0.8 3,060,516 - Pf1N1B4_2721 0.42 +0.5 3,060,689 + -0.3 3,060,854 + Pf1N1B4_2721 0.66 -0.9 3,060,854 + Pf1N1B4_2721 0.66 -1.1 3,060,872 + Pf1N1B4_2721 0.67 -0.1 3,060,872 + Pf1N1B4_2721 0.67 -1.0 3,060,872 + Pf1N1B4_2721 0.67 -1.5 3,060,872 + Pf1N1B4_2721 0.67 +1.7 3,060,872 + Pf1N1B4_2721 0.67 +0.1 3,060,872 + Pf1N1B4_2721 0.67 -1.7 3,060,872 + Pf1N1B4_2721 0.67 -1.2 3,060,873 - Pf1N1B4_2721 0.67 +1.0 3,060,873 - Pf1N1B4_2721 0.67 -0.2 3,060,873 - Pf1N1B4_2721 0.67 -4.3 3,060,873 - Pf1N1B4_2721 0.67 -0.4 3,061,034 + Pf1N1B4_2721 0.78 -1.9 3,061,034 + Pf1N1B4_2721 0.78 -2.6 3,061,034 + Pf1N1B4_2721 0.78 +1.0 3,061,034 + Pf1N1B4_2721 0.78 -2.1 3,061,035 - Pf1N1B4_2721 0.78 -0.1 3,061,035 - Pf1N1B4_2721 0.78 -0.7 3,061,035 - Pf1N1B4_2721 0.78 +2.3 3,061,235 + +2.1 3,061,235 + -1.8 3,061,236 - +2.6 3,061,236 - -2.2 3,061,236 - +1.6 3,061,236 - -1.1 3,061,236 - -1.7 3,061,390 + -2.1 3,061,412 + -1.4 3,061,413 - +0.7 3,061,732 + Pf1N1B4_2722 0.48 -1.6 3,061,732 + Pf1N1B4_2722 0.48 +1.1 3,061,732 + Pf1N1B4_2722 0.48 -3.0 3,061,732 + Pf1N1B4_2722 0.48 -1.7 3,061,733 - Pf1N1B4_2722 0.48 +3.6 3,061,733 - Pf1N1B4_2722 0.48 +0.8 3,061,733 - Pf1N1B4_2722 0.48 +0.4 3,061,733 - Pf1N1B4_2722 0.48 -0.8 3,061,733 - Pf1N1B4_2722 0.48 -1.8 3,061,733 - Pf1N1B4_2722 0.48 +2.0 3,061,809 + Pf1N1B4_2722 0.60 +0.5 3,062,060 + -1.3 3,062,061 - -0.3 3,062,062 + +1.5 3,062,062 + +2.2 3,062,062 + -1.5 3,062,062 + +2.1 3,062,062 + +3.0 3,062,062 + +1.1 3,062,063 - -2.1 3,062,063 - -0.7 3,062,131 + Pf1N1B4_2723 0.34 +1.2 3,062,132 - Pf1N1B4_2723 0.35 -0.6 3,062,294 + +1.0 3,062,294 + -1.6 3,062,295 - -2.5 3,062,309 + +2.3 3,062,309 + -0.5 3,062,310 - -1.6 3,062,310 - -1.3 3,062,310 - -1.2 3,062,311 + +1.5 3,062,311 + -1.7 3,062,312 - +0.9 3,062,312 - +0.5
Or see this region's nucleotide sequence