Experiment: Phaeo_ML1_MIT diversity barseq
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c17820 and PGA1_c17830 are separated by 48 nucleotides PGA1_c17830 and PGA1_c17840 are separated by 35 nucleotides PGA1_c17840 and PGA1_c17850 overlap by 8 nucleotides
PGA1_c17820: PGA1_c17820 - gene transfer agent major capsid protein, at 1,862,932 to 1,864,116
_c17820
PGA1_c17830: PGA1_c17830 - putative gene transfer agent prohead protease, at 1,864,165 to 1,864,782
_c17830
PGA1_c17840: PGA1_c17840 - hypothetical protein, at 1,864,818 to 1,865,036
_c17840
PGA1_c17850: PGA1_c17850 - putative gene transfer agent portal protein, at 1,865,029 to 1,866,222
_c17850
Position (kb) 1864
1865 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1863.251 kb on + strand, within PGA1_c17820 at 1863.251 kb on + strand, within PGA1_c17820 at 1863.302 kb on + strand, within PGA1_c17820 at 1863.451 kb on + strand, within PGA1_c17820 at 1863.498 kb on + strand, within PGA1_c17820 at 1863.506 kb on - strand, within PGA1_c17820 at 1863.551 kb on + strand, within PGA1_c17820 at 1863.551 kb on + strand, within PGA1_c17820 at 1863.559 kb on - strand, within PGA1_c17820 at 1863.654 kb on - strand, within PGA1_c17820 at 1863.678 kb on + strand, within PGA1_c17820 at 1863.693 kb on + strand, within PGA1_c17820 at 1863.734 kb on + strand, within PGA1_c17820 at 1863.734 kb on + strand, within PGA1_c17820 at 1863.917 kb on - strand, within PGA1_c17820 at 1863.964 kb on + strand, within PGA1_c17820 at 1863.965 kb on + strand, within PGA1_c17820 at 1863.965 kb on + strand, within PGA1_c17820 at 1863.965 kb on + strand, within PGA1_c17820 at 1863.973 kb on - strand, within PGA1_c17820 at 1863.973 kb on - strand, within PGA1_c17820 at 1863.973 kb on - strand, within PGA1_c17820 at 1863.973 kb on - strand, within PGA1_c17820 at 1863.973 kb on - strand, within PGA1_c17820 at 1863.973 kb on - strand, within PGA1_c17820 at 1864.015 kb on - strand at 1864.083 kb on - strand at 1864.099 kb on + strand at 1864.107 kb on - strand at 1864.161 kb on + strand at 1864.176 kb on - strand at 1864.177 kb on + strand at 1864.210 kb on - strand at 1864.254 kb on - strand, within PGA1_c17830 at 1864.283 kb on + strand, within PGA1_c17830 at 1864.445 kb on + strand, within PGA1_c17830 at 1864.547 kb on + strand, within PGA1_c17830 at 1864.611 kb on + strand, within PGA1_c17830 at 1864.701 kb on - strand, within PGA1_c17830 at 1864.726 kb on + strand at 1864.872 kb on + strand, within PGA1_c17840 at 1864.994 kb on + strand, within PGA1_c17840 at 1865.198 kb on - strand, within PGA1_c17850 at 1865.244 kb on - strand, within PGA1_c17850 at 1865.337 kb on + strand, within PGA1_c17850 at 1865.345 kb on - strand, within PGA1_c17850 at 1865.529 kb on + strand, within PGA1_c17850 at 1865.538 kb on - strand, within PGA1_c17850 at 1865.538 kb on - strand, within PGA1_c17850 at 1865.549 kb on + strand, within PGA1_c17850 at 1865.557 kb on - strand, within PGA1_c17850 at 1865.557 kb on - strand, within PGA1_c17850 at 1865.564 kb on + strand, within PGA1_c17850 at 1865.595 kb on + strand, within PGA1_c17850 at 1865.603 kb on - strand, within PGA1_c17850 at 1865.603 kb on - strand, within PGA1_c17850 at 1865.618 kb on - strand, within PGA1_c17850 at 1865.682 kb on + strand, within PGA1_c17850 at 1865.682 kb on + strand, within PGA1_c17850 at 1865.727 kb on + strand, within PGA1_c17850
Per-strain Table
Position Strand Gene LocusTag Fraction Phaeo_ML1_MIT diversity barseq remove 1,863,251 + PGA1_c17820 0.27 +0.6 1,863,251 + PGA1_c17820 0.27 +0.6 1,863,302 + PGA1_c17820 0.31 +2.0 1,863,451 + PGA1_c17820 0.44 -1.0 1,863,498 + PGA1_c17820 0.48 +1.5 1,863,506 - PGA1_c17820 0.48 -1.5 1,863,551 + PGA1_c17820 0.52 +1.9 1,863,551 + PGA1_c17820 0.52 -0.1 1,863,559 - PGA1_c17820 0.53 +1.8 1,863,654 - PGA1_c17820 0.61 +0.6 1,863,678 + PGA1_c17820 0.63 -0.4 1,863,693 + PGA1_c17820 0.64 -0.2 1,863,734 + PGA1_c17820 0.68 +0.6 1,863,734 + PGA1_c17820 0.68 -0.1 1,863,917 - PGA1_c17820 0.83 -0.6 1,863,964 + PGA1_c17820 0.87 -0.0 1,863,965 + PGA1_c17820 0.87 -0.9 1,863,965 + PGA1_c17820 0.87 +0.0 1,863,965 + PGA1_c17820 0.87 -1.0 1,863,973 - PGA1_c17820 0.88 +1.9 1,863,973 - PGA1_c17820 0.88 +0.1 1,863,973 - PGA1_c17820 0.88 -0.6 1,863,973 - PGA1_c17820 0.88 -0.3 1,863,973 - PGA1_c17820 0.88 +0.0 1,863,973 - PGA1_c17820 0.88 +0.5 1,864,015 - -0.5 1,864,083 - -0.0 1,864,099 + +0.0 1,864,107 - -0.1 1,864,161 + +0.5 1,864,176 - -1.1 1,864,177 + -0.6 1,864,210 - -0.5 1,864,254 - PGA1_c17830 0.14 -0.0 1,864,283 + PGA1_c17830 0.19 -1.7 1,864,445 + PGA1_c17830 0.45 -1.2 1,864,547 + PGA1_c17830 0.62 +0.2 1,864,611 + PGA1_c17830 0.72 -1.1 1,864,701 - PGA1_c17830 0.87 -1.7 1,864,726 + -0.4 1,864,872 + PGA1_c17840 0.25 -0.4 1,864,994 + PGA1_c17840 0.80 -0.9 1,865,198 - PGA1_c17850 0.14 +0.0 1,865,244 - PGA1_c17850 0.18 -1.1 1,865,337 + PGA1_c17850 0.26 +0.3 1,865,345 - PGA1_c17850 0.26 +0.4 1,865,529 + PGA1_c17850 0.42 +1.1 1,865,538 - PGA1_c17850 0.43 +1.5 1,865,538 - PGA1_c17850 0.43 -1.6 1,865,549 + PGA1_c17850 0.44 +0.0 1,865,557 - PGA1_c17850 0.44 -3.0 1,865,557 - PGA1_c17850 0.44 -0.4 1,865,564 + PGA1_c17850 0.45 -0.5 1,865,595 + PGA1_c17850 0.47 +0.3 1,865,603 - PGA1_c17850 0.48 +0.4 1,865,603 - PGA1_c17850 0.48 +0.2 1,865,618 - PGA1_c17850 0.49 +0.0 1,865,682 + PGA1_c17850 0.55 -1.2 1,865,682 + PGA1_c17850 0.55 +0.8 1,865,727 + PGA1_c17850 0.58 +0.4
Or see this region's nucleotide sequence