Experiment: carbon source Sucrose 1mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5499 and Pf1N1B4_5500 are separated by 177 nucleotides Pf1N1B4_5500 and Pf1N1B4_5501 are separated by 209 nucleotides Pf1N1B4_5501 and Pf1N1B4_5502 overlap by 35 nucleotides
Pf1N1B4_5499 - hypothetical protein, at 247,890 to 249,695
_5499
Pf1N1B4_5500 - hypothetical protein, at 249,873 to 250,862
_5500
Pf1N1B4_5501 - hypothetical protein, at 251,072 to 251,413
_5501
Pf1N1B4_5502 - hypothetical protein, at 251,379 to 252,542
_5502
Position (kb)
249
250
251 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 248.900 kb on + strand, within Pf1N1B4_5499 at 248.971 kb on + strand, within Pf1N1B4_5499 at 248.972 kb on - strand, within Pf1N1B4_5499 at 248.972 kb on - strand, within Pf1N1B4_5499 at 248.972 kb on - strand, within Pf1N1B4_5499 at 249.004 kb on - strand at 249.070 kb on + strand, within Pf1N1B4_5499 at 249.070 kb on + strand, within Pf1N1B4_5499 at 249.070 kb on + strand, within Pf1N1B4_5499 at 249.071 kb on - strand, within Pf1N1B4_5499 at 249.232 kb on + strand, within Pf1N1B4_5499 at 249.232 kb on + strand at 249.232 kb on + strand, within Pf1N1B4_5499 at 249.233 kb on - strand, within Pf1N1B4_5499 at 249.233 kb on - strand at 249.246 kb on - strand, within Pf1N1B4_5499 at 249.246 kb on - strand, within Pf1N1B4_5499 at 249.274 kb on + strand, within Pf1N1B4_5499 at 249.450 kb on - strand, within Pf1N1B4_5499 at 249.450 kb on - strand, within Pf1N1B4_5499 at 249.450 kb on - strand, within Pf1N1B4_5499 at 249.560 kb on + strand at 249.571 kb on + strand at 249.571 kb on + strand at 249.571 kb on + strand at 249.572 kb on - strand at 249.572 kb on - strand at 249.623 kb on + strand at 249.627 kb on + strand at 249.627 kb on + strand at 249.627 kb on + strand at 249.628 kb on - strand at 249.682 kb on + strand at 249.683 kb on - strand at 249.683 kb on - strand at 249.742 kb on - strand at 249.840 kb on - strand at 249.840 kb on - strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.880 kb on + strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.881 kb on - strand at 249.882 kb on + strand at 249.883 kb on - strand at 249.883 kb on - strand at 249.883 kb on - strand at 250.144 kb on + strand, within Pf1N1B4_5500 at 250.145 kb on - strand, within Pf1N1B4_5500 at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.224 kb on + strand at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.224 kb on + strand at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.224 kb on + strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.225 kb on - strand, within Pf1N1B4_5500 at 250.268 kb on - strand, within Pf1N1B4_5500 at 250.365 kb on + strand, within Pf1N1B4_5500 at 250.365 kb on + strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.366 kb on - strand, within Pf1N1B4_5500 at 250.429 kb on + strand, within Pf1N1B4_5500 at 250.429 kb on + strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.430 kb on - strand, within Pf1N1B4_5500 at 250.689 kb on - strand, within Pf1N1B4_5500 at 250.689 kb on - strand, within Pf1N1B4_5500 at 250.737 kb on - strand, within Pf1N1B4_5500 at 250.757 kb on - strand at 250.758 kb on + strand, within Pf1N1B4_5500 at 250.758 kb on + strand at 250.758 kb on + strand, within Pf1N1B4_5500 at 250.758 kb on + strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.759 kb on - strand, within Pf1N1B4_5500 at 250.771 kb on - strand at 250.805 kb on - strand at 250.807 kb on + strand at 250.807 kb on + strand at 250.807 kb on + strand at 250.870 kb on + strand at 250.870 kb on + strand at 250.871 kb on - strand at 250.871 kb on - strand at 250.871 kb on - strand at 250.976 kb on + strand at 250.976 kb on + strand at 250.976 kb on + strand at 250.977 kb on - strand at 250.977 kb on - strand at 251.024 kb on + strand at 251.024 kb on + strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.025 kb on - strand at 251.067 kb on + strand at 251.067 kb on + strand at 251.067 kb on + strand at 251.068 kb on - strand at 251.260 kb on + strand, within Pf1N1B4_5501 at 251.261 kb on - strand, within Pf1N1B4_5501 at 251.261 kb on - strand, within Pf1N1B4_5501 at 251.279 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.302 kb on + strand, within Pf1N1B4_5501 at 251.303 kb on - strand, within Pf1N1B4_5501 at 251.303 kb on - strand, within Pf1N1B4_5501 at 251.303 kb on - strand, within Pf1N1B4_5501 at 251.411 kb on + strand at 251.411 kb on + strand at 251.412 kb on - strand at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.517 kb on + strand, within Pf1N1B4_5502 at 251.518 kb on - strand, within Pf1N1B4_5502 at 251.518 kb on - strand, within Pf1N1B4_5502 at 251.534 kb on + strand, within Pf1N1B4_5502 at 251.590 kb on + strand at 251.739 kb on + strand, within Pf1N1B4_5502 at 251.739 kb on + strand, within Pf1N1B4_5502 at 251.739 kb on + strand, within Pf1N1B4_5502 at 251.740 kb on - strand, within Pf1N1B4_5502 at 251.798 kb on + strand, within Pf1N1B4_5502
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 1mM remove 248,900 + Pf1N1B4_5499 0.56 -1.6 248,971 + Pf1N1B4_5499 0.60 +2.0 248,972 - Pf1N1B4_5499 0.60 +0.7 248,972 - Pf1N1B4_5499 0.60 -2.3 248,972 - Pf1N1B4_5499 0.60 +0.9 249,004 - -1.5 249,070 + Pf1N1B4_5499 0.65 -1.6 249,070 + Pf1N1B4_5499 0.65 -2.8 249,070 + Pf1N1B4_5499 0.65 -2.0 249,071 - Pf1N1B4_5499 0.65 +0.4 249,232 + Pf1N1B4_5499 0.74 +1.8 249,232 + -0.9 249,232 + Pf1N1B4_5499 0.74 +0.4 249,233 - Pf1N1B4_5499 0.74 -0.1 249,233 - -0.9 249,246 - Pf1N1B4_5499 0.75 +2.8 249,246 - Pf1N1B4_5499 0.75 +0.9 249,274 + Pf1N1B4_5499 0.77 -1.1 249,450 - Pf1N1B4_5499 0.86 +0.4 249,450 - Pf1N1B4_5499 0.86 -2.6 249,450 - Pf1N1B4_5499 0.86 +1.7 249,560 + -2.0 249,571 + -1.4 249,571 + +0.4 249,571 + -1.6 249,572 - -2.0 249,572 - -1.0 249,623 + -1.1 249,627 + +2.3 249,627 + -1.0 249,627 + -2.7 249,628 - +0.1 249,682 + -1.0 249,683 - -1.0 249,683 - -1.7 249,742 - -1.7 249,840 - +1.8 249,840 - -2.8 249,880 + -2.8 249,880 + -0.6 249,880 + -0.1 249,880 + +0.6 249,880 + +1.9 249,880 + +1.0 249,881 - -0.6 249,881 - -0.3 249,881 - -1.7 249,881 - +0.9 249,881 - -0.6 249,881 - +1.1 249,881 - -0.9 249,881 - +1.1 249,881 - +1.9 249,882 + -3.2 249,883 - -1.9 249,883 - -0.6 249,883 - -2.3 250,144 + Pf1N1B4_5500 0.27 -0.9 250,145 - Pf1N1B4_5500 0.27 -1.1 250,224 + Pf1N1B4_5500 0.35 -1.5 250,224 + +2.4 250,224 + Pf1N1B4_5500 0.35 -1.1 250,224 + -0.9 250,224 + Pf1N1B4_5500 0.35 -2.8 250,224 + Pf1N1B4_5500 0.35 -1.9 250,225 - Pf1N1B4_5500 0.36 +0.3 250,225 - Pf1N1B4_5500 0.36 -2.1 250,225 - Pf1N1B4_5500 0.36 -1.9 250,225 - Pf1N1B4_5500 0.36 -1.3 250,225 - Pf1N1B4_5500 0.36 -1.7 250,225 - Pf1N1B4_5500 0.36 +1.5 250,225 - Pf1N1B4_5500 0.36 -0.4 250,268 - Pf1N1B4_5500 0.40 +0.5 250,365 + Pf1N1B4_5500 0.50 +1.9 250,365 + Pf1N1B4_5500 0.50 +0.9 250,366 - Pf1N1B4_5500 0.50 +2.3 250,366 - Pf1N1B4_5500 0.50 -0.5 250,366 - Pf1N1B4_5500 0.50 -2.3 250,366 - Pf1N1B4_5500 0.50 -0.7 250,429 + Pf1N1B4_5500 0.56 +2.2 250,429 + Pf1N1B4_5500 0.56 -2.9 250,430 - Pf1N1B4_5500 0.56 +2.0 250,430 - Pf1N1B4_5500 0.56 -2.4 250,430 - Pf1N1B4_5500 0.56 +2.7 250,430 - Pf1N1B4_5500 0.56 +1.0 250,689 - Pf1N1B4_5500 0.82 +1.3 250,689 - Pf1N1B4_5500 0.82 +0.0 250,737 - Pf1N1B4_5500 0.87 -1.8 250,757 - +0.4 250,758 + Pf1N1B4_5500 0.89 -1.6 250,758 + -1.5 250,758 + Pf1N1B4_5500 0.89 -1.8 250,758 + Pf1N1B4_5500 0.89 -2.4 250,759 - Pf1N1B4_5500 0.89 -1.3 250,759 - Pf1N1B4_5500 0.89 -2.0 250,759 - Pf1N1B4_5500 0.89 +0.5 250,759 - Pf1N1B4_5500 0.89 -2.3 250,759 - Pf1N1B4_5500 0.89 -2.1 250,759 - Pf1N1B4_5500 0.89 -0.5 250,759 - Pf1N1B4_5500 0.89 -0.9 250,759 - Pf1N1B4_5500 0.89 +1.6 250,771 - -0.9 250,805 - -1.0 250,807 + -2.4 250,807 + -0.9 250,807 + -1.0 250,870 + -1.1 250,870 + -1.3 250,871 - +2.3 250,871 - -0.9 250,871 - -2.1 250,976 + -2.1 250,976 + -0.5 250,976 + +2.4 250,977 - +3.2 250,977 - +2.1 251,024 + +0.3 251,024 + -2.7 251,025 - -0.7 251,025 - -1.8 251,025 - -0.4 251,025 - +0.6 251,025 - -1.7 251,025 - -1.7 251,067 + +2.6 251,067 + -1.0 251,067 + -2.0 251,068 - -0.2 251,260 + Pf1N1B4_5501 0.55 +1.5 251,261 - Pf1N1B4_5501 0.55 -1.7 251,261 - Pf1N1B4_5501 0.55 -1.2 251,279 + Pf1N1B4_5501 0.61 +0.6 251,302 + Pf1N1B4_5501 0.67 -0.1 251,302 + Pf1N1B4_5501 0.67 -0.4 251,302 + Pf1N1B4_5501 0.67 -2.2 251,302 + Pf1N1B4_5501 0.67 -2.4 251,303 - Pf1N1B4_5501 0.68 -2.3 251,303 - Pf1N1B4_5501 0.68 +1.4 251,303 - Pf1N1B4_5501 0.68 -2.4 251,411 + +0.2 251,411 + -1.6 251,412 - -0.8 251,517 + Pf1N1B4_5502 0.12 +0.5 251,517 + Pf1N1B4_5502 0.12 -1.3 251,517 + Pf1N1B4_5502 0.12 -2.6 251,517 + Pf1N1B4_5502 0.12 -1.6 251,517 + Pf1N1B4_5502 0.12 +0.5 251,517 + Pf1N1B4_5502 0.12 -2.7 251,518 - Pf1N1B4_5502 0.12 +0.3 251,518 - Pf1N1B4_5502 0.12 +0.4 251,534 + Pf1N1B4_5502 0.13 +0.2 251,590 + -1.5 251,739 + Pf1N1B4_5502 0.31 -0.7 251,739 + Pf1N1B4_5502 0.31 +1.4 251,739 + Pf1N1B4_5502 0.31 -2.3 251,740 - Pf1N1B4_5502 0.31 -0.4 251,798 + Pf1N1B4_5502 0.36 -2.2
Or see this region's nucleotide sequence