Experiment: carbon source Sucrose 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2483 and Pf1N1B4_2484 are separated by 24 nucleotides Pf1N1B4_2484 and Pf1N1B4_2485 overlap by 43 nucleotides Pf1N1B4_2485 and Pf1N1B4_2486 are separated by 114 nucleotides Pf1N1B4_2486 and Pf1N1B4_2487 overlap by 33 nucleotides
Pf1N1B4_2483 - DNA-binding domain of ModE / Molybdate-binding domain of ModE, at 2,818,158 to 2,818,922
_2483
Pf1N1B4_2484 - hypothetical protein, at 2,818,947 to 2,819,150
_2484
Pf1N1B4_2485 - Competence protein F homolog, phosphoribosyltransferase domain; protein YhgH required for utilization of DNA as sole source of carbon and energy, at 2,819,108 to 2,819,743
_2485
Pf1N1B4_2486 - hypothetical protein, at 2,819,858 to 2,819,971
_2486
Pf1N1B4_2487 - Biotin synthase (EC 2.8.1.6), at 2,819,939 to 2,820,994
_2487
Position (kb)
2819
2820 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2818.134 kb on + strand at 2818.134 kb on + strand at 2818.135 kb on - strand at 2818.169 kb on + strand at 2818.423 kb on - strand, within Pf1N1B4_2483 at 2818.670 kb on - strand, within Pf1N1B4_2483 at 2818.670 kb on - strand, within Pf1N1B4_2483 at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.801 kb on + strand at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.801 kb on + strand, within Pf1N1B4_2483 at 2818.802 kb on - strand, within Pf1N1B4_2483 at 2818.802 kb on - strand, within Pf1N1B4_2483 at 2818.802 kb on - strand, within Pf1N1B4_2483 at 2818.910 kb on + strand at 2818.910 kb on + strand at 2818.911 kb on - strand at 2818.911 kb on - strand at 2819.033 kb on + strand, within Pf1N1B4_2484 at 2819.034 kb on - strand, within Pf1N1B4_2484 at 2819.104 kb on + strand, within Pf1N1B4_2484 at 2819.104 kb on + strand, within Pf1N1B4_2484 at 2819.104 kb on + strand at 2819.104 kb on + strand, within Pf1N1B4_2484 at 2819.105 kb on - strand, within Pf1N1B4_2484 at 2819.105 kb on - strand, within Pf1N1B4_2484 at 2819.148 kb on + strand at 2819.149 kb on - strand at 2819.604 kb on + strand, within Pf1N1B4_2485 at 2819.604 kb on + strand, within Pf1N1B4_2485 at 2819.604 kb on + strand, within Pf1N1B4_2485 at 2819.605 kb on - strand, within Pf1N1B4_2485 at 2819.605 kb on - strand, within Pf1N1B4_2485 at 2819.744 kb on - strand at 2819.783 kb on + strand at 2819.784 kb on - strand at 2819.784 kb on - strand at 2819.814 kb on + strand at 2819.826 kb on - strand at 2819.859 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.863 kb on + strand at 2819.864 kb on - strand at 2819.864 kb on - strand at 2819.894 kb on - strand, within Pf1N1B4_2486 at 2819.922 kb on + strand, within Pf1N1B4_2486 at 2819.923 kb on - strand, within Pf1N1B4_2486 at 2819.923 kb on - strand, within Pf1N1B4_2486 at 2819.923 kb on - strand, within Pf1N1B4_2486 at 2819.923 kb on - strand, within Pf1N1B4_2486 at 2820.131 kb on + strand, within Pf1N1B4_2487 at 2820.132 kb on - strand, within Pf1N1B4_2487 at 2820.152 kb on + strand, within Pf1N1B4_2487 at 2820.152 kb on + strand, within Pf1N1B4_2487 at 2820.152 kb on + strand, within Pf1N1B4_2487 at 2820.153 kb on - strand, within Pf1N1B4_2487 at 2820.153 kb on - strand, within Pf1N1B4_2487 at 2820.153 kb on - strand, within Pf1N1B4_2487 at 2820.153 kb on - strand, within Pf1N1B4_2487 at 2820.290 kb on + strand, within Pf1N1B4_2487 at 2820.291 kb on - strand, within Pf1N1B4_2487 at 2820.401 kb on + strand, within Pf1N1B4_2487 at 2820.401 kb on + strand, within Pf1N1B4_2487 at 2820.402 kb on - strand at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.404 kb on + strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.405 kb on - strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.437 kb on + strand, within Pf1N1B4_2487 at 2820.438 kb on - strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.460 kb on + strand, within Pf1N1B4_2487 at 2820.464 kb on + strand, within Pf1N1B4_2487 at 2820.464 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.491 kb on + strand, within Pf1N1B4_2487 at 2820.492 kb on - strand, within Pf1N1B4_2487
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.5mM remove 2,818,134 + +0.9 2,818,134 + -0.4 2,818,135 - +1.2 2,818,169 + +2.8 2,818,423 - Pf1N1B4_2483 0.35 +1.9 2,818,670 - Pf1N1B4_2483 0.67 +0.9 2,818,670 - Pf1N1B4_2483 0.67 +0.7 2,818,801 + Pf1N1B4_2483 0.84 +0.0 2,818,801 + -0.6 2,818,801 + Pf1N1B4_2483 0.84 -2.2 2,818,801 + Pf1N1B4_2483 0.84 -2.3 2,818,801 + Pf1N1B4_2483 0.84 +1.6 2,818,801 + Pf1N1B4_2483 0.84 +1.2 2,818,802 - Pf1N1B4_2483 0.84 +2.1 2,818,802 - Pf1N1B4_2483 0.84 -1.8 2,818,802 - Pf1N1B4_2483 0.84 -1.4 2,818,910 + -1.7 2,818,910 + +3.9 2,818,911 - -2.6 2,818,911 - +1.7 2,819,033 + Pf1N1B4_2484 0.42 -1.0 2,819,034 - Pf1N1B4_2484 0.43 +0.6 2,819,104 + Pf1N1B4_2484 0.77 -2.4 2,819,104 + Pf1N1B4_2484 0.77 +1.2 2,819,104 + -0.6 2,819,104 + Pf1N1B4_2484 0.77 +2.0 2,819,105 - Pf1N1B4_2484 0.77 +2.5 2,819,105 - Pf1N1B4_2484 0.77 +0.4 2,819,148 + +0.8 2,819,149 - -2.0 2,819,604 + Pf1N1B4_2485 0.78 -0.6 2,819,604 + Pf1N1B4_2485 0.78 +2.2 2,819,604 + Pf1N1B4_2485 0.78 +1.9 2,819,605 - Pf1N1B4_2485 0.78 -0.5 2,819,605 - Pf1N1B4_2485 0.78 +0.0 2,819,744 - -1.8 2,819,783 + -1.1 2,819,784 - +0.7 2,819,784 - -0.7 2,819,814 + +1.3 2,819,826 - -1.7 2,819,859 + +1.0 2,819,863 + +0.9 2,819,863 + -1.7 2,819,863 + -1.6 2,819,863 + +0.6 2,819,863 + -2.7 2,819,863 + +0.0 2,819,863 + -0.7 2,819,863 + -1.5 2,819,863 + -2.3 2,819,864 - -0.7 2,819,864 - -0.4 2,819,894 - Pf1N1B4_2486 0.32 +0.4 2,819,922 + Pf1N1B4_2486 0.56 +0.4 2,819,923 - Pf1N1B4_2486 0.57 -2.0 2,819,923 - Pf1N1B4_2486 0.57 -0.4 2,819,923 - Pf1N1B4_2486 0.57 -1.0 2,819,923 - Pf1N1B4_2486 0.57 -1.9 2,820,131 + Pf1N1B4_2487 0.18 -0.4 2,820,132 - Pf1N1B4_2487 0.18 -1.2 2,820,152 + Pf1N1B4_2487 0.20 -1.0 2,820,152 + Pf1N1B4_2487 0.20 -1.5 2,820,152 + Pf1N1B4_2487 0.20 +0.5 2,820,153 - Pf1N1B4_2487 0.20 -1.2 2,820,153 - Pf1N1B4_2487 0.20 -0.7 2,820,153 - Pf1N1B4_2487 0.20 +0.4 2,820,153 - Pf1N1B4_2487 0.20 +0.2 2,820,290 + Pf1N1B4_2487 0.33 +0.3 2,820,291 - Pf1N1B4_2487 0.33 -1.7 2,820,401 + Pf1N1B4_2487 0.44 -0.0 2,820,401 + Pf1N1B4_2487 0.44 -0.0 2,820,402 - +0.8 2,820,404 + Pf1N1B4_2487 0.44 -0.4 2,820,404 + Pf1N1B4_2487 0.44 -1.0 2,820,404 + Pf1N1B4_2487 0.44 -0.0 2,820,404 + Pf1N1B4_2487 0.44 -1.0 2,820,404 + Pf1N1B4_2487 0.44 -1.0 2,820,404 + Pf1N1B4_2487 0.44 -0.4 2,820,405 - Pf1N1B4_2487 0.44 -1.1 2,820,405 - Pf1N1B4_2487 0.44 -2.6 2,820,405 - Pf1N1B4_2487 0.44 -0.6 2,820,405 - Pf1N1B4_2487 0.44 -0.9 2,820,405 - Pf1N1B4_2487 0.44 -1.2 2,820,437 + Pf1N1B4_2487 0.47 -1.1 2,820,437 + Pf1N1B4_2487 0.47 -0.6 2,820,437 + Pf1N1B4_2487 0.47 -1.5 2,820,438 - Pf1N1B4_2487 0.47 -0.7 2,820,460 + Pf1N1B4_2487 0.49 -0.0 2,820,460 + Pf1N1B4_2487 0.49 +0.6 2,820,460 + Pf1N1B4_2487 0.49 -0.5 2,820,460 + Pf1N1B4_2487 0.49 +1.5 2,820,464 + Pf1N1B4_2487 0.50 -1.7 2,820,464 + Pf1N1B4_2487 0.50 +2.1 2,820,491 + Pf1N1B4_2487 0.52 -1.1 2,820,491 + Pf1N1B4_2487 0.52 -2.4 2,820,491 + Pf1N1B4_2487 0.52 -3.7 2,820,491 + Pf1N1B4_2487 0.52 -2.1 2,820,491 + Pf1N1B4_2487 0.52 -2.9 2,820,491 + Pf1N1B4_2487 0.52 -2.0 2,820,491 + Pf1N1B4_2487 0.52 -2.0 2,820,492 - Pf1N1B4_2487 0.52 -0.9
Or see this region's nucleotide sequence