Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c07940 and PGA1_c07950 are separated by 149 nucleotides PGA1_c07950 and PGA1_c07960 are separated by 1 nucleotides
PGA1_c07940: PGA1_c07940 - Uncharacterized protein conserved in bacteria, at 824,056 to 825,033
_c07940
PGA1_c07950: PGA1_c07950 - hypothetical protein, at 825,183 to 825,677
_c07950
PGA1_c07960: PGA1_c07960 - putative integral membrane protein, at 825,679 to 827,196
_c07960
Position (kb) 825
826 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 824.190 kb on + strand, within PGA1_c07940 at 824.231 kb on - strand, within PGA1_c07940 at 824.281 kb on + strand, within PGA1_c07940 at 824.310 kb on + strand, within PGA1_c07940 at 824.350 kb on - strand, within PGA1_c07940 at 824.587 kb on + strand, within PGA1_c07940 at 824.613 kb on - strand, within PGA1_c07940 at 824.674 kb on + strand, within PGA1_c07940 at 824.751 kb on - strand, within PGA1_c07940 at 824.755 kb on + strand, within PGA1_c07940 at 824.760 kb on + strand, within PGA1_c07940 at 824.776 kb on + strand, within PGA1_c07940 at 824.937 kb on + strand at 824.952 kb on - strand at 825.034 kb on + strand at 825.050 kb on - strand at 825.334 kb on - strand, within PGA1_c07950 at 825.397 kb on - strand, within PGA1_c07950 at 825.443 kb on + strand, within PGA1_c07950 at 825.631 kb on + strand at 825.664 kb on + strand at 825.908 kb on + strand, within PGA1_c07960 at 825.916 kb on - strand, within PGA1_c07960 at 825.992 kb on - strand, within PGA1_c07960 at 825.992 kb on - strand, within PGA1_c07960 at 826.040 kb on + strand, within PGA1_c07960 at 826.057 kb on - strand, within PGA1_c07960 at 826.080 kb on + strand, within PGA1_c07960 at 826.098 kb on + strand, within PGA1_c07960 at 826.188 kb on + strand, within PGA1_c07960 at 826.218 kb on - strand, within PGA1_c07960 at 826.220 kb on + strand, within PGA1_c07960 at 826.224 kb on + strand, within PGA1_c07960 at 826.224 kb on + strand, within PGA1_c07960 at 826.224 kb on + strand, within PGA1_c07960 at 826.224 kb on + strand, within PGA1_c07960 at 826.232 kb on - strand, within PGA1_c07960 at 826.232 kb on - strand, within PGA1_c07960 at 826.232 kb on - strand, within PGA1_c07960 at 826.232 kb on - strand, within PGA1_c07960 at 826.309 kb on - strand, within PGA1_c07960 at 826.309 kb on - strand, within PGA1_c07960 at 826.334 kb on - strand, within PGA1_c07960 at 826.407 kb on + strand, within PGA1_c07960 at 826.520 kb on + strand, within PGA1_c07960 at 826.525 kb on + strand, within PGA1_c07960 at 826.538 kb on - strand, within PGA1_c07960
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 824,190 + PGA1_c07940 0.14 +3.5 824,231 - PGA1_c07940 0.18 +0.5 824,281 + PGA1_c07940 0.23 -0.8 824,310 + PGA1_c07940 0.26 +0.2 824,350 - PGA1_c07940 0.30 -0.3 824,587 + PGA1_c07940 0.54 +0.4 824,613 - PGA1_c07940 0.57 -1.2 824,674 + PGA1_c07940 0.63 +0.9 824,751 - PGA1_c07940 0.71 +0.3 824,755 + PGA1_c07940 0.71 -1.9 824,760 + PGA1_c07940 0.72 +0.9 824,776 + PGA1_c07940 0.74 +0.4 824,937 + -0.3 824,952 - -0.7 825,034 + +2.2 825,050 - +1.0 825,334 - PGA1_c07950 0.31 -0.3 825,397 - PGA1_c07950 0.43 -1.9 825,443 + PGA1_c07950 0.53 -2.3 825,631 + +1.5 825,664 + +1.2 825,908 + PGA1_c07960 0.15 -1.1 825,916 - PGA1_c07960 0.16 -0.3 825,992 - PGA1_c07960 0.21 +1.3 825,992 - PGA1_c07960 0.21 +2.3 826,040 + PGA1_c07960 0.24 +0.6 826,057 - PGA1_c07960 0.25 -0.2 826,080 + PGA1_c07960 0.26 -0.0 826,098 + PGA1_c07960 0.28 +0.3 826,188 + PGA1_c07960 0.34 -1.1 826,218 - PGA1_c07960 0.36 +2.0 826,220 + PGA1_c07960 0.36 -1.0 826,224 + PGA1_c07960 0.36 -1.6 826,224 + PGA1_c07960 0.36 -2.4 826,224 + PGA1_c07960 0.36 -1.4 826,224 + PGA1_c07960 0.36 -0.1 826,232 - PGA1_c07960 0.36 -0.6 826,232 - PGA1_c07960 0.36 +0.4 826,232 - PGA1_c07960 0.36 +0.1 826,232 - PGA1_c07960 0.36 -0.9 826,309 - PGA1_c07960 0.42 +0.1 826,309 - PGA1_c07960 0.42 +0.0 826,334 - PGA1_c07960 0.43 +2.0 826,407 + PGA1_c07960 0.48 -1.6 826,520 + PGA1_c07960 0.55 -1.0 826,525 + PGA1_c07960 0.56 +2.1 826,538 - PGA1_c07960 0.57 -1.3
Or see this region's nucleotide sequence