Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2485

Experiment: carbon source Sucrose 0.5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2483 and Pf1N1B4_2484 are separated by 24 nucleotidesPf1N1B4_2484 and Pf1N1B4_2485 overlap by 43 nucleotidesPf1N1B4_2485 and Pf1N1B4_2486 are separated by 114 nucleotidesPf1N1B4_2486 and Pf1N1B4_2487 overlap by 33 nucleotides Pf1N1B4_2483 - DNA-binding domain of ModE / Molybdate-binding domain of ModE, at 2,818,158 to 2,818,922 _2483 Pf1N1B4_2484 - hypothetical protein, at 2,818,947 to 2,819,150 _2484 Pf1N1B4_2485 - Competence protein F homolog, phosphoribosyltransferase domain; protein YhgH required for utilization of DNA as sole source of carbon and energy, at 2,819,108 to 2,819,743 _2485 Pf1N1B4_2486 - hypothetical protein, at 2,819,858 to 2,819,971 _2486 Pf1N1B4_2487 - Biotin synthase (EC 2.8.1.6), at 2,819,939 to 2,820,994 _2487 Position (kb) 2819 2820Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2818.134 kb on + strandat 2818.134 kb on + strandat 2818.135 kb on - strandat 2818.169 kb on + strandat 2818.423 kb on - strand, within Pf1N1B4_2483at 2818.670 kb on - strand, within Pf1N1B4_2483at 2818.670 kb on - strand, within Pf1N1B4_2483at 2818.801 kb on + strand, within Pf1N1B4_2483at 2818.801 kb on + strandat 2818.801 kb on + strand, within Pf1N1B4_2483at 2818.801 kb on + strand, within Pf1N1B4_2483at 2818.801 kb on + strand, within Pf1N1B4_2483at 2818.801 kb on + strand, within Pf1N1B4_2483at 2818.802 kb on - strand, within Pf1N1B4_2483at 2818.802 kb on - strand, within Pf1N1B4_2483at 2818.802 kb on - strand, within Pf1N1B4_2483at 2818.910 kb on + strandat 2818.910 kb on + strandat 2818.911 kb on - strandat 2818.911 kb on - strandat 2819.033 kb on + strand, within Pf1N1B4_2484at 2819.034 kb on - strand, within Pf1N1B4_2484at 2819.104 kb on + strand, within Pf1N1B4_2484at 2819.104 kb on + strand, within Pf1N1B4_2484at 2819.104 kb on + strandat 2819.104 kb on + strand, within Pf1N1B4_2484at 2819.105 kb on - strand, within Pf1N1B4_2484at 2819.105 kb on - strand, within Pf1N1B4_2484at 2819.148 kb on + strandat 2819.149 kb on - strandat 2819.604 kb on + strand, within Pf1N1B4_2485at 2819.604 kb on + strand, within Pf1N1B4_2485at 2819.604 kb on + strand, within Pf1N1B4_2485at 2819.605 kb on - strand, within Pf1N1B4_2485at 2819.605 kb on - strand, within Pf1N1B4_2485at 2819.744 kb on - strandat 2819.783 kb on + strandat 2819.784 kb on - strandat 2819.784 kb on - strandat 2819.814 kb on + strandat 2819.826 kb on - strandat 2819.859 kb on + strandat 2819.863 kb on + strandat 2819.863 kb on + strandat 2819.863 kb on + strandat 2819.863 kb on + strandat 2819.863 kb on + strandat 2819.863 kb on + strandat 2819.863 kb on + strandat 2819.863 kb on + strandat 2819.863 kb on + strandat 2819.864 kb on - strandat 2819.864 kb on - strandat 2819.894 kb on - strand, within Pf1N1B4_2486at 2819.922 kb on + strand, within Pf1N1B4_2486at 2819.923 kb on - strand, within Pf1N1B4_2486at 2819.923 kb on - strand, within Pf1N1B4_2486at 2819.923 kb on - strand, within Pf1N1B4_2486at 2819.923 kb on - strand, within Pf1N1B4_2486at 2820.131 kb on + strand, within Pf1N1B4_2487at 2820.132 kb on - strand, within Pf1N1B4_2487at 2820.152 kb on + strand, within Pf1N1B4_2487at 2820.152 kb on + strand, within Pf1N1B4_2487at 2820.152 kb on + strand, within Pf1N1B4_2487at 2820.153 kb on - strand, within Pf1N1B4_2487at 2820.153 kb on - strand, within Pf1N1B4_2487at 2820.153 kb on - strand, within Pf1N1B4_2487at 2820.153 kb on - strand, within Pf1N1B4_2487at 2820.290 kb on + strand, within Pf1N1B4_2487at 2820.291 kb on - strand, within Pf1N1B4_2487at 2820.401 kb on + strand, within Pf1N1B4_2487at 2820.401 kb on + strand, within Pf1N1B4_2487at 2820.402 kb on - strandat 2820.404 kb on + strand, within Pf1N1B4_2487at 2820.404 kb on + strand, within Pf1N1B4_2487at 2820.404 kb on + strand, within Pf1N1B4_2487at 2820.404 kb on + strand, within Pf1N1B4_2487at 2820.404 kb on + strand, within Pf1N1B4_2487at 2820.404 kb on + strand, within Pf1N1B4_2487at 2820.405 kb on - strand, within Pf1N1B4_2487at 2820.405 kb on - strand, within Pf1N1B4_2487at 2820.405 kb on - strand, within Pf1N1B4_2487at 2820.405 kb on - strand, within Pf1N1B4_2487at 2820.405 kb on - strand, within Pf1N1B4_2487at 2820.437 kb on + strand, within Pf1N1B4_2487at 2820.437 kb on + strand, within Pf1N1B4_2487at 2820.437 kb on + strand, within Pf1N1B4_2487at 2820.438 kb on - strand, within Pf1N1B4_2487at 2820.460 kb on + strand, within Pf1N1B4_2487at 2820.460 kb on + strand, within Pf1N1B4_2487at 2820.460 kb on + strand, within Pf1N1B4_2487at 2820.460 kb on + strand, within Pf1N1B4_2487at 2820.464 kb on + strand, within Pf1N1B4_2487at 2820.464 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.491 kb on + strand, within Pf1N1B4_2487at 2820.492 kb on - strand, within Pf1N1B4_2487

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source Sucrose 0.5mM
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2,818,134 + +0.9
2,818,134 + -0.4
2,818,135 - +1.2
2,818,169 + +2.8
2,818,423 - Pf1N1B4_2483 0.35 +1.9
2,818,670 - Pf1N1B4_2483 0.67 +0.9
2,818,670 - Pf1N1B4_2483 0.67 +0.7
2,818,801 + Pf1N1B4_2483 0.84 +0.0
2,818,801 + -0.6
2,818,801 + Pf1N1B4_2483 0.84 -2.2
2,818,801 + Pf1N1B4_2483 0.84 -2.3
2,818,801 + Pf1N1B4_2483 0.84 +1.6
2,818,801 + Pf1N1B4_2483 0.84 +1.2
2,818,802 - Pf1N1B4_2483 0.84 +2.1
2,818,802 - Pf1N1B4_2483 0.84 -1.8
2,818,802 - Pf1N1B4_2483 0.84 -1.4
2,818,910 + -1.7
2,818,910 + +3.9
2,818,911 - -2.6
2,818,911 - +1.7
2,819,033 + Pf1N1B4_2484 0.42 -1.0
2,819,034 - Pf1N1B4_2484 0.43 +0.6
2,819,104 + Pf1N1B4_2484 0.77 -2.4
2,819,104 + Pf1N1B4_2484 0.77 +1.2
2,819,104 + -0.6
2,819,104 + Pf1N1B4_2484 0.77 +2.0
2,819,105 - Pf1N1B4_2484 0.77 +2.5
2,819,105 - Pf1N1B4_2484 0.77 +0.4
2,819,148 + +0.8
2,819,149 - -2.0
2,819,604 + Pf1N1B4_2485 0.78 -0.6
2,819,604 + Pf1N1B4_2485 0.78 +2.2
2,819,604 + Pf1N1B4_2485 0.78 +1.9
2,819,605 - Pf1N1B4_2485 0.78 -0.5
2,819,605 - Pf1N1B4_2485 0.78 +0.0
2,819,744 - -1.8
2,819,783 + -1.1
2,819,784 - +0.7
2,819,784 - -0.7
2,819,814 + +1.3
2,819,826 - -1.7
2,819,859 + +1.0
2,819,863 + +0.9
2,819,863 + -1.7
2,819,863 + -1.6
2,819,863 + +0.6
2,819,863 + -2.7
2,819,863 + +0.0
2,819,863 + -0.7
2,819,863 + -1.5
2,819,863 + -2.3
2,819,864 - -0.7
2,819,864 - -0.4
2,819,894 - Pf1N1B4_2486 0.32 +0.4
2,819,922 + Pf1N1B4_2486 0.56 +0.4
2,819,923 - Pf1N1B4_2486 0.57 -2.0
2,819,923 - Pf1N1B4_2486 0.57 -0.4
2,819,923 - Pf1N1B4_2486 0.57 -1.0
2,819,923 - Pf1N1B4_2486 0.57 -1.9
2,820,131 + Pf1N1B4_2487 0.18 -0.4
2,820,132 - Pf1N1B4_2487 0.18 -1.2
2,820,152 + Pf1N1B4_2487 0.20 -1.0
2,820,152 + Pf1N1B4_2487 0.20 -1.5
2,820,152 + Pf1N1B4_2487 0.20 +0.5
2,820,153 - Pf1N1B4_2487 0.20 -1.2
2,820,153 - Pf1N1B4_2487 0.20 -0.7
2,820,153 - Pf1N1B4_2487 0.20 +0.4
2,820,153 - Pf1N1B4_2487 0.20 +0.2
2,820,290 + Pf1N1B4_2487 0.33 +0.3
2,820,291 - Pf1N1B4_2487 0.33 -1.7
2,820,401 + Pf1N1B4_2487 0.44 -0.0
2,820,401 + Pf1N1B4_2487 0.44 -0.0
2,820,402 - +0.8
2,820,404 + Pf1N1B4_2487 0.44 -0.4
2,820,404 + Pf1N1B4_2487 0.44 -1.0
2,820,404 + Pf1N1B4_2487 0.44 -0.0
2,820,404 + Pf1N1B4_2487 0.44 -1.0
2,820,404 + Pf1N1B4_2487 0.44 -1.0
2,820,404 + Pf1N1B4_2487 0.44 -0.4
2,820,405 - Pf1N1B4_2487 0.44 -1.1
2,820,405 - Pf1N1B4_2487 0.44 -2.6
2,820,405 - Pf1N1B4_2487 0.44 -0.6
2,820,405 - Pf1N1B4_2487 0.44 -0.9
2,820,405 - Pf1N1B4_2487 0.44 -1.2
2,820,437 + Pf1N1B4_2487 0.47 -1.1
2,820,437 + Pf1N1B4_2487 0.47 -0.6
2,820,437 + Pf1N1B4_2487 0.47 -1.5
2,820,438 - Pf1N1B4_2487 0.47 -0.7
2,820,460 + Pf1N1B4_2487 0.49 -0.0
2,820,460 + Pf1N1B4_2487 0.49 +0.6
2,820,460 + Pf1N1B4_2487 0.49 -0.5
2,820,460 + Pf1N1B4_2487 0.49 +1.5
2,820,464 + Pf1N1B4_2487 0.50 -1.7
2,820,464 + Pf1N1B4_2487 0.50 +2.1
2,820,491 + Pf1N1B4_2487 0.52 -1.1
2,820,491 + Pf1N1B4_2487 0.52 -2.4
2,820,491 + Pf1N1B4_2487 0.52 -3.7
2,820,491 + Pf1N1B4_2487 0.52 -2.1
2,820,491 + Pf1N1B4_2487 0.52 -2.9
2,820,491 + Pf1N1B4_2487 0.52 -2.0
2,820,491 + Pf1N1B4_2487 0.52 -2.0
2,820,492 - Pf1N1B4_2487 0.52 -0.9

Or see this region's nucleotide sequence