Experiment: carbon source Sucrose 0.5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_213 and Pf1N1B4_214 are separated by 202 nucleotides Pf1N1B4_214 and Pf1N1B4_215 overlap by 19 nucleotides
Pf1N1B4_213 - Beta-ketoadipate enol-lactone hydrolase (EC 3.1.1.24), at 261,393 to 262,205
_213
Pf1N1B4_214 - Pyoverdine chromophore precursor synthetase PvdL, at 262,408 to 267,885
_214
Pf1N1B4_215 - Pyoverdine chromophore precursor synthetase PvdL, at 267,867 to 275,390
_215
Position (kb) 262
263
264
265
266
267
268 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 261.462 kb on - strand at 261.462 kb on - strand at 261.487 kb on + strand, within Pf1N1B4_213 at 261.488 kb on - strand, within Pf1N1B4_213 at 261.623 kb on + strand, within Pf1N1B4_213 at 261.749 kb on + strand, within Pf1N1B4_213 at 261.750 kb on - strand, within Pf1N1B4_213 at 261.750 kb on - strand, within Pf1N1B4_213 at 261.811 kb on + strand, within Pf1N1B4_213 at 261.811 kb on + strand, within Pf1N1B4_213 at 261.811 kb on + strand, within Pf1N1B4_213 at 261.812 kb on - strand, within Pf1N1B4_213 at 261.812 kb on - strand, within Pf1N1B4_213 at 261.812 kb on - strand at 261.812 kb on - strand, within Pf1N1B4_213 at 261.814 kb on + strand, within Pf1N1B4_213 at 261.847 kb on + strand, within Pf1N1B4_213 at 261.847 kb on + strand, within Pf1N1B4_213 at 261.848 kb on - strand, within Pf1N1B4_213 at 262.106 kb on + strand at 262.106 kb on + strand, within Pf1N1B4_213 at 262.123 kb on + strand, within Pf1N1B4_213 at 262.123 kb on + strand, within Pf1N1B4_213 at 262.164 kb on + strand at 262.164 kb on + strand at 262.164 kb on + strand at 262.164 kb on + strand at 262.164 kb on + strand at 262.164 kb on + strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.165 kb on - strand at 262.168 kb on + strand at 262.168 kb on + strand at 262.168 kb on + strand at 262.169 kb on - strand at 262.169 kb on - strand at 262.226 kb on + strand at 262.227 kb on - strand at 262.227 kb on - strand at 262.227 kb on - strand at 262.230 kb on - strand at 262.289 kb on + strand at 262.289 kb on + strand at 262.289 kb on + strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.290 kb on - strand at 262.409 kb on + strand at 262.410 kb on - strand at 262.455 kb on + strand at 262.494 kb on - strand at 262.668 kb on + strand at 262.823 kb on + strand at 262.823 kb on + strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.824 kb on - strand at 262.908 kb on - strand at 262.908 kb on - strand at 262.908 kb on - strand at 263.102 kb on + strand, within Pf1N1B4_214 at 263.175 kb on - strand, within Pf1N1B4_214 at 263.175 kb on - strand, within Pf1N1B4_214 at 263.175 kb on - strand, within Pf1N1B4_214 at 263.703 kb on - strand, within Pf1N1B4_214 at 263.787 kb on - strand, within Pf1N1B4_214 at 263.787 kb on - strand, within Pf1N1B4_214 at 263.790 kb on + strand, within Pf1N1B4_214 at 263.790 kb on + strand, within Pf1N1B4_214 at 263.819 kb on + strand, within Pf1N1B4_214 at 263.820 kb on - strand, within Pf1N1B4_214 at 263.820 kb on - strand, within Pf1N1B4_214 at 264.019 kb on + strand, within Pf1N1B4_214 at 264.020 kb on - strand, within Pf1N1B4_214 at 264.023 kb on + strand, within Pf1N1B4_214 at 264.171 kb on - strand, within Pf1N1B4_214 at 264.171 kb on - strand at 264.248 kb on + strand, within Pf1N1B4_214 at 264.248 kb on + strand, within Pf1N1B4_214 at 264.248 kb on + strand, within Pf1N1B4_214 at 264.248 kb on + strand, within Pf1N1B4_214 at 264.248 kb on + strand, within Pf1N1B4_214 at 264.248 kb on + strand at 264.248 kb on + strand at 264.248 kb on + strand, within Pf1N1B4_214 at 264.248 kb on + strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.249 kb on - strand, within Pf1N1B4_214 at 264.425 kb on + strand, within Pf1N1B4_214 at 264.426 kb on - strand, within Pf1N1B4_214 at 264.438 kb on - strand at 264.438 kb on - strand, within Pf1N1B4_214 at 264.612 kb on + strand, within Pf1N1B4_214 at 264.653 kb on + strand, within Pf1N1B4_214 at 264.653 kb on + strand at 264.654 kb on - strand, within Pf1N1B4_214 at 264.654 kb on - strand, within Pf1N1B4_214 at 264.660 kb on - strand, within Pf1N1B4_214 at 264.963 kb on + strand, within Pf1N1B4_214 at 265.121 kb on + strand, within Pf1N1B4_214 at 265.122 kb on - strand, within Pf1N1B4_214 at 265.181 kb on + strand, within Pf1N1B4_214 at 265.181 kb on + strand at 265.182 kb on - strand, within Pf1N1B4_214 at 265.182 kb on - strand, within Pf1N1B4_214 at 265.403 kb on + strand, within Pf1N1B4_214 at 265.404 kb on - strand, within Pf1N1B4_214 at 265.571 kb on + strand, within Pf1N1B4_214 at 265.571 kb on + strand, within Pf1N1B4_214 at 265.571 kb on + strand, within Pf1N1B4_214 at 265.572 kb on - strand at 265.572 kb on - strand, within Pf1N1B4_214 at 265.572 kb on - strand at 265.572 kb on - strand, within Pf1N1B4_214 at 265.574 kb on + strand, within Pf1N1B4_214 at 265.784 kb on + strand, within Pf1N1B4_214 at 265.865 kb on + strand, within Pf1N1B4_214 at 265.992 kb on - strand, within Pf1N1B4_214 at 265.992 kb on - strand at 266.278 kb on + strand, within Pf1N1B4_214 at 266.279 kb on - strand, within Pf1N1B4_214 at 266.279 kb on - strand, within Pf1N1B4_214 at 266.279 kb on - strand, within Pf1N1B4_214 at 266.280 kb on - strand, within Pf1N1B4_214 at 266.566 kb on + strand, within Pf1N1B4_214 at 267.293 kb on + strand, within Pf1N1B4_214 at 267.293 kb on + strand, within Pf1N1B4_214 at 267.293 kb on + strand, within Pf1N1B4_214 at 267.294 kb on - strand, within Pf1N1B4_214 at 267.404 kb on + strand at 267.404 kb on + strand at 267.405 kb on - strand at 267.644 kb on + strand at 267.795 kb on - strand at 267.795 kb on - strand at 267.848 kb on + strand at 267.848 kb on + strand at 267.848 kb on + strand at 267.849 kb on - strand at 267.849 kb on - strand at 267.959 kb on - strand at 268.102 kb on + strand at 268.102 kb on + strand at 268.102 kb on + strand at 268.134 kb on - strand at 268.258 kb on + strand at 268.259 kb on - strand at 268.259 kb on - strand at 268.381 kb on + strand at 268.390 kb on + strand at 268.416 kb on - strand at 268.564 kb on + strand at 268.564 kb on + strand at 268.564 kb on + strand at 268.565 kb on - strand at 268.565 kb on - strand at 268.606 kb on + strand at 268.607 kb on - strand at 268.607 kb on - strand at 268.607 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 0.5mM remove 261,462 - -1.1 261,462 - -1.1 261,487 + Pf1N1B4_213 0.12 -0.1 261,488 - Pf1N1B4_213 0.12 -1.6 261,623 + Pf1N1B4_213 0.28 -2.0 261,749 + Pf1N1B4_213 0.44 +1.5 261,750 - Pf1N1B4_213 0.44 -2.7 261,750 - Pf1N1B4_213 0.44 +0.4 261,811 + Pf1N1B4_213 0.51 +1.0 261,811 + Pf1N1B4_213 0.51 -1.9 261,811 + Pf1N1B4_213 0.51 -0.6 261,812 - Pf1N1B4_213 0.52 -1.7 261,812 - Pf1N1B4_213 0.52 -2.4 261,812 - +2.5 261,812 - Pf1N1B4_213 0.52 -1.2 261,814 + Pf1N1B4_213 0.52 -0.7 261,847 + Pf1N1B4_213 0.56 -2.5 261,847 + Pf1N1B4_213 0.56 -2.4 261,848 - Pf1N1B4_213 0.56 +1.5 262,106 + +1.2 262,106 + Pf1N1B4_213 0.88 -0.9 262,123 + Pf1N1B4_213 0.90 -2.2 262,123 + Pf1N1B4_213 0.90 -0.8 262,164 + +1.4 262,164 + -1.5 262,164 + -1.0 262,164 + -1.9 262,164 + -1.4 262,164 + -2.5 262,165 - -1.1 262,165 - -1.7 262,165 - -3.0 262,165 - +2.9 262,165 - +0.5 262,165 - +0.3 262,168 + +3.2 262,168 + -0.8 262,168 + +3.6 262,169 - -1.0 262,169 - -0.6 262,226 + +0.1 262,227 - +0.5 262,227 - +1.5 262,227 - +1.9 262,230 - -0.1 262,289 + +1.0 262,289 + -3.0 262,289 + +2.3 262,290 - -2.0 262,290 - -1.8 262,290 - +2.6 262,290 - +0.8 262,290 - -0.1 262,290 - -1.2 262,409 + +1.3 262,410 - +0.4 262,455 + -1.6 262,494 - +2.2 262,668 + +1.2 262,823 + -1.4 262,823 + -1.2 262,824 - -1.2 262,824 - +0.8 262,824 - +2.1 262,824 - +0.4 262,824 - -1.9 262,824 - +1.5 262,908 - -2.1 262,908 - -1.2 262,908 - +2.6 263,102 + Pf1N1B4_214 0.13 +1.4 263,175 - Pf1N1B4_214 0.14 -1.8 263,175 - Pf1N1B4_214 0.14 -1.0 263,175 - Pf1N1B4_214 0.14 -2.3 263,703 - Pf1N1B4_214 0.24 -0.5 263,787 - Pf1N1B4_214 0.25 -1.8 263,787 - Pf1N1B4_214 0.25 +1.3 263,790 + Pf1N1B4_214 0.25 +1.7 263,790 + Pf1N1B4_214 0.25 -0.8 263,819 + Pf1N1B4_214 0.26 -0.3 263,820 - Pf1N1B4_214 0.26 -2.7 263,820 - Pf1N1B4_214 0.26 -1.4 264,019 + Pf1N1B4_214 0.29 -2.1 264,020 - Pf1N1B4_214 0.29 -0.8 264,023 + Pf1N1B4_214 0.29 +1.9 264,171 - Pf1N1B4_214 0.32 +1.0 264,171 - -1.8 264,248 + Pf1N1B4_214 0.34 -1.4 264,248 + Pf1N1B4_214 0.34 -1.5 264,248 + Pf1N1B4_214 0.34 -3.3 264,248 + Pf1N1B4_214 0.34 +2.5 264,248 + Pf1N1B4_214 0.34 -0.8 264,248 + +0.9 264,248 + +0.7 264,248 + Pf1N1B4_214 0.34 +0.9 264,248 + Pf1N1B4_214 0.34 -1.2 264,249 - Pf1N1B4_214 0.34 +1.5 264,249 - Pf1N1B4_214 0.34 -1.5 264,249 - Pf1N1B4_214 0.34 -2.6 264,249 - Pf1N1B4_214 0.34 -0.4 264,249 - Pf1N1B4_214 0.34 +1.6 264,249 - Pf1N1B4_214 0.34 +1.6 264,249 - Pf1N1B4_214 0.34 -1.6 264,249 - -1.1 264,249 - Pf1N1B4_214 0.34 -1.9 264,249 - Pf1N1B4_214 0.34 +2.2 264,249 - Pf1N1B4_214 0.34 +0.0 264,425 + Pf1N1B4_214 0.37 +1.3 264,426 - Pf1N1B4_214 0.37 -1.0 264,438 - +2.5 264,438 - Pf1N1B4_214 0.37 +1.9 264,612 + Pf1N1B4_214 0.40 +2.7 264,653 + Pf1N1B4_214 0.41 +1.5 264,653 + -1.5 264,654 - Pf1N1B4_214 0.41 +0.0 264,654 - Pf1N1B4_214 0.41 +0.5 264,660 - Pf1N1B4_214 0.41 -0.1 264,963 + Pf1N1B4_214 0.47 -2.7 265,121 + Pf1N1B4_214 0.50 -1.7 265,122 - Pf1N1B4_214 0.50 -0.4 265,181 + Pf1N1B4_214 0.51 -1.1 265,181 + -0.8 265,182 - Pf1N1B4_214 0.51 -1.0 265,182 - Pf1N1B4_214 0.51 -1.0 265,403 + Pf1N1B4_214 0.55 +0.0 265,404 - Pf1N1B4_214 0.55 -2.3 265,571 + Pf1N1B4_214 0.58 +0.5 265,571 + Pf1N1B4_214 0.58 -1.1 265,571 + Pf1N1B4_214 0.58 -0.8 265,572 - +2.2 265,572 - Pf1N1B4_214 0.58 +3.0 265,572 - -0.8 265,572 - Pf1N1B4_214 0.58 -1.5 265,574 + Pf1N1B4_214 0.58 -0.6 265,784 + Pf1N1B4_214 0.62 -2.4 265,865 + Pf1N1B4_214 0.63 -0.2 265,992 - Pf1N1B4_214 0.65 +2.4 265,992 - -1.5 266,278 + Pf1N1B4_214 0.71 +0.4 266,279 - Pf1N1B4_214 0.71 -2.6 266,279 - Pf1N1B4_214 0.71 -2.2 266,279 - Pf1N1B4_214 0.71 -2.7 266,280 - Pf1N1B4_214 0.71 -0.1 266,566 + Pf1N1B4_214 0.76 -2.0 267,293 + Pf1N1B4_214 0.89 -0.8 267,293 + Pf1N1B4_214 0.89 -2.4 267,293 + Pf1N1B4_214 0.89 -0.6 267,294 - Pf1N1B4_214 0.89 +1.3 267,404 + +2.0 267,404 + +2.3 267,405 - -0.6 267,644 + -2.0 267,795 - +1.7 267,795 - -2.7 267,848 + -0.0 267,848 + +1.3 267,848 + -0.4 267,849 - +4.4 267,849 - -0.2 267,959 - -0.4 268,102 + +1.0 268,102 + +0.4 268,102 + +1.2 268,134 - +1.1 268,258 + -1.2 268,259 - -0.1 268,259 - +1.0 268,381 + -0.9 268,390 + -0.1 268,416 - -0.4 268,564 + +1.0 268,564 + +1.1 268,564 + +0.5 268,565 - -1.2 268,565 - +0.6 268,606 + -0.3 268,607 - -3.1 268,607 - +0.6 268,607 - +2.8
Or see this region's nucleotide sequence