Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c27660 and PGA1_c27670 are separated by 59 nucleotides PGA1_c27670 and PGA1_c27680 are separated by 103 nucleotides PGA1_c27680 and PGA1_c27690 overlap by 4 nucleotides PGA1_c27690 and PGA1_c27700 are separated by 354 nucleotides
PGA1_c27660: PGA1_c27660 - hypothetical protein, at 2,884,049 to 2,884,240
_c27660
PGA1_c27670: PGA1_c27670 - cytoplasmic chaperon TorD-like protein, at 2,884,300 to 2,884,941
_c27670
PGA1_c27680: PGA1_c27680 - Protein of unknown function (DUF3306)., at 2,885,045 to 2,885,755
_c27680
PGA1_c27690: PGA1_c27690 - Protein of unknown function (DUF3305)., at 2,885,752 to 2,886,327
_c27690
PGA1_c27700: PGA1_c27700 - hypothetical protein, at 2,886,682 to 2,888,631
_c27700
Position (kb)
2885
2886 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2884.102 kb on + strand, within PGA1_c27660 at 2884.174 kb on + strand, within PGA1_c27660 at 2884.174 kb on + strand, within PGA1_c27660 at 2884.219 kb on - strand, within PGA1_c27660 at 2884.301 kb on - strand at 2884.311 kb on + strand at 2884.368 kb on - strand, within PGA1_c27670 at 2884.378 kb on + strand, within PGA1_c27670 at 2884.401 kb on - strand, within PGA1_c27670 at 2884.488 kb on + strand, within PGA1_c27670 at 2884.496 kb on - strand, within PGA1_c27670 at 2884.569 kb on - strand, within PGA1_c27670 at 2884.582 kb on - strand, within PGA1_c27670 at 2884.657 kb on + strand, within PGA1_c27670 at 2884.659 kb on - strand, within PGA1_c27670 at 2884.665 kb on - strand, within PGA1_c27670 at 2884.665 kb on - strand, within PGA1_c27670 at 2884.665 kb on - strand, within PGA1_c27670 at 2884.665 kb on - strand, within PGA1_c27670 at 2885.069 kb on + strand at 2885.126 kb on - strand, within PGA1_c27680 at 2885.154 kb on + strand, within PGA1_c27680 at 2885.197 kb on + strand, within PGA1_c27680 at 2885.348 kb on - strand, within PGA1_c27680 at 2885.385 kb on + strand, within PGA1_c27680 at 2885.385 kb on + strand, within PGA1_c27680 at 2885.385 kb on + strand, within PGA1_c27680 at 2885.412 kb on + strand, within PGA1_c27680 at 2885.412 kb on + strand, within PGA1_c27680 at 2885.412 kb on + strand, within PGA1_c27680 at 2885.412 kb on + strand, within PGA1_c27680 at 2885.420 kb on - strand, within PGA1_c27680 at 2885.420 kb on - strand, within PGA1_c27680 at 2885.420 kb on - strand, within PGA1_c27680 at 2885.534 kb on - strand, within PGA1_c27680 at 2885.753 kb on + strand at 2885.758 kb on - strand at 2885.758 kb on - strand at 2885.758 kb on - strand at 2885.761 kb on - strand at 2885.773 kb on + strand at 2885.787 kb on + strand at 2885.820 kb on + strand, within PGA1_c27690 at 2885.828 kb on - strand, within PGA1_c27690 at 2885.830 kb on + strand, within PGA1_c27690 at 2885.853 kb on + strand, within PGA1_c27690 at 2885.922 kb on - strand, within PGA1_c27690 at 2885.980 kb on + strand, within PGA1_c27690 at 2885.980 kb on + strand, within PGA1_c27690 at 2885.983 kb on - strand, within PGA1_c27690 at 2885.983 kb on - strand, within PGA1_c27690 at 2885.983 kb on - strand, within PGA1_c27690 at 2885.988 kb on - strand, within PGA1_c27690 at 2885.988 kb on - strand, within PGA1_c27690 at 2885.988 kb on - strand, within PGA1_c27690 at 2885.999 kb on - strand, within PGA1_c27690 at 2886.003 kb on - strand, within PGA1_c27690 at 2886.033 kb on + strand, within PGA1_c27690 at 2886.041 kb on - strand, within PGA1_c27690 at 2886.054 kb on + strand, within PGA1_c27690 at 2886.062 kb on - strand, within PGA1_c27690 at 2886.062 kb on - strand, within PGA1_c27690 at 2886.062 kb on - strand, within PGA1_c27690 at 2886.090 kb on - strand, within PGA1_c27690 at 2886.100 kb on + strand, within PGA1_c27690 at 2886.108 kb on - strand, within PGA1_c27690 at 2886.126 kb on - strand, within PGA1_c27690 at 2886.200 kb on + strand, within PGA1_c27690 at 2886.247 kb on + strand, within PGA1_c27690 at 2886.260 kb on - strand, within PGA1_c27690 at 2886.270 kb on + strand at 2886.278 kb on + strand at 2886.297 kb on + strand at 2886.297 kb on + strand at 2886.297 kb on + strand at 2886.307 kb on + strand at 2886.317 kb on - strand at 2886.317 kb on - strand at 2886.325 kb on - strand at 2886.335 kb on - strand at 2886.335 kb on - strand at 2886.336 kb on - strand at 2886.336 kb on - strand at 2886.356 kb on + strand at 2886.356 kb on + strand at 2886.364 kb on + strand at 2886.367 kb on + strand at 2886.372 kb on + strand at 2886.372 kb on - strand at 2886.380 kb on - strand at 2886.454 kb on - strand at 2886.456 kb on + strand at 2886.456 kb on + strand at 2886.456 kb on + strand at 2886.456 kb on + strand at 2886.456 kb on + strand at 2886.456 kb on + strand at 2886.456 kb on + strand at 2886.464 kb on - strand at 2886.464 kb on - strand at 2886.464 kb on - strand at 2886.464 kb on - strand at 2886.464 kb on - strand at 2886.464 kb on - strand at 2886.502 kb on + strand at 2886.502 kb on + strand at 2886.503 kb on + strand at 2886.510 kb on - strand at 2886.510 kb on - strand at 2886.510 kb on - strand at 2886.510 kb on - strand at 2886.510 kb on - strand at 2886.511 kb on - strand at 2886.511 kb on - strand at 2886.511 kb on - strand at 2886.557 kb on + strand at 2886.565 kb on - strand at 2886.572 kb on + strand at 2886.572 kb on + strand at 2886.580 kb on - strand at 2886.580 kb on - strand at 2886.580 kb on - strand at 2886.593 kb on + strand at 2886.610 kb on + strand at 2886.639 kb on + strand at 2886.639 kb on + strand at 2886.649 kb on + strand at 2886.752 kb on + strand at 2886.752 kb on + strand at 2886.752 kb on + strand at 2886.752 kb on + strand at 2886.752 kb on + strand at 2886.752 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 2,884,102 + PGA1_c27660 0.28 +0.3 2,884,174 + PGA1_c27660 0.65 -0.4 2,884,174 + PGA1_c27660 0.65 +1.3 2,884,219 - PGA1_c27660 0.89 -0.4 2,884,301 - -0.8 2,884,311 + +0.7 2,884,368 - PGA1_c27670 0.11 +0.1 2,884,378 + PGA1_c27670 0.12 -0.6 2,884,401 - PGA1_c27670 0.16 +0.6 2,884,488 + PGA1_c27670 0.29 -0.6 2,884,496 - PGA1_c27670 0.31 +0.4 2,884,569 - PGA1_c27670 0.42 -1.3 2,884,582 - PGA1_c27670 0.44 +1.1 2,884,657 + PGA1_c27670 0.56 -0.2 2,884,659 - PGA1_c27670 0.56 -0.8 2,884,665 - PGA1_c27670 0.57 -0.8 2,884,665 - PGA1_c27670 0.57 -1.3 2,884,665 - PGA1_c27670 0.57 +1.7 2,884,665 - PGA1_c27670 0.57 +0.2 2,885,069 + -3.3 2,885,126 - PGA1_c27680 0.11 +1.7 2,885,154 + PGA1_c27680 0.15 +0.7 2,885,197 + PGA1_c27680 0.21 -0.3 2,885,348 - PGA1_c27680 0.43 +0.3 2,885,385 + PGA1_c27680 0.48 +1.4 2,885,385 + PGA1_c27680 0.48 +0.4 2,885,385 + PGA1_c27680 0.48 +2.7 2,885,412 + PGA1_c27680 0.52 +0.9 2,885,412 + PGA1_c27680 0.52 -1.5 2,885,412 + PGA1_c27680 0.52 +0.2 2,885,412 + PGA1_c27680 0.52 -0.6 2,885,420 - PGA1_c27680 0.53 +1.4 2,885,420 - PGA1_c27680 0.53 +1.7 2,885,420 - PGA1_c27680 0.53 -0.6 2,885,534 - PGA1_c27680 0.69 +0.5 2,885,753 + -0.5 2,885,758 - -0.6 2,885,758 - -1.6 2,885,758 - +0.4 2,885,761 - +0.7 2,885,773 + +1.1 2,885,787 + -1.1 2,885,820 + PGA1_c27690 0.12 -1.3 2,885,828 - PGA1_c27690 0.13 +0.7 2,885,830 + PGA1_c27690 0.14 +1.7 2,885,853 + PGA1_c27690 0.18 +1.1 2,885,922 - PGA1_c27690 0.30 -0.9 2,885,980 + PGA1_c27690 0.40 -1.9 2,885,980 + PGA1_c27690 0.40 -1.1 2,885,983 - PGA1_c27690 0.40 +0.4 2,885,983 - PGA1_c27690 0.40 -1.3 2,885,983 - PGA1_c27690 0.40 +1.2 2,885,988 - PGA1_c27690 0.41 +0.2 2,885,988 - PGA1_c27690 0.41 -1.4 2,885,988 - PGA1_c27690 0.41 -0.9 2,885,999 - PGA1_c27690 0.43 -0.7 2,886,003 - PGA1_c27690 0.44 +0.8 2,886,033 + PGA1_c27690 0.49 -0.8 2,886,041 - PGA1_c27690 0.50 +0.3 2,886,054 + PGA1_c27690 0.52 +1.9 2,886,062 - PGA1_c27690 0.54 -0.3 2,886,062 - PGA1_c27690 0.54 +0.6 2,886,062 - PGA1_c27690 0.54 +0.2 2,886,090 - PGA1_c27690 0.59 +1.6 2,886,100 + PGA1_c27690 0.60 +0.2 2,886,108 - PGA1_c27690 0.62 +0.7 2,886,126 - PGA1_c27690 0.65 +0.2 2,886,200 + PGA1_c27690 0.78 -0.5 2,886,247 + PGA1_c27690 0.86 +1.5 2,886,260 - PGA1_c27690 0.88 -1.1 2,886,270 + +0.9 2,886,278 + +0.1 2,886,297 + +1.1 2,886,297 + -0.1 2,886,297 + -1.4 2,886,307 + +0.7 2,886,317 - +2.3 2,886,317 - -0.5 2,886,325 - +0.6 2,886,335 - -0.0 2,886,335 - +1.1 2,886,336 - +1.5 2,886,336 - +0.6 2,886,356 + +0.0 2,886,356 + +0.1 2,886,364 + -0.5 2,886,367 + -0.8 2,886,372 + -0.4 2,886,372 - -0.1 2,886,380 - -1.0 2,886,454 - -0.3 2,886,456 + -1.0 2,886,456 + -0.1 2,886,456 + -0.9 2,886,456 + -0.7 2,886,456 + +0.7 2,886,456 + +0.7 2,886,456 + +1.7 2,886,464 - +1.2 2,886,464 - -0.3 2,886,464 - +0.4 2,886,464 - -1.1 2,886,464 - -0.3 2,886,464 - -0.3 2,886,502 + +0.3 2,886,502 + +0.7 2,886,503 + -1.5 2,886,510 - +0.5 2,886,510 - -1.4 2,886,510 - -2.6 2,886,510 - +0.2 2,886,510 - +0.6 2,886,511 - +0.2 2,886,511 - +0.4 2,886,511 - -0.1 2,886,557 + +0.7 2,886,565 - -0.3 2,886,572 + -0.6 2,886,572 + -1.2 2,886,580 - -0.6 2,886,580 - +1.6 2,886,580 - +1.7 2,886,593 + +0.1 2,886,610 + +0.5 2,886,639 + -0.5 2,886,639 + -1.7 2,886,649 + +0.3 2,886,752 + +1.1 2,886,752 + +1.1 2,886,752 + +0.9 2,886,752 + +1.3 2,886,752 + +0.5 2,886,752 + +0.7
Or see this region's nucleotide sequence