Experiment: carbon source Sucrose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_769 and Pf1N1B4_770 are separated by 324 nucleotides Pf1N1B4_770 and Pf1N1B4_771 overlap by 18 nucleotides
Pf1N1B4_769 - Aerobic glycerol-3-phosphate dehydrogenase (EC 1.1.5.3), at 873,701 to 875,161
_769
Pf1N1B4_770 - hypothetical protein, at 875,486 to 875,626
_770
Pf1N1B4_771 - ABC transporter for L-asparagine and L-glutamate, periplasmic substrate-binding component <small>(from data)</small>, at 875,609 to 876,523
_771
Position (kb) 875
876 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 874.589 kb on + strand, within Pf1N1B4_769 at 874.589 kb on + strand, within Pf1N1B4_769 at 874.589 kb on + strand, within Pf1N1B4_769 at 874.589 kb on + strand, within Pf1N1B4_769 at 874.652 kb on + strand, within Pf1N1B4_769 at 874.653 kb on - strand, within Pf1N1B4_769 at 874.766 kb on + strand, within Pf1N1B4_769 at 874.913 kb on + strand, within Pf1N1B4_769 at 874.913 kb on + strand, within Pf1N1B4_769 at 874.913 kb on + strand, within Pf1N1B4_769 at 874.913 kb on + strand, within Pf1N1B4_769 at 874.957 kb on + strand, within Pf1N1B4_769 at 874.991 kb on + strand, within Pf1N1B4_769 at 874.991 kb on + strand, within Pf1N1B4_769 at 875.009 kb on + strand, within Pf1N1B4_769 at 875.009 kb on + strand, within Pf1N1B4_769 at 875.009 kb on + strand, within Pf1N1B4_769 at 875.114 kb on + strand at 875.225 kb on + strand at 875.225 kb on + strand at 875.225 kb on + strand at 875.225 kb on + strand at 875.226 kb on - strand at 875.226 kb on - strand at 875.281 kb on + strand at 875.282 kb on - strand at 875.284 kb on + strand at 875.284 kb on + strand at 875.285 kb on - strand at 875.285 kb on - strand at 875.299 kb on + strand at 875.340 kb on - strand at 875.410 kb on - strand at 875.464 kb on - strand at 875.494 kb on + strand at 875.582 kb on + strand, within Pf1N1B4_770 at 875.582 kb on + strand, within Pf1N1B4_770 at 875.583 kb on - strand, within Pf1N1B4_770 at 875.584 kb on + strand, within Pf1N1B4_770 at 875.584 kb on + strand, within Pf1N1B4_770 at 875.584 kb on + strand, within Pf1N1B4_770 at 875.584 kb on + strand, within Pf1N1B4_770 at 875.762 kb on + strand, within Pf1N1B4_771 at 875.795 kb on + strand, within Pf1N1B4_771 at 875.871 kb on - strand, within Pf1N1B4_771 at 875.871 kb on - strand, within Pf1N1B4_771 at 876.035 kb on + strand, within Pf1N1B4_771 at 876.036 kb on - strand, within Pf1N1B4_771 at 876.036 kb on - strand at 876.036 kb on - strand, within Pf1N1B4_771 at 876.302 kb on + strand, within Pf1N1B4_771 at 876.303 kb on - strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.371 kb on + strand, within Pf1N1B4_771 at 876.372 kb on - strand, within Pf1N1B4_771 at 876.372 kb on - strand, within Pf1N1B4_771 at 876.372 kb on - strand, within Pf1N1B4_771
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 1mM remove 874,589 + Pf1N1B4_769 0.61 -2.6 874,589 + Pf1N1B4_769 0.61 -0.8 874,589 + Pf1N1B4_769 0.61 -1.5 874,589 + Pf1N1B4_769 0.61 -2.3 874,652 + Pf1N1B4_769 0.65 -1.7 874,653 - Pf1N1B4_769 0.65 -0.5 874,766 + Pf1N1B4_769 0.73 -1.4 874,913 + Pf1N1B4_769 0.83 -3.0 874,913 + Pf1N1B4_769 0.83 -1.9 874,913 + Pf1N1B4_769 0.83 -3.1 874,913 + Pf1N1B4_769 0.83 -1.1 874,957 + Pf1N1B4_769 0.86 -1.8 874,991 + Pf1N1B4_769 0.88 -2.8 874,991 + Pf1N1B4_769 0.88 -2.4 875,009 + Pf1N1B4_769 0.90 -3.5 875,009 + Pf1N1B4_769 0.90 -2.2 875,009 + Pf1N1B4_769 0.90 -2.8 875,114 + -0.3 875,225 + -1.8 875,225 + +1.1 875,225 + +0.2 875,225 + +0.0 875,226 - -0.5 875,226 - +0.5 875,281 + -0.6 875,282 - -2.1 875,284 + +0.1 875,284 + -0.0 875,285 - -2.0 875,285 - +0.1 875,299 + -2.7 875,340 - +0.3 875,410 - +0.8 875,464 - -1.6 875,494 + -1.8 875,582 + Pf1N1B4_770 0.68 -2.8 875,582 + Pf1N1B4_770 0.68 -2.3 875,583 - Pf1N1B4_770 0.69 -0.9 875,584 + Pf1N1B4_770 0.70 -1.9 875,584 + Pf1N1B4_770 0.70 -1.5 875,584 + Pf1N1B4_770 0.70 +2.4 875,584 + Pf1N1B4_770 0.70 -2.3 875,762 + Pf1N1B4_771 0.17 +3.6 875,795 + Pf1N1B4_771 0.20 -0.7 875,871 - Pf1N1B4_771 0.29 +0.7 875,871 - Pf1N1B4_771 0.29 +2.5 876,035 + Pf1N1B4_771 0.47 -0.7 876,036 - Pf1N1B4_771 0.47 -2.4 876,036 - +6.3 876,036 - Pf1N1B4_771 0.47 +1.6 876,302 + Pf1N1B4_771 0.76 -2.7 876,303 - Pf1N1B4_771 0.76 +0.7 876,371 + Pf1N1B4_771 0.83 +1.9 876,371 + Pf1N1B4_771 0.83 +2.0 876,371 + Pf1N1B4_771 0.83 +1.7 876,371 + Pf1N1B4_771 0.83 +1.5 876,371 + Pf1N1B4_771 0.83 -0.8 876,371 + Pf1N1B4_771 0.83 +2.6 876,371 + Pf1N1B4_771 0.83 +1.4 876,371 + Pf1N1B4_771 0.83 -0.0 876,371 + Pf1N1B4_771 0.83 -0.4 876,372 - Pf1N1B4_771 0.83 -1.1 876,372 - Pf1N1B4_771 0.83 +2.1 876,372 - Pf1N1B4_771 0.83 +0.6
Or see this region's nucleotide sequence