Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p01810 and PGA1_262p01820 overlap by 4 nucleotides PGA1_262p01820 and PGA1_262p01830 are separated by 551 nucleotides
PGA1_262p01810: PGA1_262p01810 - hypothetical protein, at 198,551 to 200,254
_262p01810
PGA1_262p01820: PGA1_262p01820 - Protein of unknown function (DUF3726)., at 200,251 to 200,901
_262p01820
PGA1_262p01830: PGA1_262p01830 - putative aminomethyltransferase, at 201,453 to 202,559
_262p01830
Position (kb) 200
201 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 199.280 kb on - strand, within PGA1_262p01810 at 199.280 kb on - strand, within PGA1_262p01810 at 199.280 kb on - strand, within PGA1_262p01810 at 199.440 kb on - strand, within PGA1_262p01810 at 199.475 kb on + strand, within PGA1_262p01810 at 199.478 kb on - strand, within PGA1_262p01810 at 199.484 kb on - strand, within PGA1_262p01810 at 199.488 kb on - strand, within PGA1_262p01810 at 199.541 kb on + strand, within PGA1_262p01810 at 199.541 kb on + strand, within PGA1_262p01810 at 199.544 kb on - strand, within PGA1_262p01810 at 199.586 kb on - strand, within PGA1_262p01810 at 199.791 kb on + strand, within PGA1_262p01810 at 199.962 kb on + strand, within PGA1_262p01810 at 200.067 kb on + strand, within PGA1_262p01810 at 200.067 kb on + strand, within PGA1_262p01810 at 200.075 kb on - strand, within PGA1_262p01810 at 200.075 kb on - strand, within PGA1_262p01810 at 200.075 kb on - strand, within PGA1_262p01810 at 200.075 kb on - strand, within PGA1_262p01810 at 200.075 kb on - strand, within PGA1_262p01810 at 200.075 kb on - strand, within PGA1_262p01810 at 200.081 kb on + strand, within PGA1_262p01810 at 200.185 kb on + strand at 200.308 kb on + strand at 200.313 kb on + strand at 200.321 kb on - strand, within PGA1_262p01820 at 200.418 kb on + strand, within PGA1_262p01820 at 200.433 kb on + strand, within PGA1_262p01820 at 200.737 kb on - strand, within PGA1_262p01820 at 200.761 kb on + strand, within PGA1_262p01820 at 200.761 kb on + strand, within PGA1_262p01820 at 200.773 kb on + strand, within PGA1_262p01820 at 200.786 kb on - strand, within PGA1_262p01820 at 200.872 kb on - strand at 200.905 kb on + strand at 200.905 kb on + strand at 200.905 kb on + strand at 200.913 kb on - strand at 200.918 kb on - strand at 200.923 kb on + strand at 200.977 kb on + strand at 200.977 kb on + strand at 200.977 kb on + strand at 200.985 kb on - strand at 200.985 kb on - strand at 200.985 kb on - strand at 200.992 kb on + strand at 200.999 kb on + strand at 201.073 kb on + strand at 201.074 kb on - strand at 201.074 kb on - strand at 201.081 kb on - strand at 201.082 kb on - strand at 201.112 kb on - strand at 201.135 kb on + strand at 201.181 kb on + strand at 201.254 kb on + strand at 201.257 kb on + strand at 201.320 kb on + strand at 201.320 kb on + strand at 201.455 kb on - strand at 201.465 kb on + strand at 201.478 kb on - strand at 201.483 kb on - strand at 201.494 kb on + strand at 201.494 kb on + strand at 201.502 kb on - strand at 201.502 kb on - strand at 201.518 kb on - strand at 201.595 kb on + strand, within PGA1_262p01830 at 201.595 kb on + strand, within PGA1_262p01830 at 201.674 kb on - strand, within PGA1_262p01830 at 201.784 kb on + strand, within PGA1_262p01830 at 201.838 kb on + strand, within PGA1_262p01830 at 201.859 kb on - strand, within PGA1_262p01830 at 201.859 kb on - strand, within PGA1_262p01830 at 201.867 kb on + strand, within PGA1_262p01830 at 201.879 kb on - strand, within PGA1_262p01830 at 201.884 kb on + strand, within PGA1_262p01830 at 201.893 kb on - strand, within PGA1_262p01830 at 201.899 kb on + strand, within PGA1_262p01830 at 201.900 kb on + strand, within PGA1_262p01830
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 199,280 - PGA1_262p01810 0.43 +2.0 199,280 - PGA1_262p01810 0.43 -0.1 199,280 - PGA1_262p01810 0.43 -1.4 199,440 - PGA1_262p01810 0.52 +1.0 199,475 + PGA1_262p01810 0.54 -1.0 199,478 - PGA1_262p01810 0.54 -0.3 199,484 - PGA1_262p01810 0.55 -1.1 199,488 - PGA1_262p01810 0.55 +0.9 199,541 + PGA1_262p01810 0.58 +0.4 199,541 + PGA1_262p01810 0.58 +1.3 199,544 - PGA1_262p01810 0.58 -2.8 199,586 - PGA1_262p01810 0.61 -0.3 199,791 + PGA1_262p01810 0.73 +0.5 199,962 + PGA1_262p01810 0.83 -0.9 200,067 + PGA1_262p01810 0.89 -0.3 200,067 + PGA1_262p01810 0.89 +0.4 200,075 - PGA1_262p01810 0.89 +0.6 200,075 - PGA1_262p01810 0.89 +0.4 200,075 - PGA1_262p01810 0.89 +0.8 200,075 - PGA1_262p01810 0.89 -0.2 200,075 - PGA1_262p01810 0.89 -0.4 200,075 - PGA1_262p01810 0.89 +0.7 200,081 + PGA1_262p01810 0.90 +2.7 200,185 + -0.7 200,308 + -0.9 200,313 + +0.9 200,321 - PGA1_262p01820 0.11 +1.6 200,418 + PGA1_262p01820 0.26 +1.6 200,433 + PGA1_262p01820 0.28 +1.3 200,737 - PGA1_262p01820 0.75 +1.3 200,761 + PGA1_262p01820 0.78 -0.4 200,761 + PGA1_262p01820 0.78 -1.2 200,773 + PGA1_262p01820 0.80 +0.6 200,786 - PGA1_262p01820 0.82 +1.1 200,872 - +0.3 200,905 + +0.1 200,905 + +1.2 200,905 + +1.5 200,913 - +1.9 200,918 - +1.3 200,923 + +0.1 200,977 + +0.2 200,977 + +0.3 200,977 + +0.3 200,985 - -1.2 200,985 - +0.3 200,985 - +0.7 200,992 + +1.2 200,999 + +0.8 201,073 + -0.4 201,074 - -2.7 201,074 - -0.1 201,081 - -0.5 201,082 - +0.1 201,112 - -0.4 201,135 + +1.1 201,181 + +0.4 201,254 + +0.9 201,257 + -0.3 201,320 + -1.1 201,320 + +0.3 201,455 - -1.0 201,465 + -0.4 201,478 - +0.3 201,483 - +0.2 201,494 + -0.3 201,494 + -1.3 201,502 - +0.9 201,502 - +0.7 201,518 - -0.3 201,595 + PGA1_262p01830 0.13 +0.3 201,595 + PGA1_262p01830 0.13 +0.6 201,674 - PGA1_262p01830 0.20 +0.0 201,784 + PGA1_262p01830 0.30 -0.9 201,838 + PGA1_262p01830 0.35 +0.1 201,859 - PGA1_262p01830 0.37 -0.9 201,859 - PGA1_262p01830 0.37 +0.1 201,867 + PGA1_262p01830 0.37 -0.7 201,879 - PGA1_262p01830 0.38 -1.5 201,884 + PGA1_262p01830 0.39 -1.3 201,893 - PGA1_262p01830 0.40 +2.3 201,899 + PGA1_262p01830 0.40 +0.2 201,900 + PGA1_262p01830 0.40 +0.1
Or see this region's nucleotide sequence